Thanks for that extra info!

sech1243 You miss 100% of the shots that you don't take?

In regard to the last paragraph it really depends on what you want to do. You fundamentally have two options if you use the conventional CRISPR/Cas9 system (there are a lot of adaptations as well): You can introduce random mutations at a certain point in the genome or you can exchange certain parts (for instance a mutated gene for a "healthy" gene). These outcomes depend on two mechanisms in the cell: Non-homologous end joining (introduce random mutations) and homology-directed repair ("exchange" parts). Homology-directed repair is much less efficient than NHEJ and requires an additional DNA repair template to work (you introduce that as well). Sometimes you want random mutations, sometimes you want to exchange parts (I am just doing both in different experiments right now). The main concern regarding CRISPR/Cas9 technology are still so called off-target effects. Off-target effects arise when Cas9 starts to cut in undesired genomic regions as well. I have also attended a talk given by Doudna last summer and her data clearly suggests the risks of off-target effects. They do not really hinder your experiment if you work with bacteria, cell lines etc. but are really dangerous if you want to apply Cas9 to embryos/humans. Thus, we need to further develop the technology to make it safer.

From my reading: CRISPR = Find CAS9 = Cut It would be nice to have "Insert" command or something in the toolkit. Right now we're relying on "automatic repair" and stuffing lots of copies of the desired DNA so that the repair mechanism will hopefully be likely to use it. If that sounds tenuous and prone to error, well, it is.

​@@Lamarth1 To use CRISPR in this context is quite misleading. CRISPR itself is a DNA region in bacteria, which contains small pieces of DNA, which are used by Cas proteins (as RNA molecules) to destroy bacteriophages. To cut specific regions in DNA molecules, we use Cas9 and a short piece of RNA in the laboratory (comparable to those found in CRISPR regions but we usually make them ourselves) Insert - That's basically the utilisation of homology directed repair (a natural mechanism in which cells which use the second identical ("unbroken") DNA molecule to repair damages in one DNA molecule such as double-strand breaks). I find it thrilling that a lot of people are interested in CRISPR/Cas9!

We have yet to analyse these innocent girls as they grow up.

@@miskaalexia how is it 'yours' if joe was the one who said it??

Frankenstiening

Exactly.

Lmao why is this so funny 😂😂😂

🤔😂😍

🤔😂😜😉😍

I can see it.

Lmao

Its all going to be okay; don't freak out man. Just breathe. Don't let the fear, and paranoia get in.

If you think the world change fast, you are getting old ;) I know that because I think the world change fast and I'm old :(

Not enough for my definition of fast... I want to see in my life time : Mars&Moon travel for average Joe , 1h to travel half the global, cheap energy.etc

but today's battery technology is still a 50-year-old run.

What a time to be alive!

Similar to how or what "they" say when it comes to vaccine testing...

@justin miller "to do", not "do do", sorry, my mistake.

Do it

pharma would go crazy no more sick people to bill $$$$$$

@justin miller Precisely!

How do you know

💯 Percent true!!!

@@nokites I love your naivete. Have you ever disobeyed anyone? It's that simple. Especially if what you believe in is so strong that the end justifies the means.

MyTwoCents my question was to him how did he know and if he did then I would like evidence. Simple. If he had evidence of this then I would be satisfied but I doubt he does. I’m not opposed to the idea that there have been other clones.

@@nokites he doesn't have evidence. However we didn't have evidence of MK Ultra at one time either. 🤔

Right, the "punch" you don't see coming is the one that puts you down!!

Wat

Yep

Can we call it 'The Iceberg Syndrome' ?

@@ronschlorff7089 well, since we’re all so clever I’ll drop one too... If you heard the shot.. It wasn’t for you. Which honestly isn’t as good. Because he could’ve missed lol.

This is an abomination against Yahuah's laws!

I don't think he didn't have permission from the parents, or he would've been sued in millions by now. The question is were the parents in a position to decide for another human being

@@pardisranjbarnoiey6356 They couldn’t make the call themselves, so yes? Lots of people who don’t have the mental capacity dont make their own decisions.

@@marcdemell5976 That is not a problem that anyone has to worry about.

I find it hard to believe that in a country like China this could have been done without the governments oversight.

I’m with you Holly. Sadly we miss out on a lot! 😕

agree😭

This is exactly how I see this unfolding. The tech will not be available to the masses for disease prevention. It will only be available to the elite for enhancement.

@@mktsmith62 This is how it will go with lots of future treatment discoveries. The costs of new treatments are growing faster than the economic growth. There will not be enough money to make it available to everyone. Only to those who can afford to pay.

@@mktsmith62 this is exactly how everything goes. Every single technological advancement is expensive at first, and only a few can pay for it. Example, just to name a few, are the smartphone, GPS, microwaves, refrigerators, cars, planes, dishwashers... Every item listed here was very expensive at first, and as time went by their price lowered and the item began to be available for everyone (or almost)

Just wait. Mark of the beast. It's not a number. Put together it's the symbol of a spirochete. Singley a tail. Like the one of a pig. The one we're not supposed to eat. A corkscrew like "worm", infecting us with evil. Contaminated Forbidden Fruit. To name something in the bible Creates a responsibility or power, so it is referred to as a mark or symptom. Our doctors(modern sentinels/false prophets, white/sheep's clothing) have exploited us and our children by revealing and relabeling this spirochete as things like "autism" and "luekemia" Lues, meaning syphilis, a coincidence? No. They control our health and wealth. Suffer the little children indeed. The iniquities of our fathers (third and 4th generations) are now presenting as "autism" and spectrum disorder. Gen Z. The end of the alphabet/ life. Read the characteristics of the Goetia and you will see congruences personality and interests(spectrums), children being born without souls or Valak(demon). Fallen angels(demons) riding in on the double headed dragon. If our doctors, the Golden Rod, do not start treating us preventatively then we will be overtaken. Whats different now compared to 20 years ago? Dating sites, exposure access infedility and 1 in 50 children with autism. In the bible if you were blemished or unclean (spirochetes) you were cast out of town and no one was allowed to eat or drink or associate with you. Contamination. It is a pathogen. Jesus infected himself (gave his blood) with the spirochetes/devil/goetia to show everyone what would happen, how to recognize it when you looked on his mangled and deformed body. To save everyone else, if they were aware of it. And him. I am a worm, not a man. How do you know you have syphilis? A foul and loathsome sore. Rashes on hands and feet. Causes inflammation (fire) in the body. Why did you have to test for syphilis before marriage back in the day? Number one cause of disease and defects. Purity. Not contaminated. Our doctors figured out is causes every th ing and stopped telling us about it. The TRUE test is dna testing or PCR(covid test) we are being tracked. Covid was just another front just as autism and leukemia and any other disease. $$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$why do you think china is gene harvesting from pregnant women. To treat us or prepare for mass genocide of "contaminants"?

@@mktsmith62 "Vaccines/antibiotics/cars/air travel will never be available to common people"

Yes, my child has a non curable genetic disease and Crisper gives us hope.

Sounds terrible, best wishes to your child! As a stem cell scientist, I am not an expert in CF research but have just read some publications about the development of several new approaches to treat CF. I'll let you know if sth turns out to be promising!

I have a friend with CF, it actually stresses me out a lot because it made me have existential anxiety about her. She is one of the best human beings I’ve ever met, even though she grew up with poor health and a complex family life, and I’m happy that we can do something about CF so people with it can function much better than they could before, so having something that can make it even better is really exciting to me

St0rm Ranger it doesn’t have to be outlawed, just there needs to be a better separation of church and everything. They say there is a separation of Church and State but so many issue-stances politicians have utilize religion as a catering plate. We need a better science system in place to promote critical thinking instead of having our kids just go to school to get test scores

@@TaiWanWaf Now that you've said it, I had to read a review article about current CF trials - actually looks quite good! If youre interested, I could make a small video on my channel about this topic (Im always discussing current research in biomedicine)! Thats the name of the article: Cooney, A., McCray, P., & Sinn, P. (2018). Cystic fibrosis gene therapy: Looking back, looking forward. Genes, 9(11), 538.

Also one question please. Would this technology work the same with things like cancer?

Future humans: 👽👽👽👽👽 👽👽👽👽 I think we went to far.

It's not that simple. When we gain an immunity from evolution this doesn't always entail the risk of complications in what amounts to a medical intervention. It's more like: Born with immunity or gain it: Nature: This is fine. Humans: This is fine, but you may get cancer, live a shit life, and suffer if we fuck up, do you like gambling? The risk that these kids would actually get HIV for example may have been less than the risk that the treatment would kill them. It's also not known if this is a heritable trait. It's also not known what all the potential adverse consequences are.

Sometimes a "condition" becomes discovered to be immunity to a particular a disease.... like sickle cell anemia is a condition that prevents death from malaria. It may be also be a condition to prevent death from a disease NOT SEEN YET. That is why genetic diversity is the best situation for human (species) survival. We go and "fix" all the conditions we know about, only to be setting ourselves up to become wiped out by something unforeseen.... coming down the pipe.

@@pheresy1367 Yes, exactly! Evolution is extremely powerful. For 3.7 billion years, evolution has done us well. Meanwhile, humans have not comprehensively learned our genetics/epigenetics nor how our brain works, let alone how to edit our genes with sufficient precision to mitigate genetic errors. In the meantime, I trust evolution a hell of a lot more than I trust humans, especially those humans who aren't willing to conduct their genetic experiments with adequate supervision.

Natural immunity causes disease to less overall. Man made immunity usually extents the disease, like flu shot keeping the Spanish flu alive to this day.

I understand that. How would you feel if your children or grandchildren were adversely affected as A result? What would happen if their bodies produced to much insulin.. Food for thought.

@@midlifekrisis9060 It'll be fine just crispr them

@@midlifekrisis9060 I can't have children so it wouldn't affect anyone but me, and the reason I can't is diabetes so you understand my hatred of this illness.

I too have type 1 diabetes and would without a doubt do the same

Astraea Star oh I'm sorry Stratospheric aerosol injections..chemtrails are the conspiracy theorist word for em. John Brennan at the CFR said they are doing it. When the people doing the shit are telling you they are doing it and you still deny it. You my friend you are institutionalized. The propaganda is Workin. Look into yourself instead of believing what you hear. They are spraying something

Need a hand held tool. Time to invent. Go get at it.

Let me guess, the incriminating part?

Thinking like that, it's obvious you are autistic. It's obvious that autism is a horrible, debilitating disease that brings suffering to the life of the diseased and everyone around them. It's moronic to suggest we shouldn't treat it because it "brings diversity"

I like this sentiment.

If we are hearing about it it has been used for some time by the Elites in secret locations.

No it’s not it’s like holding a stop sign and waving them through behind your back

I like your profile picture, gunship is awesome

@@calvinabbott6920 So the Trump kids are the result of genetic engineering? Khan is not impressed.

He was sentenced to 3yrs jail in 2018 for "illegal medical practices." Official story at least.

@@ctdieselnut Jack Ma enters the room. Then disappears.

And the billionaires...

"Society free of HIV? DISGUSTING!! More diseases please" - dumbass scientists

@@theofficialdislikebutton6766 False characterization of events. He Jiankui tampered with genetic information in an imprecise way. The goal was to make the offspring more resiliant to HIV albeit with the risk of being more prone to other diseases. The entire experiment was unethical, from the lack of transparency to the goal itself.

Seriously! Like the Nazi medical experiments.Evil shit, but some useful information that couldn't be obtained ethically.

Joe hit the nail on the head. One problem is that it will be years to find out the consequences. So I guess the words here are patience and caution.

Let them allow to cure mutations, its all about happiness, natural selection will do it's job anyways 🤔

If it were done perfectly without failure, then it isn't such a problem. I still think the process is not perfect and DNA is fragile, the chances of having all you DNA damaged is probably certain. I wouldn't want that. Ya we gave you this worthless trait and all you DNA is probably as damaged as if your embryo were eradiated.

@@mykofreder1682 Im not sure about that number, but the body makes 12000 errors in copying the dna every time a new cell is created. Changing a small sequence is not a problem as long as "the scalpel doesnt slip"

@@mykofreder1682 As far as I'm concerned the father was HIV+. The transmission from father to child is comparatively very rare but this might explain why the parents agreed to participate in this study in the first place.

@@salzstangl Due to repair mechanisms in the cell, it is about 100 errors per cell division (in humans) - but I agree, bigger deletions and insertions are mostly more tragic than point mutations

I'm all for Gene editing, but only after we know what the full DNA sequence does. It's is too risky to just go into this Willy Nellie and hope for the best. Like with new drugs there is a lengthy process to be approved for human trails. Understand how this will effect the body, then start human trials and see if it works as predicted. This guy just skipped to the end, and nobody know the full consequences of his actions.

I was going to make a similar comment but figured somebody had beat me to it. I think Gattaca illustrates the most likely scenario of continued human genetic engineering. Unless we can find a way to genetically remove fear, prejudice, selfishness and greed I suspect humans will always find a way to stratify society and subjugate the less powerful.

That movie was so terrible, tho. If you had a child or loved one who suffers with a genetic disease then you would not be concerned about B SciFi movie writers' paranoid delusions.

@@chriswood232 Except they don't use genetic engineering in Gattica. They actually just use IVF and DNA-analysis to pick the "best" offspring for a pair of parents. The technology needed to realize Gattica is already well established! Scary when you think about it, isn't it?

Underrated movie right there

I deleted my comment after seeing yours. Gattaca is the logical conclusion of doing this to people.

God certainly won't. This bus has no driver, so someone better take the wheel.

@@johnathanmartin1504 Precisely. 😉

Morgan Freeman.

@@Malroth00Returns 😮 You're right!!!

Exactly God is certainly doing a bad job!

Taran Alexandre The Great INTERESTING!! I didn’t think of that.

Infant circumcision.

@@gregorymalchuk272 eeew

@@gregorymalchuk272 Yeah, that's really wrong and needs to stop!

Oh noo!

🤣

Let them allow to cure mutations, its all about happiness, natural selection will do it's job anyways 🤔

🤣😂🤣

I always thought the name of that movie was, like, the name of a place or something in it. But, with you capitalizing every letter, it makes me think of DNA, like a string of genes, with the A, C, G, T thing, you know? I've never seen the movie, btw. Wait, it is a movie, right? Not, like, a TV show? Sorry I'm so ignorant about GATTACA lol

I'm low-key concerned somebody gonna make a 'master race' . Humanity doesn't have the best history with eugenics lol. But like all tech there will be good and bad too it.

apparently @Joe Scott doesn't have enough trek fans....

Glad I'm not the only one screaming at my screen "DID YOU LEARN NOTHING FROM TREK"

@Steven Gulie I could ask for nothing but the quality of cordova's workmanship. :-D

That's hella funny.

Thanks for this.

Not cured but born resistant to hiv

Jour Namehere I’m pretty sure he said in the video that someone was cured of AIDS. He was not referring to the twins when he said it. If I have time to go back and watch this again I’ll put in the time stamp.

@@inomad1313 you got that time stamp?

ih8TrumpTardzz 4:46

There've been two of them, but none of them had AIDS, but HIV. It's not the same thing.

me too but less than a decade earlier. i was among the first babies and then i was also born premature. i wonder what your experience was like, because i was born in a very catholic town. i wasn't allowed to attend catechism because a lot of parents thought i was a fake child.

@Neocaron So you wouldn't exist if not IVF you think? How naive.

azrael, that's the whole point of in vitro

Your mother was a testube and your daddy was a knife Ps. I'm not making fun of you I just really wanted to use that quote since I'll likely will be never able to in the real world

@@azatmingalimov that is literally the only reason people spend the incredibly large amount of money it takes to perform the procedure*! If his parents were capable of conceiving naturally they would have done so. What part are you too dense to get? ... Unless you were making some pseudo-scientific woo filled claim, or worse a completely unscientific argument based in some outdated crackpot religion, in which case it is you who are hopelessly naive. I sure hope you aren't an anti-vaxxer to boot....

Lovely! And still soooooo creepy........

@@claudiamiller7730 hope you got the reference 😉

The light that burns twice as bright lasts for half as long

Yes!

@Fábio Duarte Not going to happen. Humans like to imagine themselves to be that powerful. While in reality they're just so ignorant they can't see how weak and wrong they are.

Altered Carbon on netflix does it better than gattaca.

In Gattaca anyone can do it if they have the money

Gattaca has a stupid premise (SPOILER) glorifying the stupid decision by parents who had safe option to cure their sons heart condition. It's really a no brainer that when we understand genetics well enough, we should take over the wheel. The other option is we stay on a rollercoaster of diseases and unnecessary suffering.

then have you donate yourself as their lab rat to speed up the process? ...what? no?? as expected, everything sounds good, until you become the victim

I would volunteer, my life is a living hell from a genetic disease. Death isn't always the worse thing that can happen to you. Can't possibly make it worse than it currently is.

Unless the edits are free or assigned by chance, it's likely to create an upper class of rich superhumans. The rift in society would become very literal and insurmountable. Going slow is probably a good idea, like not allowing unnecessary upgrades before every human is cured, upgrading from the bottom up, etc. Anything to keep the differences small.

Or with any other technology ever invented. 'Oh no, cars will kill us all!' People are afraid of change. But I'm suprised how open minded Joes community is. Lot's of positive comments.

@@electronresonator8882 If there was anyway that i could do so to benefit science, yes. I have several major health issues that gene therapy could probably fix and have suffered all my life. If i could die and it would cause others to not have to deal with what i have i would gladly do so. As it is when i do die my will states that my body should be donated to medical research(at osu specifically) if it is possible. Not sure where you get the idea that people or myself in particular would oppose testing done if it included our own bodies but you are very wrong.

really? none of you think it would be cool to have just a few monsters? One day you realize that Kerry down the road isn't just f&*k ugly, but is in fact a real life troll, and it suddenly dawns on you, S*!t man! that's what happened to all Mrs Sanchez's cats! I recon you could get some decent mileage out of that story.

Try drawing a portrait of an attractive human. You'll end up drawing 1000 monstrosities before you succeed.

@zach gilmore Not trying to comfort anyone. Just stating the facts, lol.

@zach gilmore I get it... It's kind of a serious subject tho ha ha

I think it's just life. We create both beauty and monsters just like we have been creating within our current human population. Better that way. Maybe we come up with some way to control the monsters. Or kill the monsters in-vitro. ¯\_(ツ)_/¯

True but that was in the 1700s, before we knew what the consequences of scientific ethics could be and built a system around it.

6:35 shoot crap my tally pod 🤔🥴😆 Did I just hear that right?😂

We are far beyond blindly. They knew exactly what it would do. Its like going to work, you may step into dogsh*, but you know where youre going to get.

well it sounds good and all, until you become the victim, these DNA scientist do not engineer their own DNA, because they want to be free of that consequences

@Electron Resonator That does not seem to be an available option yet. Based on the reporting, they can't effectively modify beyond the single cell stage yet. For all we know, they might be happy to modify themselves.

@@Lexekon Yeah, I'm sure the guy who created the small pox vaccine was so sure of it he just wanted to use someone else's child to do it just to impress everyone with how sure he was Had nothing to do with the fact that up until that point his studies were just conjecture and that he wasn't sure it would work so he didn't want to mess his life up as a result

@ spa man or the idea was that someone elses child was a more valid subject for the vaccine. I don't know if he had already survived the disease himself, but if so, he would not be a valid test subject. We cannot assume details either way.

To be effective the genetic modification really needs to be done at the single cell stage.

Oh my god guurll how much did it cost to give your baby a gucci birthmark?

Basically?

@@TheShiftingSounds Nope. Basicary, like f*ckary or bribery. You know... basicary. Or is that not a werdh??!

@Chally wally Woo Charrwy wharrwy woo*

if we think about it, natural selection has low key been doing the same

Or at least 16 colour receptors like some animals.

Found the Cylon!

You won't. You're already born.

when we have next generation smart glasses we'll be able to superimpose different spectrums onto the glasses in effect, seeing other light waves.... i want it, so i can 100% without a doubt.... *_make sure there is no bugs in my room_*

You'll be able to do that with contact lenses in a few years.

who's ethics?

Courage and Insanity are not mutually exclusive things. Lets split the difference and call him "reckless". Nearly every famous explorer was reckless in some way or another. I think the only significant difference is that explorers risk the lives of themselves and other consenting adults.

If pLaying God is brave...

Nope. When will we learn, for something so significant, a process is needed that involves humanity and NOT some vain individual wanting recognition. The people who created CRISPR-Cas9 knew not to try something so irresponsible.

@@derekgassen8460 luckily we have laws and good scientist who will think this through carefully. Maybe we should consider preserving our current DNA just in case we mess up, at least we will have a copy of our original DNA to go back to just in case we go wrong.

@@softan terrible destructive inherited genes are common in nature, especially in the human race since we force them to survive as much as possible.

Hep-C is curable. HIV is not.

Zanshin 🤣

LMAO

Sihle R 🇿🇦🇿🇦💕💕

Thanks man!

Likwise!

bc they're scientists and can grow an ear on the back of a mouse the Drs think they are close to Godliness! horrorweird

Thanks!

David Buschhorn that’s terrifying!

That's amazing

@@angelacole1270 My instructors were seriously worried about someone tweaking smallpox to wipe out an ethnic group. And it would just look like an accidental genocide. :(

Thats actually true. One girl wasn´t fully altered.

Fun fact: A small percentage of people is actually resistant to HIV due to certain mutations (I guess this gave the researcher some inspiration). I don't know if this sounds interesting to you, but otherwise I could make an episode about beneficial mutations in humans on my channel - sounds quite cool now that I'm thinking about it

Yup, I carry 2 copies of the ccr-5 delta 32 mutation that gives me functional immunity to HIV. About 10% of people of European decent carry 1 copy and about 1% carry 2 copies.

@@MissMTurner That's awesome!

@@Sciencerely yeah it's kinda neat having a literal mutant superpower!

there are diseases like malaria which are much easier to be infected with and kill more people each year

I escaped 2020 just to tell you... you had no idea, really.

I actually prefer conspiracy theory and religious nuts to those comments that are quirky "jokes" that are absolutely not funny. At least the conspiracy comments are entertaining

So you're saying that it's an intellectual channel but the people in the comments that you disagree with must just be stupid or something , Maybe they're intellectuals as well And just don't blindly believe things like you do

@@williamkelly5689 you referred to the entire population of Seattle as being ‘generally incompetent’ and take issue with my fairly innocuous comment?