Southern Blotting | Basic Procedure

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Southern Blotting | Basic Procedure | In Hindi

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Күн бұрын

#Southern #Blotting #BaaYo
Southern blotting is a hybridization technique for identification of particular size of DNA from the mixture of other similar molecules. This technique is based on the principle of separation of DNA fragments by gel electrophoresis and identified by labelled probe hybridization.Basically, the DNA fragments are separated on the basis of size during electrophoresis. Separated DNA fragments after transferring on nylon membrane, the desired DNA is detected using specific DNA probe that is complementary to the desired DNA.A hybridization probe is a short (100-500bp), single stranded DNA. The probes are labeled with a marker so that they can be detected after hybridization.
Procedure/ Steps
1. Restriction digest: by RE enzyme and amplification by PCR
2. Gel electrophoresis
3. Denaturation: Treating with NaOH
4. Blotting
5. Baking and Blocking with casein in BSA
6. Hybridization using labelled probes
7. Visualization by autoradiogram
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@BaaYo Жыл бұрын

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@rishuyadav9667 3 жыл бұрын

I have hardly seen teachers with such conceptual clearity and confidence like you. Warna aajkal to sab bs book se as it is copy paste krte h blindly. Also use of regional language is appreciated. 👍 Commendable.

@pronoobvines6178 4 жыл бұрын

Really 1st time in my life I found a madam with lots of concept and basic knowledge 😁😁😁. Sorry if I hurt anyone

@BaaYo 4 жыл бұрын

Thank you MR.

@Salmankhan-dx1do 7 ай бұрын

🤫

@anitathakur4514 3 ай бұрын

Same here mam nhi hota to pta ni ky hota

@zeeshantayyabi1392 4 жыл бұрын

A big big big thankkkk u ma'am.......u have helped out me with great difficulty in this topic.......hatsoff to ur explaination🙌🙌

@dilipsangam2091 3 жыл бұрын

Wonderful

@dilipsangam2091 3 жыл бұрын

Wonderful Presentation.

@parnikavishe3761 4 жыл бұрын

Hello am I am a student of SY biotechnology and I regularly watch your videos and they prove very helpful to me especially during exam times. Thank you so much for such amazing lectures, keep making these videos they are really helpful 😊

@anamikabhardwaj5170 11 ай бұрын

Seriously last moment pe video dekh Rahi aapka. And it's awesome... thanks alott...ekdum crystal clear concept...😊😊

@BaaYo 11 ай бұрын

Thank you

@kashifahmad287 4 жыл бұрын

thank you mam so muchhhhh from Pakistan. now my presentation is ready for tomarow.

@oishe3988 4 жыл бұрын

Tomorrow

@rimjhimBedi 2 жыл бұрын

Thank God I've got to watch your vids. Otherwise I would have skipped these blotting related questions in exams. Thanks to you and ur amazing teaching skills that made me secure good marks.

@ph100k3 Жыл бұрын

Thank you mam...For Very Easy Way teach us❤💯🙌...Huge thanks to you and your team😍😇

@BaaYo Жыл бұрын

Thanks a lot 😊

@kanikamalhotra7220 11 ай бұрын

Thanku so much mam graduation students needs teacher like you........thanku soo much this is very helpful video ❤

@BaaYo 11 ай бұрын

Thanks a lot

@Dse629 4 жыл бұрын

Very helpful mam. Aap bhut accha samjhate ho thank you mam.

@ammarsayal 2 жыл бұрын

Madam hmesha se ap ke videos se bht help mili hn khas tor pr youtube pe jo itne fazul lectures hn un me ap ke videos ksi heere se km nhi Thank you soo much👍❤

@user-uf2xk2ts2b 8 ай бұрын

Woow no words 💯💯 sidha screen se dimag ke andr

@sreemotichatterjee4345 3 жыл бұрын

simply explained such a big concept. Thanks a lot ma'am for making the concept literally crystal clear.

@takini4918 2 жыл бұрын

Bdi aasani se concept clear ho jate hai aapki videos dekh kr. Dhanywad

@rajyagurukrupa7398 Жыл бұрын

Thank you so much for this explanation....you are truly amazing ma'am ❤️❤️ your short videos are much better than other 1 hour lactures...

@Euphoria321 Жыл бұрын

Love the way you deliver lecture❤ simple, elegant, full of basics❤ thankyou Madam!

@subhrajeetdash6226 5 жыл бұрын

Thank you mam..I am from chemical engineering and I find it useful in my work too

@priyanshipatel2484 3 жыл бұрын

Really a great explanation, it helped me a lot, thank you mam

@crazyjanis8258 Жыл бұрын

No one understood us like u,marvelous explanation😘😍

@muhammadosama1874 2 жыл бұрын

👏👏👏👏👏👏👏..u deserve best teacher award ....teacher of the KZfaq award

@noumansaeed7853 3 жыл бұрын

Very interested and beneficial lecture about southern blotting Thanks such a great work

@jyotsnasrivastava7661 3 жыл бұрын

Gjb mam scchi me aap mst batai pta h kb se pareshan the isko lekr .......... Hai aaj chain aaaya .😊😊😊😊

@ubaidullah8874 3 жыл бұрын

Love you from Pakistan Outstanding lecture May u live long

@nandunandini6722 Жыл бұрын

Wow I like it aap ka lecture very easily se samajh aa jata hai thanks for you mam

@maheklakkad9564 5 ай бұрын

Very clear concept , easy to understand, amazing way of teaching, mam thank you

@BaaYo 5 ай бұрын

Thank you. Please share and subscribe

@Sameerali-uw1tl 2 жыл бұрын

Thank you mam 😊 apke pass ye quality hai less time me apne sab kuch bta deya awesome 😎 mam

@fh19293 3 жыл бұрын

Thanx a lot for clearing this topic in such a beautifully explained manner😁😁😁...just a request, can u plz explain the topic of 'autoradiography'

@official.rajarshidutta 5 жыл бұрын

Mam, I am so glad to see your channel grow. I hope you remember me. I have been your subscriber when this channel had hundred subscribers. I pray this channel hits a million.

@BaaYo 5 жыл бұрын

Thank you Rajarshi :)

@sanju-bt8ss 3 жыл бұрын

Thank you so much mam

@PoojaSharma-qt4rq 2 жыл бұрын

You are such a great ma'am

@jawadjoya3004 8 ай бұрын

Well informed and have concept after almost two years thank you mam

@sciencetech2536 4 жыл бұрын

You so beautiful 😍apan ka dill. Gya ha ... chemistry ke sare notes yhi se dekhta hu

@knowmore2800 2 жыл бұрын

thank u mam u made me understand the concept easily and clearly.

@riyarajput4117 Жыл бұрын

Thanks from my heart . Mam you explain it in a very easy way thank you so much h❤

@husnainaziz8623 3 жыл бұрын

Bravo, never learned these topics with such clarity. U seems a born teacher mam🤗

@sumitpatel868 4 ай бұрын

Daimmmmmnn , thank you so much 🥺🥺🥺 I was so worried about genomics, you explain so well and logically ♥️🥺

@simransadhwani5176 Ай бұрын

Wonderful explanation mam... really love it my concept is fully clear now

@ubaidshaikh2153 11 ай бұрын

I just see your Vedios and write confidently in Exam. Thank you and God bless you!

@BaaYo 11 ай бұрын

Thank you

@vikrambiradar6411 2 жыл бұрын

Love you mam ... You saved my biotech exam paper from back log

@languageofplants1031 3 жыл бұрын

Mam ap bht axha smjati hain thank you soo much

@deekshasharma7725 Жыл бұрын

Thank you ma'am 🙏🏻...... Mere all doubt clear ho gye apki video se😊

@satyabratatripathy1613 4 жыл бұрын

It's amazing mam.. Really it is very useful for graduation students... Ty so much mam for clearing the concepts of us

@aniketdas7803 Жыл бұрын

Very helpful, thank you mam ❤

@babaralimalik1868 2 жыл бұрын

Best way of teaching....

@proudtobeveterinarian6717 Жыл бұрын

Mam 😊 now I became huge fan of you ...

@aishwaryawadkar2240 2 жыл бұрын

U r amazing.. The way U teach this biotech concepts is hilarious... Thankssssss alot mam.. U r best teacher 🙏🙏

@abhishekbbetageri6027 2 жыл бұрын

Huh? What is hilarious in that?? Ma'am is not cracking jokes. Btw, juz check the meaning of hilarious.

@haleemasadia852 2 жыл бұрын

Thanks yeh thanks Dil ki gehrayon se

@Pharmacy891 2 жыл бұрын

Mam very very thanks from my heart .for understood in easy of this difficult topic

@NavjotKaur-sf9ie 4 жыл бұрын

very nicely explained...Keep it up👍👏

@junaidaziz6933 10 ай бұрын

I am student of BS Microbiology And Molecular Genetics 🧬 😅 from University of Punjab Lahore Lahore. KZfaq is great teacher and this one mam too

@Maitri24 Жыл бұрын

Thank You so much dear Mam becouse It's help lot mam your teaching and explation style is simply superb hat's of you mam please keep uploading new vedio it's help lot 👍👍👍👍👍🙏🙏🙏 a bigggggg thank You dear mam

@umeshrai9323 4 жыл бұрын

Very nice explanation. I appreciate your lacture. .now i feel easy on the topic southern bloating

@sanjibbaishya4468 3 жыл бұрын

Such a cool personality... Learning made easy.

@pankajsaklani2343 5 жыл бұрын

I am eagerly waiting for every video of you and it proves to be very helpful for me, god bless u

@shreyaskulkarni5312 2 жыл бұрын

Only bcoz of this video i subscribed ur channel 🔥🔥 Keep it up.... Superr🔥🔥

@sultanmehmood5022 2 жыл бұрын

Thanx a lot, mam its so easy way explained, shall never b forgotten. I shall search for other blotting also

@Chillrelax630 Жыл бұрын

Ma'am boht Acha teach krti hn ap♡

@chintakayalpurnima1741 Жыл бұрын

Hello ma'am. I love all your videos. I am myself a Faculty in University, but I love the way u explain. Beautiful

@syedmedicose7477 2 жыл бұрын

excellent maam.... stay blessed

@researchease 4 жыл бұрын

Thanks and love from China. Simple and easy way me best explanation.

@parvinbegumaaraa2289 3 жыл бұрын

Mam, thanks a lot, I have little bit doubt, but get clarified after saw this video, one kind request, can U do the same in English for better understanding to all. Thank you mam. Nice explanation.

@ektasharma4816 2 жыл бұрын

Very good explanation mam 👌👌👌

@nanavatijayesh8143 3 жыл бұрын

Your teaching technique is wonderful and makes direct digestible by mind

@BTSARMY-sb8sh 2 жыл бұрын

Thanks a ton Ma'm I needed u U helped me so much for the preparation of my presentation. Can never thank you enough ever!! Lots of Love & Respect from Pakistan 💜💜

@siddhanathkagwade7411 Жыл бұрын

Ap log padthe bhi ho??

@ShivPrakash 5 жыл бұрын

Awesome keep doing best...👍👍👍

@BaaYo 5 жыл бұрын

Thanks Shiv Prakash 😊

@DebanganaGupta-us7kp 6 ай бұрын

You explain this basic concepts very well. Thank you ❤

@abhinavparmar7777 4 жыл бұрын

Currently I am doing MBBS and this video helped me lot in my Biochemistry subject... Thank you 😊

@ruchikumari1865 3 жыл бұрын

This video are very help for me... Thanks mam 👍

@kyns3946 2 жыл бұрын

Million thanks ma'am, you are too good, and made this easy to understand.

@nidhi878 4 жыл бұрын

Thank you so much it is really very much helpful

@kartikghode4850 2 жыл бұрын

Aap mere college ki biotechnology ki teacher hoti naa to aapke subject mai top krta tha😘😘😘

@user-mr5yt5ls1i 8 ай бұрын

Thank you ma'am excellent 👍 teaching

@VetAnilturkiya 3 жыл бұрын

Ma'am I love your teaching style help full conceptual

@rajarshisen7543 4 жыл бұрын

Mam your explanation is superb and very clear. All your videos are too good mam. I am a very big fan of your teaching.... Thank you so much mam🙏🏻🙏🏻.

@kashwiagrawal9785 Жыл бұрын

A very good and clear explanation, your video helped me a lot...thank u so much

@MrZurghamak 3 жыл бұрын

Keep helping students like this A huge fan of ur skill😍

@honeypatel1860 2 жыл бұрын

Really a great explaintion, thank you mam 🤗

@twinkle1548 2 жыл бұрын

Wow!! Ma'am very nice explanation, literally have no words to explain. Big fat thank you!!!!💕

@maheshswami5226 Жыл бұрын

Awesome teaching, awesome, outstanding, magnificent lecture by mol biology queen 👑👑👑👑👑, thank you so much ma'am 💞🙏

@BaaYo Жыл бұрын

Haha thank u so much

@PraveenKumar-bv2xe Жыл бұрын

Thank you mam for making this topic very simple and easy.

@zaminali4937 Жыл бұрын

Amazing teaching skill

@easywaytolearn5782 11 ай бұрын

It's a amazing explanation lot of ty ma'am . ❤❤❤❤

@BaaYo 11 ай бұрын

Thanks for liking

@Professorf11 4 жыл бұрын

Good job ... bhot achi samaj ati hai mam... Me Danish from Pakistan

@simmianam7721 3 ай бұрын

Awesome explanation... thank you so much ❤

@shahbaloch4814 3 жыл бұрын

The amazing excellent teaching method easly understanding style it is very helpful thanks A lot madom 😍

@pranayapatil7264 4 ай бұрын

Crystal cleared!! Thanks❤ mam

@BaaYo 4 ай бұрын

Most welcome 😊

@shikamarunara2827 Жыл бұрын

Loved ur explanation ma'am.. thanks a lot

@Rjt23 3 жыл бұрын

Really mam ur a good explainer....

@sayambabu7365 3 жыл бұрын

Best one yet 🤟🤟..

@prachijain8031 2 жыл бұрын

Very good explanation 👏👏👏👏

@Prince_sen_0001 Жыл бұрын

Best teacher ☺️ thanks dii🎉

@abeerfatima2704 9 ай бұрын

Simply outstanding

@youngcartoon7196 3 жыл бұрын

The way u explain it's very very very nice maam i m ur huge fan 🥰🥰

@ramchandrasuthar2848 5 жыл бұрын

Nice analysis mam

@toma902 Жыл бұрын

Ma'am, You are great...

@BaaYo Жыл бұрын

Thanks a lot

@muhammadnazakat9416 3 жыл бұрын

Thank you mam nice to meet you.

@mahmudulhasan-ws5jw 2 жыл бұрын

Thank you for clearing in such easy way

@Vishahaam 3 жыл бұрын

Excellent

@sanchariguha5337 4 жыл бұрын

Mam this is really helpful , can you please also give us some links on written notes , like from where we can read this . It would be very helpful . Thank you again .