RT- PCR, Reverse transcription PCR# RT- qPCR # Genes & Genus
Applications:
Reverse transcriptase PCR determines tissue-specific gene expression.
The present method is used to monitor the prognosis and therapy responses to cancer patients.
The amount of tissue-specific mutant alleles can be determined.
It is also used in gene insertion studies and gene therapy experiments.
Disease diagnosis and microbial identification can be done using this technique.
RT-PCR can measure viral load, expression, and infection.
Advantages of reverse transcription PCR:
The method can do quantitative as well as qualitative analysis.
It required a smaller amount of sample gene expression studies.
Post PCR processing such as agarose gel electrophoresis is not needed here.
The method is simple, easy to use, rapid and cost-effective.
Also, the specificity and the sensitivity of the reverse transcription PCR is enormous.
Disadvantages:
The method is extremely sensitive, even a small amount of DNA contamination can lead to false results.
The method is restricted for some of the assays as higher expertise and experimentation are required to develop new assays.
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