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Triple Sugar-Iron Agar is used for the determination of carbohydrate fermentation and hydrogen sulfide production in the identification of gram-negative bacilli.
Triple Sugar-Iron Agar contains three sugars (glucose, lactose and sucrose), phenol red for detecting carbohydrate fermentation and ferrous ammonium sulfate for detection of hydrogen sulfide production.
Carbohydrate fermentation is detected by the presence of gas and a visible color change (from red to yellow) of the pH indicator, phenol red. The production of hydrogen sulfide is indicated by the presence of a precipitate that blackens the medium in the butt of the tube.