Can u tell me if there is any vacancy available in Nestle?
@n4nishiiiii2 жыл бұрын
So you only perform water testing?
@muralibond13732 жыл бұрын
what's the package bro...
@me..78852 жыл бұрын
Can not get over how amaizing ur explaination is...all r grateful for that massive work 🌹🌹🌹🌹
@MicroChemsExperiments Жыл бұрын
Thanks a bunch
@ucjakozicka9786 ай бұрын
The explanation was clear, concise and informative, I learnt all I needed to for a project Im working on, thank you!
@MicroChemsExperiments6 ай бұрын
Thank you so much
@MicroChemsExperiments6 ай бұрын
Send email for get help/suggestions about your practical knowledge (mail.mic.chem@gmail.com)
@agboalfred45672 жыл бұрын
This is beautiful and so easy to comprehend. Thanks a lot
@MicroChemsExperiments2 жыл бұрын
You are welcome. Stay with us
@01kanan25 күн бұрын
Good 👍information and steps of Coliform Group E.coli found proper
@01kanan25 күн бұрын
Please mam red colonies are gram negative bacteria mostly.
@redweasleypotter11274 ай бұрын
Thank for this video. So informative and another technique is added to my list
@prafull07prajapati832 жыл бұрын
You are talented and delivers the lecture very smooth Thank you so much
@MicroChemsExperiments2 жыл бұрын
Thank you so much. Stay with us
@materialsandtechnology8956 Жыл бұрын
kzfaq.info/get/bejne/q92Xh5lmqs-9YYU.html watch this video of membrane for water purification
@vsrpinternational38635 ай бұрын
Brilliant..Continue the journey of knowledge.
@MicroChemsExperiments5 ай бұрын
Thank you, I will
@gyss3349 Жыл бұрын
Nice.i. appreciating & thanks all of you for giving me scientific knowledge regarding to microbiology & Micology in general.
@MicroChemsExperiments Жыл бұрын
Our pleasure!
@SauravKumar-hm9un3 жыл бұрын
Nice video....plz make a video on multiple fermantation tube techniques for fecal coliform .
@nisasa48143 жыл бұрын
yess agree👆
@MicroChemsExperiments3 жыл бұрын
Noted the parameter. We will work on it soon. Stay with us.
@sushantpatil667927 күн бұрын
@@MicroChemsExperimentswhen you will upload it?
@rakiborrahman136824 күн бұрын
Perfect deliberation...👍 Thank you. May I have your gram negative strain photographic analysis under Florence Microscope please Dr. R.Rehman
@cyrusngullie8678 Жыл бұрын
Very nice, I really like it. just one rectification. It says incubate the plate @35 degree but in the video it showed putting the plate in the hot air oven.
@MicroChemsExperiments Жыл бұрын
We are using the Hot Air Oven as incubator.
@arzoosundrani14153 жыл бұрын
Very nice video
@MicroChemsExperiments3 жыл бұрын
Thank you
@sghosh1234Ай бұрын
I have a query..Suppose E coli (blue colony) is present in 70 CFU and coliform(pink colony) are absent.Then what should be the report? Total coliform=70 CFU E coli= 70 CFU - is this to be written in the report? Please clear my doubt
@radhaanand109914 күн бұрын
For incubation they are showing hot air oven
@Microbiologyvala4 ай бұрын
This video is amazing mam, but I have a question, please reply. Actually, you told that E coli will appear blue in color on this media, but it is a gram negative bacteria. Then it should appear pink in color. Please tell me why this happens.
@brianmarvАй бұрын
Can this Buchner's funnel be cleaned with flamed methanol without being damaged or only an autoclave?
@MdSaidalSaba8 ай бұрын
best wishes from my water chemistry lab
@KomalpreetKaur-os6pb3 жыл бұрын
GOOD EXPLANATION:)
@MicroChemsExperiments3 жыл бұрын
Thank you.
@dheerajtiwari92011 ай бұрын
Very informative Video ....
@MicroChemsExperiments11 ай бұрын
Glad you liked it
@lalitasaini5443 жыл бұрын
Amazing experiment 🙌
@MicroChemsExperiments3 жыл бұрын
Thank you. Keep supporting us.
@lithujoseph34743 жыл бұрын
Thanks for the video.. can u explain and do a video on water sampling , normally checking for water quality in Industries
@MicroChemsExperiments3 жыл бұрын
We will make video later. Thank you
@gracefulheart79103 жыл бұрын
If we cannot count the number of "blue colonies", & "blue & purple colonies" we have to do dilution before filtering. So in that case, how to do dilution ? And how to do calculations ? Plzz explain. Thanks.
@MicroChemsExperiments3 жыл бұрын
For dilution: mix 1 sample with 99ml sterile distilled water. Calculation: Number of colony × 100
@aishamuhammad90109 ай бұрын
Plz explain 🙏
@nadiaariba5118 Жыл бұрын
Thanks for explaining
@MicroChemsExperiments Жыл бұрын
You're welcome
@fiacreishimwe72752 жыл бұрын
Thank you, I understand it clearly.
@MicroChemsExperiments2 жыл бұрын
Thanks for staying with us
@materialsandtechnology8956 Жыл бұрын
kzfaq.info/get/bejne/q92Xh5lmqs-9YYU.html watch this video of membrane for water purification
@knowledgeuptocorner58632 жыл бұрын
Nicely explained...
@MicroChemsExperiments2 жыл бұрын
Thanks. Stay with us
@jandecoole56872 жыл бұрын
Thank you great bexplaination
@MicroChemsExperiments2 жыл бұрын
Glad you liked it
@anaispelleteret3123 Жыл бұрын
Is it normal to observe something at the extremities of the filter ? Are they growing colonies ? Or do some metabolites reacts with the medium ? Or is it something else ?
@vaidehipatel26302 ай бұрын
Is for the fruit Slurry is the same.
@JayAcharya-fz4kl7 ай бұрын
Please make a video on MPN in a similar manner
@mirnahazem3702Ай бұрын
is it true to sterilize the filtration units in oven?
@ziangvo1877 Жыл бұрын
Thanks for the video. Can u show me details preparation for the negative and positive control plz?
@MicroChemsExperiments Жыл бұрын
Ok. We will make video later
@user-hq5zb1su7k8 ай бұрын
Great! Thank you...But I would like to know if you worked with hydrophilic or hydrophobic membranes
@namitaarya35174 ай бұрын
In this viedo you have told that incubate the petri plate without inverting it ,but in ISO 9308-1 it there to invert the petri plate. Which is correct please make it sure.
@MicroChemsExperiments4 ай бұрын
Filter paper is on the the culture media of the petri plate. So the plate should not invert
@theconqueror245683 жыл бұрын
Can you pls share with us the method of the most probable number technique (MPN) also?
@MicroChemsExperiments3 жыл бұрын
Ok, we will make video on it soon. Stay with us.
@Adidthya11 ай бұрын
Thank you dear.Can you please mention the specification of filtration unit?
@aasthaashi05002 жыл бұрын
Hello. Thanks for the video. It was nicely explained. I would like to ask that how will we determine the water quality using this filter membrane method and what are some disadvantages of using this method.
@MicroChemsExperiments2 жыл бұрын
If pathogenic bacteria is found, means that the water is bad in quality. Only disadvantage is that it is applicable for water sample with low bacterial count
@aasthaashi05002 жыл бұрын
@@MicroChemsExperiments Thank you so much. Really appreciated.❤
@Bharatpithiyalive2 жыл бұрын
Please suggested another techniques for purified water testing
@futurematerialsandtech57662 жыл бұрын
Electrospun PVDF-carbon membranes preparation for water filtration. Pls watch our video kzfaq.info/get/bejne/jNVygrCju8ioqYk.html
@materialsandtechnology8956 Жыл бұрын
kzfaq.info/get/bejne/q92Xh5lmqs-9YYU.html watch this video of membrane for water purification
@KaarlisBaumanis Жыл бұрын
Which Agar you would suggest for counting colonies at 22 and 36 degrees?
@liesdiebibelbruder4205 ай бұрын
So how are the Membrane Filters Sterilised? They don't seem to be Sterile.
@MicroChemsExperiments5 ай бұрын
It is sterilized by Autoclaving
@liesdiebibelbruder4205 ай бұрын
@@MicroChemsExperiments do you put them in aluminium foil for autoclaving?
@safaafrin53919 ай бұрын
Please upload the video of legionella detection
@misbachufmunir873 ай бұрын
Thanks
@lilianas9702 Жыл бұрын
Hi, I have a question about colonies on CCA chromogenic coliforms. Can we extend the incubation? Sometimes yellow colonies appear after 24 hours, which become pink with longer incubation. In this case, are there also coliform bacteria?
@enviromantallabreport Жыл бұрын
cant you use M -Fc agar LES for E.coli and M Endo Agar LES for total coliform organisms???by seeing this i can feel that results would be very hard to take
@aishamuhammad90109 ай бұрын
So plz how do you calculate for CFU. If the colonies are 180
@sharyanazir98502 жыл бұрын
Amazing.
@MicroChemsExperiments2 жыл бұрын
Thank you
@theophilust97363 жыл бұрын
Nice video ! Please can this technique be used for testing of Ecoli,Coliform,salmonella,pseudomonas and staphylococcus organisms present in sanitary pad. And if yes will it involve just diluting the sample in water ?
@MicroChemsExperiments3 жыл бұрын
Yes. This method can be applied after diluting your sample in sterile distilled water if the expected microbial count is low. But if the expected microbial count in your sample is high, then you have to follow different method as I have already uploaded for enumeration of E.coli, coliform & salmonella from different sample. Please find those tests from my channel.
@futurematerialsandtech57662 жыл бұрын
Electrospun PVDF-carbon membranes preparation for water filtration. Pls watch our video kzfaq.info/get/bejne/jNVygrCju8ioqYk.html
@joshuabansah73662 жыл бұрын
Thanks for video
@MicroChemsExperiments2 жыл бұрын
You are welcome
@sanjuktaghosh2886Ай бұрын
How the colour has come in bacterial colony?
@MicroChemsExperimentsАй бұрын
Everything is described in this video. Watch the video again
@sangrammore3985 Жыл бұрын
How could you use filter paper without pre wet
@MicroChemsExperiments Жыл бұрын
Try as shown in this video. It will work
@arima62105 ай бұрын
What agar plate that you used to measure the e coli
@MicroChemsExperiments5 ай бұрын
Chromogenic Coliform Agar
@keepgoing66302 жыл бұрын
Good introduction. Can we put the filter paper into the liquid media in the flask after filtration instead of the Petri plates for growing the bacteria?
@MicroChemsExperiments2 жыл бұрын
Yes. You can.
@alicemori7327 Жыл бұрын
hi, i have a question. does the filtration appratus need to be autoclave for each sample? Thank you
@MicroChemsExperiments Жыл бұрын
Yes
@rejinaprajapati25712 жыл бұрын
you say, for the preparation of the chromocult coliform agar, we need to dissolve 5.5 g of dehydrated power of agar, but you had written 2.55 g of to dissolve in 100 ml distilled water. Can you please clear me my confusion?
@MicroChemsExperiments2 жыл бұрын
Ops sorry, we did mistake while saying. It should be 2.55g for 100ml.
@Bhabita-k8q2 жыл бұрын
Nice video
@MicroChemsExperiments2 жыл бұрын
Thank you
@alicemori732711 ай бұрын
Hi, i have a question? How do i sterilise the agar medium since it can't be autoclaved?
@MicroChemsExperiments10 ай бұрын
No need to sterilize it. Just dissolve the media as per the instruction mentioned in the label or catalog of the media
@panggogi348 Жыл бұрын
thank you
@MicroChemsExperiments Жыл бұрын
You are welcome
@fadilanwar.official Жыл бұрын
What is the name of this tool? Where we can buy it?
@ewelina3817 Жыл бұрын
Hey, could you tell me why you "don't invert plates during incubation". If you have some references to this method I would be appreciate if you could share. Thank you.
@imonroy1928 Жыл бұрын
Hi, I feel if we incubate it in an inverted position, the filter paper may fall off from the media side of the plate.
@MicroChemsExperiments Жыл бұрын
You shouldn't invert the plate because filter paper is on the surface of the media. Reference: Iso 9308-1:2017
@abdelrhmanali42157 ай бұрын
@@MicroChemsExperiments thanks for the useful info, but as in ISO 9308-1/2014: after filteration, place the membrane filter on the agar. ensuring that no air is trapped underneath, invert petridish and incubate at 36 *c for 24h!
@Salmankhan-rj7vsАй бұрын
Which type filter you're use
@MicroChemsExperimentsАй бұрын
Everything is described in this video. Watch the video again
@shravanis1755 Жыл бұрын
If Colonies are growing outside the membrane that is below the membrane ,then what is the problem Mam?
@MicroChemsExperiments Жыл бұрын
It indicates cross contamination. Don't count them during result interpretation. only count colonies on the filter paper
@shravanis1755 Жыл бұрын
@@MicroChemsExperiments thank you
@kinetomagnetic Жыл бұрын
Thanks, one question: if the filter paper is not inverted while placing on agar, how the filtered microbes present at the top side of filter come in contact with the media?
@MicroChemsExperiments Жыл бұрын
Microorganisms absorb nutrients from the media through the semi permeable filter paper
@kinetomagnetic Жыл бұрын
@@MicroChemsExperiments Thanks!
@jibantaraahongshangbam1572 жыл бұрын
Membrane filtration technique for water samples using chromocult agar I observed some transparent colonies apart from pink and blue colonies. So, I want to ask what organism is that transparent colonies. Can u explain.
@MicroChemsExperiments2 жыл бұрын
You can use differential media and then conduct biochemical or serological experiments to confirm those colonies.
@AnyaohaMichael6 ай бұрын
Can I use nutrient agar for this analysis?
@MicroChemsExperiments5 ай бұрын
Yes, only if you want total plate count from your sample
@abronexportsskjain52953 жыл бұрын
Well explained do you have any video colony counter working
@MicroChemsExperiments3 жыл бұрын
We dont make video on instrument. In future, we will make video on different instruments. Stay with us.
@MicroChemsExperiments3 жыл бұрын
We used colony counter in coliform count. Take a look
@Ankitsingh-bq9bu Жыл бұрын
Thank you so much mam
@MicroChemsExperiments Жыл бұрын
Most welcome 😊
@redvelvet1912 жыл бұрын
I have question. For membrane filtration technique, it is advisable to filter 3 different volume. Do i need to sterilised the filtration unit in between filtering 3 volume or i can filter 3 different volume using the same filtration unit?
@MicroChemsExperiments2 жыл бұрын
Sterilisation for 3 times is best but you can sterilise only once for 3 volumes of sample as you are taking same sample.
@redvelvet1912 жыл бұрын
@@MicroChemsExperiments thank you so much for your answer
@MicroChemsExperiments2 жыл бұрын
@@redvelvet191 you are most welcome
@thegandeeman3 ай бұрын
I remember when this stuff was part of my continued education studies for licensure, I'm now to screwed over and drunk to care. Maybe I need methamphetamines
@shravanis1755 Жыл бұрын
Is there any method to sterilize the membrane filters if they are not sealed?
@MicroChemsExperiments Жыл бұрын
If the filter autoclavable, then autoclave it to make it sterile
@miftaamalia49952 жыл бұрын
Do you know what is the composition of chromogenic coliform agar media? Could you give the tutorial how to make it?
@MicroChemsExperiments Жыл бұрын
We know the composition but making in local lab is difficult
@theophilust97363 жыл бұрын
Thanks for the video Can you please do for testing for presence of yeast and mold in sample using this membrane filtration technique
@MicroChemsExperiments3 жыл бұрын
The same procedure will be followed except DRBC agar plate should be used replacing the Chromocult Coliform Agar and incubate at 25 degree Celsius for 3-5 days. Total fungal colonies on membrane filter is the total yeast & mold.
@theophilust97363 жыл бұрын
@@MicroChemsExperiments and preparing the yeast and mold using distilled water ! Does it involve using peptone water ?
@MicroChemsExperiments3 жыл бұрын
@@theophilust9736 Using normal physiological saline (0.85% NaCl)
@theophilust97363 жыл бұрын
You guys are awesome ! Love that you are willing to help I am trying to set up a lab for sanitary pad and I need agar to buy I will be testing for the presence of ecoil&coliform, yeast&mold, salmonella,staphylococcus aureus, pseudomonas aeroginosa, total viable count. Obviously I now know using membrane filtration is easier but knowing the appropriate selective agar for each is where I need help. I know for ecoli/coliform from this video and for the fungi through your response to my question. Please list the agar for salmonella, pseudomonas euroginosa,staphylococcus aureus and total viable count . I appreciate you
@MicroChemsExperiments3 жыл бұрын
@@theophilust9736 Buy XLD agar for Salmonella, Nalidixic Cetrimide Agar for pseudomonas aeruginosa, Plate Count Agar for Total viable count and Mannitol Salt Agar for Staphylococcus aureus.
@Spring_rolls2 жыл бұрын
What is the active ingredient that is responsible for giving color to colonies?
@MicroChemsExperiments2 жыл бұрын
Different media have different ingredients and give different colored colonies. Please study about media composition in Google
@fiacreishimwe72752 жыл бұрын
Good.
@MicroChemsExperiments2 жыл бұрын
Thank you
@user-sn4xq9nr3r Жыл бұрын
Hiw do we incorperate enrichment of the sample into this process?
@MicroChemsExperiments Жыл бұрын
Enrichment is not needed and not possible
@mansoorahmad4902 жыл бұрын
can we use VRBA/EMB for Colifrm/E. Coli & PDA for Yeast/Mold?
@MicroChemsExperiments2 жыл бұрын
Yes you can
@FayazKhan-gr1mx2 жыл бұрын
Can the liquid still go thru the filter without using a vacuum pump?
@MicroChemsExperiments2 жыл бұрын
No. Water can't pass without using vaccume pump
@sulochanasinthanaithuli1936 Жыл бұрын
sulphate reduction anaerobic bacteria test (water) vedio upload mam plz
@MicroChemsExperiments Жыл бұрын
Noted
@krishnachaitanya4142 жыл бұрын
Can I repeat the exact same process for Salmonella but replacing chromcult Agar with XLD and keeping all the steps same? Also how to calculate the number of colonies for it? the same way?
@MicroChemsExperiments2 жыл бұрын
Yes you can, and the calculation will be the same.
@krishnachaitanya4142 жыл бұрын
@@MicroChemsExperiments Thank you so much!
@krishnachaitanya4142 жыл бұрын
i could not find XLD, so using Salmonella Shigella Agar now, hope it works in the same way.
@shahidnawaz1262 жыл бұрын
excellent information. can you help me with CFU count? im doing testings on non sterile cotton
@MicroChemsExperiments2 жыл бұрын
Surely I can help you. Please write in details.
@jimfanahvinpg59448 ай бұрын
Is it good topic for msc project?
@cryptusxx2 жыл бұрын
Thanks for the explanation. But can this method be used for contaminated food?
@MicroChemsExperiments2 жыл бұрын
No. This method is not for the food sample
@cryptusxx2 жыл бұрын
@@MicroChemsExperiments i see. Can you suggest what method can be used for food sample?
@tweety68782 жыл бұрын
At how many count of coliform indicate water potablity?????
@MicroChemsExperiments2 жыл бұрын
Zero coliform per 100ml drinking water
@gladwelchepkemoi6249 Жыл бұрын
Is the measuring cylinder sterile ?
@MicroChemsExperiments Жыл бұрын
Yes.
@vaishnaviraotokla8758 Жыл бұрын
Can we do different filter membrane filteration for the same solution??
We already uploaded. Video link: kzfaq.info/get/bejne/aJqhirl90p3MmKs.html
@narendrakondamoni22462 жыл бұрын
what is the Water testing guideline
@MicroChemsExperiments2 жыл бұрын
Many guidelines can be found for water testing. Like American Water Association
@khoirulrozul92402 жыл бұрын
Can this method be used for food samples such as sugar and others?
@MicroChemsExperiments2 жыл бұрын
No. This method is applicable only for Water sample
@shahrilizat7403 жыл бұрын
Can i ask you?, Which regulation that you follow?? APHA or As??
@MicroChemsExperiments3 жыл бұрын
ISO 9308
@sathisdas54652 жыл бұрын
No need to autoclave the medium?
@MicroChemsExperiments2 жыл бұрын
No. No need to autoclave
@cook.king08442 жыл бұрын
Nice❤❤
@MicroChemsExperiments2 жыл бұрын
Thanks 🤗
@futurematerialsandtech57662 жыл бұрын
@@MicroChemsExperiments Electrospun PVDF-carbon membranes preparation for water filtration. Pls watch our video kzfaq.info/get/bejne/jNVygrCju8ioqYk.html
@muralibond13732 жыл бұрын
as per which standard u have performed these tests..
@MicroChemsExperiments2 жыл бұрын
ISO
@eyadomar00 Жыл бұрын
how much do the total test parts cost?
@MicroChemsExperiments Жыл бұрын
Varies with the type and brand of the used media and equipment
@deshdeepak0982 жыл бұрын
Mam we can use alternative M-indo broath.
@MicroChemsExperiments2 жыл бұрын
No.
@alameenallu86392 жыл бұрын
Is it .22 or .45 micron filtr??
@MicroChemsExperiments2 жыл бұрын
0.45
@qatetrapilpl36302 жыл бұрын
Is this filter paper sterile?
@MicroChemsExperiments2 жыл бұрын
Yes
@admeliora60409 ай бұрын
Thought I was lucky to have found a technical video for exactly what I need. >Indian voice >sigh
@sandhyaranjith4273 жыл бұрын
For heterotrophic count which culture media is used?Nutrient Agar is enough?
@MicroChemsExperiments3 жыл бұрын
Yes. Nutrient Agar.
@sandhyaranjith4273 жыл бұрын
@@MicroChemsExperiments Thank you. One more thing,For Fecal streptococcus and sulphite reducing bacteria which culture media is used?
@MicroChemsExperiments3 жыл бұрын
@@sandhyaranjith427 Bile Esculin Azide Agar is used for Fecal streptococcus amd Tryptose-sulfite-cycloserine (TSC) agar is used for Sulphite Reducing Bacteria