2.9 Separation of Photosynthetic Pigments by Chromatography (Practical 4)

No video

2.9 Separation of Photosynthetic Pigments by Chromatography (Practical 4)

Рет қаралды 294,839

Stephanie Castle

Күн бұрын

Пікірлер: 60

@rondayaram5792 3 жыл бұрын

Incredibly detailed. Well done. Thanks for sharing

@nguyenkienam2331 3 жыл бұрын

this helped me a lot in lab mixture separation report

@37KA4 3 жыл бұрын

Hi please can I contact you via email ..

@anantmalik17 Ай бұрын

Absolutely helpful and on the point. Thanks. My lab practical is on it's way. It helped a lot

@idkhahahaha Жыл бұрын

but what does the Rf value actually tell you about the pigment?

@breeze3430 2 жыл бұрын

Thank you! This is a helpful guide for our Plant physiology laboratory activity

@teacherjayzeelynvanessacor6889 Жыл бұрын

9 ⁹k

@drewhenderson8223 10 ай бұрын

Your welcome

@DeepikaSharma-io7bl 2 жыл бұрын

Well done mam it's really very helpful video for us keep create video for us

@Kwork1104 Жыл бұрын

Helpful video Must all bsc students watch this before exam❤❤

@deminigamage9438 2 жыл бұрын

Thank you so much! I didn't understand anything given in my lab manual. But this is very clear.

@phenyotshabang6394 3 жыл бұрын

Can someone please explain why acid washed sand and anhydrous MgSO4 are used in the preparation of the extract of this kind of chromatography.

@hotpepper7782 3 жыл бұрын

Well my bio teacher said the acid washed sand was just there to make the grinding process easier about the anhydrous MgSO4 idk y it's there.

@peneloppedjeakoua1570 9 ай бұрын

Very useful for my experiment tomorrow

@kennyyong7694 4 жыл бұрын

Excuse me, it is the same procedure for thin layer chromatography right?

@Palak..15 10 ай бұрын

Yup:)

@khushiparmar7444 6 ай бұрын

Solvent system which solution use?? Can you please tell me

@shristiasylum 3 жыл бұрын

Good job dear👍

@zmazn Жыл бұрын

what should I do or what does it mean if the carotene keeps ending up being the last one below? I tried it 2 times and its always the same and im afraid the school lab wont let me do it again and i have to make a lab report about it with my picture in it.. I used spinach leaves btw

@misskhan8328 2 жыл бұрын

your english is too good

@sabiha23467 Жыл бұрын

Thank you madam I understand very well

@sashaart781 2 жыл бұрын

Can I ask what solvent did she use? Please answer me 🙏

@TheHexbugfan 4 жыл бұрын

Ms. Webber sends her hellos!

@ayodelebello1145 4 жыл бұрын

How much acetone is used for each leaf sample?

@detss3795 4 жыл бұрын

not just acetone 5 ml solvent which contain 9 part Petroleum ether and 1 part acetone

@detss3795 4 жыл бұрын

@Elijah Tam-od Ok

@roshnimathew3209 2 жыл бұрын

Instead of ether which solvent can be used?

@climax7260 3 жыл бұрын

Thank you it helps

@niloypaul34 7 ай бұрын

thank you

@Idk-ij1yy 2 жыл бұрын

Is there any way to improve this method

@Leen-ih8ql 3 жыл бұрын

what are the methods or ways of separating plant pigments?? only TLC and paper chromatography ?? please I need help

@videoteaching47 2 жыл бұрын

u can do through chemical method as well.

@siddikpk8385 3 жыл бұрын

😊 🙏 thanks

@pawsitivebhumika6462 3 жыл бұрын

Thanku so so much❤

@afolabifakanbi7782 3 жыл бұрын

Tanks

@judeigho7254 3 жыл бұрын

Is the eluting solvent polar of non polar?

@evelynt.britos2863 4 жыл бұрын

What’s the significance and use of Rf value?

@evelynt.britos2863 4 жыл бұрын

After determining Rf, what’s next? Does it end in finding the Rf? Thanks.

@martajackson11 4 жыл бұрын

Hi, biology student here! From what I know, the Rf value is one of the methods you have to distinguish between the several photosynthetic pigments you can find on a leaf. You can also identify the pigments just by looking at their color. Basically if you have a Rf value close to 1, that means that the pigment moved quite a lot and it was abel to do that since it a polar pigment. If on the contrary you have a low Rf value, that means that the pigment didn't move that much and thus has non polar groups in its constitution. Please correct me if I am wrong

@xyrildanmanuel783 2 жыл бұрын

hi chemistry student here. the Rf value is one of the information we use to pinpoint what the pigment might potentially be. chemists around the world do this chromatography on initially known compounds and they share and confirm the Rf values they have obtained in a database (or multiple databases like the one presented at the end of the video) so that future analysts would have a reference when repeating the same chromatography on the same compound. think of it like a pseudo-fingerprint of a certain compound although i believe not all compounds have unique Rf values

@ahappycoder2925 2 жыл бұрын

RF value is used to identify the pigment

@jimsamson7934 Жыл бұрын

It allows you to get a measurement for 5G or 3G radiation frequencies. That way, you can gauge how thick your tinfoil needs to be in order to block those signals