This video will be discussing the quantitative western blotting workflow and how optimization is critical for achieving reproducible data. Presented by Dr. Sean Taylor.
Covered topics include:
0:00 - Overview of western blotting workflow
1:27 - What optimize?
2:40 - Optimization of sample preparation & electrophoresis running condition
7:14 - Optimization of protein load
14:42 - Optimization of blocking and antibody incubation
18:39 - Optimization of detection and quantitation
25:06 - Stain-free gel technology
26:31 - References
See products at www.bio-rad.com/westernblotting
We Are Bio-Rad.
Our mission: To provide useful, high-quality products and services that advance scientific discovery and improve healthcare. At Bio-Rad, we are united behind this effort. These two objectives are the driving force behind every decision we make, from developing innovative ideas to building global solutions that help solve our customers' greatest challenges.
Connect with Bio-Rad Online:
Website: www.bio-rad.com/
LinkedIn: / 1613. .
Facebook: / biorad
Twitter: / bioradlifesci
Instagram: @BioRadLabs
Snapchat: @BioRadLabs