Hi. Thank you for your comment and question. Actually, Cholesterol content in the membrane is extremely important in regulating the fluidity of the plasma membrane, especially with temperature changes. At High Temperatures: Cholesterol tends to stabilize the membrane by making it less fluid. It restricts the movement of phospholipids, which can prevent the membrane from becoming too permeable or leaky. At Low Temperatures: Cholesterol helps to prevent the membrane from becoming too rigid by preventing the phospholipids from packing too closely together. This ensures that the membrane remains somewhat fluid, which is essential for proper cell function and for maintaining membrane proteins’ mobility and function.

Glad it was helpful!

The value of c can be positive or negative based on whether the intercept is made on the positive or negative side of the y-axis, respectively. You can force intercept to pass through "zero" to get a non-negative value.

​@@biologybasicsok

If your image has a scale bar through Zen Black then you should be able to calculate it's size without going through calibration. Instead, you can input that information in ImageJ and hit global to apply to all the images. You have to remember to change the calibration for each set of magnifications though and reset global accordingly. I would start by organizing the images into folders for each magnification e.g. all the 20x images together, all the 40x images together etc. Next, you would need to know what the scale bar provided by Zen Black is equal to in each case. I believe you should be able to find that information on the image bar itself. You would then open one of these images in Image J. Follow my calibration instructions and in this case measure the scale provided by ZenBlack and measure it in pixels, then set it to the measurement in uM shown in the image by ZenBlack. You can set it to Global and analyze ALL images with the same magnification taken by the same program. You would then repeat these steps for each set of images at different magnifications. To just measure intensity you do not need any calibration. Hope this helps.

@@biologybasicsThank you so much for this detailed answer. It helped so much. No amount of thanks is enough.

I am so sorry to hear what your family is going through. It is hard to watch someone you love go through this terrible disease. Unfortunately, our drug is not at the stage where we can use it for human trials yet. However, there are many new targeted therapies coming each week, one of which may help and/or provide her better quality of life. I hope the doctors you are working with can find other trials that she may be eligible for to give her that chance to be with you and her kids for a long time. You and your family will be in my thoughts and prayers.

You're most welcome

Glad you like them!

Yes. Ofcourse. The polarity is indeed from the electronegativity of oxygen. However, the mass/ size of the oxygen helps in keeping that electronegativity since it "hogs" the electrons longer in it's own valence shells.

Have you input the gene ontology results in reactome to see what comes up? Sometimes individual genes may not be directly related to a process but a combination of them together can indeed affect the pathway of interest indirectly.

@@MamaMimiNMe Thanks. How do you mean?

@@biologybasics it would be helpful mam.

thank you. I hope you decide to go back to finish up your studies.

yes you can. However, you need to make sure that both are saved either as a jpeg or tiff format.

Thank you mam.

😂

The topic is part of the same chapter as DNA replication. However, we did not cover it at that time and instead went over it separately

@@biologybasics Yeah! I think you wanted to cover some aspects of cancer (for example) as well, before you touched up on this! I really appreciate this! I also like the order in which you chose to cover the chapters in general!!

@@alexisfairo Yes. Thank you. I am glad you found it useful.

Thank you. Glad it helped

There should be a pdf link for all the slides available in the playlist description

drive.google.com/drive/folders/1K-q8eEb1dD6LWhFnlEdFB21CBpgGDgsp?usp=sharing

Thank you.