There is a need for mass spectrometry based analytical workflows that facilitate rapid screening and quantitation of target analytes in biological fluids. In an ideal world, the same sample can be used for both screening and confirmation. However, in most cases, two independent samples and sample-prep workflows are needed to isolate the target analytes for the screening, quantitation and confirmation steps.
In this poster video, we present a technology that can be used to capture target compounds from diverse biofluid samples and perform rapid screening and quantitation via direct-to-MS experiments. In addition, the same device can be used for confirmation of said analytes via LC-MS/MS without further sample preparation. This novel technology, known as “coated blade spray” (CBS), provides reliable and reproducible results and with a total analysis time under 5 minutes for the rapid screening protocol.
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