I think that means the embryo died. Slowly guide the lower blade of the scissors into the egg being sure to keep the tips up against the inside of the shell so, you don't hurt the embryo.

@@Educationalcourses After I took out the embryo I thought it was about a kind of disease of the hen or because the food of the hen - the embryo seems to be full of blood. and it has no life- not any movement- but it has at least 50% of the size of a healthy embryo .

It is not necessary if the incubator is not closed hermetically (ventilation grid partially open!) and special Petri dishes with spacers between lid and dish are used.

I think this would help you: www.ncbi.nlm.nih.gov/pmc/articles/PMC10062638/

Here is it: cbsn.neuroinf.jp/database/item/id/ImageEP

We are truly grateful for your comment and value your feedback. If you would like to show your support, you have the option to purchase a super thanks.

They are called towel forceps or towel clamps.

@@Educationalcourses thank you so much 🙏

Isolation of crude mitochondrial fraction: 1) Grow 1 L of the wild-type strain BY4742 culture for 48 h. 2) Pour culture in 500-ml Nalgene centrifuge tubes. Balance the load and harvest cells by centrifugation for 5 min at 3000 x g at room temperature. 3) Decant supernatant and resuspend pelleted cells in 250 ml of dH2O. Pellet cells by centrifugation for 5 min at 3000 x g at room temperature. 4) Decant supernatant and resuspend pelleted cells in 250 ml of dH2O. Pellet cells by centrifugation for 5 min at 3000 x g at room temperature. 5) Determine wet weight of cell pellet. 6) Resuspend pelleted cells in DTT buffer [2 ml of buffer/g (wet weight) cells]. 7) Transfer the cell suspension to 50-ml Falcon plastic tubes. 8) Rotate the tubes at 70 rpm in a shaker for 20 min at 30°C. 9) Harvest cells by centrifugation for 5 min at 3000 x g at room temperature. 10) Resuspend pelleted cells in Zymolyase buffer without Zymolyase [7 ml of buffer/g (wet weight) cells]. 11) Pellet cells by centrifugation for 5 min at 3000 x g at room temperature. 12) Resuspend pelleted cells in Zymolyase buffer without Zymolyase [7 ml of buffer/g (wet weight) cells]. 13) Transfer cell suspension to a glass flask. 14) Add the powder of Zymolyase-100T [1 mg of Zymolyase-100T/ g (wet weight) cells] to the cell suspension. 15) Rotate the flask with cell suspension at 70 rpm in a shaker for 30 min at 30°C. 16) Transfer spheroplasts formed due to the digestion of the cell wall with Zymolyase-100T to 50-ml plastic centrifuge tubes. 17) Pellet spheroplasts by centrifugation for 8 min at 2200 x g at 4°C. **All subsequent steps should be carried out at 4°C or on ice. Suspensions of spheroplasts should be handled gently using pipettes with cut tips to avoid breaking organelles.** 18) Resuspend pelleted spheroplasts in ice-cold homogenization buffer [6.5 ml of buffer/g (wet weight) cells]. Pellet spheroplasts by centrifugation for 8 min at 2200 x g at 4°C. 19) Resuspend pelleted spheroplasts in ice-cold homogenization buffer [6.5 ml of buffer/g (wet weight) cells]. 20) Transfer cells to a pre-chilled on ice glass homogenizer. Using a tight pestle, homogenize the cells by making 15 strokes of the pestle. 21) Add 1 volume of ice-cold homogenization buffer. 22) Transfer homogenized spheroplasts to 50-ml plastic centrifuge tubes. 23) Pellet unbroken cells, nuclei, and large debris by centrifuging for 5 min at 1500 x g at 4°C. 24) Centrifuge the resulting supernatant for 5 min at 3000 x g at 4°C. 25) Centrifuge the resulting supernatant for 15 min at 12000 x g at 4°C. 26) Decant supernatant and resuspend pellet in ice-cold homogenization buffer [6.5 ml of buffer/g (wet weight) cells]. 27) Centrifuge for 5 min at 3000 x g at 4°C. Centrifuge the resulting supernatant for 15 min at 12000 x g at 4°C. 28) Decant supernatant and resuspend pellet in 3 ml of ice-cold SEM buffer.

Not all of them, some of them are from 2008 and the rest are more recent.

@@Educationalcourses thanks for the reply. the older ones from 2008 where do they originate from? I’m just curious :) were they posted on a different platform?

عوزه اتواصل معك عندى مرض جينى منذو ولدتى حتى الآن

1. We see some sort of vessels autolysis in some embryos(quite often). 2. Egg content becomes cloudy and turns into a paste(also often). We are suggesting contamination or lack of O2. Maybe you have faced such things and have some tips?

@@dmytrokrasnienkov1645 I suggest sealing the window so tight to prevent any air bubbles, also during the windowing process, many eggs developed cracks in the shell so carefully check up the egg after windowing.

Yes, there is a purification process. you can follow the instructions of the Epicentre MasterPure Yeast DNA Purification manual to know how to perform it.

I think, it is 2011 or 2010

Here is the pdf, www.ncbi.nlm.nih.gov/pmc/articles/PMC2565855/

Please reply..

Here is it: After data points have been entered, generate a line of best fit and corresponding slope equation.Find the change in absorbance in the test samples by calculating the difference between the test sample absorbance and the absorbance of the test blank. Inserting the absorbance value for one of the test samples into the slope equation and solving will result in the micromoles of tyrosine liberated during this particular proteolytic reaction. To get the activity of enzyme in units per/ml, perform the following calculation:(umole tyrosine equivalents released) x (11) Units/ml Enzyme = __________________________________________(1) x (10) x (2)11= Total volume (in milliliters) of assay10= Time of assay (in minutes) as per the Unit definition1= Volume of Enzyme (in milliliters) of enzyme used2= Volume (in milliliters) used in Colorimetric DeterminationTake the number of micromoles tyrosine equivalents released obtained from the slope equation and multiply it by the total volume of the assay in mls, which in this case is 11mls. Divide this value by three other quantities: the time of the assay, which here ran for 10 minutes, the volume of enzyme used in the assay, which was varied (let's use 1ml), the volume of milliliters used in colorimetric detection, which may differ based on your cuvette. Here it is 2 mls.

@@Educationalcourses I am asking your slope of standard curve? I get 0.008 slope of standard curve and once get 0.002 so which one is correct so I want to know your slope.

@@yatrimehta381 I think it is .8 but this is at 660nm

@@Educationalcourses means I get 0.008 standard slope which is not correct(I also take it at 660nm) ? I should get 0.8 right?

@@yatrimehta381 As I remember it could vary depending on the type of the sample. So, all you should concentrate on is that your sample readings aren't outside the linear range.

We add TCA to stop the reaction after that we filter each of the test solutions and the blank using a 0.45 um polyethersulfone syringe filter.

​@@Educationalcoursesbut we add tca in the standard curve which inhibits the formation of color so we not understand it.( if add TCA in Standard it's give yellow color and after 24hr show blue color so I think TCA effect on the FCR- tyrosine reaction)

@@yatrimehta381 I am really uncertain about the exact reason behind the outcome you are experiencing, but I can provide you with the protocol for setting up standard curves. 1)To each set of four vials, add 5mls of our 0.65% casein solution. Let them equilibrate in a water bath at 37°C for about 5 minutes. 2)Add varying volumes of enzyme solution that will be tested to three of the test sample vials, but not the blank. 3)Mix by swirling and incubate for 37°C for exactly ten minutes. The protease activity and consequential liberation of tyrosine during this incubation time is what will be measured and compared between test samples. 4)After this 10 minute incubation, add the 5 mls of the TCA reagent to each tube to stop the reaction. Then, add an appropriate volume of enzyme solution to each tube, even the blank, so that the final volume of enzyme solution in each tube is 1 ml. This is done to account for the absorbance value of the enzyme itself and to ensure that the final volume in each tube is equal. 5)Incubate the solutions at 37°C for 30 minutes.During this 30 minute incubation, you may want to set up your tyrosine standard dilutions. 6)Use 6 dram vials (dram vials can be substituted with polypropylene tubes) that can easily hold 8 mls. To the six vials, add the 1.1 mM tyrosine standard stock solutions with the following volumes in mls: 0.05, 0.10, 0.20, 0.40, 0.50. Don't add any tyrosine standard to the blank. Lower standards may be needed for impure test samples that will yield little color change. Once the tyrosine standard solution has been added, add an appropriate volume of purified water to each of the standards to bring the volume to 2 mls. 7)After the 30 minute incubation, filter each of the test solutions and the blank using a 0.45 um polyethersulfone syringe filter. Filtration is required to remove any insolubles from the samples. 8)Add the filtration 2 mls of the test samples and blank filtrate to 4 dram vials that can hold at least 8 mls. The same type of vial in which the standards were prepared can be used. 9)To all of the vials containing the standards and standard blank, add 5mls of sodium carbonate. For best results, add 1 ml of Folin's reagent immediately afterwards. Add sodium carbonate to regulate any pH drop created by the addition of the Folin's reagent. Add sodium carbonate to the test samples and test blank. These solutions become cloudy after the addition of sodium carbonate. 10)Add the Folin's reagent, which will react primarily with free tyrosine. Mix the dram vials by swirling and incubate at 37°C for 30 minutes. 11)After this incubation, you should notice that the standards have a gradation of color correlating with the amount of tyrosine added; the highest concentrations of tyrosine appearing darkest. You can also notice appreciable color change. 2mls of these solutions are filtered using a 0.45 um polyethersulfone syringe filter into suitable cuvettes. 12)Now that the assay is performed, you can proceed to the spectrophotometer to record our absorbance values.

Both are good except that in azocasein, it functions by releasing the azo-dye for absorbance detection. Casein operates by releasing aromatic amino acid residues which react with Folin's reagent to form chromophoric products

KCl should be pre-warmed to 37°C, First, add 3 ml, invert the tube to a horizontal position just to mix the cells with the hypotonic solution then add the remaining 2 ml and keep warm at 37°C for 15 minutes. Be careful, the time of incubation is important to acquire good chromosome spreads.