Sajid, is seriously, your explanation and visual methods was SO AMAZING, thanks a lot!!!!

I have seen many videos on this topic but no one explained like this! this is such a nice explanation!!😊

I missed a question about it in Uworld for USMLE step 1, and their explanation could not help me understand the concept, and you did. Thank you so very much!

Omg YOU EXPLAINED THIS SO WELL. I get it now.

Thank you, this helped a lot! Excellent work! I'm studying in german, but I could still understand everything very well (:

Very clear explanation! Thank you!

I'm very happy now, all my doubts on RIA are cleared, thank u so much sir..😊

I couldn't understand clearly in my class and was very tensed about it cause my exam is on thrusday.But your explanation gave me relief.very effective video......

Thank you so much ,saved at least an 2to 3 hours of time and energy....

Thanks a lot. This has helped a lot with my physiology course

Way to represent is excellent, covered all the major points in short video, Thankyou so much

I finally understood this technique, you made it very clear. thank you so much !!!

*slowly claps* Well presented. Thank you, Sir. You are a legend.

Finally I understand actually what is this. Even in my university I didn't understand this

Sirjee u are genius the way u teach excellent after watching ur videos all doubts are crystal clear God bless u brother

Crystal clear explanation. Tysm!!

Wow !.. finally got it , amazing skills to aproach the topic 👏👏

Your videos now helping in my csir net jrf preparation Thank you ☺☺

Best video on KZfaq that cleared all concepts waoooo... Urdu version b bnaya kren plz apni videos k 😊

Thank you so much ! Your explanation on this topic is phenomenal.

Excellent.. A thorough efforts in making us understand the core concept.. Thanku sir

Good job...perfect explanation...keep up the good work!! 👍

Simpel clear and video wasn't too long, thank you!

best explanation 😎👍👍and helpful for clearing the concept thoroughly 🤠🙏🙏

Thanks sajid Bhai.....It really helped me during exam....

You're amazing I'm not speak your language but I understand your explanation because you are excellent ✔

you're a Lifesaver!

Why the labeled antigen is removed ? Is it have low affinity as compared to unknown antigen??

Got it sir... plz explain about microparticle enzyme immune assay

Excellent explanation n clear understanding pictures tq so much sir

i have watched many videos on youtube but no one can teach like you. thank u sir for well detailed explanation.

Thank you very much for your explanation 🙂🤝

Wooooow super and clearly explanation 😎👍👌👏👏

This was very helpful and easy to understand. Thank you.

Very nice explanation sir...but what is the washing agent we have to use in this process?

wow i just loved it.

Love you this explains

You are the better than everyone else 😍😍😍😍😍 shomu is also not that clear but hats off to u man

Excellent..precise and short explanation

Sir it was just amazing Please keep on making such amazing vedios

You Sir are amazing.... Very wonderful explanation...

Perfect explanation! Thank you!

The part From 7:40 is where the problem has been solved and it's all clear now eventhough the accent is a little hard to understand .. thank u sir!!

Incredible explanation, thank u 🙏🏻 😊

Very easy plus clearly explained.. thankyou so much sir

That's a great help, thank you.

may GOD bless u...pls make more videos

THANK YOU!! Well explained!!

Sir u r simple and awesome teacher.....thank u for helping us.....🙏🙏🙏

Amazing explanation

Thanks a lot. Really well explained

thank you! very well explained

Great explanation. One question though, Why is unlabeled antigen able to displace labeled Ag bound to Ab.... As in why is the unlabeled antigen winning the competition?

Very clear concept. 😊

Very informative and your way of teaching is excellent 👍

Better than shomu's biology video.

Amazing Thank you so much

You r a life saver

Great explanation!

That was excellent! Thank you.

Thanx sir ... u explained it in very easy way

Thank you. It really helps.

Thank yu so much for this awesome video

Very well explained, thank you.

thank you Sajid- great explanation :)

You’re amazing ❤️

Great explanation it helped me a lot.

Thanks sajith

Simply explained.

Thank you so Much!!!

I'm assuming you know the concentration of the radio labeled antigens? Just saying if you take a blood serum sample how will you determine comparison antigen level without knowing the tagged antigens

Awesome explanation!

@Sajid Shaikh is there a way to determine unlabeled antigen or ligand concentration if there were only two solutions? In other words, the first scenario is only adding a known concentration of labeled antigen to some concentration of antibodies. The radioactivity is measured of bound antibody-antigen molecules after removing free antigen. Then, the experiment is repeated with an unknown amount of unlabeled ligand that is added after adding the same known amount of labeled antigen. Ki = kd = 7.4*10^-6 M and concentration of known labeled antigen = 7.4*10^-6 M. What is concentration of unlabeled antigen added in the repeat experiment? Also, radioactivity of bound antibody in the first experiment = 63000 cpm and radioactivity of bound antibody after removing free labeled and unlabeled antigen = 49000 cpm (counts per minute). I am not sure what Ki in this instance means, i.e. Ki = [Ag][Ab]/[AgAb] ? and kd = [Ag*][Ab]/[Ag*Ab]? (where Ag* = radiolabeled antigen) in which case [Ab] would cancel leaving [Ag]/[AbAg] = [Ag*]/[Ag*Ab]. However, I am not sure that is correct. At any rate, I am not sure how to use the information given in this problem. Please anyone let me know if you "see the light" on how to use this information to find concentration of unlabeled antigen [Ag]. Thanks.

Very good explanation thank you❤

Thank you very very much

Thank you! Well explained

Very clear thank you!!

Very nicely explained.. thank u so much🙂

Thanks bro you made me understand it so easy...jazakallah

awessssommmme explanation and verrrry nic sir God bless u

Fantastic! Thank you!

sir , how this antibody is coated to the microtiter plate..?? and how does isotops are tag to particular antigen ??... plz ans i couldn't find from ne other book also

radioimmunoassay can be employed for the detection of insulin in blood plasma for this 125I labelled insulin is mixed and allowed to bind with a known concentration of anti insulin antibody. A known volume of patient blood plasma is then added to the conjugate and allowed to compete with the antigen binding site of antibody. the bound antigen is then separated from the unbound ones and radioactivity of free antigen is then measured by Gama counter . following are some the statement made about this essay . 1 the ratio of radioactive count for the unbound antigen to the bound one is more at the end of reaction. 2.the ratio of radioactive count for unbound antigen to the bound one is less at the end of the reaction. 3 the diabetic patient, the radioactive count for free antigen is less than that for a normal individual 4 A diabetic patient the radioactive count for the free antigen is more than that for the normal individual which of which of the above statement are true?....... Plz sir ans this question in some short explanation plzzzzzzzz

Nice. Thank you!

THANK YOU!

Nice and amazing explanation... Thank you sir...

Thank you this helped alot ... keep up the good work :)

Just awesome 💯

Thanks!! Good work👍

Thanks a bunch

Could you clarify a doubt, why in this technique separates the free antigens (labeled and not) from those complexed to the antibody (labeled and not)?

How to check the radioactivity?? How you measuring it as 40%?

Excellent explanation sir 🤩

superb no wordzzzz

this is great! thx!

Nyc explanation sir

ASSALAMUALAIKUM দাদা , খুব ভালো বোঝালেন , খুব ভালো লাগল

Thanku..u made it very simple...1 question how to detect radio activity?

Thank you so much

thank you sajid . it is really amazing video .pls do more videos