Polyclonal antibody

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This lecture explains about polyclonal antibody structure and the production of polyclonal antibodies. It also explains the use of polyclonal antibody in biological techniques like immunobloting.
Polyclonal antibody lecture - In this video lecture Suman Bhattacharjee shares information about the principles of Polyclonal antibody. It also explains the role of Polyclonal antibody. Watch this lecture to know more details about the Polyclonal antibody.
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Thank you for watching

Пікірлер: 94

@pritiswain3718 5 жыл бұрын

Polyclonal are those when serum contains different ab against single antigen for different epitope and each one is specific for one epitope. I think you might have told unconsciously that one ab recognise different epitope.

@michaelleischnig6435 4 жыл бұрын

I read this remark like 2,3 times here (@MegaMie77, @spandita roy), so for clarification, as I understand it: If you inject a specimen A (let's say a mouse) with a specific antigen, this host will maturate lymphatic B-cells into B-plasma cells (through the known pathway macrophages - dentritic cells - lymphatic T-cells - T-helper cells) that produce antibodies against it. These Ab can be extracted as a serum. Normally, an antigen has more than one epitope with which an antibody can interact. Therefore, the extracted serum/mixture would contain different antibodies against the antigen, (as a whole) so-called polyclonal antibodies. Still, each of the antibodies can only interact with one specific epitope expressed on said antigen. Hope that helps/is at least accurate :D

@mayukhroy5393 4 жыл бұрын

Thank you Sir, this video was helpful

@rinaevasoamamy8493 8 жыл бұрын

thank you for the explanation

@fundamentalzoology7131 3 жыл бұрын

Nice lecture sir. Easy to understand.

@Thats_my_tale 2 жыл бұрын

U explain really great I have 10 days for my enterence exam and with your videos i have completed so many things in less time and u explain in such a simple way tht it's really easy to remember

@shomusbiologyofficial 2 жыл бұрын

Thank you so much for appreciating my efforts

@spanditaroy9973 5 жыл бұрын

Polyclonal antibody is actually collection of antibodies produced from different B cell lineages that's why they can interact with different epitopes of same antigen. Am I right?

@pritiswain3718 5 жыл бұрын

Yes

@vijayag1957 3 жыл бұрын

Yes

@tusiye4356 Жыл бұрын

you are really simplifier, and recently detected your Chanel accidentally, and it's now one of my first sources for referring when things go tough in biology . Thanks

@shomusbiologyofficial Жыл бұрын

Thank you so much for appreciating

@sydneysimukoko Жыл бұрын

Indeed the man is very great, I've been watching his videos since my 1st year, I'm now in 3rd year doing Veterinary Medicine at the University of Zambia 🇿🇲.

@godslave85 4 жыл бұрын

thank you very much

@anyannoaxom6290 4 жыл бұрын

Your insight is now becomes applicable to understand Antigen against covid 19. Thanks for your anticipation years back

@shomusbiologyofficial 4 жыл бұрын

Thank you. Glad you liked my lectures

@bhargavkbh5739 2 жыл бұрын

Specificity and affinity these are the unique words used in this video ❤️

@shomusbiologyofficial 2 жыл бұрын

Thank you. Glad to hear that you are getting benefit from the lectures

@rahilabbas643 2 жыл бұрын

Love the way... You explained sir... Hats off Sir jee

@shomusbiologyofficial 2 жыл бұрын

Thank you so much for appreciating my efforts

@maryamzulfiqar9390 2 жыл бұрын

u helped me teach better understanding of immunity and vaccines to my students.. thank u

@shomusbiologyofficial 2 жыл бұрын

You're welcome. Glad you like it

@Talentcreationndimagination 5 жыл бұрын

Sir u are really the great . If I will meet u then I will do the robbbery of ur mind😎

@shomusbiologyofficial 5 жыл бұрын

Ha ha

@jyoti2298 4 жыл бұрын

Thank you sir for such a informational vedio .......❤️

@shomusbiologyofficial 4 жыл бұрын

You're welcome. Glad to hear that you're getting benefit from my lectures

@farheenbegum6784 5 жыл бұрын

nice...explaination.and thank u...

@shomusbiologyofficial 5 жыл бұрын

You're welcome

@ssubbiahsubramanian4353 4 жыл бұрын

Sir is plasma cell and mast cells are one and the same

@Semsempharma1 5 жыл бұрын

God bless you , you explain in very simple and clear

@shomusbiologyofficial 5 жыл бұрын

Thank you. Glad you liked my lectures

@soumyaranjanbarik923 3 жыл бұрын

Thank u so much sir... I got complete idea about it....

@shomusbiologyofficial 3 жыл бұрын

You're welcome. Glad to hear that you're getting benefit from my lectures

@manasasanikere91 4 жыл бұрын

Sir could you please make one vidio about photo bioreactor

@triptyayesha8845 5 жыл бұрын

Please make a video on antibody humanization

@rajyalakshmi902 3 жыл бұрын

Sir u made many scientists lecturers 🙏

@shomusbiologyofficial 3 жыл бұрын

Thank you

@alexandraandronik7479 Жыл бұрын

Thank you!!!!!!!❤

@shomusbiologyofficial Жыл бұрын

You're welcome

@zaheeriqbal3377 2 жыл бұрын

Ur great sir g

@shomusbiologyofficial 2 жыл бұрын

Thank you

@Dr_Syed_Ali_Rukh 3 жыл бұрын

Shabash

@sadodexter4228 5 жыл бұрын

Excellent work

@shomusbiologyofficial 5 жыл бұрын

Thank you

@mittukoshy1986 6 жыл бұрын

Excellent way of teaching. Even a person from non biology background also can understand this well.

@kopiyofred2619 8 жыл бұрын

Thanks for your clarification,

@swetambari95 3 жыл бұрын

Very informative 😊

@shomusbiologyofficial 3 жыл бұрын

You're welcome. Glad to hear that you're getting benefit from my lectures

@MegaMie77 5 жыл бұрын

You have the general idea right but off when you get into specifics. For a start, there is no such thing as a polyclonal antibody. It would be synonymous to saying someone has multiple birth mothers! Impossible right?! Polyclonal is the mixture of antibodies from multiple cells before clone selection. Yes, the mixture will target different epitopes but on a micro level, each antibody can only be specific to a single epitope.

@qigong1001 3 жыл бұрын

No, you can have cross reactivity.

@emamulhassan3955 2 жыл бұрын

So helpful❤️

@shomusbiologyofficial 2 жыл бұрын

Thank you

@ajuljose9064 4 жыл бұрын

Thank you

@shomusbiologyofficial 4 жыл бұрын

You're welcome

@tanzeemazeeza.s9589 8 жыл бұрын

your lectures are very useful sir thank u so much

@whiterose5206 7 жыл бұрын

thank you for your easy explanation. big help here

@timfitzgerald4079 7 жыл бұрын

Excellent presentation and explanation of mono/polyclonal antibodies..👍👍👍

@user-xe4nq1gt9l Жыл бұрын

Is only one B cell producing different types of antibodies?

@ullaskotihal1911 5 жыл бұрын

Thanks bhai

@shomusbiologyofficial 5 жыл бұрын

You're welcome

@ullaskotihal1911 5 жыл бұрын

We wanna meet you. Where are you from?

@obiwan922 5 жыл бұрын

You are saying, a polyclonal antibody is the one which can bind with different types of epitopes? 3:55 . I didnt get this part. (Maybe i learned wrong then!)

@fundamentalzoology7131 3 жыл бұрын

Different epitopes means different regions of the antibody, those make bond with antigen. For better understanding you must know the molecular structure of the antigens.

@layjain7097 5 жыл бұрын

Nice

@shomusbiologyofficial 5 жыл бұрын

You're welcome

@karnailsingh2629 4 жыл бұрын

Sir Again a short lecture about polyclonal and monoclonal Ab in hindi

@shomusbiologyofficial 4 жыл бұрын

Coming soon

@sunilpaudel6013 9 жыл бұрын

thank you Sir......Now i'm cleared about mono and polyclonal antybody........thanks

@wasimakram6892 5 жыл бұрын

Good

@shomusbiologyofficial 5 жыл бұрын

Thank you

@silpasumandebasharma273 2 жыл бұрын

Sir ..is there any book in which this topic is present

@shomusbiologyofficial 2 жыл бұрын

Kuby immunology

@silpasumandebasharma273 2 жыл бұрын

@@shomusbiologyofficial thank u ...and for SDS page which book ...?

@zakiasultana2986 4 жыл бұрын

Why we can't use a single antibody as monoclonal antibody from the polyclonal antibody mixture?

@rucha3428 8 жыл бұрын

thank you sir. i have a doubt. why antibody produced are type of IgG only?

@ullaskotihal1911 5 жыл бұрын

Fan from Karnataka 😍 We wanna meet you

@shomusbiologyofficial 5 жыл бұрын

You're welcome

@maryamamer5295 5 жыл бұрын

❤❤👏

@shomusbiologyofficial 5 жыл бұрын

You're welcome

@sarinasarina3973 7 жыл бұрын

thank you、 sir。how to choose the primary and secondary antibody？

@waitspankaj 7 жыл бұрын

thank you so much.. sir..can u post a video on nano technology and tissue engineering in medicine..

@shomusbiologyofficial 7 жыл бұрын

+pankaj k I will try to post videos on that topic. Thank you

@beenaasokan8569 7 жыл бұрын

sir can you post a video on vaccines and classification

@shomusbiologyofficial 7 жыл бұрын

+beena. r it's already there. You can find it 8n my channel

@bublymallik8930 6 жыл бұрын

The audio of this vdo is not good...but the lecture is good 👍

@siprachauhan1958 2 жыл бұрын

Hindi me samjhaye sir g jayada samjh aayega or like v milega

@shomusbiologyofficial 2 жыл бұрын

Like nehi chahiye

@aashikas3859 3 жыл бұрын

Sir if we use tumor cell for multiplication is there any chance tumor formation

@AnkushSharma-zv5hv 7 жыл бұрын

actually polyclonal antibodies are mixture of monoclonal antibodies....which bind to specific epitope but since they are mixture...so they bind to various specific epitopes.....