Do you Have "Clean Spawn"?: Wet Rot, Hidden Contams, and How to Salvage Bacterial Spawn!

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Do you Have "Clean Spawn"?: Wet Rot, Hidden Contams, and How to Salvage Bacterial Spawn!

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Күн бұрын

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In this video, I go over common bacterial contaminations in mushroom cultivation. This is to help people see if they have any potential contaminants in their spawn. I primarily go over wet rot and hidden contaminations, plus how to go about fruiting/salvaging such spawn.
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Пікірлер: 388

@Mycophilia Жыл бұрын

Hitting the *LIKE* and dropping a *COMMENT* on a Mycophilia video has been known to increase flushing power to the MAX! It's true! 🎉🍄🍄🍄🍄🍄🍄🍄🍄🍄🍄🍄🍄🍄🍄🍄👽🛸 1) Patreon Replacement (memberships for videos and monthly genetics): tinyurl.com/mycosage 2) Mentorship and Genetics, I don't bite!: mycophilia.official@gmail.com 3) Discount codes in description 🍄💜 Sage

@jrewing3164 Жыл бұрын

I think I'm just going to clean all my stuff out start fresh with a new spore syringe it's been awhile I need to get some fruits

@ismaelrodarte876 Жыл бұрын

Hey sage quick question I’ve recently had a problem where my jars colonize everywhere except the tops of the grain they just reach out there and seem to stall any idea on why this might be? And is there anything I can do to prevent it from happening again thank you very much very grateful and thankful for your videos 🤝💙

@kroketet 10 ай бұрын

Great vid

@unicorntheory312 9 ай бұрын

Hi sage, I need grain spawn bags, do you have a referral? Thanks

@GarnettRiverProductions5836 8 ай бұрын

your Canadian? eh 🍁

@Humboldtcounty707 4 ай бұрын

The jar that won't break-up is really good info! If your mycelium doesn't break up easily then you're going to have problems. Took me a while to figure that one out.

@DARKW1ZARD616 2 ай бұрын

I recently broke up couple spawn bags all of them were kinda hard should I be concerned?

@XxxXxx-dc3ud 18 күн бұрын

Thanks for sharing this information . Bless you and your family

@MCH400 8 күн бұрын

@@DARKW1ZARD616 I've personally never had this issue it just looks overly colonized the me but that's not a problem in my experience. I'm thinking I'm maybe not seeing the texture he's trying to show off

@Vodafone16v Жыл бұрын

Duuuude!!! Just struck GOLD finding this video!!! Been struggling with bacterial riders for a whiiiile now. Basically have narrowed it down to my LFH filter been saturated, and every "open jar" agar/grain transfer I do in front of it gets bacteria that I later transfer to tubs and get Trich. Really frustrating cuz the myc looks "OK" but at the end of the cycle, all that effort and resources are lost. But, looking at your video I've learned that they may ALSO be embedded bacteria coming in the genetics purchased. 🤔 Cheers man! Keep doing these videos! One about possible origins of such contams/bacteria would be nice!!!

@Mycophilia Жыл бұрын

Thanks man, talk about serendipity. I’m currently editing a video where I briefly talk about one of the sources of such contams. Should be coming out today!

@XxxXxx-dc3ud 18 күн бұрын

Good shout bless you and your family

@SketchybrainD 9 ай бұрын

Ah man, My first grow I did 10 jars and I didn’t get one that was completely clean. I was able to salvage enough to do one tub of gt And it worked. Got a bunch of really small ones the first flush, I was so happy it worked. It was mss straight to grain and I wasn’t very good at hydrating the popcorn at all. Plus I don’t think I did even a full 90 mins.

@bootybootylove Жыл бұрын

I’m new like Joe Dirt, but vids like these are super helpful when getting into something new. Learning the different failure modes and what you can or can’t do about them is so important to any process. Very much appreciate the knowledge, the prime examples, and the information! 🍄

@Mycophilia Жыл бұрын

Thank you Mark 😊

@1871corporationUSA Жыл бұрын

Use water agar. Or cut out a channel in your agar so the mycelium can escape the bacteria by jumping the chasm. Cleaned some s.n.a.p.e culture up via these two methods in one transfer

@Mycophilia Жыл бұрын

You were able to clean up embedded bacteria with water agar? Or regular bacteria?

@MCH400 8 күн бұрын

That's a phenomenal idea actually. Smart. The mycelium will reach across while the bacteria is held on the contaminated side 🎉🎉🎉🎉

@MCH400 8 күн бұрын

@@Mycophiliahe means embedded bacteria. Basically youd leave 1/3 of the dish with the bacteria embedded mycellium then empty the rest out and fill with fresh agar alongside that wedge and then cut a "ditch" out of the agar so there is a line of clear space between what's already colonized with the bacterial mycelium and the side with the fresh agar. The mycelium will walk over that gap but the bacteria in theory wouldn't transfer along with it. I haven't personally had to do this, but it certainly does make a lot of sense

@Mycophilia 8 күн бұрын

@@MCH400 Yeah it's called the "trenching" method, and I haven't tried it myself either (it wasn't a thing when I made this video). Plan on trying the next time I see an issue with embedded contam 👍

@kaandemirkiran2954 Жыл бұрын

my fav youtube chanel so far

@Mycophilia Жыл бұрын

💜 thanks 🍄

@joeby5438 4 ай бұрын

Thanks for this content, its hard to find information on hidden contamination, but it's quite common for me! One idea I wanna share with you @Mycophilia: I remember Mycoplasma are smaller then other bacteria and prefer to stay intracellular, not intercellular. Maybe they are your hidden embedded contamination. Did you try growing this kind of contaminated mycelium on Agar-water? all the best!

@Mycophilia 4 ай бұрын

Certainly could be the case. As for water agar, yes, and ime it does nothing for embedded contams unfortunately

@PovertyStudios Жыл бұрын

Trich kinda takes over in growth quickly, if it's spotting light green it's penicillium, trich spreads much faster and has a forest green color when matured, penicillium takes longer so you can let it go a little longer before you have to toss it out, gives you chance to get some fruits out if you're at that point that is. Love the vids brotha!!! Keep fighting the good fight!!! 🦍🙏💪🏿

@Mycophilia Жыл бұрын

👍 👍

@nattybizzle 11 ай бұрын

You can eat contamed fruit?

@PovertyStudios 11 ай бұрын

@@nattybizzle depends how contaminated I guess lol, you mean like if you picked fruits from a tub that was contaminated? Or do you mean if the fruit itself has contamination on it? Either way best case scenario is you get diarrhea, worst case is you get sick and die lol but i would use the fruit from a contaminated grow to try to clean up into a healthy culture for future purposes if that's what my objective was at the time.

@DocR16 Жыл бұрын

I'm in the process of building a professional lab, so if you want to keep me in mind towards the end of 2023, I can run some tests on that one spawn jar that you can't identify the mold/foreign spores that keeps ruining your grow

@FederalBeureauInvestigations 10 ай бұрын

FBI

@thelvadamee3767 8 ай бұрын

What is blud wafflin about

@user-py7rf7gz5c 3 ай бұрын

Have u tried blastinguvc sterilizing light myco survives the bactia is bleached works best on colonized agar and liquid culture

@LonnieLunsford Ай бұрын

@@user-py7rf7gz5c what is the tech that you use for the UVC light?

@kevandgizmo917 7 ай бұрын

This video has answered so many questions for me and new avenues to pursue which I thought were ended, thank you for this

@ZombieSnax42069 4 ай бұрын

Did it scare you away or make you spend 250$ on cleaning supplies and protection ?

@sethbaker5261 3 ай бұрын

I'd love a video on how to use a microscope to verify grain spawn. I've seen very few 'scopes while perusing KZfaq myco tutorials. Also, thank you for the tip regarding pressure cooking contaminated grains. I've been driving a mile away to dump, still obviously bringing spores home on my clothes.

@andyshelly3473 Жыл бұрын

i learned something new , ive had this . at the very leading edge of the mycelium on agar you can see a faint hays . i have a fruit on the petri dish , so im going to collect the cap and make a new spore print .

@melissalovesnature3370 Жыл бұрын

Great video! Now I know why I keep getting failures with my latest batches. Time to go back to spore.

@Mycophilia Жыл бұрын

Thanks Melissa for the comment!

@beentheredonethatoriginals5673 Жыл бұрын

Glad I found your channel. I have a year old Oyster jar of spawn that is tightly grown in and has several tablespoons of liquid in it. It was pushed to the back of the fridge and forgotten. I'm gonna give it a shot, thanks for the info it looks like it might not be contam after all.

@Mycophilia Жыл бұрын

That sounds like bacteria to me but I’d give it a shot anyways!

@Mycophilia Жыл бұрын

Glad you found my channel, hope you enjoy!

@metamike2012 Жыл бұрын

That explains a lot. I have to cut my spawn out of the jars. There's no breaking it up. All my agar looks great ! At T -7 now starting to get ropey but put to grain it stalls out . If I shake it, it doesn't recover. Time to top fruit for some new spores. Thanks !

@Mycophilia Жыл бұрын

🍀 💜

@kgproductions273 Жыл бұрын

7 transfers?

@Somecallme_Hud Жыл бұрын

Thank you for putting this together.

@Mycophilia Жыл бұрын

My pleasure Hudman, glad you enjoy!

@kiowhatta1 Жыл бұрын

I find Trich has a greyish hue, colonises very quickly and looks like cotton balls, but mostly it doesn’t have the beautiful alabaster white shiny complexion of mycelium.

@Mycophilia Жыл бұрын

A lot of trich is gray, but they also go super white just as much. Waaaay whiter than mycelium, usually before turning green.

@kristhomas6474 2 ай бұрын

Thanks for the video. Very informative and helpful. I appreciate it Im a beginner and having lots of trouble and videos like this one is invaluable for me

@Mycophilia 2 ай бұрын

Glad it was helpful!

@martinmadrid3192 Жыл бұрын

Mold infects plants and fungi systematically, it has me question if some bacterial infections aren’t the same. Thank you for sharing so much information 🙏

@Mycophilia Жыл бұрын

Thank you for watching :)

@ronnienoneyabusiness2844 Жыл бұрын

I dig your channel man...doing gods work

@Mycophilia Жыл бұрын

Thank you 😊

@VincenzoC749 Жыл бұрын

I loved the video, but I am not really sure what to look for. I see the little yellow metabolites, and the fluffy white jars definitely look strange. But I think I just need to do more research on contam issues to get a better free asp of the possible mycelium pitfalls that you’re likely to encounter, compared to healthy growth.

@Mycophilia Жыл бұрын

After a few grows you will begin to see what healthy mycelium looks like and unhealthy mycelium looks like. It’ll all fit together so have fun while you’re at it!

@fredrikvang Жыл бұрын

Very interesting, but what makes you think the jars with the white mycelium are contaminated with bacteria, or even contaminated at all? Have you done some tests that support that notion? Might not something else actually be going on? (BTW, next time why don't you slice the white mycelial mass into 1.5-2 cm 'breadslices' with a knife and try putting them btw a thin 50-50 CC-Verm bottom and thicker 50-50 CC-verm top layer and see what happens. I suspect breaking up spawn that looks like that might not work very well.)

@Mycophilia Жыл бұрын

They are 💯 contaminated, once you’ve grown for some time you know what’s contaminated and what’s not. I have listed a number of reasons on this video, the primary ones being the extremely thick growth that makes it impossible to break up, along with all previous attempts with this culture always ending up with the same green mold, at least those that have been spawned to bulk. The ones that have not (bottle fruited), have not fruited in 2+ months of fruiting conditions, in addition to growing very strange mycelium masses after the first month, a hallmark of contamination. As for your idea of slicing, a better method exists called jar/top fruiting. It is better because you don’t need to break up the mycelium at all. Hope that helps.

@Enochulate88 6 ай бұрын

By the smell lololol

@gelhope5510 Ай бұрын

@Enochulate88 he said it didn't have the smell.

@DoseofScienceDoS Жыл бұрын

If your bulk is going bad then you have 1) Not fully sterilized (longer higher temps required) Why does this happen???? Elevation has an effect on temp. 2) You introduced a contaminant (Lysol/alcohol and draft hood) 3) Your inoculate is contaminated (do a better job on sterilizing and use smaller wide mouth jars)

@Mycophilia Жыл бұрын

It’s 3, I cover that in the video. Thanks for the comment!

@ericwilkerson4912 Жыл бұрын

I'm really glad to find this video today because I have a big tub exactly like that

@Mycophilia Жыл бұрын

Glad to hear that!

@gawdzalien2811 Жыл бұрын

One day I'll be ready for this

@bracedforpleasure 9 ай бұрын

Have you looked into hydrogen peroxide flushing your substrates and grains before inoc or bulking?? Also with certain contam caught early enough it can treat bulked tubs by spraying and wiping up.

@Mycophilia 9 ай бұрын

Nope peroxide should never be used that way.

@bracedforpleasure 9 ай бұрын

@@Mycophilia I'm genuinely curious Why is that?? I can't say I've heard of any negative effects from either method. But there's a lot I don't know so any help is much appreciated.

@Biokemist-o3k Ай бұрын

Another banger video my brother!!!

@TheStoneMan.Official Жыл бұрын

Cleared up a lot here good timing on this topic!!! Thank you so much Sage always learning more from you!

@Mycophilia Жыл бұрын

Glad it was helpful!

@RickL555 Жыл бұрын

Great vid man. I stopped using WBS because some bacteria can burro deep in low grade grains and can survive the pressure cooker. Keen eye on that particularly deceptive agar plate. I wouldn't have caught that one in a million years. Patches of mycelium that grow little spikes are another sign to look out for. Keep up the good work, homie.

@Mycophilia Жыл бұрын

Thanks, appreciate it 💜

@Animal-Reaction-Clips Жыл бұрын

So what is the best thing to use then? Thanks

@consolechips Жыл бұрын

If you soak the grains for 48 hours you can germinate the bacterial endospores and this way they will be killed in the pressure cooker.

@RickL555 Жыл бұрын

@@consolechips Right on, Thanks. That makes perfect sense.

@GNARLOUSE Жыл бұрын

@The Eye-Rollers Guide to the Galaxy haha just watched your vid about this! Embedded bacteria huh? Well guys live and learn...sigh. What in Your guys opinion would be the bullet proof medium to avoid all this? Thanks to both of you

@rebeccaambrosino1191 Жыл бұрын

Love this video content. Very helpful. Thank you so much! Subscribed!

@Mycophilia Жыл бұрын

Thank you 😊

@mj_awesome8925 Жыл бұрын

Amazing video, very informative :D Mush love

@Mycophilia Жыл бұрын

Thanks MJ 💜

@allseeingguy5485 Жыл бұрын

I had no idea that the mycelium itself could be contaminated. I had this problem for many runs and took a break because of it.

@Mycophilia Жыл бұрын

Something that isn’t talked about enough!

@lelilimon Жыл бұрын

Use plates with multiple compartments for SN-T1 2/6. Mycelium climbs over walls to section with clean agar and there's your clean wedge, success is like 99.999%. Just be very sure not to spill agar on walls.

@Mycophilia Жыл бұрын

I like that idea for regular bacteria but with embedded bacteria like this, I can’t imagine it helping as the contam exists within the mycelium

@lelilimon Жыл бұрын

@@Mycophilia i had same problem and climbing mycelium was the only way in my case

@ethankaufman8577 11 ай бұрын

@@Mycophiliahave you tried water agar? The mycelium Will stretch out and look for nutrients while the bacteria won't have an ability to spread without nutrients. I've been able to cleanly transfer bacterially contaminated mycelium after one or two water agar transfers

@ebtsoby Жыл бұрын

never heard of embedded bacteria, I thought if it was clean on agar it's clean period, thanks for the vid

@Channel-tl3xz Жыл бұрын

Those "embedded bacterial" jars are not actually contaminated. I think he's mistaking crappy genetics. Those don't look bad at all. There's no "tell tale" sign of anything in those jars

@ovrjoyd Жыл бұрын

Excellent and informative content 🤗

@Mycophilia Жыл бұрын

Thanks Ovrjoyd 💜

@ianbabineaux2050 Жыл бұрын

Finally a video with good information.

@Mycophilia Жыл бұрын

Cheers thanks for your comment 😊

@Lucas-jp7uz Жыл бұрын

I have a tub that looks like the jar you said would become trich. I also think my tub is going to turn to trich, after seeing this though I am hopeful because it's passed the time other tubs usually turn green. We will see though, probably just jinxed myself lol

@oldboots75 Жыл бұрын

very useful info 👍 cheers Sage

@Mycophilia Жыл бұрын

Glad it was helpful!

@mysticmoth1111 Жыл бұрын

what i gotta go down this rabbit hole

@TheQuixoticRose 11 ай бұрын

Omg that really white mycelium is something I thought was healthy and it's true right after the first flush they contam 😭 or even during the first one and I only get two fruits

@beekeeperd.7656 Жыл бұрын

Is this all you got? lol. I was hoping to get nightmares from a mutant Blob consuming the city. I grow Tric just for fun (or so I tell myself). Sorry but my contaminants would eat yours for breakfast. Aspergrillis is my homeboy. It's not a grow till I see happy knats greeting me! But seriously folks... Good stuff. I see wet rot too often in my oat grain jars. Thanks for good info!!

@Mycophilia Жыл бұрын

Oh no, not the aspergillus! Thanks for the comment Beekeeper!

@Unclemushlovin Жыл бұрын

Cheers new channel and new to growing mushrooms good info thanks

@Mycophilia Жыл бұрын

🍄 💜

@davidcora2751 2 күн бұрын

Small changes to variables is useful to determine a proper path ? Is this the reason one of three had different outcomes?Thanks for informative science on these fascinating life species💕❤️

@kiowhatta1 Жыл бұрын

Why one would bother with PF tek anymore is beyond me. But each to there own. First I had to pressure cook the jars, drill holes then squirt 2ml of liquid spawn into each jar. I did 4 jars, lost two cakes to bacteria, then put the other two in a tub with perlite on foil. (It took a month to colonise the jars btw). One cake produced a few fruits then that was it. I’ll never do it again.

@thesupreme7815 3 ай бұрын

What do you do now

@ineffably_described Жыл бұрын

Thank you! I just spawned a bacterial B+ to a shoebox with a 1:1 ratio. It looks great but I will have an eye on it. Had to scoop out the grains but they smelled wonderful still.

@Mycophilia Жыл бұрын

Best of luck! 🤞

@user-xr5cz2mr4v Жыл бұрын

@@Mycophilia If you have a few bacterial spots on the outside, cant you rub the spawn until its sides are rubbed off and just bulk the middle part, which should be clean because it has its stable immune system.?

@1871corporationUSA Жыл бұрын

@@user-xr5cz2mr4vno, the bacteria transfers via water, humidity. Culture has to be cleaned up via water agar, or multiple transfers on regular nutrient agar with chasms sliced into the agar to impede bacterial spread

@biker_one208 7 ай бұрын

Üblicher Weise fertigt man aus dem Petrischalenansatz erst einmal Flüssigmycel an. Dadurch lassen sich viele Probleme mit Kontaminationen vermeiden.

@DangerRussDayZ6533 10 ай бұрын

Every jar I've ever tried to spawn has turned into those thick bacterial contam but fully colonized / smells fine jars. I've been thinking it's the WBS I've been using and/or the holes in my modified lids aren't large enough.

@Enochulate88 6 ай бұрын

You should see how small premade holes are. Like a screw driver or nail size

@Enochulate88 6 ай бұрын

You should see how small premade holes are. Like a screw driver or nail size

@Mustachedmyco 6 ай бұрын

Heey homie i Had nothing but problems with fliped lid tek!!! 😞 Switched to ball brand plastic lids with 1 , 1/4 inch hole , covered with mirco pore tape i use 2 layers! Grab some low nutrient antibiotic agar if ya haven't already those plates will clean up 🔥🤙 🍄

@Mycophilia 6 ай бұрын

Nothing to do with flipped lid here. But I appreciate your comment nonetheless. Antibiotic agar is also not a cure all. Most times, it will only accomplish suppressing the contamination temporarily, but once put to grain or a non-antibiotic plate, the bacteria will be back.

@burgerbobbelcher 11 ай бұрын

You said "these two guys are full of truffles". What does that mean? Is that an insider term for some kind of contaminant I haven't heard of? Or have you actually discovered a way to cultivate truffles?

@wildernessfarming7726 5 күн бұрын

i think hes talking about the kind of truffles you find at headshops in ansterdam. not a new phenomenon.

@CarolynsLoveRickshaw Жыл бұрын

How do you recommend disposing of contaminated spawn? Thanks for the great content.

@Mycophilia Жыл бұрын

kzfaq.info/get/bejne/fLCdi7l9vtadZWg.html

@Animal-Reaction-Clips Жыл бұрын

Chuck in in your neighbour dish

@stevenmoody-MoodyHivesandHome Жыл бұрын

Awesome video man.

@Mycophilia Жыл бұрын

Thanks Steven 💜

@DanSetzer 7 ай бұрын

You got your tea right beside bacteria colonies 😮😮😮❤ keep it up watch that tea xd

@Frittierer.111 9 ай бұрын

I am in the same Situation right now.. Mycelium looks really thick and a lot of White dots and uneven, not flat at all.. but no bad smell at all... I still made a bulk in a monotub, but I don't know if it works.. I have got an orange touch and some dry powder from some jars.. I assumed it to be Penicillin, but who knows!!

@Mycophilia 9 ай бұрын

Powder and orange are a no-go

@Frittierer.111 9 ай бұрын

@@Mycophilia yes, but is it Penicillin, because it has no smell. It actually smells fresh?!

@Frittierer.111 9 ай бұрын

@@Mycophilia the Orange is like the colour from oyster mycelium, more white, it just looks like that in the reflection, only slightly!

@adammiller6747 Жыл бұрын

Bro❤❤❤❤ i fucking love yer plates😊 absolutely gorgeous

@MB-sn8sf 5 ай бұрын

12:19 in those situations the best best is WATER AGAR

@Mycophilia 5 ай бұрын

Unfortunately water agar doesn’t do anything for embedded contams

@kushgardenr1652 Жыл бұрын

I had a few fully colonized jars of grain and they didn't appear to have any contamination. but there was a little bit of water in the jars after they were fully colonized. But I opened them to spawn them to bulk and they all smelled good like fresh mushrooms. Do you think they should be fine ?

@Mycophilia Жыл бұрын

Probably, only so much I have to go on with that description but I’d definitely spawn it either way

@charlesdarwin4780 Жыл бұрын

Very informative, thank you!

@sunnydaze5721 Ай бұрын

Hi❤ Such a great video for a beginner Ty xoxoxo 😊

@SparQz Жыл бұрын

You need a flow hood or at least a still air box... it'll cut your contams way down.

@Mycophilia Жыл бұрын

I have both. Hidden contams are not really influenced by sterile technique as the mycelium itself is contaminated.

@Donny_B Жыл бұрын

I wish I would have seen this yesterday. I had one jar that wasn't quite as far as the other. Went to bulk with them both....together....in the same tub. Both were full of mycelium but one just looked better. They smelled earthy. Had no reason to think they were bad in any way. Judging by your video I may have messed up. What should I look for? Is there a chance it's fine? I feel like a bad parent. These are my babies 😢

@Mycophilia Жыл бұрын

There is hope, they may be completely fine. Only one way to tell

@Donny_B Жыл бұрын

@@Mycophilia only way out of it is through it! Full steam ahead!

@danwilkinson2797 Жыл бұрын

Those overlaying myceliums often look quite funky such as the ps. Nat

@Mycophilia Жыл бұрын

I got some real overlay on a mckenaii tub

@pequiventania9658 5 ай бұрын

After scaling up my grow I got a lot of bacterial contam, still trying to rule it out. I was using popcorn, never had a problem before while using jars, but when I moved to bags I got into problems, all of my contams are bacterial, not a single colorful mold :( Now im using millet, perhaps it will be easier to achieve clean spawn.

@seanhenderson1522 Жыл бұрын

Im curious why don't you use water agar to isolate the Mycelium from the bacteria ???

@Mycophilia Жыл бұрын

Because water agar does nothing to embedded contams as the mycelium itself is contaminated, it’s not something you can easily isolate like regular bacteria via water agar.

@seanhenderson1522 Жыл бұрын

This would be the first I have heard of “embedded” bacteria that can not be removed. Can you kill it with antibiotics? It seems like you’re suggesting that there is a bacteria that is living and feeding from the mycelium it’s self, definitely seems plausible.

@mikeinnj4424 Жыл бұрын

Great video! Will keeping my grains at a slightly cooler temp help deter the bacterial growth and allow the mycelium to overtake it?

@Mycophilia Жыл бұрын

It’ll help deter bacterial growth, but if the bacteria’s already ‘triggered’ then it won’t make much difference as the mycelium will always lose eventually

@ebtsoby Жыл бұрын

I had a box that formed hard nodes all over the surface and wouldn't fruit, never seen something like that before, smelt great tho even when I broke it up and threw it away, wonder if it was some sort of embedded bacteria, I got rid of all the grain cause I tried to fruit it 4 times and gave it a month each time no success

@Lipi19821 Жыл бұрын

I would never thought that unbreakable mycelium is contaminated...I am no expert tho.... maybe anti bacterial agar would help... make clean coulture...?

@MrPro-hg8dd Жыл бұрын

Awesome video very helpful thanks bro

@Mycophilia Жыл бұрын

Thanks for the comment, glad you found it helpful Mr. Pro!

@conormarie4310 11 ай бұрын

interesting I've probably come across that without realizing what it was.

@WhoDeyWildcatsReds 8 ай бұрын

Question, were those white jars of the compressed myc started from what looked like a singular culture on an agar plate from spores? I think people get germinating spores mixed up at times. Spores can germinate and grow by themselves. They only need to meet up later with other germinated spores to go on and grow healthy mycelium and eventually fruit. This may be the problem with that myc. I'd try introducing another sample of myc that came from the same strain and see of they marry 🤷

@Mycophilia 8 ай бұрын

@johneywangers1425 Жыл бұрын

I'm when I can get a set up figured out I'm gonna look at mutations with variable mutates like bacteria uv exposer ect...

@RandomsFandom Жыл бұрын

Steam your jars longer, or test out adding ashes. Some mushrooms bloom best after a fire

@Lexington-Felix 10 ай бұрын

have you added ashes just wanting to know if your hypothesis works ? i personally wanna try those jelly-looking colored balls that you soak in water till they become the size of a marble after 24 hrs later. people use them inplace of soil to grow most houseplants anyway i was thinking just adding a few dry balls when they are the same size of rice and it works just like white rice to suck the moisture out of your cell phone. like a silica packet does.idk ill let you know how it works 🙂

@RandomsFandom 10 ай бұрын

@@Lexington-Felix orbees only hold moisture, and you have to use nutrients to keep a plant alive. Along with frequent replacement of the spheres of polymer and water. Mushrooms are very hard to grow outside of their natural environment. In nature they can survive other molds and bacteria. In the lab they require a sterile environment.

@kingmetroplex Жыл бұрын

Great video ❤

@peppergrowmienola4850 Жыл бұрын

Good info. I have dealt with a bit of contamination over the years.

@vincentwu2848 Жыл бұрын

Hey! I think I'm dealing with the same bacteria as you. I've also done multiple transfers to both agar and grain and still am seeing those big fluffy clumps on top. Do you know if it's definitely bacterial, or could it be a mold or yeast? If it's bacterial then perhaps a transfer to antibiotic agar could clean it up?

@Mycophilia Жыл бұрын

Definitely worth a try. I would recommend doing at 2-3 xfers on antibiotic agar, and then 2 xfers on regular agar to make sure it won’t come back. This is because bacteria will always eventually outsmart the antibiotics eventually

@Mycophilia Жыл бұрын

It’s definitely not mold, that much I can say. Yeast is possible as well, although I can’t say for certain.

@AndreaDingbatt Жыл бұрын

Thank you very much for sharing your thoughts and knowledge!!👍 Much Appreciated,💯 Andrea and Critters. ...XxX....

@Mycophilia Жыл бұрын

🍄 💜

@TheYoungWolf Жыл бұрын

Those super white is perfect...you don't want any other colors......

@Mycophilia Жыл бұрын

Contammed.

@Suspendedanimation007 2 күн бұрын

Hey I had a grain bag w some wet rot and a lot of v healthy mycelium. I mixed it into substrate and it did smell for a bit but now the smell is gone and the cake looks amazing. Should I be worried about the fruits having some toxins from the wet rot? Can the mycelium eat the bacillus and any toxins they would have thrown? Any tips on what to do w the fruits? I have a feeling they are going to “look” v healthy and don’t want to waste the bag I’ve had a hard time salvaging this strain! Thank u!!!

@Stodrag Жыл бұрын

worst is to clean the jars bacterial is fine only weird smell but the mold ew.

@its--_--me Жыл бұрын

Apologies if you've already addressed this in a previous video, but when fruiting truffle producers, will the fruits be more potent the longer i let the spawn colonize? Or as soon as the jars is colonized(little to no truffles forming) spawning to fruit will be the same potency?

@MattRoadhouse 8 ай бұрын

the fruits are tiny and potent as it is. Recent run was 6 months truffle then fruiting trays with peatmoss casing, but I'm going straight fruiting for Tampa / Mexi this round. Will see if they are in the same ballpark.

@timbowen8749 Жыл бұрын

For some reason I have better luck with rye berries than brown rice, and I've tried the 90 second uncle Ben tek and no luck! I noticed that the uncle Ben had vegetable oil added and thought that was the reason! I've had good luck in the past and now not so much! Maybe I buy presterilized rye bags next! I'm talking suggestions lol! Also looking for the kit for making liquid mycelium

@Mycophilia Жыл бұрын

UB rice bags are pretty wet, which seems to be the cause of contams for many ppl with that TEK. Many recommend off-brand instant rice for this reason, maybe worth a try? 😊

@UrMothersLoverr Жыл бұрын

look into mgp and try that it should help with your bacterial and trich issues

@seionthreat2644 Жыл бұрын

A window into the thickness

@ST1CKF1SHGAMING Жыл бұрын

Ive just started out and did as much of the sterilisation I could with everything. I did an oyster batch but looked like white fuzzy mold starting after a few days so got rid of it also those random white dots in various places. Then I redid the process trying to be even more careful with another LC syringe (GT) I got in a pack. Was as sterile as i could be with alcohol, etc but on rye and oven baked at 80 deg C for about 2.5 hrs since i dont have a PC yet. Made sure to squirt down side to monitor, after 3 days I can see white either whispy or early signs of white fuzz. Should i leave it and monitor or perhaps toss it while its pretty early? Appreciate the content and learning a ton so far.

@ST1CKF1SHGAMING Жыл бұрын

Ok ignore previous comment I watched your rhizo video and my mind is at ease now. I wish we could show pics here so you can see how my grows are coming along thanks to your advice. Keep at it love the info ❤

@Mycophilia Жыл бұрын

Glad to hear that 😊 best of luck!

@foxybuddy 7 ай бұрын

What exactly the smell end up like if that's not contaminated? I've done my first try, the mycelium bottle smelled like wine, quite strong when breathing in, suppose I should wear mask before opening the lid

@HellGod67 Жыл бұрын

Where can I get the truffle culture and how it's called?

@sacacorchosbarravel4909 Жыл бұрын

Been there, done that. Great vid..

@Mycophilia Жыл бұрын

Thanks!

@jamesrapkins4935 Жыл бұрын

Thanks for the good oil! i have a similar situation with a H.PES genetic... i was sure its carrying something, but i cant see it on agar. there is a new tek available that is being tested in the community btw. Rit dye agar.... the other option would perhaps be water agar ?

@Mycophilia Жыл бұрын

I’ll make an update if anything works!

@Animal-Reaction-Clips Жыл бұрын

Why not just keep it simple, it's fungus it'll grow on shit if you leave it alone.

@Shitstain951 Жыл бұрын

@@Animal-Reaction-Clips lmao you’re one of those morons. Okay then explain how one would “keep it simple” when their cultures are contaminated? Dirty straight from the vendor?

@fernetstock1 Жыл бұрын

I think I have the same problem. Transfer through antibiotic agar could help with those embedded bacteria (gentamicin, chloramfenicol)?

@Mycophilia Жыл бұрын

It should, I would recommend doing 2-3 xfers on antibiotic bacteria, then 2 transfers on normal agar to make sure it doesn’t come back (as bacteria will always overcome the antibiotics eventually, so you need to make sure they’re gone for sure before putting it to grain)

@milwaukeebuds Жыл бұрын

Thank you! 🫡

@gangsterbuilder Жыл бұрын

Air active bacteria.. I want to grow grass on top of truffles as a substrate

@CozyManiak 11 ай бұрын

I’m having that issue with some strains too; clean growth on petri but contam when I transfert them. I noticed a very very thin growth underlying the mycelium on petri and it’s always growing just a little bit ahead of mycelium The growth pattern is more chaotic than mycelium and I need to look very close with bright light to see it. I think it’s parasitic. I wish I could identify, all I know is that it’s probably not gram negative, as I tried to transfert from petri to LC with high dose of gentamicin.

@kenbourekas420 9 ай бұрын

I started doing this chasing mycelium into fruit about a year ago and I use the uncle Ben’s method but the last six syringes I received all turned into super green nasty. My guess is that it’s Trichoderma has a very foul smell to it. my first I’d say eight times was very successful. I have not changed my procedures if anything I have gotten even more excessive compulsive about cleanliness. I am guessing that I am getting contaminated syringes. From your experience, is that a possibility?

@Mycophilia 9 ай бұрын

Absolutely

@loganmontes9 3 ай бұрын

In regards to the bacterial spawn, my grow bag has looked like that since inoculation. I was informed it was “tomentose mycelium”. It’s grown fine and now It’s in its 3rd week of fruiting conditions, not much has changed. I’m starting to get concerned that it may never fruit. What are your thoughts?

@Mycophilia 3 ай бұрын

3 weeks and no sign of fruit is very slow. From my experience, I would definitely bet on bacteria being the case.

@ifell3 Жыл бұрын

I've had a blue cheese smell before 😮

@looneycrow7978 Жыл бұрын

Well done video

@Mycophilia Жыл бұрын

Thank you, appreciate it!

@HumanOddity69 Жыл бұрын

What if instead of breaking them up you used wide mouth jars and just bury them in substrate?

@Mycophilia Жыл бұрын

as in top-fruiting? If so I cover that in this video.

@HumanOddity69 Жыл бұрын

@@Mycophilia Not exactly, I was thinking of doing it in a tub.

@HumanOddity69 Жыл бұрын

@@Mycophilia Wide mouth just to get the cakes out easy.

@ows1993 Жыл бұрын

Hey great video! I am doing LC's for my tranfers to grain and it seems i get this thick white mycileum along the sides of my jars, but the center is pretty easy to break up and not thick at all. Does that sound like that bacteria or just mycelium ?

@Mycophilia Жыл бұрын

Thank you, if it’s easy to break up that is a good sign that it’s not contaminated. The only way to truly tell if it is clean is to just try using it.

@ows1993 Жыл бұрын

Thank you for the quick response, much appreciated. I will se how they work out 🙂

@aswfishing4319 Жыл бұрын

Dang my PE jars got those blobs:(

@Mycophilia Жыл бұрын

PE naturally form blobs, especially in first flush!

@1To4IlD Жыл бұрын

Where can I get ahold of You, personally? I've got some Grains I'd like You to see so You can possibly help me decide on what it is & the nxt steps I'll be needin to take. Also, THANK YOU for all the GREAT ADVICE & KNOWLEDGE that You KINDLY SHARE w ALL OF US!!! 🤙🏽😎🤝🏽

@Mycophilia Жыл бұрын

Hey 1T thanks for watching and commenting! I currently only do private questions in my mentorship program. Otherwise you can post your questions on my subreddit or discord server (you can tag me with your picture with @mycosage and I will come take a look).

@1To4IlD Жыл бұрын

@@Mycophilia Okay, cool. Thank you!