Hi! Not sure if this channel active. I use this apparantus. To help stabilise your liposomes, is it wise to have them extruded with buffer + sucrose/sugar cryoprotectant. Or are these protectants added after extrusion (prior to freeze drying the lipids)? I find that it is very tough to extrude manually when u add these sugars, it makes the buffer containing liposomes u want to extrude, more viscous. Harder to "push".

So detailed🎉🎉🎉Thank you very much!

Can we use ethanol to dissolve lipid mixture?

Thank you for this clear explanation! ❤

Which microphone you used for this recording, sounds very clean?

this is a great intro and crash course into lipidomics!

Thank you for the nice demonstration. Is it useful for the PLGA polymer extrusion?

Can i use acetone to wash?

Oh wow, this is almost as uninteresting as trying to figure out what makes people stupid enough to believe in scams like this...

Quack science for people foolish enough to believe what they watch on youtube.

For the frequency histogram slide, the referenced Quehenberger paper reports quantitative levels of over 500 lipids not 2500 lipids. The paper's supplementary tables contain a total of about 588 listed lipids which matches the total in Table 1 of the paper. For the min and max Log10 pmol/mL for the supplementary tables is -2 and 6.6. Is the histogram slide correct?

How I can get it ?

Nice, thank you for this video! I wish more companies would make these. Well done

Thanks for the video

Awesome

can the filter supports be reused?

catalog number?

Thank you very much. I'm ready to ace the paper now

its really helpful for starters

Thank you for the demonstration! It was very clear and helpful :)