Neil Cross

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@psirer..9625 6 күн бұрын

Thank you for the content.

@MohammedAmeenah Ай бұрын

This is the best video have seen. Thank you so much

@user-uf1kq3mi3u Ай бұрын

Great vedio for cell culture indeed😊

@taylormarkel1094 2 ай бұрын

this is so useful thank you so much for making this!!!!!!!

@minato2363 2 ай бұрын

I guess the falcon tubes can be reused if autoclaved no need to incinerate them, I am saying this with respect to universities protocol no idea why its done in professional labs....

@kosheeka 27 күн бұрын

The best practice is to avoid any type of cross-contamination during the study. Cross-contamination can be expanded to include chemical contamination that may occur if autoclave is not properly carried out. This is a common practice in many laboratories.

@user-kc2uk2lv2h 2 ай бұрын

Does this really follow aseptic practice? I doubt that。

@anjalisingh-vq2jv 2 ай бұрын

can you plz tell the concentration of sodium alginate used and also mention the solvent for alginate solution?

@sadiahaquekhan6003 3 ай бұрын

Hi Neil, I am facing contamination continuously in my cultured cell. I checked my media and My insect cell media which i prepared manually using 2x sfc media, gentamycin, P/S ,serum and cell culture graded water. Could you please tell me how can I know that which of any cell culture reagent having contamination or not?? I sprayed alcohol vigorously on the reagent bottles, holders, pipettor, washed my hand, wearing gloves, rubbing my gloves with alcohol, keeping my empty plates and plastic container under the uv inside the hood, still contaminated media... :(

@canceronthebookss4975 Ай бұрын

You can autoclave the media if you think any component of the media is causing it

@AmruMagdy 3 ай бұрын

@AmMalak-hd9di 3 ай бұрын

Why he put 1.2 instead of 120 ? Someone explain please

@welelameka 3 ай бұрын

Thank you!

@JYOtiRaNJanMANgaRaj 3 ай бұрын

Thank u so much 🙏😊🙏🙏

@user-el5ur1tq1d 4 ай бұрын

extremely helpful

@user-ck4hp2eo2p 4 ай бұрын

Very good presentation

@demonwolf6195 4 ай бұрын

Wait why would you wash the cell to remove the medium with the PBS? I mean medium is required for cell growth, why remove it?

@kosheeka Ай бұрын

Washing with PBS removes unwanted components from cell cultures (dead cells, debris) and prepares them for further steps (staining, assays) without harming the cells. It provides a clean, consistent environment with the right salt balance for healthy cells.

@user-fg6ub4dd3t 5 ай бұрын

wonderful and well presented. hope i could an opportunity to work under your supervision and guidance. i am final year student of biotechnology.

@user-lp5qe1tv7d 5 ай бұрын

Very nicely explained sir thank you very much 👍

@thunderstorminmyblood3705 5 ай бұрын

Fantastic, thanks so much!

@maggieweber8419 6 ай бұрын

Is it "safe" to pour cells out of the flask? Especially when working with primary cell lines, would it be better to pipette and avoid the flask's lip? or does it not matter?

@kosheeka Ай бұрын

As you pointed out pouring cells out of the flask is generally not recommended. Pipetting is the preferred method for handling cells, especially primary cell lines which are more delicate. Pipetting allows for precision and reduced contamination risk.

@stooteebaruah9678 Ай бұрын

I had the same concern

@funny11744 6 ай бұрын

very informative and fast. very good technique! Could the counting be made without trypan ? It seems yes ,

@kosheeka Ай бұрын

While it's possible to count cells without Trypan Blue, this method doesn't assess cell viability. Trypan Blue helps differentiate between live and dead cells, ensuring you're working with a healthy population.

@mayamanek2182 6 ай бұрын

perfect explanation! simple to understand and the throughoughly produced visuals really helped

@matthewwagner47 7 ай бұрын

Is breathing on it adding potential contaminating?

@kosheeka Ай бұрын

Yes, breathing over the cell culture can be a source of contamination. You can introduce bacteria or fungi from your mouth and nose. In a sterile lab, wearing a head cover and surgical mask is essential to minimize this risk. Additionally, working in a biosafety cabinet provides further protection by filtering the air and creating a sterile work zone.

@vocalistguy9850 9 ай бұрын

Absolutely great

@WhatsInAName0 9 ай бұрын

Very helpful 👍

@businessgoose4355 10 ай бұрын

Thank you for sharing your knowledge! Great video

@kimmie2022 11 ай бұрын

Very thorough love this

@b-harmony Жыл бұрын

Thank you this was very helpful!

@aishahashmi2549 Жыл бұрын

Very informative 👍🏻👍🏻👍🏻

@marwatawfik3956 Жыл бұрын

thanks so much

@BlueSkyFight Жыл бұрын

No trypandblue?😮

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@petersamuelemeka8710 Жыл бұрын

Very informative; step by step explanation. This is the perfect video I craved for. Nice work, Cross

@oussboudjelthia4869 Жыл бұрын

Thank you soo much!!!! That was brilliant

@saminathapa829 Жыл бұрын

Very good video , thanks

@ChecedRodgers Жыл бұрын

Excellent and very thorough. A re-upload with louder audio would be fantastic.

@MES-S Жыл бұрын

An informative video

@danieloulhint7914 Жыл бұрын

Mr. Cross, thank you so much for this awsome video. It is really a review and informative for m.. Great presentation and easy to undersand. Kudos from Morocco.

@funny11744 6 ай бұрын

What happens with the phenol red ( from the composition of media): Is the phenol red able to change its color from basic to acidic and then after pH changes from basic to acidic ?

@kosheeka 2 ай бұрын

@@funny11744 Phenol red actually can change its color from basic to acidic and then back again! But under two conditions it will not be a suitable component: 1. Multiple cycles of going from acidic to basic can degrade the phenol red over time, making it less effective as an indicator. 2. If the media becomes very acidic (much lower pH), the phenol red might not change back completely even if the pH is adjusted upwards. This is because the cells themselves might be stressed or dying, causing a permanent shift in the media's pH.

@funny11744 2 ай бұрын

@@kosheeka thank you for information.

@brunadepaula5640 Жыл бұрын

It was very helpful!! Thanks!

@shahnawaza6710 Жыл бұрын

Thank you for this nice video

@deepalishenoy6705 Жыл бұрын

Very nicely explained sir. Thanks a lot..

@ShakespeareCafe Жыл бұрын

"Avoid pouring media and reagents directly from bottles or flasks." This is major violation of the sterile technique. Pouring media or PBS introduces aerosolization or spillage of liquids leading to cross contamination or contamination in general. Even though it may seem wasteful, serological pipettes are much cheaper than a ruined culture and loss of cell line.

@greencard1245 Жыл бұрын

I love your videos! thank you!

@estefanycamilabomfimdossan2308 2 жыл бұрын

This is an amazing work! Thank you so much!!

@funny11744 6 ай бұрын

Question : Regarding the stem cell culture in T 75 flask - using media without CO2 : Can we use a closed cap for T75 flask ?

2 жыл бұрын

Great video, do you have a protocol for MDA-MB-231 cells?

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@mrx4814 Жыл бұрын

wow can you share your work or publications?

@lisaoney1973 Жыл бұрын

@@mrx4814 Yes sure

@InquilineKea 2 жыл бұрын

"HDAC inhibitors enhanced the acetylation of the 20S proteasome, which correlated with an increase in the T-L activity of the proteasome in mouse and human myocardium "

@Gts2pro 2 жыл бұрын

Hi thanks great video , so we was taught to use aseptic technique by spraying anything going in the the fume hood ,

@kosheeka 25 күн бұрын

It is always a good practice to keep following it.

@valev2839 2 жыл бұрын

Thank you for this video, however I can't find the tutorial for counting cells :(

@emilytravis2417 2 жыл бұрын

How are the cells adhering to the flask enough so they're not dumped out with the fetal calf serum?

@valev2839 2 жыл бұрын

Because he is using anchorage-dependent cells, cells that only grow if they adhere to a surface. The cells are simply doing what they would do in vivo, inside the body. Cells that belong to tissues produce molecules that mediate both adhesion to the surface and cohesion with each other in order to actually form the tissue. On the other hand, red blood cells for example are usually in circulation in our blood so they wouldn't naturally adhere to a surface.

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