Thin layer chromatography

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Quick Biochemistry Basics

Quick Biochemistry Basics

Күн бұрын

Thin layer chromatography is based on adsorption chromatography. In this method, the adsorbent material is applied on the glass plate, plastic surface or an inert solid surface. Once the plate is ready the sample is applied at the end of the plate. The plate is then transferred in a developing chamber that contains mobile phase solvent. The solvent moves up the plate via capillary action. The sample molecules gets separated on the basis of their interaction with the mobile phase and stationary phase.

Пікірлер: 16
@omsharma3080
@omsharma3080 11 ай бұрын
Nice explanation👍Btw there are 2 more methods to detect colourless analytes: 1. By placing the chromaplate in glass chamber containing few crystals of Iodine (they adsorb iodine vapours and look as brown spots) 2. Spraying Ninhydrin reagents on chromaplate (for detection of amino acids)
@CompetitionChemistry
@CompetitionChemistry 3 ай бұрын
Thankyou so much..I will use this knowledge to explain my students 😊 thanks
@meowmeow-dw3bi
@meowmeow-dw3bi 4 ай бұрын
amazing video sir
@user-sy9gn3le6n
@user-sy9gn3le6n 11 ай бұрын
Nice and clear explanation tq superb
@user-gy3zm6du7s
@user-gy3zm6du7s 2 ай бұрын
Thankuuuu so much 😊
@NitishKumar-oy2lk
@NitishKumar-oy2lk Жыл бұрын
Hello sir I want biochemistry notes can you please provide me
@Aestheticsanatanvideos788
@Aestheticsanatanvideos788 11 ай бұрын
S ofcourse
@cupofcalculus
@cupofcalculus 11 ай бұрын
There is no "d" in "absorbent".
@uniqueen1367
@uniqueen1367 7 ай бұрын
It's not absorbing it's adsorbing which are different
@shrijeetkolley9440
@shrijeetkolley9440 6 ай бұрын
Lemme guess, American?
@princeofninese6431
@princeofninese6431 6 ай бұрын
​@@shrijeetkolley9440hello mate, I too am watching
@Serwada237
@Serwada237 5 ай бұрын
Adsorption and absorption are different
@samisplaying2527
@samisplaying2527 5 ай бұрын
It is actually "Adsorbing"
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