Plant Tissue Culture - Fern spores tissue culture (Bird's nest fern)

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Plant Tissue Culture - Fern spores tissue culture (Bird's nest fern)

Рет қаралды 4,459

Күн бұрын

Ferns are epiphytes and are widely cultivated for ornamental purposes. It is undoubtedly a great choice for a house plant.
The spores are often found underneath the leaves, which are protected in a capsule called sorus.
Such propagation method is much easier to be grown outside a lab as the spores are powdery to be disinfected via surface sterilization.
A successful plant tissue culture protocol was conducted and there was no significant difference between the surfactants using Plant Preservative Mixture, PPM and Clorox.
A germinated spore produced a structure called prothallus, which is a love-shape structure that is green in colour.

Пікірлер: 45

@edberry4904 3 жыл бұрын

Please turn that horrible music and tell us what materials you are using. Otherwise the video is good but useless. Respectfully

@oshiperera8009 3 жыл бұрын

Yeah thats true

@iajjsjjsjsjsjdjwuw 2 жыл бұрын

indeed

@mahmoudsayeh9169 3 жыл бұрын

Fantastic

@guiovannivivas4270 Жыл бұрын

Si aportas al conocimiento e iniciativa agrícola. Leo comentarios impertinentes, hacer caso omiso. Si pudieras incluir mas info tecnica a este vdo concentraciones, materiales, tiempos, etc seria mas refull. Agradezco su compartir. From Colombia sud america Guiovanni, Are you Ucrania? Whwwre is?Thanyou

@Plantastic12345 Жыл бұрын

I am from Malaysia. Thank you for being so supportive. I am trying to make more content in the future.

@jedz86 4 ай бұрын

is this last medium with nutrients? i mean water in last jar u thrown spores in? u used ppm vs chlorox ?

@Plantastic12345 4 ай бұрын

Can you state the time in the comment?

@jedz86 4 ай бұрын

@@Plantastic12345 27:24 media on petri which media u using? just ms media ?

@Plantastic12345 4 ай бұрын

@@jedz86 yes, it’s MS basal media. At this stage, we usually don’t add any hormones as there might be contamination. Moreover, the growth is super fast once it’s germinated without contamination.

@InTheSkyWithLucie Жыл бұрын

Hi im trying to replicate your methods but some details arent clear. Based on the comments I've understood that you used 1-10% or 5-10% clorox solutions for 30 minutes and one rinse with distilled water before culturing on the plates. Unclear details are: What is the concentration of PPM used ? do you add it only to the first sterilizing solution or do you also add it to the culture medium? How long did you have them running under the tap water? Did you have them in the same tap water when u started in the bench? Did you go straight from the tap water to the clorox solution? After the clorox solution, did you only rinse the spores once with sterile water? or 3 times like most tissue culture protocols? and just curious what was your contamination rate? I tried it already twice and i have almost 100% contamination. Any help is appreciated thanks.

@Plantastic12345 Жыл бұрын

1. PPM is strictly depends on the conditions of the explants (spores in this case). You may use 5, 10, 15, and 20 mL per liter in the gelled media as I’m not sure if the growing condition of the plant bearing spore is “clean” or not. 2. More than an hour. 3. Yes, same. 4. Quick dip in 70% ethanol before Clorox. The concentration of Clorox is strongly dependent on the amount of the spores per tea bag and the penetration of the tea bag. 5. All sterilization process in my videos are: Tap water> 70% ethanol> Clorox> rinsing with sterile water. The reason I don’t put concentration used and duration is because everything has to be adjusted based on the explants we used and the status of the explants.

@InTheSkyWithLucie Жыл бұрын

@@Plantastic12345 Thank you! I'll try it again and let you know results in 1-2 months. Ive already noticed few of the spores from previous experiment is green now :) thanks

@gabewalker9170 3 жыл бұрын

That was great thankyou! Can you tell me what solution the spores were pipetted into the Petri? It looked like it set in the dish or was there medium in the dishes already?

@Plantastic12345 3 жыл бұрын

It was the sterile distilled water from the rinsing part of the sterilization..

@gabewalker9170 3 жыл бұрын

In the last slides of the grown out Petri dishes, have they got ms/agar solution media and the explants have been transferred to these or are they just grown out to the size of the last slides in the water?

@Plantastic12345 3 жыл бұрын

@@gabewalker9170 they were germinated since the day I incubated them on the SAME medium...with the water of course

@gabewalker9170 3 жыл бұрын

So there was ms/agar in in the Petri dishes before placing the spores in the sterile h20 into the dishes?

@gabewalker9170 3 жыл бұрын

Sorry if I am confused. In the very last slide with most growth it looks like they are on agar? All the rest look like water.

@carlschulenburg9032 2 жыл бұрын

Hi. Im currently busy inoculating stag horn fern spores. I was wondering what percentage dilution of your disinfectant did you use? How long did you leave the spores in the disinfectant? What yellow liquid did you add before you disinfested the spores? What agar media did you use? Love the video!

@Plantastic12345 2 жыл бұрын

5-10% bleach solution... The duration varies according to the amount of spores used per batch... Roughly 30-60 mins

@carlschulenburg9032 2 жыл бұрын

@@Plantastic12345 thank you. I don’t have that many spores like you. About a quarter of that. how many rinse steps did you do? What pipet did you use? What agar did you use? Sorry for all the questions.

@Plantastic12345 2 жыл бұрын

@@carlschulenburg9032 normal medium is fine...I used MS medium without any PGR for all videos...rinsing can be many times depends on your trials... Pipette of any brands is the same...just that you need to autoclave the tips before you use them...

@hholi 9 ай бұрын

@@Plantastic12345 do you think only the ms medium will work for tissue culture of lets say p.superbum ?

@Plantastic12345 9 ай бұрын

@@hholi Hi, actually every medium works, just that MS medium is the most easily available and ready for the stock preparation…there are many cases where you can see people adjust the macronutrient as MS is well-known as high salt concentrations…

@chloev9275 2 жыл бұрын

what % clorox solution did you use?

@cloudyrainbow6806 2 жыл бұрын

How many months do the spores take to germinate? I tried to germinate mine, but all I got is some tiny white fuzy colony but its only been one month. Confident not fungus since it didn't spread to the whole petri plates. Please help. Thank you!

@Plantastic12345 2 жыл бұрын

You may need to add some drops of water to make sure they are fertilized…whole process would take about 1-2 months depends on the species

@cloudyrainbow6806 2 жыл бұрын

@@Plantastic12345 i see. Thanks for your advice! Is it common that germinated spores are white in colour, will they turn green by time?

@Plantastic12345 2 жыл бұрын

@@cloudyrainbow6806 I tried only one species in which they are green upon the germination

@AquaMayne 2 жыл бұрын

Hello what did you use to sterilize the spores?

@Plantastic12345 2 жыл бұрын

Diluted Clorox at 1-10%

@AquaMayne 2 жыл бұрын

@@Plantastic12345 Then rinse with sterilized water?

@Plantastic12345 2 жыл бұрын

@@AquaMayne yes

@stuartxu8069 Жыл бұрын

are the plants grown from such a technique still clone to the original plants? or they went thru some sort of sexual reproduction phrases?

@Plantastic12345 Жыл бұрын

You need some moisture to make sure it’s fertilized between individuals… I didn’t proceed with further stage as this is the stage that client wants as they mentioned that induction of thallus phase in nature is very slow

@handaruaditomo6887 3 жыл бұрын

this is good scientific video but edited like 12years old trying to be viral. just talk and explain. it may sounds plain and boring but admit it no kids gonna watch your video. we are all plain and bored adults.

@Plantastic12345 3 жыл бұрын

That's great idea, but all of the videos are not made for kids... They got to understand very basic of science prior to advance technology like this....

@handaruaditomo6887 3 жыл бұрын

@@Plantastic12345 thats exactly what i said. your topics is not made for kids but the way you edit it is like for kids. come on man stop using such music and stop the obnoxious subscribe begging

@Plantastic12345 3 жыл бұрын

@@handaruaditomo6887 thanks for telling...if anything damaged your eyes and mind, please feel free to block my channel and share my stupid way of editing it... Please contribute instead of insulting...thanks!

@mustafakahya1209 3 жыл бұрын

You should not use gloves while burning with alcohol in the cabinet. It may have bad consequences ..!

@Plantastic12345 3 жыл бұрын

Thanks for reminding, but, alcohol itself is a dehydrating agent...it will dehydrate your hands...one must be always aware of the alcohol on the gloves before it dried up...I've been wearing gloves for 5 years working on tissue culture and it's not an issue for me...for beginner, one must have extra precautions on it...

@mustafakahya1209 3 жыл бұрын

@@Plantastic12345 Very good videos. I will have my students watch it in the lesson. Achievements.

@Plantastic12345 3 жыл бұрын

@@mustafakahya1209 thank you!