Sterile Technique

Рет қаралды 56,720

Күн бұрын

Sterile technique is a critical skill for success in numerous fields such as microbiology, molecular biology or cell biology. In this instructional video we will provide an overview of some of the key strategies of sterile technique, including personal protective equipment, setting up a clean workspace, and maintaining sterility while working.
For full written protocols, visit:
www.addgene.org/protocols/
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0:00 Intro
0:27 Proper PPE
1:11 Preparing Your Workspace
2:05 Working with Foil
2:29 Working with Glass Bottles
3:03 Metal Objects
3:50 Agar Dishes

Пікірлер: 25

@addgene Жыл бұрын

Addgene does not regularly monitor comments posted here, so we may not see your question immediately. We’d be happy to answer any questions sent to help@addgene.org as soon as possible. Please include the name of the video along with any questions so our support team can help. Thanks!

@shameeramahawattage7150 4 жыл бұрын

Thank you very much

@tonythetiger9839 4 жыл бұрын

good vid, thank you.

@jiaweizhang6066 4 жыл бұрын

Very useful video. Thanks for sharing!

@addgene 4 жыл бұрын

thanks! glad you found it useful!

@Wernerrrrr 5 жыл бұрын

Thanks!

@themadhatter196 2 жыл бұрын

Cooling sterile forceps with the plastic of a petri dish... what? You're going to have people melting plastic onto hot metal instruments...

@VLogKeluargaArsyla 3 жыл бұрын

thank You

@nargizisayeva1377 6 ай бұрын

Working in Addgene is my dream.

@cryptogeek3086 5 жыл бұрын

Wonderful illustrations of aseptic techniques used in the lab. Keep uploading the good stuff.

@botchjones1130 2 ай бұрын

Keep spreading mold (hopefully not) this person is clueless. LOOK OUT FOR YOURSELF

@richardtony1999 6 ай бұрын

love her gloves

@skmisra19 2 жыл бұрын

would a camp stove such as the msr pocket rocket be able to achieve the same effect as a bunsen burner for upward aerial bacterial draft / cone of protection?

@addgene 2 жыл бұрын

Thanks for your question! We haven't tried using this type of stove in the lab ourselves, so we cannot say directly whether it would work well in this context.

@ryandikdan 5 жыл бұрын

What a stunning actress! And such good technique!

@britoronto6628 2 жыл бұрын

1. Putting the plastic seals on the bottles in the flame as pictured will degrade them, and isn't at all necessary--the purpose of the flame isn't to burn or sterilize the bottle top, but to create an upwards air current near the open mouth of the bottle, preventing airborne contaminants from entering the bottle. 2. alcohol burns almost without heat so the metal doesn't get hot --the flame is used to dry the metal sterilized with ethanol, not heat it. Some people mistakenly heat the metal by keeping it directly in the flame of the burner (ie like when you heat a metal inoculation loop until red hot), and this will result in a burning hot metal utensil, but this is incorrect technique.

@nashvilleriveraquinio6432 Жыл бұрын

Great analysis! Do you references though? I would like to share this to our academe.

@wconrad4 4 жыл бұрын

Very helpful, thank you! I have never seen a candle jar used instead of a bunsen burner. Considering it for my lab. Why did you choose it? Where can I get one? Thanks!

@addgene 4 жыл бұрын

Thanks! Alcohol lamps, like the one featured in the video, are generally used when natural gas lines are not available in the lab. You can find them from most lab supply distributors (though depending on the company, they may be found under the name "spirit lamps").

@wconrad4 4 жыл бұрын

@@addgene Thanks!

@Pui1999_DNT 2 жыл бұрын

Hi Bi308 students😁

@ernestyusnizar5082 3 жыл бұрын

𝚂𝚑𝚎 𝚜𝚑𝚘𝚞𝚕𝚍 𝚋𝚎 𝚞𝚜𝚒𝚗𝚐 𝚊 𝚐𝚕𝚊𝚜𝚜𝚎𝚜 𝚕𝚊𝚋 🙂

@TanveerAhmad-eu8rq 4 жыл бұрын

heloo madam

@botchjones1130 2 ай бұрын

Sorry but I can’t take someone who clearly has no clue about aseptic techniques seriously when they’re trying to show others how to be aseptic in the lab/cleanroom lmao