Could not really understand when he said mobile phase is weakened when we add water. If µ_water = 1.85 and µ_MeOH = 1.70, water is more polar than methanol. Wouldn't we be making mobile phase stronger by adding water, then??
@AliRaza-wp8tr Жыл бұрын
Because most of the time your analytes are non polar so by increasing water % means increasing polarity and anlyte will not love this composition hence now it attracts more towards stationary phase