Monoclonal Antibody Production

Рет қаралды 92,870

10 жыл бұрын

This is a brief overview of the production of monoclonal antibodies (mAb), also known as hybridoma technology. Design inspired by Salman Khan. Special thanks to Professor DeMasi @ MCPHS University.
Davidson College's website on mAbs:
www.bio.davidson.edu/molecular...
Diagram of mAb production from the National Academies Press:
www.nap.edu/openbook.php?recor...
Software: SmoothDraw 4, CamStudio, and Premiere Pro CS6
Hardware: Bamboo CTH-460, Logitech microphone

Пікірлер: 34

@benvogel51 7 жыл бұрын

This was literally exactly what I was looking for. Succinct and informative. Thank you!

@elisemccarthy5582 7 жыл бұрын

Fantastic description and images. Really well synchronised and understandable.

@tenochtitilian 8 жыл бұрын

Wow, I knew the dumbed down explanation - but didn't realise the detailed process was so complicated. Thanks

@troyna77 3 жыл бұрын

your video deserves much more exposure. this was very informative. thank you!!!

@Wlucey 6 жыл бұрын

This is such a good explanation! Thank you!

@kCyAng93 7 жыл бұрын

Thanks so much, lifesaver! You summarized my MANY lectures in < 5 minutes (was watching @ 1.5x). :D

@inhibited44 10 жыл бұрын

Thanks again for explaining the detail of stuff . It's interesting to learn the material or at least become familiar with it.

@Queenmania2007 10 жыл бұрын

Great video, clearly explained!

@drnyashagogoma5368 9 жыл бұрын

What a fantastic explanation!! :)

@Wandelaarke 9 жыл бұрын

Perfect video! Thanks!

@TheFreeForever 10 жыл бұрын

This was a fantastic vid!!

@VictoriaGarcia-ql5kc 10 жыл бұрын

thanks for the explanation!! i can't wait for your next videos! :)

@meagharamanakumar6608 8 жыл бұрын

thank you john. you made it crystal clear :) :) :) :)

@wissutachanprasert8577 9 жыл бұрын

This is so cool thanks a lot Really help me here

@Kis4KITTY 6 жыл бұрын

great video! very interesting

@Ns205 9 жыл бұрын

U DA MAN JOHN U DA MAN

@ErikThureson 6 жыл бұрын

Well done!

@mimib1834 9 жыл бұрын

thanks so much you're the best

@dabo9181 8 жыл бұрын

Can I use CD1 mice instead of Bulb/C for Mab preparation?

@jerrylumumba7975 7 жыл бұрын

Would you be able to give like what the 4 main steps are in making these antibodies

@yaaami90 10 жыл бұрын

I don't get the step at the end where you rescreen for the specific antigen? You have immunized the mouse with one antigen in the beginning? Or do you obtain multiple B-cells with different antibodies?

@hudafaisal3860 7 жыл бұрын

you are awesome!

@gimmichhetry7082 7 жыл бұрын

sir can u plz tell me that the antigen we inject in the mouse is having single epitope or different epitopes

@thanthtooaung2979 8 жыл бұрын

thanks too :-)

@EugeneHuber 9 жыл бұрын

Great video. Could you please do one specifically for humanized MAbs?

@MariamKamel3 10 жыл бұрын

u're a genius!

@xinliu1337 7 жыл бұрын

Quick question, the hybridoma should have 4N chromosomes (2N from normal, 2N from tumor ), when these hybridoma mitosis, what will happen? Its progenitors will have 2N chromosomes? If so, if its progenitor split again, will it still have the ancestor all the hybridoma characteristics?

@nunzinho91 7 жыл бұрын

Good video ;)

@j2zel 5 жыл бұрын

Anyone know if the myeloma cells are also derived from mice? Since the activated B cells are mouse-derived, I'd imagine that the myeloma cells should also be in order for fusion to occur.

@greenroofmobilhomes4653 7 жыл бұрын

bác Sỹ John giỏi quá

@Ns205 9 жыл бұрын

FREAKING HAAAAGOP

@alessiamartina 7 жыл бұрын

He didn't say that the first animal dies, that's one of the reasons why pAb are more widely used, and it explains why only mouse and rabbit mAb exist. For ethical reasons, they wouldn't sacrifice a goat to take its spleen. This is what a professor told me.

@farhanaanni910 6 жыл бұрын

U should have talk slower so that every single student from different countries also get these... and i think u should have talk lesser and write more so that the advantages and disadvantages which u were describing could be visualized by the reader...i think that would help me more