What is silver staining and how does it work?

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Күн бұрын

Technique: Silver staining
In this video I describe how silver staining works. It is used in DNA, RNA and protein research, and it is used to stain tissues particularly the brain. So you can see it is a very diverse technique, in fact black and white photography can be seen as a form of silver stain. Silver staining relies on the process of turning a transparent solution of a silver salt (silver nitrate), into opaque metallic silver. In this video I explain how this happens and how this process can be utilized as a stain in histology and in vitro (test tube) techniques.
Learning objectives
Describe the uses for silver staining
Explain the chemistry behind silver staining
Discuss the roles of nucleic acids, acid amino acids and impregnation reagents in silver staining
Give an example of how silver staining in the brain can be used to quantify dementia

Пікірлер: 6

@sarangmt 2 жыл бұрын

Wow, Thank you brother.. was looking for just same information and mechanism.

@anjalinoklalpardhi1295 2 жыл бұрын

Such a beautiful explanation and everything was on point . It was interesting because i too love how neurons works and all the processes happens .

@kishanjilka8820 Жыл бұрын

NICE EXPLANATION THANK YOU

@user-wr8ih7dw3p 8 ай бұрын

thank you very much for this video, very helpful. I would like to ask whether the silver ions bind because the DNA is acid and so donates electrons, or because the DNA itself is normally negatively charged due to the presence of negatively charged phosphate groups all over the backbone. Thank you!