Microscopy: Deconvolution Microscopy (David Agard)

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Күн бұрын

www.ibiology.org/talks/deconv...
Deconvolution is a technique to calculate a model for the object that gave rise to the microscope image using knowledge about the way the microscope forms images. This lecture describes the basic principles of various deconvolution techniques and introduces principles important to deconvolution such as the Fourier transform, points spread function and optical transfer function.

Пікірлер: 22

@zhenhongdu 4 жыл бұрын

I took about 3 hours to carefully listen to this great talk!Maybe this is what the master like!

@liangwenxuan 2 жыл бұрын

The correct grammar is: it took me 3 hours to ...

@andreamckerry2961 2 жыл бұрын

This is such a great video. Not only does it tell you what it is but gives a good scope of why it is useful and applications to use it for.

@pianofantast91 4 жыл бұрын

You are the best teacher ever.

@NguyetMinhTDAO 8 жыл бұрын

Thank you so much for this video!

@SahinKupusoglu 7 жыл бұрын

Great video, thank you...

@leighstanger8988 4 жыл бұрын

great video thank you. I wish i had seen it before i stared writing my thesis like my ideas were not already well known.

@moniii_666 3 жыл бұрын

This is sooooooooo helpful. Thank you

@user-eu5lw5rs3p 7 жыл бұрын

Very useful, thanks a lot

@JulioSSierraCamarena Жыл бұрын

Nice explanation, clear and detailed.

@stevengill1736 2 жыл бұрын

Deconvolution - great name for a punk band! ;*] This reminds me of how telescopes correct astronomical images - neat!

@yn8093 3 жыл бұрын

Thank you for this video. 🙆

@lingyunwang3951 2 жыл бұрын

It's so good, and it can help me learn more about PSF. Actually, i do some work about it.

@davidskeet6220 3 жыл бұрын

Thank you for describing this very powerful technique. I am an amateur microscopist and would like to try the simplest 3-stack, "nearest neighbour" deconvolution technique using Matlab on acquired images. But obviously, before I start, I need to acquire the PSF of my optics. You didn't mention how PSF's are acquired. Is there a special test slide that can be purchased or constructed? Presumably it would be a grid of small apertures in an opaque thin material?

@lavandeneth9965 3 жыл бұрын

watching a master level Video from undergraduate level ;)

@blanca4287 9 жыл бұрын

Could you explain the difference between deconvolution and extended depth of focus? Thank you.

@memyselfpearl 5 жыл бұрын

Using deconvolution, you remove the blur computationally with the help of PSF and thereby improve the spatial resolution. This can also be applied for a 3D stack. In the extended depth of focus, your images are still blurred and the signal to noise ration could be still sub-optimal. Hope this helps.

@cryora 5 жыл бұрын

@@memyselfpearl What is extended depth of focus?

@shirazkaderuppan3279 4 жыл бұрын

This is a good question. To answer your question, in deconvolution microscopy, we seek to obtain spatial information which has been obscured due to the haze/blurring distortions introduced by the optics of the microscope (termed the PSF). So by means of deconvolution, you gain additional insights from the raw image, as you can now resolve additional detail within it (which cannot be obtained directly from the raw image). In extended depth of focus/field (or EDF), we simply combine different in-focus regions of an image (acquired at different image planes/sections) to generate an overall in-focus (HDR) image. No additional information may be gleaned by EDF, as the distortion introduced by the PSF of the optical system is still present (an in-focus region is still subjected to optical distortions introduced by the PSF of the system).