Hello Doctor! I was diagnosed with HTLV-1, I have hyperthyroidism, osteoarthritis in my spine and osteoarthritis in my knees (I had surgery on my spine and a knee replacement). Do these bone problems and hyperthyroidism come from HTLV-1? I am 64 years old and female! A hug from Brazil!
@adwoabiotech23 күн бұрын
Hello! Apologies, I'm not a doctor. I am not aware of these conditions being caused by HTLV-1, so please consult your doctor. Here's a link to a video discussing the causes of hyperthyroidism: kzfaq.info/get/bejne/nt6qfdt0tLTcoZ8.html I pray you find the underlying cause, so that you can find curative treatments. All the best!
@freeseyyah1547Ай бұрын
I want to ask a question. My English skill is not enough to understand . It could be silly question sorry . I am trying to learn statistics . I would love to do statistics of my researches my own . I want to do a research about "Mortality and complications after percutaneous endoscopic gastrostomy)PEG): a retrospective one-centered study" My universe is 908 PEG . After excluding criterias I dont know how much will stay but probably around 600 cases I want them all. How can I calculate for that retrospective research sample size. 1-After PEG minör complication rate : %18-38 major complcation %2-4 2-After PEG mortality rate in 30 days : %3-23 I want to do multiple regression analyses . I have 2 dependent variables "mortality after peg " and "complications after peg" I have 31 independent variables. I do calculate at GPower . F test- Linear multiple regression : fixed model, r2 deviation from zero: A priori Effect size 0.15 , a 0.05 B: 0.95 number of predictors 31 Sample size 264. ***My question is this how can ı find effect size? Do you suggest me any other advice for my research sample size calculate ? Thanks for helping and sorry for baby english and long question.
@adwoabiotechАй бұрын
Hi! As I am not a statistician, I cannot fully help. However, based on what I've understood of your question, you may be able to calculate the odds ratio for the percutaneous endoscopic gastrostomy vs. those without the intervention. This would give you the effect size parameter to input into the sample size calculations. For the regression analysis, perhaps a correlation coefficient would be the best way to assess it's effect. Please confirm theses approaches with a qualified statistician :)
@muffinman1Ай бұрын
Isolation of CTCs are also another popular use for SepMate/Leucosep tubes.
@adwoabiotechАй бұрын
Good to know, but what does CTC stand for in this context? Thanks.
@muffinman1Ай бұрын
@@adwoabiotech circulating tumor cells. A popular prognostic biomarker. Look into cellsearch assay.
Additional Essential Cell Culture Calculations (making up media and determining amount of drug treatment): kzfaq.info/get/bejne/ZpqpZ6edldu4cWw.html
@Wendy-pz3xnАй бұрын
Can a man be a carrier and not even know?
@adwoabiotechАй бұрын
Hi Wendy, Yes, most individuals infected with HTLV-1 remain asymptomatic carriers throughout their lives. Only, a small proportion of infected individuals develop HTLV-1-associated diseases.
@Wendy-pz3xnАй бұрын
Can this virus 🦠 cause CML?
@adwoabiotechАй бұрын
I'm not sure about this one but the two main clinical conditions associated with HTLV-1 are adult T-cell leukemia/lymphoma (ATLL) and HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP).
@nanaobben51662 ай бұрын
Excellent ❤
@slate14962 ай бұрын
Thanks
@adwoabiotech2 ай бұрын
Reference 2): Sequencing artifacts derived from a library preparation method using enzymatic fragmentation: journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0227427&type=printable
@adwoabiotech2 ай бұрын
Reference: DNA Library Preparation Methods for Next-generation Sequencing: Tone down the bias www.sciencedirect.com/science/article/abs/pii/S0014482714000160
@lloviset3 ай бұрын
Thanks!
@adwoabiotech3 ай бұрын
You're welcome, mate.
@Shiv-vs8oy3 ай бұрын
here we join the base with a line manualy which is error prone for the area calculation
@adwoabiotech2 ай бұрын
Yes, that does sound error prone.
@adwoabiotech3 ай бұрын
References: 1) High rhesus (Rh(D)) negative frequency and ethnic-group based ABO blood group distribution in Ethiopia: www.ncbi.nlm.nih.gov/pmc/articles/PMC5530478/#:~:text=In%20Africa%20and%20Asia%20the,negative%20phenotype%20is%20even%20rarer. 2) Chapter 2: Blood group antigens are surface markers on the red blood cell membrane by Dean L. Bethesda (MD): National Center for Biotechnology Information (US); 2005. www.ncbi.nlm.nih.gov/books/NBK2264/#:~:text=Blood%20group%20antigens%20are%20either,the%20transfer%20of%20sugar%20units. 3) Blood groups (Better health channel VIC health) www.betterhealth.vic.gov.au/health/conditionsandtreatments/blood-groups# 4) Blood Groups and Compatibilities www.rch.org.au/bloodtrans/about_blood_products/blood_groups_and_compatibilities/
@alhassanmohammedawal38513 ай бұрын
Hi Maame Adwoa, please i have a malaria vaccine to analyse the protein content. I have the Bradford Protein kit from thermofisher and the Albumin standards for the curve. can you help me with how to go about it?
@adwoabiotech3 ай бұрын
Hello Alhassan, You will need make a 6-point serial dilution of a protein standard for which you know the starting concentration. e.g. Bovine Serum Albumin (BSA). You will then measure the absorbance of that standard against your protein of interest, in this case, your vaccine. If you have a plate reader, it may be also be easier assay it with that instrument (provided you have enough reagent for the required plate reader vol., typically 200ul). I have a video on here, that discusses how to approach protein determination so I'll link it below.
@adwoabiotech3 ай бұрын
Standard Curve: kzfaq.info/get/bejne/g7aBp5h1ytPemoE.html
@alhassanmohammedawal38513 ай бұрын
i tried to contact them but they will not response
@kalyanirajalingham12863 ай бұрын
Very nice presentation
@adwoabiotech3 ай бұрын
Thanks :)
@aondohembanege95514 ай бұрын
Hi, thanks for the video. Please, I have a question: I will like to ask a question about cell treatment experiment with a test drug for 72 h. In such an experiment, do I have to add my drug once and incubate for 72h then check the cell viability or I have to remove the drug solution and replace with the same drug and concentration after each 24h to avoid wrong readings due to degraded drug in wells or evaporation? Please any other person reading the comments can also reply me if you have the correct answer to my question. Thank you
@adwoabiotech4 ай бұрын
The usual strategy is to leave the drug on for the entire 72hrs and assess following the designated time. However, it depends on the stability of the drug that you are assessing. If you know that your drug of interest is unstable and is completely degraded after say, 24hrs, then it may be wise to replenish.
@aondohembanege95514 ай бұрын
Thank you very much for your timely reply. The information will add to my considerations and subsequent decision @Adwoabiotech
@phuonglannguyen65914 ай бұрын
hi thank you for your explaination. I have a question, if I measure dose dependency, in specific, I measure basal value of each well for 10 mins, and then add agonist and measure for 30 mins more. Then its supposed to have many points, then how should i choose value to make Z' factor?
@rayshoesmith4 ай бұрын
I am a complete novice with ELISA but i need to run some blood tests that the UK does not offer, im going to buy all the equipment to conduct the following tests: MSH TGFB1 VEGF MMP9 C3A C4A Can you recommend a good book for the protocols and what elisa tests would be appicable for these tests (dircect/indierct etc.....) The top one is melanocyte stimulating hormone and i need to see a quantity not just sample presence in all of them Thank you, great videos
@adwoabiotech4 ай бұрын
Hi @Rayshoesmith In terms of whether you perform a direct or indirect test, this will depend on how abundant the target is. If you suspect very low expression levels, then go for indirect. Regarding the melanocyte stimulating hormone where you need to see quantity not just presence, you will need to include a 6 or more point standard curve. This will help you report quantities. Regarding a book: I found this one online: ELISA: Methods and Protocols (Methods in Molecular Biology, 1318) 2015th Edition www.amazon.com/ELISA-Methods-Protocols-Molecular-Biology/dp/1493927418 All the best!
@rayshoesmith4 ай бұрын
@@adwoabiotech you are an absolute angel! Thank you for taking the time to reply, I really appreciate you ❤️
@archanal24945 ай бұрын
How do you adjust the gain?
@minhhao50315 ай бұрын
Thank you so much for this practical lecture for university students like me who cannot have the condition and biotechnological facilities to practice. I wish you all the best in 2024, Madam!
@adwoabiotech5 ай бұрын
Glad it was helpful!
@benabuindetiemo31835 ай бұрын
does the volume of the samples have to be constant
@adwoabiotech5 ай бұрын
Without having more details about which volume you are referring to, I will still say 'yes', because volume affects concentration.
@anassoub60885 ай бұрын
Really helpful thank you !
@adwoabiotech5 ай бұрын
Happy to hear.
@adwoabiotech5 ай бұрын
Why do we need to be able to do such calculations? In order to maximize all available storage space most solutions are stored in a concentrated form (known as stock). These solutions are then diluted to the required strength as and when required. This also means the same solution substance may be used for a different range of needed concentrations.
@drtomiomoya5 ай бұрын
Thanks for the information. Love it
@adwoabiotech5 ай бұрын
You are welcome @drtomiomoya.
@user-ro5gh9ez5c6 ай бұрын
7:36
@Scotto_desu6 ай бұрын
Great video 👍
@adwoabiotech6 ай бұрын
Medaase / Thank you.
@user-zq7zb6oo1n6 ай бұрын
keep up the goodwork. I hope someone there's multiple of someone like you in this world. You keep the world turning
@axox116 ай бұрын
Thank you! ❤️
@adwoabiotech5 ай бұрын
You are welcome.
@adwoabiotech6 ай бұрын
What is a good H-index? Hirsch suggests that after 20 years of research, an h-index of 20 is good! It means that you have 20 publications with at least 20 citations. 40 is outstanding, and 60 is absolutely exceptional.
@user-ou1lk1uf6u6 ай бұрын
I analyzed your channel and I was impressed to see your video quality. your video thumbnail is very eye-catchy. However, you are not getting proper views and subscribers. Below we found some problem for your channel - Your video title is not SEO friendly Don't have proper tags and keywords Don't have meta tags for video ranking Descriptions are not optimized Please fix this problems to get more views and subscribers
@adwoabiotech5 ай бұрын
Ok thanks for letting me know 😆
@jacquimurphy43946 ай бұрын
I struggled to listen to it due to the music which is a shame as sounds like it may have been useful 😢
@adwoabiotech6 ай бұрын
The music in this video is barely audible. You may have had another browser opened, that was playing music?
@albertofabbri15436 ай бұрын
@@adwoabiotech Sorry but I do not agree with you. The music is not as low as you say and it makes harder to understand what is being said.
@adwoabiotechАй бұрын
@@albertofabbri1543 Ok, thanks. I appreciate you letting me know. I will keep it in mind for future videos.
@adwoabiotech7 ай бұрын
Other terms used to describe water quality in the lab includes: MQ/MilliQ water for type 1 water; Elix water for type 2 water and RO or demineralised water for type 3. Note that distilled water is water that is clarified through heating the water until it enters vapour form, and then cooling the water back to liquid form. Most labs do not use this method of clarification, preferring to use reverse osmosis, due to its greater efficiency.
@bastonmark16267 ай бұрын
Of course it is obvious that we do not appreciate qualities and the cost that it rather saves.
@adwoabiotech7 ай бұрын
It seems common sense once you learn it, but before then, it's not intuitive.
@Nofux2give7 ай бұрын
you saved my life man
@adwoabiotech5 ай бұрын
Glad to hear.
@daryaaleksandrova428 ай бұрын
Thank you!!
@adwoabiotech8 ай бұрын
You're welcome!
@adwoabiotech8 ай бұрын
Fun fact: a typical adult has a blood volume of approximately 5 liters. Females and males have approximately the same blood percentage by weight.
@dd20538 ай бұрын
Hi Adwoa Could you explain the interpreting Elisa result a bit more Do you have one-2-one tutorial?
Note: some labs avoid using antibiotics in their Media as they don't want to risk masking low level infection or worse, facilitate the emergence of antibiotic resistant strains. Get more comprehensive knowlege via this article from pubmed: www.ncbi.nlm.nih.gov/pmc/articles/PMC7149418/pdf/main.pdf
@enriquecorona31849 ай бұрын
PBMC will be used to assess viral infections, thanks.
I want to use artificial intelligence to come up with guide RNA sequences and target sequences in Pam sequences. but i also want a way too verify that the artificial intelligence, aka chat gpt 4 is actually correct. What tools can I use to verify findings? Chop chop is incomplete and only provides target sequences etc.
@adwoabiotech10 ай бұрын
That is way out of my league but best of luck in your search. Feel free to share your findings if you feel so inclined :)
@magnificentwoman10 ай бұрын
Amazing!
@punintended475410 ай бұрын
Informative video. I would like to ask a question about detection limit for ion selective electrodes (ISE). Have you worked with ISE before?
@adwoabiotech10 ай бұрын
Thanks. I'm sorry that I am not familiar with detection limits for ISE.
@mariecenoble888410 ай бұрын
Hi. My mom was recently diagnosed with this virus (HTLV-1) and now has complete paralysis of her lower extremities. Is there a group that specializes in this advancement of the virus?
@adwoabiotech10 ай бұрын
I'm so sorry to hear! I am not aware of a group that specialises in advanced care of the virus but I'm linking the HTLV AWARE site in case you want to contact the physicians mentioned on the page for specific advice: I pray your mom receives the right care and support. www.htlvaware.com
@adwoabiotech11 ай бұрын
A poem by PHILLIS WHEATLEY upon seeing a young African Painter's (S.M.) work: To show the lab’ring bosom’s deep intent, And thought in living characters to paint, When first thy pencil did those beauties give, And breathing figures learnt from thee to live, How did those prospects give my soul delight, A new creation rushing on my sight? Still, wond’rous youth! each noble path pursue, On deathless glories fix thine ardent view: Still may the painter’s and the poet’s fire To aid thy pencil, and thy verse conspire! And may the charms of each seraphic theme Conduct thy footsteps to immortal fame! High to the blissful wonders of the skies Elate thy soul, and raise thy wishful eyes. Thrice happy, when exalted to survey That splendid city, crown’d with endless day, Whose twice six gates on radiant hinges ring: Celestial Salem blooms in endless spring. Calm and serene thy moments glide along, And may the muse inspire each future song! Still, with the sweets of contemplation bless’d, May peace with balmy wings your soul invest! But when these shades of time are chas’d away, And darkness ends in everlasting day, On what seraphic pinions shall we move, And view the landscapes in the realms above? There shall thy tongue in heav’nly murmurs flow, And there my muse with heav’nly transport glow: No more to tell of Damon’s tender sighs, Or rising radiance of Aurora’s eyes, For nobler themes demand a nobler strain, And purer language on th’ ethereal plain. Cease, gentle muse! the solemn gloom of night Now seals the fair creation from my sight.
@maryansong145711 ай бұрын
Wonderfully and artfully reviewed to reveal GOD'S Love in Phyllis"s heart . ❤
@adwoabiotech11 ай бұрын
"The world is a severe school master, for its frowns are less dangerous than its smiles and flatteries" - Phillis Wheatley The idea that African people were intellectually inferior was a justification for slavery. The legacy of that malicious and terrorising publicity, still lingers.