mRNA Manufacturing

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mRNA Manufacturing

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Күн бұрын

mRNA Manufacturing - Anton McCaffrey
Education Session from the American Society of Gene & Cell Therapy's 22nd Annual Meeting

Пікірлер: 81

@barbarab202 3 жыл бұрын

So how do researchers develop these mRNA vaccines? Is the mRNA visible under electron or atomic microscopes? How do you work / replace messenger pieces at this minute scale?

@AA-gl1dr 3 жыл бұрын

Great question! They verify sequences using nucleotide sequencing. Look up polymerase chain reaction for a good introduction. Then look up Sanger termination sequencing and then illumina- sequencing by synthesis, then pacific biosciences SMRT and Oxford nanopore. that’ll give you a good idea of how it’s being measured. Gel electrophoresis and Sanger sequencing are the oldest methods and the slowest but one of the easiest to understand. I recommend learning about polymerase chain reaction at the same time because it’s required for all but the most recent sequencing methods.

@Tofu.87 2 жыл бұрын

What is the source material for the mRNA? What is it made of and where did it come from? Is it from an animal and if so which one?

@lidarman2 2 жыл бұрын

Wow this was uploaded a month before this technology would be needed writ large.

@cynthiagonzalez658 2 жыл бұрын

You don't work these molecules by sight. You know it's working based on indicators/labels.

@3vilJOHN Жыл бұрын

Ps the catalyst is Gold, you need a lot of it after retiring from being a donor. I found a lot of Gold. The stoichiometry for gold extraction is ore specific and you have to make a formula to get it each time. It's amazing seeing what you can do with stoichiometry and the chemists values getting it balanced the right way. In wet chemistry I can get several ounces waking something up that's never been awake before. The telluride is totally Nuclear Dude!

@therealrockguy100 3 жыл бұрын

I've never felt so ignorant after watching something. I have a lot of reading to do. I wanted to know the process of making mrna from start to finish. I need to know a lot more before I get to this point. Can you suggest any videos or books to start with? Thanks.

@Tubecraft1 2 жыл бұрын

Same here and makes you wonder at the complexity of it all. So much room for error

@margueriteoreilly2168 Жыл бұрын

Is it Graphine as the plastic Lipids

@ladyalexander2003 6 ай бұрын

Exactly! Plasmids mRNaa from bacteria do not belong in the human body and will illicit an immune response they falsely claim that’s immunity and it is not!!

@tomlee6263 3 жыл бұрын

The immune system is so incredibly complex, it's amazing!

@cynthiagonzalez658 2 жыл бұрын

@Skippy I took up microbial genetics instead, because immunology is too complicated & uncontrolled. LOL!!!

@ladyalexander2003 8 ай бұрын

Yes it is it has an immune response to any chemicals or toxins doesn’t it! They’re so a big difference between immune response and immunity! Are plasmids mRNA meant to go in the human Body ? Isn’t that a bacteria that’ll cause huge reactions in the human body and who so many people got sick when injected with this toxic concoction?

@TheEmptyeye 3 жыл бұрын

4/22/21 from cdc website.. “ Over 211 million doses of COVID-19 vaccines were administered in the United States from December 14, 2020, through April 19, 2021. During this time, VAERS received 3,486 reports of death (0.0016%) among people who received a COVID-19 vaccine.”

@shiyaoli2370 3 жыл бұрын

Where can I find the presetation slides? In the video the speaker mentioned the slides are on the website..which website?

@mourifat8659 3 жыл бұрын

I also need these presentations

@darlas5762 2 жыл бұрын

Maybe I missed it but where do they get the rna to start with? Do they make it in the lab? What is it made of?

@ladyalexander2003 3 ай бұрын

Ecoli bacteria! They took mRNA from plasmids they extracted out of bacteria! Omg the sheer stupidity! Also research chemical toxicity symptoms in humans & what causes prions & how they humanise mice! They’re literally animalising humans & causing many horrific diseases in people & half the medical community was baffled!??? 🤷‍♀️

@pkpledn6 2 жыл бұрын

How long does it take to manufacture and distribute?

@jeriahlee5335 3 жыл бұрын

Sorry I still do not know how you develop mRNA. Do you use chemical or take a cell from somewhere or from someone ? Please start from the beginning eg like baking a cake, flour, sugar etc... Do you take anything from a bat?

@JohnAbrahamsen 3 жыл бұрын

Yes. Taken from bats, from the chinese wet market.

@CharlieMacklin1 3 жыл бұрын

Jeriah Lee good question! I have been wondering the same thing myself and no one seems to have the answer. WHAT is the mRNA vaccine and HOW is it made? So far what I’ve found is that it essentially contains a pH balanced saline solution, “lipid nanoparticles” (which is basically some type of oil that can contain the mRNA) and then within each nanoparticle, mRNA. RNA is a complex molecule but basically it is going to consist of phosphorus, oxygen, carbon, hydrogen, and nitrogen. But the question is HOW is it created? And borrowed from what source? I want to know the prima materia, the starting “ingredients” for the cake they are baking. It’s not much to ask if you think about it, since it goes in the blood and all.

@peacanpie8291 3 жыл бұрын

This is also why I came to this video.

@CharlieMacklin1 2 жыл бұрын

Also, they aren’t telling people what preservatives are in it. I had a pretty bad reaction to the J&J shot from 0-20 minutes, they don’t tell you it has polysorbate 80 in it, and the other to have polyethylene glycol. These could cause allergic shock reactions. That being said I also lost an unvaccinated friend to Covid just 2 days ago, so, I really feel like we are between a rock and a hard place the way things are turning out.

@peacanpie8291 2 жыл бұрын

I asked my dr if he knew how the synthetic mRNA is made etc, and he said “that’s a good question”. He said it’s a “proprietary” thing which means they don’t divulge this info. Then I asked if he knew if each of the different brands use the same methods/ingredients for their products. He said “I don’t know but that’s another good question”. That was all I got.

@3vilJOHN Жыл бұрын

I know it's perfect timing but it was 30 years in planning. The cryo storage expires after 30 years, and the product is recommended to be used in 10 years. There were millions of quarts in storage and we had to prevent billions of dollars in collections wasted. The reserves are extremely important to our covenants nation security. And to recognize the donors have a life span donating. I maxed out donating at 450 liters and my immune wouldn't support being a donor, causing seizures, having died and cpac back to life. I didn't feel a thing dying, I woke up not breathing, the emergency injected me with lorazepam after a quick lab test to make sure the beta wouldn't cross react. After that a Donor is done. When you can collect, how many volunteers, will they return. The saline used to not taste like petroleum before the shortage. The needles used to not have nickle "ultra pure stainless only". If you can get it right, maybe you get those donors to return once like me. Main thing don't waste the stock. PS those vaccines feel like taking ectasy!

@juno6 Жыл бұрын

Poison.

@seriesbaba182 3 жыл бұрын

Can i get this slide

@arielvalverde7468 3 жыл бұрын

If another RNA Virus is present at the Moment of vaccination, Could be possible that mRNA insert in Virus modifying it instead of the target cell ?

@tomlee6263 3 жыл бұрын

I didn't think that viruses target each other, they don't canabalize each other

@ladyalexander2003 11 ай бұрын

Which species Messenger RNA is this from and what’s the danger of sick animals mRNA controlling human cells? Oh yes zoonotic disease & sickness!

@chrismunns3480 3 жыл бұрын

Can I continue my methelene blue after my vaccine? I take for brain fog. Thank you.

@AA-gl1dr 3 жыл бұрын

You should consult your physician and not the KZfaq comments.

@Hambxne 3 жыл бұрын

omg i love the internet

@mironyuk 4 жыл бұрын

Does Mrna vaccine permanently alter the genome in vivo, in the animal receiving the vacine?? Anyone know, which method is used to genetically modify a subject

@esotericyetti327 4 жыл бұрын

No, it does not alter the genetic information of cells.

@allseeinglies7091 4 жыл бұрын

Esoteric Yetti umm it absolutely does..

@armandoes 4 жыл бұрын

it is mRNA, not DNA... it shouldnt alter the genome unless there is reverse transcriptase involved

@armandoes 4 жыл бұрын

2:15 is your answer

@skinnygumbo2700 4 жыл бұрын

@CaliN8tv Sd mRNA does NOT go into nucleus where the host cell DNA resides so it's impossible to be integrated into DNA. Not to mention that a single stranded mRNA can't insert into a double stranded DNA. It needs a reverse transcriptase (RT) that would copy the information from a mRNA to a piece of DNA and then that piece of DNA would eventually be integrated in the host DNA. HIV, a RNA virus, does this, but is supplying its own RT. Human cells don't produce RT. So there are two reasons why mRNA is safe.

@harryfire411 3 жыл бұрын

self replicating RNA what will they think of next and this stuff is in my vaccine?

@alainportant6412 3 жыл бұрын

Yeah it does not sound like something worth trying.

@Tubecraft1 2 жыл бұрын

From what I see the mRNA tells the cell to make spike protein and is then finished its work I don’t think it makes more mRNA. But what concerns me is the highly complex process to make the mRNA in the first place. How do they actually know what they are putting in you. Seems like one HUGE volunteer clinical trial to me. How can they possibly know at a nano scale what’s going on

@alainportant6412 2 жыл бұрын

@@Tubecraft1 YOU shouldn't try this thing over a flu that spares 99.7% of whoever gets it. It is NOT worth fucking up your natural immune system because they WILL fuck it up and make you dependent on yearly shots to substitute what they fucked up. Or else, get ready for the Netflix monthly subscription for shots

@cynthiagonzalez658 2 жыл бұрын

@@Tubecraft1 DNA &RNA technologies have been around for decades. It's not that complicated if you know your procedures. I hopped from being a third world animal level veterinary practitioner to getting a Masters in molecular (bacterial) recombinant technology in Canada in 2 &1/2 years.

@lalamanana1912 2 жыл бұрын

@@alainportant6412 There's a big difference between 3% and 0.3%, especially given what makes Covid dangerous is not its mortality rate, but its mode of infection. That's why understanding math is so important, 3% of a billion are far(!) more deaths than 90% of ten people or so.