Sajid, is seriously, your explanation and visual methods was SO AMAZING, thanks a lot!!!!
@dr.janpreetsingh51035 жыл бұрын
I have seen many videos on this topic but no one explained like this! this is such a nice explanation!!😊
@Wilina134 жыл бұрын
I missed a question about it in Uworld for USMLE step 1, and their explanation could not help me understand the concept, and you did. Thank you so very much!
@annag274 жыл бұрын
Omg YOU EXPLAINED THIS SO WELL. I get it now.
@IllusionsJunkie6 жыл бұрын
Thank you, this helped a lot! Excellent work! I'm studying in german, but I could still understand everything very well (:
@sajidmicrobiology6 жыл бұрын
Most wecome brother
@hugodesrosiers-plaisance31566 жыл бұрын
Very clear explanation! Thank you!
@sajidmicrobiology6 жыл бұрын
Most welcome
@Immss5 жыл бұрын
I'm very happy now, all my doubts on RIA are cleared, thank u so much sir..😊
@sagorika55525 жыл бұрын
I couldn't understand clearly in my class and was very tensed about it cause my exam is on thrusday.But your explanation gave me relief.very effective video......
@chandlerbing5437 Жыл бұрын
Thank you so much ,saved at least an 2to 3 hours of time and energy....
@Mzee2t2 жыл бұрын
Thanks a lot. This has helped a lot with my physiology course
@savitact79606 жыл бұрын
Way to represent is excellent, covered all the major points in short video, Thankyou so much
@sajidmicrobiology6 жыл бұрын
most welcome
@irissha96754 жыл бұрын
I finally understood this technique, you made it very clear. thank you so much !!!
@kliusd5 жыл бұрын
*slowly claps* Well presented. Thank you, Sir. You are a legend.
@Arif051504 жыл бұрын
Finally I understand actually what is this. Even in my university I didn't understand this
@prabhudasnair56363 жыл бұрын
Sirjee u are genius the way u teach excellent after watching ur videos all doubts are crystal clear God bless u brother
@arvindhkumar8194 жыл бұрын
Crystal clear explanation. Tysm!!
@ArvindSingh-jt6sg3 жыл бұрын
Wow !.. finally got it , amazing skills to aproach the topic 👏👏
@ritujaiswal2863 жыл бұрын
Your videos now helping in my csir net jrf preparation Thank you ☺☺
@sajidmicrobiology3 жыл бұрын
Most welcome
@uroojkhan6555 Жыл бұрын
Best video on KZfaq that cleared all concepts waoooo... Urdu version b bnaya kren plz apni videos k 😊
@zayler_ Жыл бұрын
Thank you so much ! Your explanation on this topic is phenomenal.
@pritpalsinghahluwalia7154 жыл бұрын
Excellent.. A thorough efforts in making us understand the core concept.. Thanku sir
@sajidmicrobiology4 жыл бұрын
Most welcome Pritpal .. would you like to help us by donating small amount to our channel? This will motivate us to make more free videos.
@avanraws775 жыл бұрын
Good job...perfect explanation...keep up the good work!! 👍
@MikeBassil4 жыл бұрын
Simpel clear and video wasn't too long, thank you!
@theanatomy774 Жыл бұрын
best explanation 😎👍👍and helpful for clearing the concept thoroughly 🤠🙏🙏
@AKshay_476 жыл бұрын
Thanks sajid Bhai.....It really helped me during exam....
@sajidmicrobiology6 жыл бұрын
Most welcome akshay
@pharmacist86224 жыл бұрын
You're amazing I'm not speak your language but I understand your explanation because you are excellent ✔
@symoi15692 жыл бұрын
you're a Lifesaver!
@ViralClips9815 жыл бұрын
Why the labeled antigen is removed ? Is it have low affinity as compared to unknown antigen??
@zikrarahman85253 жыл бұрын
Got it sir... plz explain about microparticle enzyme immune assay
@rajukambar19025 жыл бұрын
Excellent explanation n clear understanding pictures tq so much sir
@mohd_athar1232 жыл бұрын
i have watched many videos on youtube but no one can teach like you. thank u sir for well detailed explanation.
@sivakumars78423 жыл бұрын
Thank you very much for your explanation 🙂🤝
@rajikumari88074 жыл бұрын
Wooooow super and clearly explanation 😎👍👌👏👏
@ivyanyango81705 жыл бұрын
This was very helpful and easy to understand. Thank you.
@bhavanishetty24184 жыл бұрын
Very nice explanation sir...but what is the washing agent we have to use in this process?
@PreetiGupta-yg9bt4 жыл бұрын
wow i just loved it.
@chakradharwadhekar61124 жыл бұрын
Love you this explains
@prateekpanda42244 жыл бұрын
You are the better than everyone else 😍😍😍😍😍 shomu is also not that clear but hats off to u man
Sir it was just amazing Please keep on making such amazing vedios
@preciousezeobi57704 жыл бұрын
You Sir are amazing.... Very wonderful explanation...
@menasalman85344 жыл бұрын
Perfect explanation! Thank you!
@sajidmicrobiology4 жыл бұрын
Most welcome mena.. would you like to help us by donating small amount to our channel? This will motivate us to make more free videos.
@menasalman85344 жыл бұрын
@@sajidmicrobiology Yes!
@sajidmicrobiology4 жыл бұрын
Thanx mena.. you can paytm us on 8830061003. Your small help can make big diffrence.
@maryem28804 жыл бұрын
The part From 7:40 is where the problem has been solved and it's all clear now eventhough the accent is a little hard to understand .. thank u sir!!
@sajidmicrobiology4 жыл бұрын
Most welcome.. it hard to talk english for me
@razanathamneh79064 жыл бұрын
Incredible explanation, thank u 🙏🏻 😊
@sajidmicrobiology4 жыл бұрын
Most welcome razan .. would you like to help us by donating small amount to our channel? This will motivate us to make more free videos.
@poeticvilla81393 жыл бұрын
Very easy plus clearly explained.. thankyou so much sir
@ogroogrom39334 жыл бұрын
That's a great help, thank you.
@sajidmicrobiology4 жыл бұрын
Most welcome
@ronaldjamatia71196 жыл бұрын
may GOD bless u...pls make more videos
@sorlos11 ай бұрын
THANK YOU!! Well explained!!
@chaitrachethanmeena31115 жыл бұрын
Sir u r simple and awesome teacher.....thank u for helping us.....🙏🙏🙏
@sajidmicrobiology5 жыл бұрын
Most welcome dayana
@sidrasarwar67213 жыл бұрын
Amazing explanation
@agarwalshivam8446 жыл бұрын
Thanks a lot. Really well explained
@sajidmicrobiology6 жыл бұрын
Most welcome
@armella.h5 жыл бұрын
thank you! very well explained
@mahimakapoor99825 жыл бұрын
Great explanation. One question though, Why is unlabeled antigen able to displace labeled Ag bound to Ab.... As in why is the unlabeled antigen winning the competition?
@sajidmicrobiology5 жыл бұрын
Yes... it's due to competition... like substrate of enzyme and inhibitor
@mahimakapoor99825 жыл бұрын
@@sajidmicrobiology Ohh so it's basically due to high concentration of unlabelled Ag vs labelled one... Thanks
@sayanighosh78725 жыл бұрын
Very clear concept. 😊
@soniamirza48334 жыл бұрын
Very informative and your way of teaching is excellent 👍
@sajidmicrobiology4 жыл бұрын
Thanx.. whr r u from ?
@soniamirza48334 жыл бұрын
@@sajidmicrobiology lahore
@sajidmicrobiology4 жыл бұрын
Ok
@soniamirza48334 жыл бұрын
@@sajidmicrobiology sir how can we measure the radioactivity?
@sajidmicrobiology4 жыл бұрын
With the help of scintillation counter
@Shashwat_Lakhera5 жыл бұрын
Better than shomu's biology video.
@mitalogy71089 ай бұрын
U r right 👍
@user-wi3nu3cp7z4 жыл бұрын
Amazing Thank you so much
@curryfromscratch52863 жыл бұрын
You r a life saver
@sajidmicrobiology3 жыл бұрын
Contact for Personal or group immunology crash course (online) saj.99999@gmail.com
@reemariaz93972 жыл бұрын
Great explanation!
@emanuelgrant43356 жыл бұрын
That was excellent! Thank you.
@shivanisah18506 жыл бұрын
Thanx sir ... u explained it in very easy way
@sajidmicrobiology6 жыл бұрын
Most welcome Shivani Duggal
@swatisangwan49256 жыл бұрын
Thank you. It really helps.
@sanusandhyaraina29395 жыл бұрын
Thank yu so much for this awesome video
@sheetal2023Bio5 жыл бұрын
Very well explained, thank you.
@odedile85 жыл бұрын
thank you Sajid- great explanation :)
@thecerebrum.5 жыл бұрын
You’re amazing ❤️
@dorian_arno Жыл бұрын
Great explanation it helped me a lot.
@imsaiarasan84154 жыл бұрын
Thanks sajith
@kaesavr.p14734 ай бұрын
Simply explained.
@silviaazara80862 жыл бұрын
Thank you so Much!!!
@jayblack86913 жыл бұрын
I'm assuming you know the concentration of the radio labeled antigens? Just saying if you take a blood serum sample how will you determine comparison antigen level without knowing the tagged antigens
@Jaysay75 жыл бұрын
Awesome explanation!
@sajidmicrobiology5 жыл бұрын
Thanx... where r u from?
@jakedolezal8715 жыл бұрын
@Sajid Shaikh is there a way to determine unlabeled antigen or ligand concentration if there were only two solutions? In other words, the first scenario is only adding a known concentration of labeled antigen to some concentration of antibodies. The radioactivity is measured of bound antibody-antigen molecules after removing free antigen. Then, the experiment is repeated with an unknown amount of unlabeled ligand that is added after adding the same known amount of labeled antigen. Ki = kd = 7.4*10^-6 M and concentration of known labeled antigen = 7.4*10^-6 M. What is concentration of unlabeled antigen added in the repeat experiment? Also, radioactivity of bound antibody in the first experiment = 63000 cpm and radioactivity of bound antibody after removing free labeled and unlabeled antigen = 49000 cpm (counts per minute). I am not sure what Ki in this instance means, i.e. Ki = [Ag][Ab]/[AgAb] ? and kd = [Ag*][Ab]/[Ag*Ab]? (where Ag* = radiolabeled antigen) in which case [Ab] would cancel leaving [Ag]/[AbAg] = [Ag*]/[Ag*Ab]. However, I am not sure that is correct. At any rate, I am not sure how to use the information given in this problem. Please anyone let me know if you "see the light" on how to use this information to find concentration of unlabeled antigen [Ag]. Thanks.
@shubhrasmitatola69513 жыл бұрын
Very good explanation thank you❤
@zehra3666 жыл бұрын
Thank you very very much
@nyawirawaithaka49934 жыл бұрын
Thank you! Well explained
@sajidmicrobiology4 жыл бұрын
Most welcome
@alessandroinnocenti2352 жыл бұрын
Very clear thank you!!
@namitanepal7984 жыл бұрын
Very nicely explained.. thank u so much🙂
@laraibkanwal69973 жыл бұрын
Thanks bro you made me understand it so easy...jazakallah
@aliraza-hc4nw5 жыл бұрын
awessssommmme explanation and verrrry nic sir God bless u
@felopeeontube6 жыл бұрын
Fantastic! Thank you!
@yamaatomayi43156 жыл бұрын
sir , how this antibody is coated to the microtiter plate..?? and how does isotops are tag to particular antigen ??... plz ans i couldn't find from ne other book also
@sajidmicrobiology6 жыл бұрын
For coating , coating buffers like bicarbonate of pH 9 are used... for making particular molecular radiolabelled... radioactive nutrients are supplied to the producer cells
@kavitachawla33065 жыл бұрын
radioimmunoassay can be employed for the detection of insulin in blood plasma for this 125I labelled insulin is mixed and allowed to bind with a known concentration of anti insulin antibody. A known volume of patient blood plasma is then added to the conjugate and allowed to compete with the antigen binding site of antibody. the bound antigen is then separated from the unbound ones and radioactivity of free antigen is then measured by Gama counter . following are some the statement made about this essay . 1 the ratio of radioactive count for the unbound antigen to the bound one is more at the end of reaction. 2.the ratio of radioactive count for unbound antigen to the bound one is less at the end of the reaction. 3 the diabetic patient, the radioactive count for free antigen is less than that for a normal individual 4 A diabetic patient the radioactive count for the free antigen is more than that for the normal individual which of which of the above statement are true?....... Plz sir ans this question in some short explanation plzzzzzzzz
@sajidmicrobiology5 жыл бұрын
Option 1 and 4 seem to be correct ...
@kavitachawla33065 жыл бұрын
Thank u sir
@fredmd1015 жыл бұрын
Nice. Thank you!
@nastia71973 жыл бұрын
THANK YOU!
@sajidmicrobiology3 жыл бұрын
Whr r u from?
@nastia71973 жыл бұрын
@@sajidmicrobiology ukraine, your video really helped me to prepare for a presentation about RIA( immunology at the university)
@sajidmicrobiology3 жыл бұрын
Glad to know
@deepakvs23425 жыл бұрын
Nice and amazing explanation... Thank you sir...
@smash20096 жыл бұрын
Thank you this helped alot ... keep up the good work :)
@nagalakshmichallapalli7737 Жыл бұрын
Just awesome 💯
@achithswarnachandra3655 жыл бұрын
Thanks!! Good work👍
@muhammed-kasimonimisisan-ci1co11 ай бұрын
Thanks a bunch
@ciroesposito53952 жыл бұрын
Could you clarify a doubt, why in this technique separates the free antigens (labeled and not) from those complexed to the antibody (labeled and not)?
@mitraarjunan49 Жыл бұрын
How to check the radioactivity?? How you measuring it as 40%?
@user-qt1pg6pb5x6 ай бұрын
Excellent explanation sir 🤩
@anishamn21724 жыл бұрын
superb no wordzzzz
@sajidmicrobiology4 жыл бұрын
Thanx anisha... whr r u from ?
@melodychao85295 жыл бұрын
this is great! thx!
@ImranAhmad-df3mi5 жыл бұрын
Nyc explanation sir
@pritambeg11244 жыл бұрын
ASSALAMUALAIKUM দাদা , খুব ভালো বোঝালেন , খুব ভালো লাগল
@somosreeghosh86626 жыл бұрын
Thanku..u made it very simple...1 question how to detect radio activity?
@sajidmicrobiology6 жыл бұрын
Thanx Somosree
@sajidmicrobiology6 жыл бұрын
Radioactivity is tested by scintillator counter
@somosreeghosh86626 жыл бұрын
Sajid Shaikh thanku again :)
@rameshpadha19744 жыл бұрын
Thank you so much
@sakthidharshni59756 жыл бұрын
thank you sajid . it is really amazing video .pls do more videos