Paper Chromatography Experiment

Рет қаралды 265,365

4 жыл бұрын

This is an experiment to separate the various pigments found in green plants, using a popular and simple technique called Paper Chromatography.
Dear viewer/subscriber, if my videos helped you a lot (maybe you aced your exams as a student, or you won the admiration and full attention of your students as a teacher, or you got your techniques right as a hobbyist), then its my humble request if you could take a moment to consider supporting me on anyone of the following platforms:
Patreon : / membership
KZfaq channel membership: / @thesingtangpascienceguy
Patreon members and channel members will get exclusive access to members-only perks and added benefits such as free PDF notes, Q&A doubt clearing chat sessions, Experiment video requests, Member shoutouts, Behind the scenes and more.
Or if a monthly payment is not convenient for you right now, you may instead consider supporting me with a one-time thankyou gesture by clicking on the SuperThanks Icon below my videos next to the like dislike buttons. Pay the one-time-only amount that you feel is worth the help and benefit you got from my video. Apart from Subscribing to my channel and sharing it with friends, colleagues and students, you valuable one-time or monthly financial support will really help sustain this channel and make it possible to come up with even more engaging and educational content. Creating experiment videos involve hundreds and thousands of dollars put into procuring the requisite chemicals, equipments and labware to make a single video. To say that i will be extremely grateful for your support would be an understatement.
My other KZfaq channel: / @59secsoffunscience71
Facebook: / thomas.tungnung
Instagram: / thomas_tungnung
X: / thomastktung
#BioTechniques #PlantPhysioLab #ThomasTKtungnung

Пікірлер: 93

@MimiKyle 3 жыл бұрын

This really helpful for me before start self study of the lesson.. Thanxs so much..

@Omni-Kriss 3 жыл бұрын

Clear and concise! Thanks for making this :)

@sandeepsandhu9563 4 жыл бұрын

I really appreciate your work 😊..ND need lots more videos like this

@hayaelizabeth7609 3 жыл бұрын

Thank you for this video! Is very helpfull! I have two questions, what plant did you use? I'm reading an article where the xanthophyll has a Rf of 0.16 (it seemed very polar to me) is it possible that the differences between your experiment and the experiment from the article (they used spinach) in the xantophyll are because there are many types of xanthophylls (like lutein and violaxanthin) in different plants? Or why did the xanthophyll in the spinach stood behind chlorophyll b and yours didn't?

@Shynyan_Khumukcham Жыл бұрын

Clear and cut explanation with a well defined experiment. Thank you very much.

@timothydestiny3865 Жыл бұрын

Thank you so much! Just by watching this video, I understood the principle of chromatography. Keep up the good work!

@nasavirtualgroup3483 4 жыл бұрын

So easy to understand. Thank you.

@The_micro_bro 4 жыл бұрын

This is great thank you!

@a.rlearning3507 3 жыл бұрын

Very helpful during this pandemic👍

@cinaria66 3 жыл бұрын

I listen to this everyday before to I go to bed! Very calming voice and gives me great long sleep similar to death. I have no idea what you’re actually saying tho ;) Can’t wait for my chem test tomorrow!!!!

@sirenix6376 3 жыл бұрын

Goodluck on your test!

@cinaria66 3 жыл бұрын

Aliia T thank you!

@mushroomjack6261 3 жыл бұрын

Hope you did good on your test lmao

@vaibhavsingh2733 Жыл бұрын

@rakeshkumarswain1980 3 жыл бұрын

An excellent video lessons for layman like me..thank you

@sirenix6376 3 жыл бұрын

Wow you are a great asmr artist! This was so relaxing and helped me fall asleep easily! Keep it up man👍

@cinaria66 3 жыл бұрын

Aliia T same!!!!

@TheSingtangpaScienceGuy 3 жыл бұрын

(in asmr voice) Happy to know that my videos have started to have more than one application; helping some in their bio while also taking the life out of others ;) (No pun-casm intended)

@rehmanullahkhan2326 3 жыл бұрын

Thnx for the wonderfull expaination👍👍👍👍

@theunionofarmforces2829 4 жыл бұрын

Thanks for showing best video

@btsaddict6816 5 ай бұрын

Great video!! Very helpful

@unnamed__3 2 жыл бұрын

Great job... Well explained 👍

@shohidulhasan6255 Жыл бұрын

This is very easy and helpful experiment for our practical exam..

@poornimaranawaka9403 3 жыл бұрын

Really helpful Thanks😊🙏

@mineberrakhalik1020 3 жыл бұрын

Thank you for this helpful video

@sanjidhasan1428 3 жыл бұрын

Thank you.Actually i was looking for this

@antonvathan1009 4 жыл бұрын

more helpfully thank u

@ifam4076 2 жыл бұрын

Amazing explanation 👏👏👏👏👏👏👏👏👏

@ajoymondal7173 3 жыл бұрын

Very nice explanation

@krupashah4561 4 жыл бұрын

Ty sir good video

@okaythen9571 3 жыл бұрын

wahhhh bete moj moj moj ji karda very helpful video for student sir very good sir

@bbx_risbox1270 3 жыл бұрын

Thanks a lot for helping me... 🙏

@Khondekar 3 жыл бұрын

Very useful 👍

@md.shiponali1139 3 жыл бұрын

Impressive video...

@indiegirl02 3 жыл бұрын

very helpful while i can not afford offline classes for practical during pandemic.

@sanimations1458 4 жыл бұрын

Hey, thanks so much for this video. I have a science fair project and I’m doing this but I’m not sure what I want to test and compare in my experiment. If you have any ideas please tell me. I would appreciate it a lot.

@TheSingtangpaScienceGuy 4 жыл бұрын

Hi, you can try leaves of different colours and shades. I've also seen some videos where they separate the pigments contained in ink. You can also separate amino acid components in a protein rich food source. Although they will need a visualizing agent to be sprayed on to them and then slightly heated to be able to see the amino acid spots on the paper. But for this u will need Ninhydrin (a potent carcinogen) so i highly doubt if thats the right thing for a sc fair projct. So u can work on Leaves and inks. Good luck 👍 i'm here if u need help.

@sanimations1458 4 жыл бұрын

Thank you so much man!!

@SONG.SPOTLIGHT 2 жыл бұрын

Good work 👏 ♥️

@sharifahsarahasyikinbintis8688 3 жыл бұрын

Hi can i kow what plant did u use ?

@Madhurathegardener 3 жыл бұрын

Very inf. And helpful tq

@SamriddhiAnand 3 жыл бұрын

At 5:54 I just had a question Can I say that the first pigment spot which has been mentioned as chlorophyll b does it contain only the pigment chlorophyll b or it is a mixture of all the other pigments as well and gradually only carotene is left

@TheSingtangpaScienceGuy 3 жыл бұрын

Each spot is a single phytopigment. From the first spot (chl b) the remaining pigments except chl b will migrate to the second spot (chl a spot). From second spot, all other pigments except chl a will migrate further up to the third spot and so on. This groups migration and the leaving behind of one pigment type at each "stop/station" will continue until all the pigments have been resolved into different spots on the paper.

@_rueuphoria_ 2 жыл бұрын

Thank you ☺️

@raiseyouremaan801 3 жыл бұрын

Thankyou so much

@arnoldschwarzen-word 3 жыл бұрын

Thx sir! It'll be really helpful for my practical exam tomorrow

@zagzagkima Жыл бұрын

thank you!

@vallamsettylakshmiprasanna1413 Жыл бұрын

Sir, Why should we remove mibrib? Are there any substances released from mibrib that affect the pigments?

@whiss2665 3 жыл бұрын

What type of leaves hv nice colours to show on the paper?

@TheSingtangpaScienceGuy 3 жыл бұрын

You can perhaps use a mix of leaves of different colors; green, red, yellow, orange...... Or you can use a mix of different coloured inks. Never actually tried these "fun" experiments before though. Good luck 🙂 do lemme know the results. 👍🏾

@aviralpandey1977 3 жыл бұрын

Nice video sir

@niloypaul34 6 ай бұрын

thank you

@alien9321 3 жыл бұрын

Very good

@EQXGaming 3 жыл бұрын

helpful!

@nishjijiiiii242 2 жыл бұрын

what can be a substitute for the petroleum ether:acetone?

@TheSingtangpaScienceGuy 2 жыл бұрын

you may substitute it petroleum ether with something that is non-polar such as hexane.

@aqsaaqsarana7500 2 жыл бұрын

Why we only use petroleum ether: acetone combination instead of using any other solvent for chlorophyll

@alingheorghe556 4 жыл бұрын

What happened to the "My lab" video?

@TheSingtangpaScienceGuy 4 жыл бұрын

Had to pull it down for various reasons. Will come up with a better vdo in the foreseeable future 😁

@BushraBakhtawar Жыл бұрын

Thanks

@russellevangelista8283 2 жыл бұрын

Question, how are you able to determine the kind of pigment it shows??

@TheSingtangpaScienceGuy 2 жыл бұрын

standard textbook knowledge. This is a highschool level experiment performed as part of syllabi, based on experimental facts already investigated decades ago by pioneers in chromatography.

@russellevangelista8283 2 жыл бұрын

@@TheSingtangpaScienceGuy thank you very much

@sasikumarvaradharajan7651 5 ай бұрын

May i know, what type of leaf it is?

@TheSingtangpaScienceGuy 4 ай бұрын

i used amaranth leaf, as it was just nearby me. You can use any leaf as long as its green.

@itslmoanotlmao 2 жыл бұрын

hello sir how to know that our paper chromatography is fail?

@TheSingtangpaScienceGuy 3 ай бұрын

If u dont get the chlorophyll and carotenoid bands/spots. U need to recheck your solvent and use quality whatman paper.

@salmankhursheed2164 3 жыл бұрын

Wow it blown my mind sir

@francisalbin1729 2 жыл бұрын

Nice

@Ziaamontero1 Жыл бұрын

Where is the measurement by the way?

@traicyalcantara4766 2 жыл бұрын

so, where is the measurements?

@mikewazowskigaming3029 3 жыл бұрын

Wow

@patrinajohnpeterravindrana7669 3 ай бұрын

Whts the ratio of acetone petroleum ether mobile solvent??

@TheSingtangpaScienceGuy 3 ай бұрын

1:9

@napalacatherine1190 Жыл бұрын

I thank God how as given you wisdom

@vishesharrvansashitharan3593 3 жыл бұрын

hi there i did this experiment last week but the phaeophytin was invisible. why is that? is there any scientific reason for that ? thank you

@TheSingtangpaScienceGuy 3 жыл бұрын

What plant species, what solvent (or solvent combination), did u use?

@vishesharrvansashitharan3593 3 жыл бұрын

@@TheSingtangpaScienceGuy we used Sauropus sp as the plant species and an ether acetone solvent

@TheSingtangpaScienceGuy 3 жыл бұрын

@@vishesharrvansashitharan3593 its probably because the leaves from which you prepared the extract, are still young, green and actively photosynthesizing. Pheophytin, as you might have already known, is one of the breakdown intermediate products of chlorophyll, and this breakdown into pheophytin, takes place usually during leaf senescence. So, even if the fresh green leaf might contain a little bit of pheophytin, the quantity is not so much as to to be seen on a chromatogram. Ofcourse, if you do a spectrophotometric analysis , you will detect some amount. But If, for any reason, you still want to see pheophytin on the paper, you can acidify the leaf extract prior to loading on the paper, in that way the chlorophylls lose their Magnesium and become pheophytin. Or you can make a leaf extract from old, senescing leaves that have yellowed. You are sure to find a nice pheophytin band/spot from old leaves. This is all i know from my textbook knowledge. Correct me if i'm wrong. Goodluck.

@vishesharrvansashitharan3593 3 жыл бұрын

@@TheSingtangpaScienceGuy alright that was very helpful, thank youuu soo much , god bless you , this is the actual experiment for my practical test next week, again you've helped sooo much , thanks🙏