nice explanation, very helpful, but what if the unknown matrix signal is negative, suppose -0.345 AU?
@Homme95 ай бұрын
It's really hard to believe that such great teachers exist in this broken education system. Ma'am, you are just awesome! Thank you for making these videos.
@robyngoacher85215 ай бұрын
Thank you - I appreciate the praise. Sadly, the broken education system drove me out of it and I work in industry now. But I did enjoy the teaching part of teaching!
@Homme94 ай бұрын
@@robyngoacher8521 You hang in there, Ma’am!
@nassimalijani82695 ай бұрын
This was perfect. Thank you so much.
@ThangPVan_5 ай бұрын
Hi, can I replace the concentration with the weight fraction?
@fatimabatool65306 ай бұрын
Book reference please
@juancocu6 ай бұрын
Amazing explanation, thank you very much
@roritadrawingtuto56276 ай бұрын
Thank you so much
@niloofarkh47796 ай бұрын
thank u so much
@user-yb4dz7pl2h7 ай бұрын
Can you add in Kw with the equation? Do I just need to add a 1/[H+]kw or something like in the charlot equation
@finno-px6of8 ай бұрын
Amazing Video!!!
@Baesjegutten8 ай бұрын
Thank you so much! The formulae always scare me, but this was a lot easier to understand than my lectures. I finally get it! (My exam is tomorrow lol)
@leiladerakhshan988110 ай бұрын
You are the best teacher😊
@woworo31510 ай бұрын
Thank you so much for very helpful video! I have one question. If I got 90% recovery for my protocol. Then, I got 10 ug/kg for my sample. Do I have to calculate back to 100% because of my protocol can detect only 90%.
@laithanmar9111 ай бұрын
You are genius
@pravingaonkar6292 Жыл бұрын
I think F value is wrong 😅 it's 1.25
@khalidhakimi4839 Жыл бұрын
Good
@ananyamohanty7088 Жыл бұрын
Thank you for the great video. While we are taking the dilution into account, won't we also consider the reduction in sample volume caused due to signal measurement? The V0 becomes a variable then..?
@robyngoacher8521 Жыл бұрын
Your question relates to whether "variable volume" or "constant volume" standard additions are done. In variable volume standard additions, the exact same solution is measured, spiked with standard, measured again, spiked with more standard and measured again (and so on). In that kind of standard addition, you cannot use a measurement technique that consumes sample. If you will consume the sample during measurement (even if that means losses due to transferring into and out of a cuvette for spectroscopy), then you should use constant volume standard additions. In constant volume standard additions, the V0 is the same for all flasks and you are able to do the math without needing to account for change in V0. So, no, the V0 does not become a variable. I hope that helps.
@serenesoulbd3487 Жыл бұрын
Nice
@terrycarver1255 Жыл бұрын
Wonderful presentation...very informative. Thank you
@styloxzvampire1283 Жыл бұрын
Is this is how we calculate GC yield?
@healthinsightsrothchildlll4402 Жыл бұрын
The volume is to low !!. Re do the Video. Thank you.
@engineeraqiii8649 Жыл бұрын
I think this formula you wrote incorrect. K= concentration/ Absorbance
@Shahidhsaraf Жыл бұрын
great work mam thank you so much
@lineakristensen1821 Жыл бұрын
Which text book are you using? I need to calculate an overlap integral for my BSc project. Thanks in advance.
@robyngoacher8521 Жыл бұрын
The book was Quantitative Chemical Analysis by Harris. Best of luck to you!
@lineakristensen1821 Жыл бұрын
@@robyngoacher8521 thank you. I really enjoyed your video 😊
@hazeldorothycesar5307 Жыл бұрын
thank you
@lramossanchez2 жыл бұрын
Great, prof! New subs here
@hanstsahnang45332 жыл бұрын
Thanks for this awesome video. Are blank solutions also known as control solutions?
@robyngoacher85212 жыл бұрын
The wording isn't always clear when something is called a control. In biological and medical experiments in particular, the control is something that doesn't have the affect-so no treatment or no drug. In that way, the control is kind of like a blank. But in analytical chemistry, the blank does not contain the analyte while control solutions contain the analyte in a known quantity. Therefore the blank provides information about the background and the control is used for checking if your method produces the right concentration of analyte. I hope that makes sense and answers your question.
@hanstsahnang45332 жыл бұрын
@@robyngoacher8521 thanks mom. Is very clear now
@haydenrogers54402 жыл бұрын
Is there a difference between matrix effect and recovery percent, or are they one in the same?
@robyngoacher85212 жыл бұрын
Matrix effect is the concept that analytes will respond differently in different systems. Spike recovery is the most common way to measure whether a matrix effect is present. If the spike recovery is close to 100%, then you can conclude that the analyte behaves the same in your sample (which is spiked) as it did in the set of simpler solutions used for calibration. I hope that helps.
@thecasualfront74322 жыл бұрын
just what i needed, thanks
@AnujSharma-hy1nn2 жыл бұрын
kzfaq.info/love/U1Tb8wdJDRJTV37Qqm3RLA
@contehspecial88242 жыл бұрын
In some cases the spike sample is about 50 to 100 times the known concentration. I analyze Cocoa samples I was at first having a problem with spike but following the 50 to 100 times I got my results
@gashayeyalew2 жыл бұрын
you.talk.so.fast
@mahditabatabaee58802 жыл бұрын
Many thanks
@ntandazo54042 жыл бұрын
❤️💯 the way you just saved me from studying this topic all over again from scratch 😃
@sattibabvanumu86122 жыл бұрын
would be able to help me clear my doubts that what factors decide the type of calibration either linear or logit calibration? and also what determines number of standards required for an assay calibration?
@justineninoinocencio61092 жыл бұрын
IS murexide Triprotic?
@esthermarie77212 жыл бұрын
Thank you so much
@minhazulislam91312 жыл бұрын
You saved me. Thank you so much.
@user-ek1dk7zc6g2 жыл бұрын
Oh, dear, this is what I urgently need to learn. Thank you very much
@user-ym6xc2ez5u2 жыл бұрын
You are so cute!!
@abdulsarwary87642 жыл бұрын
can you speak louder i cant hear you
@soundravallyrajendiran56942 жыл бұрын
Great lecture. thanks. can you please post the links of other 3 related videos here
@junaidrehmani98292 жыл бұрын
Hi Robyn would you refer to a source to help with determining pre validation parameters in a lab?
@FieldsWalker2 жыл бұрын
Thank you! What book can you recommend to read about general principles of chromatography?
@robyngoacher85212 жыл бұрын
Any edition of Harris's Quantitative Chemical Analysis provides a good basis.
@robyngoacher85212 жыл бұрын
chem.libretexts.org/Bookshelves/Analytical_Chemistry/Supplemental_Modules_(Analytical_Chemistry)/Analytical_Sciences_Digital_Library/JASDL/Courseware/Teaching_Instrumental_Analysis_without_a_Textbook is also a great free online resource
@SG730882 жыл бұрын
@@robyngoacher8521 Fantastic lecture. Please take lecture on Preparative HPLC and spectroscopy.
@ala75152 жыл бұрын
Thank you - very helpful!
@drelizabethomindeogaja23352 жыл бұрын
Wonderful video. Which textbook would you recommend for pharmacy undergraduate students