Sanger sequencing method - dideoxy sequencing of DNA

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Sanger sequencing method lecture - This lecture explains about the dideoxy chain termination method of DNA sequencing which is also known as Sanger sequencing. The mechanism of Sanger sequencing is explained with this video lecture. In this DNA sequencing approach, modified dideoxy nucleotides are used to cause the chain termination of DNA synthesis which helps to find out the exact DNA sequence. This method of DNA sequencing is named as following -
Sanger sequencing
Dideoxy sequencing
Chain termination method of DNA sequencing.
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Thank you for watching the DNA sequencing lecture on dideoxy chain termination method also known as the Sanger sequencing of DNA.

Пікірлер: 899

@shahanazshubhan7648 5 жыл бұрын

You're the best teacher. You make everything so enjoyable and easy to understand which i like the most. Couldn't thank you enough. 😇

@shomusbiologyofficial 5 жыл бұрын

Thank you so much for appreciating my efforts

@sarangas8688 4 жыл бұрын

Excellent ! Good explanation But voice is little unclear

@v.saikiranv.saikiran9752 4 жыл бұрын

Sir please explanation chemicals to indentiy activesite arg, his, lys, his

@zarlashtkhan3540 3 жыл бұрын

@@shomusbiologyofficial in case of ddgtp how can be a 3 nucleotide sequence formed if both the consective gtps ar ddgtp please answer

@misha9672 3 жыл бұрын

@@zarlashtkhan3540 it is just because incorporation of ddgtp is a random process. It is not necessary that every complementarity of A is DDG. G can also be complementared. ddntps are provided in low concentration in the mixture than regular nucleotides( A, T,G,C). It means regular nucleotides are also always present. So incorporation of ddgtp is a random probability( sometimes in the beginning complementary to 2nd C, sometimes to the 3rd C, sometimes to the last C). Hope it helps.

@gerganabonev 4 жыл бұрын

There are all 4 dNTPs added in each reaction and also one of each ddNTP in each well is added because we want to produce fragment with different length. Otherwise the reaction would have stop at the first complementary ddNTP added after the primer. and there will be only 4 length fragments.

@aboulfazlrad6482 4 жыл бұрын

Exactly and thanks for mention this, otherwise it does not make sense. So I suggest to edit this video.

@red8527 4 жыл бұрын

Yeah, thanks for clearing that. I was wondering how he got 2 GTP & 3 GTP as well. It should have terminated at 2 GTP position only if 3 dNTPs & one ddNTP were used.

@sowhatsnew6271 3 жыл бұрын

Thanks .now i am understand

@thoibathongam5012 3 жыл бұрын

Exactly... Thanx for supplementary this point.

@manashpratimsarmah4487 3 жыл бұрын

Absolutely right 😊.. I want to write the same thing

@dannydaily20 Жыл бұрын

Arguably one of the best teachers I've come across on the internet. Man with you every tough thing feels too easy.. Grateful 🌸

@shomusbiologyofficial Жыл бұрын

Thank you so much for appreciating my efforts

@emilyparks8635 3 жыл бұрын

This is by far the most helpful explanation of Sanger Sequencing; it's a whole walk-through of how/why the process works (rather than just pieces/bits of information from other videos). Thank you very much!

@shomusbiologyofficial 3 жыл бұрын

You're welcome. Glad to hear that you're getting benefit from my lectures

@madhavi7295 4 жыл бұрын

I am a second yr medical student and i really can't express how helpful this and your other videos on genetics have been to me ! You are a wonderful teacher ! Thanks a lot for teaching the topic so beautifully.

@shomusbiologyofficial 4 жыл бұрын

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@msnadeem837 3 жыл бұрын

Nice presentation. However, as a colleague I would suggest you to add 4 dNTPs plus limiting amount of ddNTP in each reaction tube. Otherwise, you will only get the chain terminated at the first nucleotide such as G, and no next G can be detected....

@isha8349 Жыл бұрын

EXACTLY .I WAS CONFUSED THERE!!

@eugenekim3037 6 жыл бұрын

Shomu knows how to explain DNA sequencing very very effectively. I could not understand the ins and outs of the Sanger DNA sequencing until he went into explaining the method step by step, beginning with the explanation of dideoxy chain termination. I plan to watch all of his videos on DNA sequencing.

@ilaila3504 3 жыл бұрын

shomu is the best thing that has ever happened to my education. Thankyou Shomu

@shomusbiologyofficial 3 жыл бұрын

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@khinphyusin9932 2 жыл бұрын

The best teacher ever and every complicated lecture becomes easy and crystal clear after watching ur videos. I thank u from the bottom of my heart prof.

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@Ghanabless 4 жыл бұрын

Doing a masters in cancer and I can't tell you how much you have helped simplify topics.

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@mattyfestating 2 жыл бұрын

I am here again because my professor has sent your link but I have already subscribed to your channel since high school times. You make me understand better. Thank you so much for all these years.

@shomusbiologyofficial 2 жыл бұрын

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@deeprobanerjee7622 4 жыл бұрын

Excellent explanation.. before watching this lecture, I tried to read a review paper on sequencing published in the Genomics journal, watched a lecture by Worcester Polytechnic Institute but you, my friend, are the real deal.. Thanks!!

@shomusbiologyofficial 4 жыл бұрын

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@harshanawimalarathna9039 2 жыл бұрын

Hellow sir I am from Sri Lanka.You are the best biology teacher that I have ever seen.I and my almost all colleagues in our university are following your channer and have achieved highest grades because of your fantastic teaching ability.I highly appriciate it.GOOD LUCK SHOMU'S BIOLOGY!!!!!!!

@tamizhgirl 5 жыл бұрын

Hi sir...Without you and your channel i couldn't get pass in the subject..For each and every semester I'm learning from u..ur trying hard to make us understand so easily..Thanks a lot for your effort...I became your fan since 2017....If u reply i ll be happy...Thank u sir...

@shomusbiologyofficial 5 жыл бұрын

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@snehal1689 5 жыл бұрын

Today is my exam(3rd. yr. B.Tech) & I have completed my whole syllabus a night before in just 3.5 hrs. by watching your videos on various topics !!😁 Thank you sir .. U are the best 👍 simplicity 💯🌟

@shomusbiologyofficial 5 жыл бұрын

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@mollyb3185 5 жыл бұрын

@@shomusbiologyofficial in first test tube why don't we add all the dntps n only three please clear my doubt , appearing for my final MD exams

@kidusbahiru4369 5 жыл бұрын

Wow, I wish my professor taught this way. Now I understand what I read in the textbook and slides. Thank you

@shomusbiologyofficial 5 жыл бұрын

Glad to hear that you are getting benefit from the videos

@user-rr3ny6cg1n 3 жыл бұрын

I agree that you are the best teacher I've ever seen in online. I can keep enjoy of studying biology thanks to you. You help me a lot! Thank you ,sir. From Korea

@aadarniya 5 жыл бұрын

4th sem biotech student here. You're awesome. College mein shaam ke 6 baje padhaya thha Sanger Sequencing, kuch nahi samjha. Now I'm quite clear about Sanger Sequencing. Thanks a lot.

@shomusbiologyofficial 5 жыл бұрын

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@shashpalsidhu2420 4 ай бұрын

Thankyou so much , your videos have been helping me since my bachelors and now my masters as well

@shomusbiologyofficial 4 ай бұрын

You're welcome. Glad to hear that you're getting benefit from my lectures

@shafisirbiology760 5 жыл бұрын

@7:00 here not only 3dntps in each reaction centre but all 4dntps and one of each ddntp in each well is added

@awaisahmadsadat2313 4 жыл бұрын

point to be noted.

@brunothebrowndog 5 жыл бұрын

You are the best Biology teacher in the world

@shomusbiologyofficial 5 жыл бұрын

Thank you for appreciating my efforts

@colemimiralte7025 5 жыл бұрын

yes he is😊

@sofiastar9197 4 жыл бұрын

Thank you so much ...this is the first time I got the Sanger sequencing ..you put the whole process together in one lecture now its easy to remember the whole story...before I used to learn parts of Sanger sequencing and could not connect different parts..👍

@shomusbiologyofficial 4 жыл бұрын

You're welcome. Glad to hear that you're getting benefit from my lectures

@khalidabdulshafi2835 5 жыл бұрын

You explained a difficult concept in a beautiful, simple way. Thank you!

@shomusbiologyofficial 5 жыл бұрын

Glad to hear that you are getting benefit from the videos

@oishisaha5932 4 жыл бұрын

It's really help me to understand the actual process of Sanger sequencing...so clearly & easily...

@shomusbiologyofficial 4 жыл бұрын

You're welcome. Glad to hear that you're getting benefit from my lectures

@ZoyaKhan-pp2kr 5 жыл бұрын

Your videos helped me a lot during my PhD journey. May God bless you. Thank you

@kapushkapolis 2 жыл бұрын

I couldn't understand it when my professor was explaining it. But you've just explain AMAZING!!! You are the best!!!!

@shomusbiologyofficial 2 жыл бұрын

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@NEWLAROEKOE 6 жыл бұрын

You sire, are the reason Im more confident about my job interview today! I ll donate money if I get this job!!

@fatimashaheen1474 3 жыл бұрын

You are blessing, i wish i get teacher like you in university, u clear every single confusing without even asking!

@shomusbiologyofficial 3 жыл бұрын

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@bahahos7426 3 жыл бұрын

Thank you very much, i study biomedical lab.teknologi, and i startet in 2017, and your videos have been very helpful for me, i appreciate it ,and thank you very mig wee love you

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This guy is THE BEST!!! Thank you soooo much.

@shomusbiologyofficial 2 жыл бұрын

You're welcome

@asmaparadis8509 5 жыл бұрын

YOU ARE AMAZING!! thank you so much mister Suman. Plz keep up what you do. You help so many students from all over the world by explaining these bio topics so easily. I have binge-watched a lot of your video's and passed my exam. Thank you thank you thank you.

@shomusbiologyofficial 5 жыл бұрын

Glad you liked my lectures. please subscribe to our channel

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You can't come across this guy and fail to subscribe 👏🏽👏🏽👏🏽🙌🏼

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@shomusbiologyofficial 2 жыл бұрын

Thank you so much for appreciating my efforts

@macktalreja6946 7 жыл бұрын

Amazing to say that ... the very 1st channel i search to clear my thoughts is Shomu's Biology from a year...and its really helpfull...thank you..

@shomusbiologyofficial 7 жыл бұрын

+Mack Talreja you're welcome. Glad to hear that.

@H.M1212 4 жыл бұрын

This is the best lecture. I understand every step very well when you explain . Thank you very much for making it easy and understandable!

@shomusbiologyofficial 4 жыл бұрын

Glad to hear that you're getting benefit from my lectures

@sudhanshukumar-lw3hq 3 жыл бұрын

You are doing very good work keep doing this work it helps a lot people

@shomusbiologyofficial 3 жыл бұрын

Thank you so much for appreciating my efforts

@gvaradan 2 жыл бұрын

Awesome. Tough concept explained very simple. Got confused a bit with why we got 2 and 3 GTP. But after reading posts below I understood the concept.

@shomusbiologyofficial 2 жыл бұрын

Thank you

@varshagoyal1081 Жыл бұрын

Please explain

@muhammadabdullah2735 2 жыл бұрын

exactly what my teacher trying to teach us. Now it is clear to me.

@shomusbiologyofficial 2 жыл бұрын

Thank you so much for appreciating my efforts

@user-dp3vr7ip8w 9 ай бұрын

This was great sir! Very well explained. Thank you so much for this precise explanation.

@shomusbiologyofficial 9 ай бұрын

Glad to hear that you're getting benefit from my lectures

@meganevans8006 3 жыл бұрын

I can explain the Sanger method now. Thank you!

@shomusbiologyofficial 3 жыл бұрын

Glad to hear that

@VishvajeetSingh-hc6og 4 жыл бұрын

you are the best teacher of bio . Thank you sir for best video.

@shomusbiologyofficial 4 жыл бұрын

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@ashganahmed2587 2 жыл бұрын

Great ..You make very complicated things so simple...

@shomusbiologyofficial 2 жыл бұрын

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@mahdisamaninezhad7718 4 жыл бұрын

this video was so wonderful and these videos open new windows about biology.

@shahbaloch4814 3 жыл бұрын

Very helpful and best method of teaching Sir ap bohoth he Acha samjathay he hor padathay he

@shomusbiologyofficial 3 жыл бұрын

Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures

@dr.abhishekyadav696 7 жыл бұрын

in all the tubes all dNTP's are added, otherwise how would the chains be of different length. For eg in 1st tube according to your method, the chain should terminate at the 1st A attachment only. Thus all chains would be of same length

@esmaboukerzaza6266 7 жыл бұрын

same remark !

@CrazyGamer-xi8rf 6 жыл бұрын

Exactly

@biswanath5941 6 жыл бұрын

Each tube needs one ddnucleotide sequence for termination. Different sequence undergoes different length if they have fluctuating sequence. This is only applied for short sequences, and worth of a Nobel price😊

@faheemdawar6696 6 жыл бұрын

Each tube have all narmal dntps but one specific ddntps.

@AkshitVats 6 жыл бұрын

abhishek yadav the quantity of di deoxyNTP are lesser as compare to the dNTP. Hence there are chances of getting dd on the right place as well as d. During electrophoresis it can give larger bands if the dNTPs are added everytime but ddNTP can also bind

@smithasaleesh14 3 жыл бұрын

very useful.most of the lectures well describes the steps untill autoradiography,for an unexpert person from that step hurdles starts.this videos thoroughly clarified such a gassy knowledge.thank you so much

@shomusbiologyofficial 3 жыл бұрын

You're welcome

@dorkjet 11 ай бұрын

I just couldn't thank you enough. Thank you Sir.

@shomusbiologyofficial 11 ай бұрын

You're welcome

@benalipozenezarinllen1668 5 жыл бұрын

Thank you Sir..You simplify and explain the concepts very nicely!!

@shomusbiologyofficial 5 жыл бұрын

Glad you liked my lectures

@md.giashuddin6057 5 жыл бұрын

When the chain get terminated at 2nd position ( C is present)then why will it again proceed to terminate at 3rd position ( again C is present)?

@amishbutle4887 4 жыл бұрын

Hey bro ur question is valid. Shomu has taught in a little wrong way in 1st test tube there should be both ddATP and dATP thn by probability either of them would attach

@ShivaKumar-sb4zi 4 жыл бұрын

@@amishbutle4887 yes bro 👍

@ShivaKumar-sb4zi 4 жыл бұрын

Same question I wanted to type but after seeing your answer I understood 👍😊

@anikdasad9 4 жыл бұрын

@@amishbutle4887 thanks bro..I also have same question..

@arjunp9079 4 жыл бұрын

👍

@bijitjoy994 3 жыл бұрын

Thank you shomus biology..you are the best teacher i have ever seen

@muhammedyousafk4115 2 жыл бұрын

Thankyou sir, Very effective class💙 May Allah bless you with more and more knowledge

@shomusbiologyofficial 2 жыл бұрын

Thank you so much for appreciating my efforts

@chrmcstr4166 3 жыл бұрын

You don't know how much you helped me ...thanks a lot ❤️

@shomusbiologyofficial 3 жыл бұрын

You're welcome. Glad to hear that you're getting benefit from my lectures

@alibupatrickdennis5066 Жыл бұрын

Precise and straight to the point, well understood

@shomusbiologyofficial Жыл бұрын

Thank you

@meherrrr1998 4 жыл бұрын

i'm Meherima from bangladesh , a student of Genetic engineering....just love your way of all videos ,these are really so helpful Shomu, God bless you....thank u so much for all these contributions....

@subhampradhan665 4 жыл бұрын

Sir you are the best. I have no. Words to explain. You make it understand so simple and explains the best.❤️😇

@shomusbiologyofficial 4 жыл бұрын

You're welcome. Glad to hear that you're getting benefit from my lectures

@CestlaBios 7 жыл бұрын

It's a great explanation, but as other comments have mentioned, you are a little ambiguous when explaining how the sequence can continue once the ddNucleotide is added. It is necessary to emphazise that each of the four mixtures contains ALL FOUR dNucleotide PLUS one of the four ddNucleotide. Otherwise, thanks for the clear explanation.

@MrChips004 5 жыл бұрын

You Clarified further, Kudos!

@kashafseducationalsite363 4 жыл бұрын

thaks .. u cleared my confusion .. about d/ff sizes of sequence.....

@onionyang7999 4 жыл бұрын

thanks！

@678uio 3 жыл бұрын

I enjoy your way of talking and helps me to get ur every point. From pakistan.

@shomusbiologyofficial 3 жыл бұрын

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@malshihirunika5591 Жыл бұрын

Love the way you teach.... can understand easily.... Thankyou so much ❤

@shomusbiologyofficial Жыл бұрын

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@anamijaz4676 5 жыл бұрын

amazing! I have biology paper tomorrow. Your lecture is so helpful

@shomusbiologyofficial 5 жыл бұрын

Thank you. Glad you liked my lectures

@anweshaghosh3748 7 ай бұрын

Thank you sir.... I can't understand this experiment in my class.... I'm struggling to understanding this but not able...but after watching this video I understand this clearly

@shomusbiologyofficial 7 ай бұрын

All the best. Glad to hear that you're getting benefit from my lectures

@trishabhattacharyya1918 4 жыл бұрын

The best video I found on the internet wrt to Sanger Sequencing. Thank you so much!

@reemalamassi3494 3 жыл бұрын

brother you saved me alot ! thank you very much

@shomusbiologyofficial 3 жыл бұрын

You're welcome. Glad to hear that you're getting benefit from my lectures

@subhanjaligupta1250 3 жыл бұрын

Thank you so much sir.. You're simply amazing.. Your way of teaching is so relaxing... And makes every complications so easy within minutes.. Much respect for you sir ❤🙏

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@aadarshguptabest.9406 2 жыл бұрын

And my doubt or concept clearly solve again here like always ❤️ the one of best teaching way till now I know ❤️

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@ertzor 6 жыл бұрын

You are really saving my ass in my clinical imaging course with these awesome lectures man. Thanks for the good and easy to understand explanations! Liked and subscribed.

@shomusbiologyofficial 6 жыл бұрын

+Ertzor thank you. Glad you liked my lectures. Please subscribe and share

@deepanshi199 3 жыл бұрын

thank you sir for explaining this in simple and understandable manner

@shomusbiologyofficial 3 жыл бұрын

You're welcome. Glad to hear that you're getting benefit from my lectures

@youme7815 3 жыл бұрын

Sir you're awesome and a life saver. Loving your lessons from Turkey. Thanks a lot.

@rukaiyamemon9908 9 ай бұрын

Wow u have Just got magic to Simply such complex things thanks for vedio sir

@shomusbiologyofficial 9 ай бұрын

Glad to hear that you're getting benefit from my lectures

@divineflower1466 5 жыл бұрын

Thank you so much Sir... No single "difficult" topic is left after I listen to your explanations... So grateful for that... ❤

@shomusbiologyofficial 5 жыл бұрын

Glad it helped. No topic is beyond explanation

@fromindiatocanadayuti5239 6 жыл бұрын

U r SAVAGE..thanku so much... helpful at last moment.. keep on adding such videos..specially related to biotech..

@Emankhalid_29 4 жыл бұрын

Explained in very easy and grasping terms. Thank youu!

@shomusbiologyofficial 4 жыл бұрын

You're welcome

@fatimamangera7784 5 жыл бұрын

After reading from notes which is given by sir and also from slide share ... but not clear with concept... n have confusion .. here i am at 2 am watching ur video... n nw 100% clear with basic concept of method.... n clr with all my doubts.... thank u soooo much... 💕

@shomusbiologyofficial 5 жыл бұрын

Glad you liked my lectures

@dojaqaraqe1220 5 жыл бұрын

Amazing , helped simplify the concept and really helped me understand!!

@shomusbiologyofficial 5 жыл бұрын

You're welcome

@syedaafsananushrat890 2 жыл бұрын

I don't know-how could be some people dislike the video. U r a lifesaver boss. thanks a lot for the video.

@shomusbiologyofficial 2 жыл бұрын

Thank you so much for appreciating my efforts

@rajeshregmi8505 6 жыл бұрын

I have watched ur many videos bt dis time u really helped me before one day of examination...thanks from nepal

@sabeenmurad6328 5 жыл бұрын

Sir u r a genius . It was Soo difficult for me and u made it Soo easy

@shomusbiologyofficial 5 жыл бұрын

Thank you so much for appreciating my efforts

@venkatvaibhav551 6 жыл бұрын

I'm actually mad at myself that I'm only finding out about this channel, a day before my exam smh Shomu you are actually a genius

@samansiddiqui4257 6 жыл бұрын

Very well taught up till now I was unable to understand sequencing but after watching this video I cleared my concepts thanku shomu

@sangeethau5047 3 жыл бұрын

Very good convenient way of your teaching make understand even difficult concepts.. thank you sir..

@shomusbiologyofficial 3 жыл бұрын

Thank you so much for appreciating my efforts

@afeedarazik4738 5 жыл бұрын

All dNTPs along with 1 particular ddDNTP is added in each tube. As a result of chain termination DNA fragments of different lengths are formed. Otherwise, if we are adding only ddNTP along with 3 dNTP, termination will occur only in the first one.

@moolepriyanka 2 жыл бұрын

How different length fragments are formed by adding same ddNTP in particular reaction mixture the first fragment is terminated due to particular ddNTP attachment,why don't the same ddNTP attached at the same position again, why it's not happening? Will u plss explain to me

@renoleevans Жыл бұрын

OMG! Just easily explained! You just gained another subscriber!

@shomusbiologyofficial Жыл бұрын

Thank you

@aapkamedico6657 4 жыл бұрын

Superb sir...u r Teaching every concept so thoroughly... Love it....

@shomusbiologyofficial 4 жыл бұрын

You're welcome. Glad to hear that you're getting benefit from my lectures

@chetanshiurkar Жыл бұрын

After the 5 years of uploading this video , its very much helpful for me for my seminar 🙂 Thank you sir🙂

@shomusbiologyofficial Жыл бұрын

All the best

@shristidahal9650 2 жыл бұрын

God of biology. You are great

@shomusbiologyofficial 2 жыл бұрын

Thank you so much for appreciating my efforts

@aswiniraghu6098 5 жыл бұрын

Sir, You really really helped me in my exams..Words are not enough to express my gratitude...

@TheNatureandScience 3 жыл бұрын

I learn this easily from you....

@shomusbiologyofficial 3 жыл бұрын

Thank you

@mayurbiotech1657 5 жыл бұрын

I wonder how cud DNA is still synthesized, even after adding ddNTPs in case of C

@amritarathore2119 7 жыл бұрын

Thank u sooo much sir for ur vedios....i m fully dependent on u for my 12th board....no one can teach biotechnology better than u.....

@luljetegecaj8195 5 жыл бұрын

You're amazing!! Such a clear and precise explanation. Bless your soul!

@shomusbiologyofficial 5 жыл бұрын

Thank you. Glad you liked my lectures. Please subscribe and share

@nooraibham5285 4 жыл бұрын

Wow...you are such a great great teacher...! Kudos to you!!! Thank you very much!!!

@shomusbiologyofficial 4 жыл бұрын

Thank you. Glad you liked my lectures

@meenakshikumari7821 5 жыл бұрын

A lot of Thank sir i have no idea for dna sequencing but now i feel confident bcoz of you sir...... When i have any problem i see ur videos sir then my problem is solved by u indirectly .... Tysm again sir U r so intelligent sir

@shomusbiologyofficial 5 жыл бұрын

Glad to hear that you are getting benefit from the videos

@sayakgain6748 2 жыл бұрын

Perfect explanation... I clearly understand it. Thank you so much sir. 🙏

@shomusbiologyofficial 2 жыл бұрын

Thank you so much for appreciating my efforts

@arushetickoo1700 6 жыл бұрын

Instead of using only ddNTP we also use the same nucleotide otherwise a reaction for ddGTP will stop at 2nd step only and it will not move further to 3rd or 7th step without a free OH group.

@eldrienadsilva3385 3 жыл бұрын

Thanks a lot, sir. These videos really help us get through college. 🥺😄

@shomusbiologyofficial 3 жыл бұрын

You're welcome. Glad to hear that you're getting benefit from my lectures

@rajeshmadaan3065 6 жыл бұрын

Thankyou for all the videos. U make understanding things easier. Well done.

@faryal1999 2 жыл бұрын

You deserve award maannn🙌🏻💟

@shomusbiologyofficial 2 жыл бұрын

Thank you so much for appreciating my efforts

@kalyahnn1927 3 жыл бұрын

U made my assignment easy tq sooo much sir

@shomusbiologyofficial 3 жыл бұрын

You're welcome. Glad to hear that you're getting benefit from my lectures

@taraxxx1993 5 жыл бұрын

Thank you so so much, before watching this video I really thought that I’m too dumb to understand this method haha but now I understand it very well

@shomusbiologyofficial 5 жыл бұрын

All the very best

@amjadiqbal783 3 жыл бұрын

You are a wonderful teacher of Biology.. Whenever i feel any topic difficult to teach I come to ur channel...it always helps me out.. God bless you boy..

@shomusbiologyofficial 3 жыл бұрын

Thank you so much for appreciating my efforts

@ommayhany65 5 жыл бұрын

Thank you so much.your all vedios are really great helpful for us...I am thankful you to giving us such a wanderful opportunity so that we can learn easily.. understand easily

@shomusbiologyofficial 5 жыл бұрын

Glad I can help