Competitive ELISA Tutorial 2: How to Use Calbiotech's Competitive ELISA Kits

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Here, we illustrate how to perform a typical Competitive ELISA (Enzyme-Linked Immunosorbent Assay) using a commercial kit from Calbiotech. In addition to demonstrating each step, we provide a few important tips that may help the scientist achieve good results with minimal troubleshooting.
Our ELISA tutorials are kindly sponsored by Calbiotech. Click here to see Calbiotech's ELISA kits: protocol-place.com/Calbiotech
Be sure to watch our other ELISA tutorials to learn more about this valuable technique. Here are all of the videos in this series:
ELISA Tutorial 1: Understand How an ELISA Works - • ELISA Tutorial 1: How ...
ELISA Tutorial 2: Coating and Blocking the ELISA Plate - • ELISA Tutorial 2: Coat...
ELISA Tutorial 3: Preparing and Adding Samples to the ELISA Plate - • ELISA Tutorial 3: Prep...
ELISA Tutorial 4: Finishing the Assay (Sandwich ELISA) - • ELISA Tutorial 4: Fini...
ELISA Tutorial 5: Preparing ELISA Data in Excel for Analysis with GraphPad Prism - • ELISA Tutorial 5: Prep...
ELISA Tutorial 6: How to Analyze ELISA Data with GraphPad Prism - • ELISA Tutorial 6: How ...
Competitive ELISA Tutorial 1: How a Competitive ELISA Works - • Competitive ELISA Tuto...
Competitive ELISA Tutorial 2: How to Use Calbiotech's Competitive ELISA Kits - • Competitive ELISA Tuto...
Competitive ELISA Tutorial 3: Analyzing Typical ELISA Data in Excel - • Competitive ELISA Tuto...
We hope you enjoy watching and benefit from our tutorials. If so, please take a minute to "like," or better yet, share them with others!
Thanks for watching!
Youssef Farhat
Protocol Place: protocol-place.com
**Check out some of our other tutorials via the links below**
Competitive ELISA Tutorials - - - • Competitive ELISA Tuto...
ELISA Tutorials - - - • ELISA Tutorial 1: How ...
Gelatin Zymography Tutorials - - - • Gelatin Zymography Tut...

Пікірлер: 15

@Reivivus 8 жыл бұрын

Man, this is so useful to see the experiment in real time with those animations!

@lyricanakin844 2 жыл бұрын

i know im asking the wrong place but does someone know a trick to log back into an Instagram account..? I was dumb forgot the password. I would love any tricks you can give me.

@roryfletcher1124 2 жыл бұрын

@Lyric Anakin instablaster :)

@lyricanakin844 2 жыл бұрын

@Rory Fletcher I really appreciate your reply. I got to the site thru google and im trying it out atm. Looks like it's gonna take a while so I will get back to you later with my results.

@lyricanakin844 2 жыл бұрын

@Rory Fletcher it worked and I actually got access to my account again. I'm so happy:D Thanks so much you saved my ass !

@roryfletcher1124 2 жыл бұрын

@Lyric Anakin Glad I could help xD

@mn7828 4 жыл бұрын

thanks for the video! so useful when learning the technique from scratch!

@drRunp 4 жыл бұрын

watching this as a student in 2020, so useful

@greencard1245 2 жыл бұрын

Thank you again Youssef !

@saadzah5930 3 жыл бұрын

Many thanks for your videos.

@mrsrekima5593 7 жыл бұрын

this is so helpful and useful thank you so much and god bless you

@drzenaAcademy 3 жыл бұрын

Great great vidio thanks

@romina222 9 жыл бұрын

Thanks a lot for the explanation! I want to ask, if a patient is infected with a virus then I should expect to see a yellow color, right?

@nwilczyn 7 жыл бұрын

the more present the infection in the sample, the less color present... it is the opposite of the result with a typical ELISA... because the color is coming about as a result of the enzyme conjugate binding to the antibodies. If there is more binding of conjugate (more color), it means there is less of the protein of interest in the sample. And then the opposite, if there is less color it means there is more of the protein of interest in the sample