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COVID19 -SARS CoV 2 detection by rt-PCR
Polymerase chain Reaction
KARRY MULLIS in 1984 introduced PCR and got the Nobel prize.
Definition- It is a cell free technique for amplification of DNA molecule
Requirement- Thermal cycler, Taq DNA Polymerase, dNTP, Primers, DNA template
Steps- Denaturation, Annealing and Extension
Applications
Detection of viral infections
Diagnosis of Tb
Diagnosis of cancers
Prenatal diagnosis of diseases
Forensic medicine
COVID19
rt-PCR
Real time PCR
Taqman probe
SYBR green