STEC, or Shiga toxin-producing Escherichia coli, can cause human food poisoning and is often tested for on produce, meat, and other high-risk foods. Confirmation of STEC traditionally involves identifying specific genes associated with producing Shiga toxins, such as stx1, stx2, and eae. However, conventional culture methods for confirmation take several days and often have inconclusive results.
In contrast, digital droplet polymerase chain reaction (ddPCR) is a rapid, highly sensitive, and precise method for confirming STEC presence. If your products are tested for STEC, ddPCR offers an excellent solution for confirmation due to its high sensitivity, specificity, and quantitative capabilities. This makes it a valuable tool for food safety testing, outbreak investigation, and other applications where accurate detection of STEC is crucial.
In this 30-minute webinar, Dr. Feifei Han discusses the following:
✓ ddPCR technology and its applications
✓ The advantages of ddPCR for STEC confirmation
✓ The testing process using STEC ddPCR
✓ Sample requirements and turnaround time
✓ Result interpretation and implications