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This video demonstrates characterization of a molecule or a mixture with thin-layer chromatography or TLC. The separation of molecules based on their polarity is discussed along with the roles of the stationary phase and mobile phase. In this video, the stationary phase of the TLC plate is silica gel. Different visualization methods, including UV light and iodine, are described. Uses of TLC, such as monitoring a reaction and checking purity, are also covered.