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PCR, rt-PCR and Real time PCR

  Рет қаралды 224,284

Biochemistry by Dr Rajesh Jambhulkar

Biochemistry by Dr Rajesh Jambhulkar

Күн бұрын

COVID19 -SARS CoV 2 detection by rt-PCR
Polymerase chain Reaction
KARRY MULLIS in 1984 introduced PCR and got the Nobel prize.
Definition- It is a cell free technique for amplification of DNA molecule
Requirement- Thermal cycler, Taq DNA Polymerase, dNTP, Primers, DNA template
Steps- Denaturation, Annealing and Extension
Applications
Detection of viral infections
Diagnosis of Tb
Diagnosis of cancers
Prenatal diagnosis of diseases
Forensic medicine
COVID19
rt-PCR
Real time PCR
Taqman probe
SYBR green

Пікірлер: 135
@DrAngryFace
@DrAngryFace Жыл бұрын
*The way you teach can memorize entire Biochem even for an Alzheimer's patient* 😹🫶
@sandhyarathore_
@sandhyarathore_ 10 ай бұрын
😂😂
@Dr..........
@Dr.......... Ай бұрын
Great comment😂❤
@Cardi-a
@Cardi-a 28 күн бұрын
Kitna phek rha h 😏
@madhuribegum5202
@madhuribegum5202 3 жыл бұрын
Sir i Need ur autograph 😊, i feel so happy watching ur videos. Thank you❤
@singhankul6794
@singhankul6794 2 жыл бұрын
Hii ji
@mbbs2002
@mbbs2002 Жыл бұрын
Come to government medical College Jalgaon Maharashtra,
@niteshrathor61
@niteshrathor61 Жыл бұрын
Padho padho Bakchodi nhi
@DrPooja-dp4pd
@DrPooja-dp4pd Жыл бұрын
@@niteshrathor61 😂😂
@74.bhaskerpodila90
@74.bhaskerpodila90 Жыл бұрын
bzsker...tqs .it's my autograph
@sk-kz4sz
@sk-kz4sz 9 ай бұрын
Biochemistry paper 2 tomorrow🔪🥹
@pyn0105
@pyn0105 9 ай бұрын
Same 😭😩
@shivanikadam7389
@shivanikadam7389 9 күн бұрын
And mine ut biochemistry paper 2 on 16 th of August
@shekharchoudhary3680
@shekharchoudhary3680 9 күн бұрын
Mine also​@@shivanikadam7389
@ashish2003-l8w
@ashish2003-l8w 8 күн бұрын
​@@shivanikadam7389maharashtra??
@ashish2003-l8w
@ashish2003-l8w 8 күн бұрын
​@@shivanikadam7389Maharashtra??
@navaneeth_us
@navaneeth_us 3 жыл бұрын
Top-notch conceptual correlation and beautifully crafted and articulated session. Thank you so much, Sir.
@s.r0700
@s.r0700 3 жыл бұрын
Big thank u sir🌹💗 Many of us requested u for this topic and we know that u will be there for us as always... We are also here🙏🙏🙏🙏🙏
@prachijha8264
@prachijha8264 Жыл бұрын
.I was doing rtpcr as a microbiologist since 1 month but didn't understand the process..now I get it.. Thanku sir
@ramakantarana2656
@ramakantarana2656 3 жыл бұрын
Excellent sir u simply represented the wet lab techniques for us ...lots of respect for this mol bio lecturer
@ankit123aa
@ankit123aa Ай бұрын
Mandal aaple aabhari ahe♥️ , dhanyawad sir🙏 , watching from Sambhaji nagar 🫡
@rahul554645
@rahul554645 3 жыл бұрын
Sir please make a video on glucose tolerance test as are exams are approaching and since you are the best. Thankyou so much for your work. ❤️
@gayathrigayu6228
@gayathrigayu6228 3 жыл бұрын
Really a super explanation sir......you make me more interested towards the subject and I don't like chemistry in 12th std but now biochemistry is a major subject .....after listening to your videos apart from micro and patho .. biochemistry is my favourite one and help to get more marks and understanding the concepts easily
@gouthamreddy2715
@gouthamreddy2715 10 ай бұрын
Which college are you from
@venkatesanmunusamy7319
@venkatesanmunusamy7319 3 жыл бұрын
Intensity of fluorescence increases with increase in amplification
@madnihassan
@madnihassan 2 жыл бұрын
Thnx sir love from Pakistan 🇵🇰
@Raja_YT23
@Raja_YT23 Жыл бұрын
This is really amazing video. It helps me a lot to understand the PCR. THANK YOU SO MUCH SIR.. ❤️
@anshuls8861
@anshuls8861 11 ай бұрын
jsr
@khushimaurya3670
@khushimaurya3670 3 жыл бұрын
Very well explanation sir clearly understood by anyone your way of explanation is so clear and smooth ... Thank u sir 🌼
@misthisinha3157
@misthisinha3157 2 жыл бұрын
Thank you very much sir 🙏.You are the best teacher of biochemistry
@kuldeepchouhan699
@kuldeepchouhan699 3 жыл бұрын
Thank u sir🙏🏻🙏🏻🙏🏻 SARS COV2 ka que hmari PU exam m abhi aya tha Thanks sir ab sb clear ho gya🙌🏻👐🏻
@dr.jamsheedjavid4455
@dr.jamsheedjavid4455 3 жыл бұрын
Nice explanation Dr. We hope in future we will be able to watch your lectures on some other important topics like DNA sequencing-sanger and NGS, microarray technology etc.
@kunalrawal4979
@kunalrawal4979 3 жыл бұрын
Thank u sir for making video on this topic , I was suggesting this video for very long . Hope you will continue this good work 🙏
@Priyanka95911
@Priyanka95911 3 жыл бұрын
Thank you sir for such a beautiful explanation of lab technique....
@umeshagrawal9417
@umeshagrawal9417 3 жыл бұрын
Sir can you please continue with the videos of important topics for University examination specifically Genetics 🙏
@namratashirwadkar6565
@namratashirwadkar6565 3 жыл бұрын
Excellent explanation 👍 Thank you sir
@jollygood592
@jollygood592 6 ай бұрын
You are only... who provided vaubale lectures more than paid lecture sir.... Thank you so much sir.. thanks alot ❤❤❤
@kudikamaal2840
@kudikamaal2840 3 жыл бұрын
Uh are few days late else this cud be helpful for my xam😅😅btw Always great explanation Sir ....keep up the good work ....☺️
@kudikamaal2840
@kudikamaal2840 3 жыл бұрын
@@dr.derbyallen7063 😶😶sorry sir 🤣😂
@navyasavant5107
@navyasavant5107 3 жыл бұрын
Nice explanation sir thank you
@rajalekshmijr149
@rajalekshmijr149 3 жыл бұрын
Thanku sir for this clear presentation
@glowupwithnature145
@glowupwithnature145 2 ай бұрын
nyc explanation but one thing I would like to tell that is taq polymerase don't do endonuclease activity (non proofreading)
@zebasiddiqui6622
@zebasiddiqui6622 3 жыл бұрын
Thank you so much sir... You made this topic so easy.. wonderful explanation..👍
@s.r0700
@s.r0700 3 жыл бұрын
Sir ab dna sequencing aur dna chips,vntr,rlfp etc ke upar bhi video banana pls
@amanofcultur9903
@amanofcultur9903 3 жыл бұрын
Thank you sir, can you make videos on electrophoresis and ELISA too?
@spshivani6828
@spshivani6828 3 жыл бұрын
Amazing 🙏 sir 🙏🙏🙏🙏👍👍👍👍👍
@abideen.k.pkondotty6410
@abideen.k.pkondotty6410 3 жыл бұрын
thank u very much sir ,you are an excellent teacher
@mudavathshankar4064
@mudavathshankar4064 11 ай бұрын
Thank you very much sir🙂
@Vaguspostedat
@Vaguspostedat 3 жыл бұрын
Thank you sir better than reading a book
@abhishekmishra8215
@abhishekmishra8215 Жыл бұрын
Sir please upload video on blotting techniques,gene therapy, bioinformatics, arterial blood gas analysis
@dhkm_002
@dhkm_002 Жыл бұрын
Thank u sirji
@nibeditasarkar6591
@nibeditasarkar6591 3 жыл бұрын
Thank you so much sir. Also for extra knowledge
@bathularamadevi3970
@bathularamadevi3970 3 жыл бұрын
Thank u sir... .simply explained
@shubhamaggarwal3801
@shubhamaggarwal3801 3 жыл бұрын
Thank you sir👍🏻☺️
@JUSTFun-fo7sn
@JUSTFun-fo7sn Жыл бұрын
Excellent
@RAMBABU-wx4ee
@RAMBABU-wx4ee 3 жыл бұрын
Sir please cancer and HIV topic
@MedicoPhillic
@MedicoPhillic 2 жыл бұрын
Sir if you want to earn a little more through these beautiful videos then please use yt studio in laptop or mobile chrome browser(desktop mode) and add 4-8 mid roll ads there and also thank you sir for making biochy interesting 🙏
@citydiagnostic5517
@citydiagnostic5517 3 жыл бұрын
Thank you very much sir For brief review
@fahadpasha8857
@fahadpasha8857 3 жыл бұрын
Loads of respect sir😊
@oshinibandara7129
@oshinibandara7129 2 жыл бұрын
Thank you for the great explanation.
@urvashishinghania9313
@urvashishinghania9313 3 жыл бұрын
You are really a nice teacher sir Thank u 🤗🤗
@drfaria2000
@drfaria2000 3 жыл бұрын
Welcome back sir....🤩🤩🤩
@RAMBABU-wx4ee
@RAMBABU-wx4ee 3 жыл бұрын
Thanks sir 🙏🙏
@rockandshineeditings9547
@rockandshineeditings9547 2 жыл бұрын
Sir next videos lo blotting techniques topics explain cheyyandi sir
@prakashchander9263
@prakashchander9263 3 жыл бұрын
Thank you .
@malikhussnain8374
@malikhussnain8374 3 ай бұрын
you are great sir g ,,,,, love for you muah
@icshahid
@icshahid 3 жыл бұрын
Love sir ❤️
@Sunshine-ct3zq
@Sunshine-ct3zq Жыл бұрын
Much respect to you sir🙏
@Noor-ty1xt
@Noor-ty1xt Жыл бұрын
Pct requirements 1)template strand 2) primer 3)taqpolymeras 4)dntps Pcr machine
@drsachin246
@drsachin246 3 жыл бұрын
Thanks.
@RohitGurjar-ny7zn
@RohitGurjar-ny7zn Жыл бұрын
Nice sir
@kishmadevi865
@kishmadevi865 3 жыл бұрын
Thank you so much sir
@kumar7sachin
@kumar7sachin 3 жыл бұрын
Sir please make new vedio on molecular genetics old video sound not clear
@deviprasanna5694
@deviprasanna5694 3 жыл бұрын
Sir please explain biosynthesis of triacylglyserides
@mohitsharma-vo5gi
@mohitsharma-vo5gi 3 жыл бұрын
Thanks a lot sir 🥰🥰
@bhuvneshwarivala5326
@bhuvneshwarivala5326 3 жыл бұрын
Sir please give the imp topic in extracelluar matrix and tissu Protein please sir and also water and electrolyte balance thank you sir
@theabhigautam
@theabhigautam 3 жыл бұрын
Sir, if we take one DNA molecule and attach the Taqman probe to one strand, then how the probe will increase its fluorescence, as DNA will replicate and produces multiple copies.
@belexhanns6024
@belexhanns6024 Жыл бұрын
Simplified PCR thanks
@mitthunishad
@mitthunishad 8 ай бұрын
Hats off to ur way of teaching..
@nycboxing8359
@nycboxing8359 2 жыл бұрын
Keep it simple!
@legolas-yj7mp
@legolas-yj7mp 9 ай бұрын
From knruhs 🙋🏻‍♂️..!
@priyanshijoshi8321
@priyanshijoshi8321 2 жыл бұрын
Thank you sir
@uzairqureshi3605
@uzairqureshi3605 2 жыл бұрын
sir thankyou!
@niloferishrath2461
@niloferishrath2461 3 жыл бұрын
sir can you take the next topic on" meister cycle "?
@devendrasrivastva3610
@devendrasrivastva3610 3 жыл бұрын
PCR thermocycler can not be used after RT is done manually for quantitative of COVID
@Doctor__22
@Doctor__22 10 ай бұрын
Thank you so much sir ❤️
@sachinrao4089
@sachinrao4089 3 жыл бұрын
👍🏿👍🏿sir
@Innervoice_Sukoon
@Innervoice_Sukoon 2 жыл бұрын
How can i explain real time pcr in layman language by giving real life example
@ragnarok9197
@ragnarok9197 2 жыл бұрын
🔥🔥🔥
@souravmohanty1200
@souravmohanty1200 Жыл бұрын
Thanks sir 🙂🙏
@Abhishek-fe7hc
@Abhishek-fe7hc 3 жыл бұрын
👌👌👌
@sohanyadav5272
@sohanyadav5272 2 жыл бұрын
Thank you sir 🙏🙏🙏🙏🙏
@alagumani6559
@alagumani6559 3 жыл бұрын
Sir pls start msc biochemistry online course
@mokallakomuraiah3747
@mokallakomuraiah3747 3 жыл бұрын
U didn't tell us at which prime ,
@medmedicose7490
@medmedicose7490 3 жыл бұрын
❤️👌🏻
@prajwalareddy2603
@prajwalareddy2603 3 жыл бұрын
Thank u so much Sir
@chengalvavishwa5879
@chengalvavishwa5879 3 жыл бұрын
What is the difference between real time PCR and reverse transcriptase pcr
@Rajesh_Jambhulkar
@Rajesh_Jambhulkar 3 жыл бұрын
Reverse transcriptase PCR can be real time, real time PCR is live tracking of amplification
@chengalvavishwa5879
@chengalvavishwa5879 3 жыл бұрын
@@Rajesh_Jambhulkar Thank you sir
@nitintyagi6009
@nitintyagi6009 3 жыл бұрын
🌹🌹🌹🌹
@rks.
@rks. 3 жыл бұрын
After 30 cycle it produces billion copies not million
@ayoubkhan6563
@ayoubkhan6563 2 жыл бұрын
Ruta master
@GaganNarangOfficial
@GaganNarangOfficial 3 жыл бұрын
Sir please tell me a simple words ....rt-PCR and PCR are same or not...please reply sir ji....
@FF-zv5ho
@FF-zv5ho 3 жыл бұрын
Other virus can be there also in swab How it detect it as covid?
@saptarshibhattacharya
@saptarshibhattacharya 4 ай бұрын
By reverse transcription, that is by converting the viral RNA to DNA and then applying PCR
@Thecaveandcognitionmusic
@Thecaveandcognitionmusic 3 жыл бұрын
so then, are all pcr tests for covid 19 the same?????
@user-bx1xk8gl5s
@user-bx1xk8gl5s 11 ай бұрын
👏👏👏
@medicaleducation8978
@medicaleducation8978 2 жыл бұрын
Sir in reverse transcriptase PCR we are not using detectors or not ?
@wolfgangflywheel4307
@wolfgangflywheel4307 3 жыл бұрын
RT-PCR is a manufacturing process NOT a diagnostic tool. RT-PCR amplifies the target so that one can do further study; it does not and cannot distinguish between something that is actively infective or not infective.
@amiteshvel3791
@amiteshvel3791 3 жыл бұрын
First view 👍🏻
@MAni-wm1lc
@MAni-wm1lc 2 жыл бұрын
Can I travel with pcr test instead of rt pcr
@Khanak2017
@Khanak2017 6 ай бұрын
Sir please upload types of pcr
@ambikasaxena3550
@ambikasaxena3550 2 жыл бұрын
Can you please describe more about the chemistry of pcr or mention the book or site where we can find about it
@jeni3486
@jeni3486 9 ай бұрын
🙌🏼
@dtapabcd390
@dtapabcd390 3 жыл бұрын
14:10 RTqPCR
@bushrakhan-2248
@bushrakhan-2248 6 ай бұрын
annealing required below 5 degree/
@angelasusan8629
@angelasusan8629 8 ай бұрын
bad
@zameerrahi9039
@zameerrahi9039 3 жыл бұрын
ads ki wjs sy dislike krna pra
@sajadahanger3087
@sajadahanger3087 2 жыл бұрын
I wasted my time by watching this video
Lac operon with animations
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