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00:00 introduction
01:40 tools used to edit the gene.
04:35 engineered nucleases
06:58 some definitions.
10:36 ZFN (Zinc Finger Nuclease)
16:44 TALENs (Transcription Activator Like Effector Nucleases)
20:44 CRISPR (Clustered Regulatory Interspaced Short Palindromic Repeat)
28:42 evolution of CRISPR
30:24 Tools used in CRISPR
31:27 Domains of CAS9 Nuclease
34:32 types of CAS9 Nuclease
36:36 native CAS9
38:02 CAS9D10A
41:11 Dimeric RNA guided FOKI Nucleases (RFN)
43:56 dCAS9
48:14 Repair pathways
49:47 CRISPR CAS9 applications
51:09 plant genome editing using agrobacterium transformation
#crispr #genomeediting #geneexpression
#scienceandtechnology #science #biology #biotechnology #genetics #moleculargenetics #biotechnology #genesis #genomeediting #genome