True I've seen it. Whats your success on salvaging it

..or even a HeNe laser from an old style laser disk player. Not to mention some terrific optical components - X/Y servo-mirrors, birefringent crystals,...

^ no idea what kind of condition they were in but ive seen several thousand dollar machines tossed away in the back of lab buildings at universities before

Needle dumpsters….

Or just a big biohazard symbol painted on it

Haha most accurate comment on this video.

I'm changing my whole career path to become a bio teacher just so I can do number 2.

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Russell, that looks very interesting indeed, but can it be worked up into a Nanodrop replacement? Or alternatively, can it be turned into a 1 cm path length lab bench instrument. And just how good is the IR response ... good enough to take the place of a FTIR benchtop machine?

Nitril gloves are mostly black, and I love it more than latex, so I mostly use black gloves and IMMA NOT MADD!

Thom lol it was a joke! On TV shows movies and cartoons good scientist almost always have white or blue gloves (occasionally a different color) but mad scientist always have black gloves. Its one of the hints that they give. But tho protest too much, lol besides all biologist have to be a little crazy or they wouldn't be making all the crap they do. Granted you don't have to be mad to be crazy, because mad is both angry and crazy. However you do have to be a little crazy in order to make viruses and bacteria and all sorts of strange animals like a Tyrannosaurus bee lol, but it's ok anyone that isn't a little crazy is in denial about the way the world is and that makes them either a lot crazy or just plain stupid. Oh and if you should ever make some sort of Tyrannosaurus bee or any other cool animal like that I want to see it!!!

​@@curtisrobertson6069 I know it was a joke my friend. :) Geniuses were called crazy a many-many times. :D

As a biotechnology student I can tell you, black nitrile gloves are the best lmfao. Gives you a serial killer look in a way and they are better. The blue gloves are often shit. They break real easy.

@@smileyjackflanagan6053 the black nitrile gloves are thicker iirc. I'm an aircraft mechanic and I prefer those because they seem to not tear as easily which is good when you dont want hydraulic fluid or engine oil covering your hands.

if they want to do biology this is the minimum of what they'll want. Doing bio at a level where you can publish your work should be what everyone shoots for. But that doesn't mean you need the most fancy equipment.

​@@thethoughtemporium I will pay for a manual on how to raise someone like you (don't take it for granted, it is dark out there).

It's boiling on top of a lit flame and getting scattered by the hot gas, how can anything survive that?

The tiny droplet residue in the loop will pop scattering live microbes over several feet. The technique in my post is good standard microbiological technique. I've done work in graduate level microbiology and know the proper procedure. Check any lab text for the technique on flaming an inoculation loop.

^

Abhay Sharma It’s more agriculture knowledge. Possibly GMOs, but mainly how we grow plants, aeroponics is the way of the future

Aravind Karthigeyan i think u mean hydroponics?

How has it been going? Have you started any projects or amything?

@@austinkunch710 I had a huge set back after deciding to help my mother out. She ended up robing me. The debt put me on the streets. But I'm getting back on my feet and have a small setup now. I should be able to try some projects within the next three months.

@@falanirave9062 Could you message me on Instagram @austinkunch and keep in touch on how things are going?

@@falanirave9062 how's it going?

ya. it can be tricky, but the name of the technique is "around the world pcr" or "whole plasmid PCR"

Autoclave it, and set up pickup with a biohazard waste conpany

That'll either be next week or the week after! Stay tuned

connects to the igniter so it doesn't get lost. This has been replaced with one of those nice click ignition heads so the chain is gone now.

there are levels to this. A basic "dream" lab costs 50k. A *fully* stocked one costs 300-500k, ultimate dream lab costs about 5-10 mil. A "fuck it, go ham" lab would be 50 mil

@@thethoughtemporium you could throw in some gas and liquid mass specs in just for funsies!

If it's biological waste I autoclave it and then it's safe to dispose of normally. If it's chemical waste, you find your local chemical drop off location and what sorts of waste they'll take

@@thethoughtemporium Wow, thanks. It didn't cross my mind that one needs to autoclave the non-chemicals. I'm new to the hobby :) Thank you.

Just go with one of the ~$200 OMAX ones. They're all about the same and work well enough for most things. The only time I'd get something different was if I needed a dissection scope rather than one with more magnification. Anything beyond these gets expensive very fast which while nice, is often massive overkill. So there's no point in getting them unless you actually have a need.

I think you can use a spectrophotometer instead of a nanodrop sometimes

@@austinkunch710 a tiny screen that gets your reading wasting only the tinist spec of media is nice tho. you can use gels to measure DNA if you make your own ladder too (accurate as long as a portion of the ladder is the same/near your sample; you essentially take each band of the ladder, map the concentration to the intensity on the image, and graph the relationship between DNA concentration and band intensity), depending on what you want to do you don't even really need to do extra work and automate your gels for quantitative work. as long as you can calibrate to a known value of DNA you can use many methods.

You could DEFINITELY make that happen

Sure, you just need to be careful as some of the stuff there is crap, but some is pretty great.

if it's autoclaved and sterilized it's no problem

Just down the sink, gotta make some mutant giant sewer leeches n stuff to feed the mutant giant alligators in there

I will take a case of pippettes and tips, agarose

@@phillipnichols send me an email to brad@briscoe.online with the address you would like me to ship them to. I'll send you a picture of the cases I have. I'll assume you want a single channel auto pipette.

Etham Buchbinder hahaha... that might be a little beyond what an DIY biologist can afford. What are you trying to make? You can probably have it synthesized professionally for way cheaper(5-10 cents per bp) than trying to build your own machine.

@@tainicon4639 totally agree, BUT how cool would it be!?!?

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Daniel Mallett it would be amazing! And potentially dangerous... currently you can download a copy of the small pox genome. With a plasmid containing all of the genes you could very easily make a competent virus. But the skill required to pull that off might be beyond the diy biologist...

@@tainicon4639 I would argue that its potential for bad is only surpassed by its potential for good I do also think it is probably far outside the abilities of the DIY amateur

No

Cuz in a bio lab there is just lots of throw-away stuff

Including cost of building a lab id estimate 5k to equip it and some ongoing costs

Depends on what you need to do

Useless if you're trying to isolate a culture. You'd need to go through multiple disposables to do one proper streak plate while a metal one can just be re-sterilised as you go. The process to get to a plate with single colonies requires you to first inoculate a small region, then sterilise the loop and drag a few lines out from it, then sterilise and drag out a few more lines from the new ones you just streaked out, and so on.

So did you?

Depending on what you're doing, doing your sterile work under a Bunsen burner (or blowtorch) can suffice. The updraft generated by the high temp flame will be enough to stop any particles in the ambient air settling onto your work, then once you're done doing what you're doing, you cap it/cover it and you're fine.

It is now! It's been a dream to do this full time for years and thanks to the support of my patrons and channel members, now this is the main thing I do!

@@thethoughtemporium congrats , I love seeing your work! helps me stay motivated in university.

It is quite a bit of plastic waste

They are blue oyster mushrooms I believe

18l

Thank you!

Not really :(

The micropipette costs most out of all of these. You could use cheap microcapillary pipettes instead and Im shure you can save some bugs by building your own pcr machine.

Yeah you could

I imagine it's hard to do something like this without any degree + it's very expensive

A 15ml centrifuge and a small eppendorf centrifuge should go a long way, really all of dna extraction is usually done w a small eppendorf tube centrifuge

Thanks a ton Austin! I looked all over for this info.