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i guess im asking the wrong place but does anyone know a method to log back into an instagram account..? I somehow lost my account password. I love any help you can offer me!

@Decker Jadiel instablaster ;)

Hi Cristina, The outcome that you describe indicates insufficient differentiation in acid alcohol following application of Hx. I would suggest using fresh acid alcohol or increasing your time in acid alcohol. To be sure, you should examine your slide by microscope following each round of differentiation and subsequent blueing. Hope this helps. Let me know how it goes! Bye for now Damien

Good question. While the basic morphological characteristics of different leukocytes can often be seen quite well in H&E stained sections (e.g., polymorphonuclear leukocytes versus monocytes and lymphocytes), immunohistochemistry enables specific subsets of leukocytes to be demonstrated (e.g., CD4 versus CD8 positive lymphocytes).

Hi Sana, I’m not a pathologist, but can provide advice on histological staining techniques to client level members of my KZfaq channel.

Yes. Just stain more with Hx.

Hi Ravi, we use a number of different hematoxylin recipes but mainly use Ehrlich's when performing H&E stains. You can buy from a commercial supplier but we prepare as follows. Haematoxylin: 16 g Absolute Ethanol: 480 mL Aluminium Potassium Sulphate: 48 g De-ionised water: 240 mL Glycerol: 240 mL Glacial Acetic Acid: 24 mL Dissolve the haematoxylin in alcohol using gentle heat. Dissolve the Aluminium Potassium Sulphate in water in the same way and whilst still warm add the glycerol. Allow to cool. Add the haematoxylin solution in small portions and mix well. Finally add the acetic acid. Plug the container with cotton wool or other loose stopper and allow to ripen by exposure to light, this will take 4 6 weeks but may be allowed to continue indefinitely. Filter before use (e.g. Whatman No. 1 filter paper). If necessary, more immediate oxidation can be achieved by adding 0.1g of Sodium Iodate per 100 mL of stain. Allow to stand for 1hr prior to use. Our eosin solution is prepared by combining the following but can also be purchased commercially. Absolute Alcohol: 444ml De-ionised water: 24ml 1% aqueous Eosin Y: 60ml 1% aqueous Phloxine: 1.5ml Glacial Acetic Acid: 2.4ml Good luck with your staining! Damien

@@damienharkin Thank you so much sir.....

Brisbane, Australia.

Hello. 15 minutes should certainly be long enough to see some staining with Hx. Are you preparing the Hx yourself or buying from a supplier. Assuming that the Hx Solution is fine, it is possible to remove if treat for too long in acid alcohol (ie. if using a regressive staining technique), but you don’t make mention of using this. Another possibility is that slides have not been dewaxed sufficiently to allow entry of stains, but this seems unlikely given your time in xylene. Does the eosin stain the tissue at all?

Another possibility is that you are heating your slides at 90 degrees rather than 60-70 degrees C. This should be ok, but just something to consider in case causing some damage to sections. I also assume that the tissue has been initially fixed and processed correctly.