What is Fluorescence Recovery After Photobleaching (FRAP)?

Рет қаралды 9,925

Күн бұрын

FRAP is a microscopy technique that scientists use to determine the mobility of proteins inside living cells. In this video, Jayme Dyer explains how FRAP works, and how scientists used FRAP to better understand how cells crawl!
The paper discussed in this video is:
D. Raz-Ben Aroush, et al., Actin Turnover in Lamellipodial Fragments. Current Biology 27, 2963-2973.e14 (2017). doi.org/10.1016/j.cub.2017.08...
Video & Image Credits:
Video of crawling keratocyte by E. L. Barnhart, K.-C. Lee, K. Keren, A. Mogilner, J. A. Theriot, An Adhesion-Dependent Switch between Mechanisms That Determine Motile Cell Shape. PLOS Biology 9, e1001059 (2011). CC-BY
Scanning Electron Micrograph of actin in lamellipodium by T. M. Svitkina, G. G. Borisy, Arp2/3 complex and actin depolymerizing factor/cofilin in dendritic organization and treadmilling of actin filament array in lamellipodia. J Cell Biol 145, 1009-1026 (1999). CC-BY NC SA, Cell Image Library accession number 24801
Still image of GFP-labeled F-actin in neonatal cardiomyoctyes by Ps1415 CC-BY SA 4.0
Video of labeled actin in live mouse embryonic fibroblast cell from G. M. Rivera, et al., Requirement of Nck adaptors for actin dynamics and cell migration stimulated by platelet-derived growth factor B. Proc Natl Acad Sci U S A 103, 9536-9541 (2006). Cell Image Library accession number 31939
Hammock Fight music by Kevin MacLeod is licensed under a Creative Commons Attribution 4.0 license.
Extra Special Thanks to Drone Flight Engineer, Wil Weldon
Extra Special Thanks to Confocal Microscopist, Kyle Moran
Special thanks to these “protein actor” volunteers:
BRIDGET BELL
CLIFF DYER
JULIA ELMAN
JERRY G. GARFUNKEL-VARGAS
JOHN MURPHY
AMY O’REGAN
BROOKE PHILPOTT
DEREK ROWE
HENRY SOLÓRZANO
ARMAN TAHMASEB
KAMBIZ TAHMASEB
TALA TAHMASEB
BORNA ZAREIESFANDABADI
This video is copyright Jayme Dyer 2021, published under the Creative Commons CC-BY SA 4.0 license.
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Пікірлер: 17

@user-pz6zy3ji7u 11 күн бұрын

The best explanation on KZfaq! Thank you so much!

@student_remo 4 күн бұрын

Love the music and love the bird's eye view explanation!!!

@laurelcook9078 Жыл бұрын

This was the best explanation for anything I've ever asked ever.

@YouTooBio Жыл бұрын

Thanks! 🥰

@ellasermon5677 Ай бұрын

This is such a good video. It is clear that you guys know how to explain difficult htings in a way everybody can understand. Thanks!

@devonwinterer5302 Жыл бұрын

Great explanation! Thank you for this video. Science can get overwhelming sometimes...until people like you simplify it, then, it is fun again!

@YouTooBio Жыл бұрын

Glad I could help make science fun again!

@RyonBang 3 ай бұрын

This is such a good content!

@YeshvirYadav Жыл бұрын

What a legendary explanation, Diamond. Thanks

@abbygailbenitez9131 Жыл бұрын

oh my gosh this was so helpful for my cell and molecular bio class amazing job and THANK YOU SO MUCH!

@alibizri8793 Жыл бұрын

Excellent video. Thank you!

@maayangezunterman4831 5 ай бұрын

Such a good way of teaching. 10/10. I absolutely understand it now. Wish I met your channel earlier 😁

@michellew4634 Жыл бұрын

great video! super cool

@argosyseven 3 күн бұрын

In the graph at 4:50, did you perhaps accidentally swap mobile with immobile proteins? It would seem to me that if the ROI recovers its fluorescence close to 100% before photobleaching, it would be due to diffusion of proteins

@tamilspeaker2507 10 ай бұрын

Mam can you do a video on Fret technique

@terranovarubacha5473 2 жыл бұрын

Could you go into more detail on what's happening in the sides of the fish keratin cell? If not much is known, maybe just an addendum to help define the 'tightly packed network'. Love your explanations :)

@YouTooBio 2 жыл бұрын

The sides of the cell are a lot like the back: actin is disassembled there to promote "pulling in" the sides so the cell moves forward and isn't torn in half! Actin filaments grow at one end and break down at the other end, and they can form branching structures with the help of accessory proteins (notably Arp2/3). Many, many proteins are involved in controlling how fast and where actin forms filaments! This video is just the tip of the iceberg on how actin contributes to cell crawling :)