Primer design |in silico cloning | SnapGene

Primer design |in silico cloning | SnapGene | UCSC Genome browser

Рет қаралды 11,937

Күн бұрын

This video lecture explains
1. How to use UCSC genome browser to extract different regions of the gene of interest?
2. How to design the primers to amplify the region of interest using SnapGene?
3. How to design primers for cloning the insert ?
4. How to check the size of the PCR product flanked by the designed primers?
5. How to use Snapgene?
6. How to perform in silico cloning in snapgene?
7. How to select the restriction enzymes?
8. How to design primers with restriction enzyme site?
#cloning #biologylectures #genetics
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Пікірлер: 38

@AmruMagdy 3 ай бұрын

Thanks also teacher, God blessed you

@BiologyLectures 2 ай бұрын

You are most welcome

@gutsandglory7507 Жыл бұрын

Guy you have nailed it. Thank you so much for this wonderful video. Straightforward and clear crystal. Greetings from Russia

@BiologyLectures Жыл бұрын

You are very welcome

@gutsandglory7507 Жыл бұрын

Thanks once more, I tried all the steps (with 3-4 deviations) using my assignment project. It was a success. I am glad to find this tutorial on my time of need.

@BiologyLectures Жыл бұрын

Very glad to hear that this video helped with your assignment. Please like and share and subscribe to our channel to support us.

@mishralab2221 2 жыл бұрын

Fantastic job explaining this! Thank you.

@BiologyLectures 2 жыл бұрын

Thank you very much.

@muskansyed2037 Жыл бұрын

Just the thing I needed to observe carefully before kickstarting on my first cloning experiment. Wonderfully described aspects of cloning by you Sir. Hats off

@BiologyLectures Жыл бұрын

Thank you very much for the kind words. Please like and share the video and subscribe us to support us.

@ericwuluglayjr6060 11 ай бұрын

Thanks also teacher, God blessed you 🍒

@BiologyLectures 11 ай бұрын

Thank you very much. God bless you too

@user-tz8sf1dm8c 8 ай бұрын

very useful just saw this before my practical ... very well explained

@BiologyLectures 8 ай бұрын

Glad you liked it

@shrilaxmibhat9713 2 жыл бұрын

Thank you for the video.Excellent explanation given, 😊wish to see more such videos in future.

@BiologyLectures 2 жыл бұрын

So nice of you. You are most welcome. Please subscribe us to support us.

@negessemekonnen1854 Жыл бұрын

very important demonstration, thanks!

@BiologyLectures Жыл бұрын

You are most welcome 🤗

@Hari788 3 жыл бұрын

Excellent work. Thanks .

@BiologyLectures 3 жыл бұрын

Thank you very much. Please consider subscribing us for more contents like this.

@magdaescobar3697 2 жыл бұрын

Excellent video. You helped me a lot with your explanation

@BiologyLectures 2 жыл бұрын

Glad it helped! Please subscribe our channel to support us.

@dikshyapanthi7681 3 жыл бұрын

Excellent explanation I was looking for something like this

@BiologyLectures 3 жыл бұрын

Thank you very much. Please consider subscribing us for more contents like this.

@muhammadichsan2558 3 жыл бұрын

Very nice explanation.. Keep going

@BiologyLectures 3 жыл бұрын

Thank you very much:-)

@SanjaySingh-oe1ts 3 жыл бұрын

Good Explaination. thanks

@BiologyLectures 3 жыл бұрын

Thank you. Please share the video and subscribe our channel for more contents like this.

@sadullaabdullaev5451 2 жыл бұрын

Very useful and excellent work, it would be great if you demonstrate how to use Ensembl plants !!!! thank!

@BiologyLectures 2 жыл бұрын

Thank you, we will certainly make a video on Ensembl plants.

@ogunoluwamayowa4749 3 жыл бұрын

Amazing explanation. Can I also just design cloning targeting just one exon?. I mean like you just did for 3' UTR. Just coping the targeted exon sequence

@BiologyLectures 3 жыл бұрын

You are most welcome. Yes you can use just one exon or any other part of the gene to do cloning.

@ogunoluwamayowa4749 3 жыл бұрын

@@BiologyLectures Thank you. I subscribed to your channel

@BiologyLectures 3 жыл бұрын

@@ogunoluwamayowa4749 You are most welcome and thank you very much for the subscription. Please share the video also.

@sreeram6416 4 ай бұрын

Sir I'm given a task of cloning the cDNA sequences of all the rna binding proteins in a vector called pEGFPN-3X-HA, which contains the HA tag. I retrieved the cDNA sequences of all the RBP's using Linux commands in a single go, and now I want to clone the genes into the pEGFPN-3X-HA such that these RBP after they are cloned and expressed will have HA tag in it and I can pull it using anti-HA antibody. My doubt is could you suggest me any software or tool in which we can synthesize primers in one go for these RBP such that they have respective restriction sites in them and they express the HA tag also

@deejaywonderer9814 3 жыл бұрын

where can i download the software you are using here?

@BiologyLectures 3 жыл бұрын

PLEASE FIND THE LINK HERE www.snapgene.com/vector-nti?gclid=CjwKCAjwr56IBhAvEiwA1fuqGgewE9pWgM2Gns0Z0rB48r4wg8U882APQYTZ9yVCjNjaW2epxaDYhhoCDa4QAvD_BwE