Vitamin-A & Vitamin-E Analysis Using HPLC_Part-2 (Instrumental Analysis)

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Күн бұрын

Vitamin-A and Vitamin-E are most common among the fat-soluble vitamins. Quantitative determination of Vitamin-A and Vitamin-E in food are the most important test parameters, especially for food samples. Quantification of the Vitamin-A and Vitamin-E using HPLC is the most precise and widely used method. Instrumental analysis is very important for the interpretation of the test results. This video represents a detailed procedure of the instrumental analysis for the determination of Vitamin-A & Vitamin-E using HPLC.
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Sample Preparation for Vitamin A & E Analysis: • Vitamin-A, Vitamin-E &...
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Пікірлер: 91

@neerajpandey2650 Ай бұрын

what an video on vitamin, Great video and very clean each step and very knowledgably,

@talesdemidioful Жыл бұрын

Thank you a lot, you deserved a medal

@MicroChemsExperiments Жыл бұрын

Thanks. I just received one medal from you.

@aswinkumar8016 3 жыл бұрын

Thank you... Most awaited video...

@MicroChemsExperiments 3 жыл бұрын

You are most welcome. Support us by sharing this channel.

@ALAMIN-ik8ph 2 жыл бұрын

Both are very informative & helpful video.

@MicroChemsExperiments 2 жыл бұрын

Glad you think so!

@chuongph8115 2 жыл бұрын

Thanks you, i like channel, you are so professional

@MicroChemsExperiments 2 жыл бұрын

Thank you

@zenaidadelacruz6048 11 ай бұрын

Thank you so much for this very detailed and informative video. This is a great help. Also would like to ask if you have video for analysis of water soluble vitamins.

@MicroChemsExperiments 11 ай бұрын

Coming soon!

@kendyangue3678 6 ай бұрын

For Vitamin D analysis using hplc instrumental analysis?

@ujjwolsubedi9603 2 жыл бұрын

Thank you so much for such informative content. Can you please provide the reference source for this protocol?

@MicroChemsExperiments 2 жыл бұрын

No specific reference. This is a in-house developed method

@ujjwolsubedi3683 2 жыл бұрын

if we want to publish work with this protocol how can we cite the reference for it?

@amornratrat 4 ай бұрын

Why stock solution Vitamin A or E is made saponification ? almost standard prepare dilute solvent already to inject.

@Jithinjoyv07 2 жыл бұрын

Hi could you please share which method like iso or AOAC like?

@MicroChemsExperiments 2 жыл бұрын

Sorry. We don't provide any document

@chemmuhammad8471 2 жыл бұрын

is the preparation method for vitamin D standard the same as vitamin A and E and what is the wavelength in which we detect vitamin D and is calculations the same also?

@MicroChemsExperiments 2 жыл бұрын

Sample preparation is same but instrumental analysis is different. We will upload instrumental condition later. Stay with us.

@chemmuhammad8471 2 жыл бұрын

@@MicroChemsExperiments thanks

@MicroChemsExperiments 2 жыл бұрын

@@chemmuhammad8471 Anytime

@aswinkumar8016 2 жыл бұрын

I have been trying this method for Vitamin A I am not getting the result exactly for curd...

@MicroChemsExperiments 2 жыл бұрын

Sorry for your situation. Try to follow this method strictly. Saponify the sample sample and the standard properly in dark.

@tootahashemy5427 2 жыл бұрын

Its important to filterd methanol

@MicroChemsExperiments 2 жыл бұрын

Yes. It is very important

@velimattiollilainen Жыл бұрын

I would recommed HPLC grade solvents, and the use of fluorescence detection (for vit E exition 280 and emission 325nm, vit A ex 342 nm, em 476 nm) to get much better selectivity and sensitivity.

@epalanisamiepalanisami6863 2 жыл бұрын

Can we use methanol as a solvent in the place of petroleum ether in Gas chromotograp?

@MicroChemsExperiments 2 жыл бұрын

Yes. You can

@epalanisamiepalanisami6863 2 жыл бұрын

@@MicroChemsExperiments Thank u

@rajuahmmed1257 10 ай бұрын

Which hplc model used for this test? Please share the standards preparation link

@MicroChemsExperiments 10 ай бұрын

Model: 3000 Ultimate. We will make video for standard preparation later

@Nicha-qu3nw Жыл бұрын

This extracted sample preparation method can use all sample or specific ex. snack fruit vegetables

@MicroChemsExperiments Жыл бұрын

All samples

@Nicha-qu3nw Жыл бұрын

Can you share the range of std curve that you used .

@MicroChemsExperiments Жыл бұрын

@@Nicha-qu3nw 5 10 20 50 ppm

@mdalaminhossen6857 3 жыл бұрын

How to test the gamma origin of Reich Bran oil sir

@MicroChemsExperiments 3 жыл бұрын

Do you mean Gamma Oryzonal?

@tnehshuxin9530 9 ай бұрын

Sorry I'm not understand about the standard preparation. Can you explain it?

@MicroChemsExperiments 9 ай бұрын

You can mail us explaining the problem. Write us at mail.mic.chem@gmail.com

@aymenogkush 3 жыл бұрын

Hi, first thank you for the video. Can you give me the reference of the standards for Vitamin A and E so that I can buy it

@MicroChemsExperiments 3 жыл бұрын

Buy from Sigma Aldrich

@aymenogkush 3 жыл бұрын

@@MicroChemsExperiments yes I know its from Sigma Aldrich, but I need the product number because when I make a search for the vitamin, it gave me several ones, and I don't know which one should I choose

@MicroChemsExperiments 3 жыл бұрын

@@aymenogkush 1. Vitamin-A standard: (Retinyl Acetate analytical standard; CAS Number=127-47-9; Product Code: 46958) 2. Vitamin-E (Alpha Tocopherol analytical standard; CAS Number: 10191-41-0; Product Code: 47783)

@aymenogkush 3 жыл бұрын

Thank you very much. And for the filter that you used for the filtration of the methanol, what is the type of the material (PTFE or Cellulose or Nirile or Glass fiber etc..) and the porosity that should I used? If it's possible the product number

@MicroChemsExperiments 3 жыл бұрын

@@aymenogkush Cellulose membrane filter. Look up to the video. Details specification is seen when using the filter peper in this video. Product number is not avaulable for this product. You can purchase this from the local market.

@rajuahmmed1257 3 жыл бұрын

Waiting

@MicroChemsExperiments 3 жыл бұрын

What are you waiting for?

@rajuahmmed1257 3 жыл бұрын

@@MicroChemsExperiments For more experimental video. Specially for hplc

@MicroChemsExperiments 3 жыл бұрын

Thank you for your patience. We will be here with many more videos about HPLC. Stay with us. Share this group to support us.

@rajuahmmed1257 3 жыл бұрын

@@MicroChemsExperiments the videos you provided are really fantastic. It attracted my attention due to Covid-19. I was share your Videos to my students.

@MicroChemsExperiments 3 жыл бұрын

@@rajuahmmed1257 Thank you so much for your support.

@HishaniPrashar 11 ай бұрын

please provide informatio, How should we prepare stock solution, for Vitamin A

@MicroChemsExperiments 11 ай бұрын

Saponify your standard and make the final volume of 10 or 100 ml with methanol. For more details, mail us

@HishaniPrashar 11 ай бұрын

@@MicroChemsExperiments Thank you

@omarsayed2458 2 жыл бұрын

Why do you set run at 30 min while RT of vitamin E is about 8 min

@MicroChemsExperiments 2 жыл бұрын

For proper column washing. You can set the run time at 20 min.

@mahmoudkamel4181 Жыл бұрын

Hello, Instrumental analysis for vitamin D?

@MicroChemsExperiments Жыл бұрын

Noted

@kendyangue3678 6 ай бұрын

Where is the video for instrumental analysis of vitamin d?

@gauravkamble4085 Жыл бұрын

Where you buy this standard Vitamin ?

@MicroChemsExperiments Жыл бұрын

You can buy standard from Sigma Aldrich

@gauravkamble4085 Жыл бұрын

@@MicroChemsExperiments and please tell me about the vitamin b sample presentation

@MicroChemsExperiments Жыл бұрын

@@gauravkamble4085 we will make video later

@aswinkumar8016 2 жыл бұрын

What is the column your using here please mention that

@MicroChemsExperiments 2 жыл бұрын

Watch this video again. All the details is given in this video

@aswinkumar8016 2 жыл бұрын

@@MicroChemsExperiments I am using hypersil gold column

@MicroChemsExperiments 2 жыл бұрын

@@aswinkumar8016 Did you get the vitamin A standard peak?

@aswinkumar8016 2 жыл бұрын

@@MicroChemsExperiments Yes, but the value is very high when compared to the label value

@aswinkumar8016 2 жыл бұрын

That's why I am asking ur using normal hypersil gold column or acclaim 120 PA / C18 column

@user-sb8lp4sf3g 2 ай бұрын

I want to say calculation method for viramin A and E by HPLC

@MicroChemsExperiments 2 ай бұрын

We don't know about the method you are talking about.

@user-sb8lp4sf3g 2 ай бұрын

@@MicroChemsExperiments How can we calculate the content of Vitamin A and E in our Sample?

@user-sb8lp4sf3g 2 ай бұрын

I mean ....Please share calculation formula

@user-sb8lp4sf3g 2 ай бұрын

please share calculation formula.....I'm waiting

@valeedck8738 9 ай бұрын

Where is vitamin d analysis procedure?

@MicroChemsExperiments 9 ай бұрын

Will upload later

@vsolomtn Жыл бұрын

Filtering MeOH, sonicate it and then putting inside a piece of cork, bad techniques and sources of contamination are dressed here as an expert advice in just 3 min.

@vsolomtn Жыл бұрын

I kept watching the video and now I understand that peak tailing, you're destroying your instrument, mister. Worst of it all is that you must be teaching people both in real life and through your videos, I'm sorry for them.

@velimattiollilainen Жыл бұрын

@@vsolomtn peak tailing makes more variation in the results as the proper integration of tailing peaks is not easy

@vsolomtn Жыл бұрын

@@velimattiollilainen Peak tailing is caused by different migration speeds of the analite inside the column, which indicates a poor performance of the system. Areas indeed will be different because because that method isn't suitable for any kind of separation. It can't be a matter of playing the edges on a latter integration when you have no reproducibility to start with.

@MicroChemsExperiments Жыл бұрын

Noted