Membrane Filtration Technique for Water Analysis (E. coli, Salmonella, Pseudomonas, Coliform etc.)

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Membrane Filtration Technique for Water Analysis (E. coli, Salmonella, Pseudomonas, Coliform etc.)

Рет қаралды 195,294

MicroChem's Experiments

Күн бұрын

Пікірлер: 259

@dshift2225 3 жыл бұрын

This my job at Nestle :) Living the dream!

@MicroChemsExperiments 3 жыл бұрын

Great !!!

@khushiyadav6723 3 жыл бұрын

Are you from India?

@BhavyaRecordsOfficial 3 жыл бұрын

Can u tell me if there is any vacancy available in Nestle?

@n4nishiiiii 2 жыл бұрын

So you only perform water testing?

@muralibond1373 2 жыл бұрын

what's the package bro...

@me..7885 2 жыл бұрын

Can not get over how amaizing ur explaination is...all r grateful for that massive work 🌹🌹🌹🌹

@MicroChemsExperiments Жыл бұрын

Thanks a bunch

@ucjakozicka978 6 ай бұрын

The explanation was clear, concise and informative, I learnt all I needed to for a project Im working on, thank you!

@MicroChemsExperiments 6 ай бұрын

Thank you so much

@MicroChemsExperiments 6 ай бұрын

Send email for get help/suggestions about your practical knowledge (mail.mic.chem@gmail.com)

@agboalfred4567 2 жыл бұрын

This is beautiful and so easy to comprehend. Thanks a lot

@MicroChemsExperiments 2 жыл бұрын

You are welcome. Stay with us

@01kanan 25 күн бұрын

Good 👍information and steps of Coliform Group E.coli found proper

@01kanan 25 күн бұрын

Please mam red colonies are gram negative bacteria mostly.

@redweasleypotter1127 4 ай бұрын

Thank for this video. So informative and another technique is added to my list

@prafull07prajapati83 2 жыл бұрын

You are talented and delivers the lecture very smooth Thank you so much

@MicroChemsExperiments 2 жыл бұрын

Thank you so much. Stay with us

@materialsandtechnology8956 Жыл бұрын

kzfaq.info/get/bejne/q92Xh5lmqs-9YYU.html watch this video of membrane for water purification

@vsrpinternational3863 5 ай бұрын

Brilliant..Continue the journey of knowledge.

@MicroChemsExperiments 5 ай бұрын

Thank you, I will

@gyss3349 Жыл бұрын

Nice.i. appreciating & thanks all of you for giving me scientific knowledge regarding to microbiology & Micology in general.

@MicroChemsExperiments Жыл бұрын

Our pleasure!

@SauravKumar-hm9un 3 жыл бұрын

Nice video....plz make a video on multiple fermantation tube techniques for fecal coliform .

@nisasa4814 3 жыл бұрын

yess agree👆

@MicroChemsExperiments 3 жыл бұрын

Noted the parameter. We will work on it soon. Stay with us.

@sushantpatil6679 27 күн бұрын

@@MicroChemsExperimentswhen you will upload it?

@rakiborrahman1368 24 күн бұрын

Perfect deliberation...👍 Thank you. May I have your gram negative strain photographic analysis under Florence Microscope please Dr. R.Rehman

@cyrusngullie8678 Жыл бұрын

Very nice, I really like it. just one rectification. It says incubate the plate @35 degree but in the video it showed putting the plate in the hot air oven.

@MicroChemsExperiments Жыл бұрын

We are using the Hot Air Oven as incubator.

@arzoosundrani1415 3 жыл бұрын

Very nice video

@MicroChemsExperiments 3 жыл бұрын

Thank you

@sghosh1234 Ай бұрын

I have a query..Suppose E coli (blue colony) is present in 70 CFU and coliform(pink colony) are absent.Then what should be the report? Total coliform=70 CFU E coli= 70 CFU - is this to be written in the report? Please clear my doubt

@radhaanand1099 14 күн бұрын

For incubation they are showing hot air oven

@Microbiologyvala 4 ай бұрын

This video is amazing mam, but I have a question, please reply. Actually, you told that E coli will appear blue in color on this media, but it is a gram negative bacteria. Then it should appear pink in color. Please tell me why this happens.

@brianmarv Ай бұрын

Can this Buchner's funnel be cleaned with flamed methanol without being damaged or only an autoclave?

@MdSaidalSaba 8 ай бұрын

best wishes from my water chemistry lab

@KomalpreetKaur-os6pb 3 жыл бұрын

GOOD EXPLANATION:)

@MicroChemsExperiments 3 жыл бұрын

Thank you.

@dheerajtiwari920 11 ай бұрын

Very informative Video ....

@MicroChemsExperiments 11 ай бұрын

Glad you liked it

@lalitasaini544 3 жыл бұрын

Amazing experiment 🙌

@MicroChemsExperiments 3 жыл бұрын

Thank you. Keep supporting us.

@lithujoseph3474 3 жыл бұрын

Thanks for the video.. can u explain and do a video on water sampling , normally checking for water quality in Industries

@MicroChemsExperiments 3 жыл бұрын

We will make video later. Thank you

@gracefulheart7910 3 жыл бұрын

If we cannot count the number of "blue colonies", & "blue & purple colonies" we have to do dilution before filtering. So in that case, how to do dilution ? And how to do calculations ? Plzz explain. Thanks.

@MicroChemsExperiments 3 жыл бұрын

For dilution: mix 1 sample with 99ml sterile distilled water. Calculation: Number of colony × 100

@aishamuhammad9010 9 ай бұрын

Plz explain 🙏

@nadiaariba5118 Жыл бұрын

Thanks for explaining

@MicroChemsExperiments Жыл бұрын

You're welcome

@fiacreishimwe7275 2 жыл бұрын

Thank you, I understand it clearly.

@MicroChemsExperiments 2 жыл бұрын

Thanks for staying with us

@materialsandtechnology8956 Жыл бұрын

kzfaq.info/get/bejne/q92Xh5lmqs-9YYU.html watch this video of membrane for water purification

@knowledgeuptocorner5863 2 жыл бұрын

Nicely explained...

@MicroChemsExperiments 2 жыл бұрын

Thanks. Stay with us

@jandecoole5687 2 жыл бұрын

Thank you great bexplaination

@MicroChemsExperiments 2 жыл бұрын

Glad you liked it

@anaispelleteret3123 Жыл бұрын

Is it normal to observe something at the extremities of the filter ? Are they growing colonies ? Or do some metabolites reacts with the medium ? Or is it something else ?

@vaidehipatel2630 2 ай бұрын

Is for the fruit Slurry is the same.

@JayAcharya-fz4kl 7 ай бұрын

Please make a video on MPN in a similar manner

@mirnahazem3702 Ай бұрын

is it true to sterilize the filtration units in oven?

@ziangvo1877 Жыл бұрын

Thanks for the video. Can u show me details preparation for the negative and positive control plz?

@MicroChemsExperiments Жыл бұрын

Ok. We will make video later

@user-hq5zb1su7k 8 ай бұрын

Great! Thank you...But I would like to know if you worked with hydrophilic or hydrophobic membranes

@namitaarya3517 4 ай бұрын

In this viedo you have told that incubate the petri plate without inverting it ,but in ISO 9308-1 it there to invert the petri plate. Which is correct please make it sure.

@MicroChemsExperiments 4 ай бұрын

Filter paper is on the the culture media of the petri plate. So the plate should not invert

@theconqueror24568 3 жыл бұрын

Can you pls share with us the method of the most probable number technique (MPN) also?

@MicroChemsExperiments 3 жыл бұрын

Ok, we will make video on it soon. Stay with us.

@Adidthya 11 ай бұрын

Thank you dear.Can you please mention the specification of filtration unit?

@aasthaashi0500 2 жыл бұрын

Hello. Thanks for the video. It was nicely explained. I would like to ask that how will we determine the water quality using this filter membrane method and what are some disadvantages of using this method.

@MicroChemsExperiments 2 жыл бұрын

If pathogenic bacteria is found, means that the water is bad in quality. Only disadvantage is that it is applicable for water sample with low bacterial count

@aasthaashi0500 2 жыл бұрын

@@MicroChemsExperiments Thank you so much. Really appreciated.❤

@Bharatpithiyalive 2 жыл бұрын

Please suggested another techniques for purified water testing

@futurematerialsandtech5766 2 жыл бұрын

Electrospun PVDF-carbon membranes preparation for water filtration. Pls watch our video kzfaq.info/get/bejne/jNVygrCju8ioqYk.html

@materialsandtechnology8956 Жыл бұрын

kzfaq.info/get/bejne/q92Xh5lmqs-9YYU.html watch this video of membrane for water purification

@KaarlisBaumanis Жыл бұрын

Which Agar you would suggest for counting colonies at 22 and 36 degrees?

@liesdiebibelbruder420 5 ай бұрын

So how are the Membrane Filters Sterilised? They don't seem to be Sterile.

@MicroChemsExperiments 5 ай бұрын

It is sterilized by Autoclaving

@liesdiebibelbruder420 5 ай бұрын

@@MicroChemsExperiments do you put them in aluminium foil for autoclaving?

@safaafrin5391 9 ай бұрын

Please upload the video of legionella detection

@misbachufmunir87 3 ай бұрын

Thanks

@lilianas9702 Жыл бұрын

Hi, I have a question about colonies on CCA chromogenic coliforms. Can we extend the incubation? Sometimes yellow colonies appear after 24 hours, which become pink with longer incubation. In this case, are there also coliform bacteria?

@enviromantallabreport Жыл бұрын

cant you use M -Fc agar LES for E.coli and M Endo Agar LES for total coliform organisms???by seeing this i can feel that results would be very hard to take

@aishamuhammad9010 9 ай бұрын

So plz how do you calculate for CFU. If the colonies are 180

@sharyanazir9850 2 жыл бұрын

Amazing.

@MicroChemsExperiments 2 жыл бұрын

Thank you

@theophilust9736 3 жыл бұрын

Nice video ! Please can this technique be used for testing of Ecoli,Coliform,salmonella,pseudomonas and staphylococcus organisms present in sanitary pad. And if yes will it involve just diluting the sample in water ?

@MicroChemsExperiments 3 жыл бұрын

Yes. This method can be applied after diluting your sample in sterile distilled water if the expected microbial count is low. But if the expected microbial count in your sample is high, then you have to follow different method as I have already uploaded for enumeration of E.coli, coliform & salmonella from different sample. Please find those tests from my channel.

@futurematerialsandtech5766 2 жыл бұрын

Electrospun PVDF-carbon membranes preparation for water filtration. Pls watch our video kzfaq.info/get/bejne/jNVygrCju8ioqYk.html

@joshuabansah7366 2 жыл бұрын

Thanks for video

@MicroChemsExperiments 2 жыл бұрын

You are welcome

@sanjuktaghosh2886 Ай бұрын

How the colour has come in bacterial colony?

@MicroChemsExperiments Ай бұрын

Everything is described in this video. Watch the video again

@sangrammore3985 Жыл бұрын

How could you use filter paper without pre wet

@MicroChemsExperiments Жыл бұрын

Try as shown in this video. It will work

@arima6210 5 ай бұрын

What agar plate that you used to measure the e coli

@MicroChemsExperiments 5 ай бұрын

Chromogenic Coliform Agar

@keepgoing6630 2 жыл бұрын

Good introduction. Can we put the filter paper into the liquid media in the flask after filtration instead of the Petri plates for growing the bacteria?

@MicroChemsExperiments 2 жыл бұрын

Yes. You can.

@alicemori7327 Жыл бұрын

hi, i have a question. does the filtration appratus need to be autoclave for each sample? Thank you

@MicroChemsExperiments Жыл бұрын

Yes

@rejinaprajapati2571 2 жыл бұрын

you say, for the preparation of the chromocult coliform agar, we need to dissolve 5.5 g of dehydrated power of agar, but you had written 2.55 g of to dissolve in 100 ml distilled water. Can you please clear me my confusion?

@MicroChemsExperiments 2 жыл бұрын

Ops sorry, we did mistake while saying. It should be 2.55g for 100ml.

@Bhabita-k8q 2 жыл бұрын

Nice video

@MicroChemsExperiments 2 жыл бұрын

Thank you

@alicemori7327 11 ай бұрын

Hi, i have a question? How do i sterilise the agar medium since it can't be autoclaved?

@MicroChemsExperiments 10 ай бұрын

No need to sterilize it. Just dissolve the media as per the instruction mentioned in the label or catalog of the media

@panggogi348 Жыл бұрын

thank you

@MicroChemsExperiments Жыл бұрын

You are welcome

@fadilanwar.official Жыл бұрын

What is the name of this tool? Where we can buy it?

@ewelina3817 Жыл бұрын

Hey, could you tell me why you "don't invert plates during incubation". If you have some references to this method I would be appreciate if you could share. Thank you.

@imonroy1928 Жыл бұрын

Hi, I feel if we incubate it in an inverted position, the filter paper may fall off from the media side of the plate.

@MicroChemsExperiments Жыл бұрын

You shouldn't invert the plate because filter paper is on the surface of the media. Reference: Iso 9308-1:2017

@abdelrhmanali4215 7 ай бұрын

⁠⁠@@MicroChemsExperiments thanks for the useful info, but as in ISO 9308-1/2014: after filteration, place the membrane filter on the agar. ensuring that no air is trapped underneath, invert petridish and incubate at 36 *c for 24h!

@Salmankhan-rj7vs Ай бұрын

Which type filter you're use

@MicroChemsExperiments Ай бұрын

Everything is described in this video. Watch the video again

@shravanis1755 Жыл бұрын

If Colonies are growing outside the membrane that is below the membrane ,then what is the problem Mam?

@MicroChemsExperiments Жыл бұрын

It indicates cross contamination. Don't count them during result interpretation. only count colonies on the filter paper

@shravanis1755 Жыл бұрын

@@MicroChemsExperiments thank you

@kinetomagnetic Жыл бұрын

Thanks, one question: if the filter paper is not inverted while placing on agar, how the filtered microbes present at the top side of filter come in contact with the media?

@MicroChemsExperiments Жыл бұрын

Microorganisms absorb nutrients from the media through the semi permeable filter paper

@kinetomagnetic Жыл бұрын

@@MicroChemsExperiments Thanks!

@jibantaraahongshangbam157 2 жыл бұрын

Membrane filtration technique for water samples using chromocult agar I observed some transparent colonies apart from pink and blue colonies. So, I want to ask what organism is that transparent colonies. Can u explain.

@MicroChemsExperiments 2 жыл бұрын

You can use differential media and then conduct biochemical or serological experiments to confirm those colonies.

@AnyaohaMichael 6 ай бұрын

Can I use nutrient agar for this analysis?

@MicroChemsExperiments 5 ай бұрын

Yes, only if you want total plate count from your sample

@abronexportsskjain5295 3 жыл бұрын

Well explained do you have any video colony counter working

@MicroChemsExperiments 3 жыл бұрын

We dont make video on instrument. In future, we will make video on different instruments. Stay with us.

@MicroChemsExperiments 3 жыл бұрын

We used colony counter in coliform count. Take a look

@Ankitsingh-bq9bu Жыл бұрын

Thank you so much mam

@MicroChemsExperiments Жыл бұрын

Most welcome 😊

@redvelvet191 2 жыл бұрын

I have question. For membrane filtration technique, it is advisable to filter 3 different volume. Do i need to sterilised the filtration unit in between filtering 3 volume or i can filter 3 different volume using the same filtration unit?

@MicroChemsExperiments 2 жыл бұрын

Sterilisation for 3 times is best but you can sterilise only once for 3 volumes of sample as you are taking same sample.

@redvelvet191 2 жыл бұрын

@@MicroChemsExperiments thank you so much for your answer

@MicroChemsExperiments 2 жыл бұрын

@@redvelvet191 you are most welcome

@thegandeeman 3 ай бұрын

I remember when this stuff was part of my continued education studies for licensure, I'm now to screwed over and drunk to care. Maybe I need methamphetamines

@shravanis1755 Жыл бұрын

Is there any method to sterilize the membrane filters if they are not sealed?

@MicroChemsExperiments Жыл бұрын

If the filter autoclavable, then autoclave it to make it sterile

@miftaamalia4995 2 жыл бұрын

Do you know what is the composition of chromogenic coliform agar media? Could you give the tutorial how to make it?

@MicroChemsExperiments Жыл бұрын

We know the composition but making in local lab is difficult

@theophilust9736 3 жыл бұрын

Thanks for the video Can you please do for testing for presence of yeast and mold in sample using this membrane filtration technique

@MicroChemsExperiments 3 жыл бұрын

The same procedure will be followed except DRBC agar plate should be used replacing the Chromocult Coliform Agar and incubate at 25 degree Celsius for 3-5 days. Total fungal colonies on membrane filter is the total yeast & mold.

@theophilust9736 3 жыл бұрын

@@MicroChemsExperiments and preparing the yeast and mold using distilled water ! Does it involve using peptone water ?

@MicroChemsExperiments 3 жыл бұрын

@@theophilust9736 Using normal physiological saline (0.85% NaCl)

@theophilust9736 3 жыл бұрын

You guys are awesome ! Love that you are willing to help I am trying to set up a lab for sanitary pad and I need agar to buy I will be testing for the presence of ecoil&coliform, yeast&mold, salmonella,staphylococcus aureus, pseudomonas aeroginosa, total viable count. Obviously I now know using membrane filtration is easier but knowing the appropriate selective agar for each is where I need help. I know for ecoli/coliform from this video and for the fungi through your response to my question. Please list the agar for salmonella, pseudomonas euroginosa,staphylococcus aureus and total viable count . I appreciate you

@MicroChemsExperiments 3 жыл бұрын

@@theophilust9736 Buy XLD agar for Salmonella, Nalidixic Cetrimide Agar for pseudomonas aeruginosa, Plate Count Agar for Total viable count and Mannitol Salt Agar for Staphylococcus aureus.

@Spring_rolls 2 жыл бұрын

What is the active ingredient that is responsible for giving color to colonies?

@MicroChemsExperiments 2 жыл бұрын

Different media have different ingredients and give different colored colonies. Please study about media composition in Google

@fiacreishimwe7275 2 жыл бұрын

Good.

@MicroChemsExperiments 2 жыл бұрын

Thank you

@user-sn4xq9nr3r Жыл бұрын

Hiw do we incorperate enrichment of the sample into this process?

@MicroChemsExperiments Жыл бұрын

Enrichment is not needed and not possible

@mansoorahmad490 2 жыл бұрын

can we use VRBA/EMB for Colifrm/E. Coli & PDA for Yeast/Mold?

@MicroChemsExperiments 2 жыл бұрын

Yes you can

@FayazKhan-gr1mx 2 жыл бұрын

Can the liquid still go thru the filter without using a vacuum pump?

@MicroChemsExperiments 2 жыл бұрын

No. Water can't pass without using vaccume pump

@sulochanasinthanaithuli1936 Жыл бұрын

sulphate reduction anaerobic bacteria test (water) vedio upload mam plz

@MicroChemsExperiments Жыл бұрын

Noted

@krishnachaitanya414 2 жыл бұрын

Can I repeat the exact same process for Salmonella but replacing chromcult Agar with XLD and keeping all the steps same? Also how to calculate the number of colonies for it? the same way?

@MicroChemsExperiments 2 жыл бұрын

Yes you can, and the calculation will be the same.

@krishnachaitanya414 2 жыл бұрын

@@MicroChemsExperiments Thank you so much!

@krishnachaitanya414 2 жыл бұрын

i could not find XLD, so using Salmonella Shigella Agar now, hope it works in the same way.

@shahidnawaz126 2 жыл бұрын

excellent information. can you help me with CFU count? im doing testings on non sterile cotton

@MicroChemsExperiments 2 жыл бұрын

Surely I can help you. Please write in details.

@jimfanahvinpg5944 8 ай бұрын

Is it good topic for msc project?

@cryptusxx 2 жыл бұрын

Thanks for the explanation. But can this method be used for contaminated food?

@MicroChemsExperiments 2 жыл бұрын

No. This method is not for the food sample

@cryptusxx 2 жыл бұрын

@@MicroChemsExperiments i see. Can you suggest what method can be used for food sample?

@tweety6878 2 жыл бұрын

At how many count of coliform indicate water potablity?????

@MicroChemsExperiments 2 жыл бұрын

Zero coliform per 100ml drinking water

@gladwelchepkemoi6249 Жыл бұрын

Is the measuring cylinder sterile ?

@MicroChemsExperiments Жыл бұрын

Yes.

@vaishnaviraotokla8758 Жыл бұрын

Can we do different filter membrane filteration for the same solution??

@MicroChemsExperiments Жыл бұрын

You can

@chinmoymandal7858 3 жыл бұрын

Voice very sweet

@MicroChemsExperiments 3 жыл бұрын

Thanks a bunch

@mehedihasanrabbi342 3 жыл бұрын

Mention the oil content test procedure

@MicroChemsExperiments 3 жыл бұрын

Which sample?

@mehedihasanrabbi342 3 жыл бұрын

@@MicroChemsExperiments bleaching earth oil content. Vegetable oil

@MicroChemsExperiments 3 жыл бұрын

We already uploaded. Video link: kzfaq.info/get/bejne/aJqhirl90p3MmKs.html

@narendrakondamoni2246 2 жыл бұрын

what is the Water testing guideline

@MicroChemsExperiments 2 жыл бұрын

Many guidelines can be found for water testing. Like American Water Association

@khoirulrozul9240 2 жыл бұрын

Can this method be used for food samples such as sugar and others?

@MicroChemsExperiments 2 жыл бұрын

No. This method is applicable only for Water sample

@shahrilizat740 3 жыл бұрын

Can i ask you?, Which regulation that you follow?? APHA or As??

@MicroChemsExperiments 3 жыл бұрын

ISO 9308

@sathisdas5465 2 жыл бұрын

No need to autoclave the medium?

@MicroChemsExperiments 2 жыл бұрын

No. No need to autoclave

@cook.king0844 2 жыл бұрын

Nice❤❤

@MicroChemsExperiments 2 жыл бұрын

Thanks 🤗

@futurematerialsandtech5766 2 жыл бұрын

@@MicroChemsExperiments Electrospun PVDF-carbon membranes preparation for water filtration. Pls watch our video kzfaq.info/get/bejne/jNVygrCju8ioqYk.html

@muralibond1373 2 жыл бұрын

as per which standard u have performed these tests..

@MicroChemsExperiments 2 жыл бұрын

ISO

@eyadomar00 Жыл бұрын

how much do the total test parts cost?

@MicroChemsExperiments Жыл бұрын

Varies with the type and brand of the used media and equipment

@deshdeepak098 2 жыл бұрын

Mam we can use alternative M-indo broath.

@MicroChemsExperiments 2 жыл бұрын

No.

@alameenallu8639 2 жыл бұрын

Is it .22 or .45 micron filtr??

@MicroChemsExperiments 2 жыл бұрын

0.45

@qatetrapilpl3630 2 жыл бұрын

Is this filter paper sterile?

@MicroChemsExperiments 2 жыл бұрын

Yes

@admeliora6040 9 ай бұрын

Thought I was lucky to have found a technical video for exactly what I need. >Indian voice >sigh

@sandhyaranjith427 3 жыл бұрын

For heterotrophic count which culture media is used?Nutrient Agar is enough?

@MicroChemsExperiments 3 жыл бұрын

Yes. Nutrient Agar.

@sandhyaranjith427 3 жыл бұрын

@@MicroChemsExperiments Thank you. One more thing,For Fecal streptococcus and sulphite reducing bacteria which culture media is used?

@MicroChemsExperiments 3 жыл бұрын

@@sandhyaranjith427 Bile Esculin Azide Agar is used for Fecal streptococcus amd Tryptose-sulfite-cycloserine (TSC) agar is used for Sulphite Reducing Bacteria

@sandhyaranjith427 3 жыл бұрын

@@MicroChemsExperiments Thank you so much.

@MicroChemsExperiments 3 жыл бұрын

@@sandhyaranjith427 You are welcome.