Mycology Lids Explained: Full breakdown of liquid culture lids and back engineering media extractor

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Fresh from the Farm Fungi

Fresh from the Farm Fungi

Күн бұрын

This video breaks down the different mycology lids available for liquid culture and spore solutions. ENJOY AND MUSHLOVE
Mycology Lids Parts and accessories: www.amazon.com/shop/influence...
Mycology Master Lids with Tubing: mycologylids.com
Mycology Lids with heavy duty injection ports: lookingglassmycology.com
OG media extraction video: www.google.com/url?sa=t&rct=j...
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Пікірлер: 98
@arcies9286
@arcies9286 Жыл бұрын
People who donate experience and information are just as generous as those who donate to charity or the needy. This is one of the mushroom cultivation "treasure channels" that gives much and demands nothing but the time spent learning in return. Thank you for this and every other video you've shared inspiring aspiring mycologists o/
@mycologylids
@mycologylids Жыл бұрын
Very informative and detailed breakdown. Your passion for this subject is really evident in your explanations. I appreciate how you took the time to walk us through the different types of lids and their respective benefits. Mush love! EDIT: The tubing inner diameter is 1/4" and the outer diameter is 3/8" (for the master LC lids)
@BarackObamaJedi
@BarackObamaJedi Жыл бұрын
I made diy lids with silicone and micropore tape, works wonders, but i never thought about how the hole is needed for air displacement. Great insight!
@user-bf2zr9jk4w
@user-bf2zr9jk4w 5 ай бұрын
at first i wasn't sure about your content but i'm sold, every time i have a weird question your videos pop up with the answer...you rock 😁
@chicofromph33nix64
@chicofromph33nix64 3 ай бұрын
Same... I was searching everywhere for the purpose of the syringe filters and nobody talked about it. So I would ask in the comments and nobody would give an explanation. Found this channel and this guy has already answered 3 of my questions in 2 videos.
@DaxPegels
@DaxPegels Жыл бұрын
Yes! I have been waiting for this video. Been working on more LC and wanted to make an extractor lid. I can source all the lab stuff pretty easily
@leonardopena9237
@leonardopena9237 Жыл бұрын
Dude your videos are amazing, have learn a lot, I miss your content.
@lachyj8344
@lachyj8344 Жыл бұрын
Thanks for the video Gary! Been meaning to modify lids for LC etc for a while, so this is a calling! Mush love from Australia
@Jack-pi2lz
@Jack-pi2lz Жыл бұрын
Great stuff. Thanks for the breakdown Gary
@lornemosley8892
@lornemosley8892 Жыл бұрын
Loved the video! This will help step up my liquid cultures. Thank you so much for all the content, it's become my after work ritual to learn from your videos.
@FreshfromtheFarmFungi
@FreshfromtheFarmFungi Жыл бұрын
thanks for watching and following along!
@natishamullis-brooks5715
@natishamullis-brooks5715 7 ай бұрын
Great video! That was a small mouth quart jar, in case anyone wants to know.
@SPOREdotSTORE
@SPOREdotSTORE 4 ай бұрын
Awesome!
@MJ-bg8gn
@MJ-bg8gn 5 ай бұрын
Very good!
@jbooger13
@jbooger13 Жыл бұрын
How do you keep the end of the piping sterile? Especially if you cap it off with a syringe or leur lock cap wouldn't any lc remain in the end section? Wouldn't it be preferrable for it to run back into the jar? Thanks.
@ScottWConvid19
@ScottWConvid19 Жыл бұрын
Excellent content, as usual. Have you got an update on the morcella garden? Also, which glass pint or quart jars work best with the magnetic stirrer? I bought a case from my local garden center and they have a fairly exaggerated concaved bottom which sucks because I basically used all my culture on them to learn that the magnetic stirrer will not work with them
@FreshfromtheFarmFungi
@FreshfromtheFarmFungi Жыл бұрын
nothing popped yet 😬 it’s a half pint widemouth ball jar try getting a smaller stir bar that can help too
@archiewebster5034
@archiewebster5034 Жыл бұрын
Great video, really helpfull
@FrankEmmanuel799
@FrankEmmanuel799 Жыл бұрын
I bought some growing kits, spore prints, spawns, liquid culture and psychedelic products from an online supplier and they are affordable and discreetly delivered
@FrankEmmanuel799
@FrankEmmanuel799 Жыл бұрын
FROM TRIPWITH_JEFF
@FrankEmmanuel799
@FrankEmmanuel799 Жыл бұрын
in instagram
@nicolemichele-darkwoodland2136
@nicolemichele-darkwoodland2136 9 ай бұрын
which way do you have the filter facing in the lids and why that way?
@nickdeborbon4947
@nickdeborbon4947 5 ай бұрын
I make my own. And for my lids. One 5/32 and one 7/16 drill bits are perfect sizes for my filter and injection ports. No adhesive, just a very tight press in seal. I just pull out the filter and SHIP when I think they need replaced. It's so easy to do It doesn't feel right selling them.
@FreshfromtheFarmFungi
@FreshfromtheFarmFungi 5 ай бұрын
thanks for the info! You’d be surprised how many people can’t use a drill or just don’t have the time/patience 👍🍄❤️
@levittperez
@levittperez 7 ай бұрын
How would you sterilize between uses for the master lids with tubbing?
@johnnyfish6051
@johnnyfish6051 Жыл бұрын
How long would you say morchella Americana spores would remain viable in a dry climate controlled environment?
@user-oo4vi5iv3d
@user-oo4vi5iv3d 11 ай бұрын
I need your help please Haw can I prepare bromothymol blue stain?? I have the powder of bromothymol blue
@jhonnewton7433
@jhonnewton7433 Жыл бұрын
5bl Grain spawn bag is how many can use 5bl bags for block?
@EverythingCannabisTV
@EverythingCannabisTV Жыл бұрын
aye family i love these uploads keep them up please. you mentioned you like to make spore syringes with one of those style jars, I have an idea how to make liquid culture syringes, however for a spore syringe do you just put spores in water and fill the syringe? or how does the process of making a spore syringe differ from making a liquid culture. I know LC is the liquid mycelium, and spore syringe is the spore. Thank you again
@FreshfromtheFarmFungi
@FreshfromtheFarmFungi Жыл бұрын
Yes I either drop in a whole foil print or scrape spores off a slide and make it super concentrated
@Alisha-wr3os
@Alisha-wr3os Жыл бұрын
GARY!!! josh here (again) in Colorado springs.. you helped me find my flowhood (modular cleanrooms) and you were gonna come to my lab for some consulting.. Quick question for ya gary.. i have a tea business and i want to get on amazon but im not sure what app to use for my product thumbnails.. all of your thumbnails always look so awesome.. any advice on what apps to use? thanks again man.. ive been a subscriber and admirer of you for a few years now..i was subscriber 4,000 ish and im always sending my friends to your Etsy for gourmet strains etc..
@FreshfromtheFarmFungi
@FreshfromtheFarmFungi Жыл бұрын
thanks for following along! I just used microsoft word and imovie with my iphone - keep it simple and easy! 🙏🏻 I have an Imac for video editing and my macbook for all the thumbnails, titles etc. People on Fiverr helped me a lot in the beginning and then it took a lot of practice to get honed in. Some of my earlier ones are still up and quite different then the style now - MUSHLOVE
@GNARLOUSE
@GNARLOUSE Жыл бұрын
Thanks I needed this. My idea (what I'm presently using) of the early days is the original jar lids I modified with hobby lobby felt and silicone. I'm assuming all of the components of these lids are vulcanized right?
@FreshfromtheFarmFungi
@FreshfromtheFarmFungi Жыл бұрын
Im not sure but they lasted for 2.5 years
@GNARLOUSE
@GNARLOUSE Жыл бұрын
@@FreshfromtheFarmFungi nice, thanks again!
@jimycrackcorn408
@jimycrackcorn408 7 ай бұрын
yo Gary, what’s up with discount code for master LC lids? I watch your videos, religiously and over and over and over again whenever I need a reference, but I find a whole world of useful information from you and your company I appreciate all your hard work. I appreciate you putting this information out there for all of us to view and learn from I appreciate 🫵Thank you.🥰
@FreshfromtheFarmFungi
@FreshfromtheFarmFungi 7 ай бұрын
thanks! I don’t know of a code for the lids but I have some listed on ETSY and our ETSY code is FungiFam303 for $20 off any order over $120 etsy.com/shop/freshfungi 🍄❤️
@mycologylids
@mycologylids Ай бұрын
Use code FRESH10 for 10% off. Code is valid until 31Dec2024!
@baroncastanares2469
@baroncastanares2469 11 ай бұрын
Hey. I had a question about the advanced lid with the clip to prevent back flow. 1. How do you extract a full syringe of LC when there is air in the tube? When I try it only fills about 4cc 2. When you snap the clip to close the tubing, how do you expel the liquid in between the syringe and clip to prevent air in the system and prepare for subsequent LC extractions? Thanks!
@FreshfromtheFarmFungi
@FreshfromtheFarmFungi 11 ай бұрын
you can either ad a backflow preventer or just keep the hose kinked between syringes. Also, I will leave the last syringe in use on the end as a “label” and as a cap to keep air from entering the system. Things have calmed down a bit here I want to make a recap video soon
@dantepicantemartinez
@dantepicantemartinez 9 ай бұрын
I would love to see I recapping vid. I bought the master Lc lids and gave the jar with the lid bacteria. Im not sure where I’m the process I mess up but I’m sure it’s user error on my end.
@shaqx7039
@shaqx7039 8 ай бұрын
Where did you get your laminar flow hood from?
@FreshfromtheFarmFungi
@FreshfromtheFarmFungi 8 ай бұрын
we got one from air science and one from myers mushrooms
@cestlaviehappner1872
@cestlaviehappner1872 Жыл бұрын
So do you still wrap up your tops with aluminum foil or do you just let's set them in there like the ones for the syringes
@FreshfromtheFarmFungi
@FreshfromtheFarmFungi Жыл бұрын
no foil needed just be careful to not pull them by the tubing and it works well
@cestlaviehappner1872
@cestlaviehappner1872 Жыл бұрын
@@FreshfromtheFarmFungi so I'm assuming the syringe filters will be just fine into
@Shroomaholics
@Shroomaholics Жыл бұрын
Do you know what type of tubing is used for the lids?
@FreshfromtheFarmFungi
@FreshfromtheFarmFungi Жыл бұрын
it’s lab grade silicone I believe
@Mechaneer
@Mechaneer 5 ай бұрын
The one with the clamp...doesn't solution get stuck between the clamp and the tip where you insert the syringe, and then spill out when you pull the syringe off?
@FreshfromtheFarmFungi
@FreshfromtheFarmFungi 5 ай бұрын
no, there isn’t any back pressure the clamp acts as a backflow valve
@Chris-ru5hb
@Chris-ru5hb Жыл бұрын
How do the mycology lids tubing not get contaminated after pressure cooking?
@FreshfromtheFarmFungi
@FreshfromtheFarmFungi Жыл бұрын
if it’s already connected and capped off it will be sterile until it’s opened and then it’s a matter of doing proper aseptic technique
@johnhuynh248
@johnhuynh248 9 ай бұрын
Can the syringe displacement port sterilize ?
@edarrenellis
@edarrenellis Жыл бұрын
Gary, some of the parts on your list seem to only have a temp rating of 175F. Have you found this to not be a problem?
@FreshfromtheFarmFungi
@FreshfromtheFarmFungi Жыл бұрын
it shouldn’t matter unless it’s tubing, don’t use vinyl tubing but what I have listed is what Ive been using and it works great
@jeremekrug458
@jeremekrug458 4 ай бұрын
Have u posted links for the parts to make the lc lids
@FreshfromtheFarmFungi
@FreshfromtheFarmFungi 4 ай бұрын
they are on our amazon affiliate page
@MycoDogs
@MycoDogs Жыл бұрын
i was awake this morning
@FreshfromtheFarmFungi
@FreshfromtheFarmFungi Жыл бұрын
I have to wake up before the baby to do these now hah
@thekhibros7388
@thekhibros7388 Жыл бұрын
So you can PC these with the tubbing?
@mycologylids
@mycologylids Жыл бұрын
Yes
@bill6943
@bill6943 Жыл бұрын
Just ordered some Porcini LC. I have not been able to find it anywhere in Australia. Has be be the most I have ever paid for an LC. AU $68
@FreshfromtheFarmFungi
@FreshfromtheFarmFungi Жыл бұрын
it is an aggressive one - I am planting under some douglas fir this year
@FlatcapMushrooms
@FlatcapMushrooms Жыл бұрын
Hey how are your planted morels doing this morel season do a video please we live close in proximity and I’ve been trying new things too
@FreshfromtheFarmFungi
@FreshfromtheFarmFungi Жыл бұрын
still waiting 😬😬😬
@louisw5858
@louisw5858 Жыл бұрын
Small question:- while the culture is being preserved in fridge, is it necessary to do fresh transfers every 2-3 months as they run out of nutrients??
@josephconkwright4215
@josephconkwright4215 Жыл бұрын
I've had a culture of Nepal chitwan that made it in room temperature for two years so in the fridge it should be fine for at least longer than 3 months
@louisw5858
@louisw5858 Жыл бұрын
@@josephconkwright4215 so what is this transferring about?? I hear it often
@josephconkwright4215
@josephconkwright4215 Жыл бұрын
@@louisw5858 it's taking a s syringe and sucking some mycelium out of one liquid culture and then innoculating another jar of fresh liquid culture such as honey water or corn syrup water with the syringe.
@josephconkwright4215
@josephconkwright4215 Жыл бұрын
You can transfer to be safe and that's better but I don't think it's necessary as often as one might think
@FreshfromtheFarmFungi
@FreshfromtheFarmFungi Жыл бұрын
it’s not necessary the culture will regulate itself for a while
@DJ-xo4jg
@DJ-xo4jg Жыл бұрын
I'm sure your aware of the mono tub tek, and flushes. How do you feel about using grain water for misting and rehydration for flush?
@mybigsteaminjohn4027
@mybigsteaminjohn4027 6 ай бұрын
No.
@DJ-xo4jg
@DJ-xo4jg 6 ай бұрын
@mybigsteaminjohn4027 why? Isn't that what the fungi thrives on? That's why it grows in dung, it's the grain the animal was eating that supplies the food source.. or am I wrong? No. Doesn't answer a fucking thing, much less the original question.. now if you're nothing but a douche, well that's easy to see now.
@harrisonkane1077
@harrisonkane1077 Жыл бұрын
Love the vid! Unrelated question but do you have any thoughts, opinions or any hot-takes on why Paul Stamets wouldn't comment on the portabelo mushroom industry in his latest apearence on JRE ? Personally I I think he contains a dogwistle in his response for myco nerds saying in passing "its a very explose issue" from my very limited research I think its because portabellos contain a compound 'ageritine' which, apart from being carcinogenic can be used as an ingredient to make 💥?!? Pauls response to joe was really weird and exeptionally jarring. I also just cant believe that the gormet mushroom industry has 😴r agents ready to take someone out just at the mere mention of the potential issue but then again maybe im blissfully naive 🤷‍♀️ As i say i havent done a lot of research but though I would ask yourself as you're wayyyy more clued up on this whole sphere on knowledge than myself lol 😆 so yeah... TL;DR : Why are portabello mushrooms bad Gary ?
@jgarcia9734
@jgarcia9734 Жыл бұрын
What about aerating ur liquid culture with an air pump and filters. Almost like brewing a compost tea.
@fancycrafts7774
@fancycrafts7774 Жыл бұрын
Why though?
@FreshfromtheFarmFungi
@FreshfromtheFarmFungi Жыл бұрын
yes I have done this and am planning a system to scale it, there is risk with contamination but it helps grow it fast
@salovamrani2084
@salovamrani2084 Жыл бұрын
How to sterilize injection ports?
@FreshfromtheFarmFungi
@FreshfromtheFarmFungi Жыл бұрын
rubbing alcohol
@salovamrani2084
@salovamrani2084 Жыл бұрын
@@FreshfromtheFarmFungi is it goid enough? I read on the forums its not 100% reliable
@jbtravelssolo7596
@jbtravelssolo7596 3 ай бұрын
you do realize the back of those syringes are open and exposed and then you aspirate back and forth you are introducing contaminates right. 8:37
@FreshfromtheFarmFungi
@FreshfromtheFarmFungi 3 ай бұрын
Yes that is the whole point to displace the air while aspirating and filter it through .2 microns did you even watch the video?
@Alex-gb4vn
@Alex-gb4vn 8 ай бұрын
The problem with putting a syringe on immediately after inoculation is that the majority of the syringe is exposed to open air until you decide to take your first sample.
@VideoMatti90
@VideoMatti90 Жыл бұрын
I think mycolids is not selling these lids anymore...do you any other valid source to get them?
@FreshfromtheFarmFungi
@FreshfromtheFarmFungi Жыл бұрын
they may just be sold out
@FreshfromtheFarmFungi
@FreshfromtheFarmFungi Жыл бұрын
also, check the amazon page out all the parts are there to build your own
@VideoMatti90
@VideoMatti90 Жыл бұрын
@@FreshfromtheFarmFungi yeah i've seen these,but seems not that easy to build
@xMrSilver99x
@xMrSilver99x Жыл бұрын
find their origin at Spencers Shroomery
@graceyou237
@graceyou237 Жыл бұрын
@@xMrSilver99x they never ship, tried to order last month
@MrIzzy11B
@MrIzzy11B 8 ай бұрын
That’s nowhere near a half a gallon jar 🤣🤣🤣🤣🤣
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