I cried when the intron faded away at the end

I learned more from that 2 minute video, than the 100 min lecture that my professor gave.

How many of you just went "OHHHHHH" after watching this? i know i did.

U1 binds to 5' splice site, U2 binds to the A branch. After they've been rearranged U1 and U4 dissociate. U2 and U6 have the catalytic function with regards to the transesterification reactions.

what they dont tell you is that this is all happening at the same time of transcription... the 5 cap and the polyadenylation. 10/10 animation for beginners.

Both of those terms refer to the nucleic acids (or nucleotides) that make up a strand of mRNA. The "A" refers to adenosine, and "Pyr-rich" refers to an area with a large quantity of pyrimidines. They act as signals/locations for parts of the process.

Thanks for posting these vids! I've already watched 3 and can't stop!

this is such a wonderful video! not only did it help me immediately understand the process but also made me super amazed by it! thank you!

I can't tell how helpful it was ✨ thanks a ton ❤️

I'm learning this stuff for the first time, but it's so damn interesting.

"Greatest animations of all time were all done 12, 13, 14 years ago"

splicesome are so kind.

The 5`-End (GU) of the Intron is fixing at the Branch Point (A). Introns (INTervening RegiONS) are not coded Pieces of a RNA. At the Splicing, they will be depolymerised. Exons (EXpressed regiONS) are the coded Pieces of the RNA, at the Splicing the Exon Parts will be fixed together. prä-mRNA -> (Splicing) -> mature mRNA There are also special Introns, that go in a enzymatic-conformation after Splicing. Only Eukaryotes have Introns and Exons

Too bad you didn't add the names of the different parts of the spliceosome

These videos are brilliant!

Rest in peace, intron🕊️🌹

The best ever (?) animation on RNA splicing

Still holds up, great quick video to show what's going on. Thanks!

this was so helpful, thank you very much

No way how smooth can this be omg wow

Great for showing the bigger picture, thank you.

@ilikecandycane It is a process that produces alternate mature mRNA's, in which some exons are actually removed along with introns. This leads to a large diversity of gene products from a single sequence. Usually, you would have binding inhibitors or even promoters attach to an end of an exon (splicing sequence) to either inhibit or promote splicing at that end (affinity to spliceosome). Check out the regulation of alternative splicing in the determination of sex of drosophila for an example.

u r great. Would of been helpful if u included how the extron can also be furtherly spliced

Thank you very much for the nice animation. Very clear. Congratulation.

The U subunits ARE there. They make up the spliceosome. In fact, the U1 and U4 subunits are supposed to dissociate before the lariat is formed. Of course, they didn't mention that, BUT they did show the subunit corresponding to U1 dissociate from the mRNA.

This video is very useful. Thanks a lot

Thank you for catching that--it's fixed!

What a great video!

This really helped me a lot in my medical studies! Thanks! =D

This is beautiful thanks so much

Wonderful video, thanks. I help me in me molecular biology class

This is majestic.

I SOOO AGREEE WITH YOU!!!! IT'S SO EASY TO STUDY NOW WITH ALL THESE FREAKING VIDEOS.

how awesome... school needs to adapt and include links to videos for at home, then the classroom opens up for discussion

Just amazing!!

I kinda like the weird/creepy music, lol. very very helpful, thanks.

hey thanx... nw i actually understood the actual process. it is so helpful.. this process i couldnt understood in todays class.. i do undestand nw....... thank u thank u so much. and plz............ do upload more such helpful video........:-)

Simply Brilliant

It's not correct right? Not all snRNP's stay with the spliceosome complex the whole proces, U1 and U4 should leave. Or am I wrong?

Pretty sure I didn't need to know that for my exam, but that was cooooooool!!

@soccerchic315 alternative splicing. different exon combinations will form different proteins from the same pre-mRNA

just what i needed, thanx!!

Thank you :)

I agree I learn more from you tube than I do @ florida College of Natural Health

@soccerchic315 through the process of alternative splicing. a single primary transcript can be spliced into different mRNAs by the inclusion of different sets of exons.

that is so beautiful.

Fascinating

amazing!!

GOOD INITIAL STEP TO UNDERSTAND SPLICING

thanks a million! :D

It's the description that's wrong. The video is accurate.

LOL i'm glad because im in your position now...except im nearly at the end of revision and still have few weeks left before exam :D. so boys and girls! do not start to study a day or 2 days before exam

hi I was hoping you could help me, how does alternative splicing leads to a one amino acid change in protein sequence on the same gene, with all the exons being transcribed.

video posted 15 years ago and here I am with a test on Friday lol

cristian1092 they said in the video that it will be degraded 2:16 " the introns debranched, and is then degraded"

U1 binds to 5' then u2 binds to the A branch site , then the 5' binds to the A site with help of U1 and U4 and u6 after the binding u1 and u4 leave , then u5 binds to the 3' end and cuts it , then the exons binds and that's how it's done

This is a great video! It would only be better if you could name the snRNPs as they attach to the mRNA. Also, one inaccuracy is that the video doesn't show U1 and U4 leaving the complex after the spliceosome is activated for the following transesterifications.

This video is very helpful. I love biochemistry! Woohooo!

very cool. thank you

Splicing occurs in the nucleus so that then mRNA only has exons when it leaves the nucleus to go get translated and what not

wonderful!!!

Amazing❤️

its very clear thanks a lot

pff, the music is the best part

Lovely!

Yeah, that is what happens. And I don't know why people are getting thumbs down for pointing that out because it is true. I will likely get thumbs down too for saying that. In order for the lariat to be formed...U1 and U2 MUST leave the spliceosome.

this video helpd a lot......

Hmmm... This isn't what I'm learning in my genetics class. We're being tuaght that 2'OH on the A site, with the help of splisomes that change the conformation of the mRNA, attacks the phosphodiester bond at the where the first exon and intron meet making the lariet. Next, again via conformation, the exposed 3'OH of the first exon attacks the phosphodiester bond at the remaining exon/intron connection. I guess I'll just go by what we learned in class.

VERY helpful.. thanks

thanks........ but a A phosphodiester bond is actually formed between the 4' OH and the lactase phosphofructokinase end of the ligase ester. NT

i cried that was beautiful *snif*

Two transesterifications, between GU and A, and the cleaved 3' end of the exon and the AG.

Dhanyawad (Thank You)

thank my brother

i wish my teacher knew how to teach like you

thanks!

amazing

@ChickenWingChampion I guess not proteosome, since that's for protein... Most probably, RNAse enzyme is the one degrading the introns

I'm sorry, what I wrote there is actually wrong. It's not removing exons, it's selection for them. Introns are the non-coding parts. Extrons code for the production of proteins. Why don't you just try the wikipedia articles on introns, exons, transcription and translation?

No phosphodiester bond brakes during this... just transferring of bond takes place between exons unit.

inside the nucleus, only when RNA is mature it's able to get out of the nucleus

Una figata assurda :O

Thanks

nvm...this was intended for the transcription video

Is every time a little molecule "hits" a subunit an ATP hydrolysis???

I'm looking for the 3' of the splice leader but can't identify the sequence. Any suggestions?

thanks

There is a small problem here - the pre-mRNA is not cleaved. Chemically, splicing involves two phosphotransfer reactions, not cleavage followed by ligation as this video shows.

good video, it would have been better if you would have had the names of the parts of the spliceosome

I stand corrected, "transesterification" is more accurate and precise.

I wonder how exactly they visualised this or came up with the order of work of these proteins.

thanxxx

my final is tomorrow and am reading the comments, not even watching the video, "gangsta face" how sad!

better than my so called teacher could ever do.

i am now in year ten learning this, i cant believe this video is older than me

fantastik mekanizma!

Thanks but I have a question that why eukaryotes bears both exons and introns and not prokaryotes ???

is the purpose of this DNA compression? (encoding multiple proteins) I half expect to find a version of ZLib running in molecular clockwork..

Totally just said that in a Russian accent and it was amazing.

@betteronblondes LMAO! Excellent reply.