A Guide For Selection of Buffer for HPLC

Рет қаралды 20,191

2 жыл бұрын

When samples contain ionizable compounds, the mobile phase pH can be one of the most important variables in the control of retention in a reversed-phase HPLC (RP-HPLC) separation. However, if it is not controlled properly, pH can be a source of many problems. Since most compounds analyzed by RP-HPLC contain one or more acidic or basic functional groups, most mobile phases require pH control. For this reason, buffers are widely used. This video highlights some of the important aspects of mobile phase pH for the practical chromatographer

Пікірлер: 31

@annadevarasivaprasad3589 3 ай бұрын

I got a very well knowledge on Method development...tq u so much sir

@milind0808 4 ай бұрын

Good explanation sir will help in method development a lot while selecting a buffer.

@jaykumarbhatt8841 16 күн бұрын

Thank sir. You are genius

@rupanalam1369 2 жыл бұрын

Your videos are very helpful.

@ardurinocreater8529 2 жыл бұрын

Hi, Ample and crystal clear explanation. All the vedios are Bible of analytical chemistry. Thanks lot

@shrutidixit499 2 жыл бұрын

Lovely presentation sir... Thank you soo much

@rahulkulkarni3037 2 жыл бұрын

Really useful in method development process

@sohelinamdar437 2 жыл бұрын

Nice explanation Sir..👌 It's great learning from your video's

@suranjanpaul16 2 жыл бұрын

Dhanyawad *Gurudev*...

@jaykumarbhatt8841 Жыл бұрын

Very nice explanation 👌

@abdulmujahid4048 2 жыл бұрын

Thank you sir 🙏

@Jitturawat958 2 жыл бұрын

Thank you sir

@Learnwithsimon27 2 жыл бұрын

very nice

@SanjayThakur-rn4zs 2 жыл бұрын

Nice explaination sir , thank for that. My question is a molecule contain both basic and acidic functional group so retention of that molecule at acidic and basic pls explain

@dhwarkeshgupta8465 3 ай бұрын

Fantastic explanation sir Can I get the ppt which your we're used in this video.

@nabeelpt8444 2 жыл бұрын

Sir, How will decide weight of salt for preparation 1L buffer?

@MohammadSalim-sd5vb Жыл бұрын

Please let me know what are the reference you used?

@talsaniyaraman6423 2 жыл бұрын

Suggest book for method development on HPLC.

@veerucration Жыл бұрын

why we used in base pH in LC

@sairampolisetty331 3 ай бұрын

Hello sir please reply me for this doubt im having , how could you know pka value of particular buffer that is whats the pka of nah2po4 and what us exact pka value for na2hpo4 pls tell me sir

@bhavanipodili2358 2 жыл бұрын

Good explaination sir I have one doubt from 2nd question Why the acidic compounds are not ionized at lower pH? Please explain

@dils2678 Жыл бұрын

In acidic environment -COOH group dissociation will not takes place....thats y

@maheshwaghmare8346 Жыл бұрын

Please make video of how to predict polar and non polar molecule

@Vexins Жыл бұрын

Look up The Organic Chemistry Tutor on KZfaq. Learn to draw Lewis structures and learn how to draw dipoles is the gist of it though.

@subhanid6656 2 жыл бұрын

Hi sir, thank you for the explanation. My doubght is,when we choose kh2po4, K2hpo4, etc

@pharmagrowthhub3083 2 жыл бұрын

K2HPO4 (dibasic potassium phosphate) is used for alkaline pH and KH2PO4 (monobasic potassium phosphate) for acidic pH

@subhanid6656 2 жыл бұрын

What happens if I use K2HPO4 for acidic pH and vice versa ..what change can I observe.pls explain sir

@dils2678 2 жыл бұрын

@@subhanid6656 Again we need to adjust more the pH to nearer , Using common ion effect solution

@karnakarkadaru2317 2 жыл бұрын

Sir, i have a doubt, Buffer PH to be adjusted is based on our compound Pka value or buffer pka value

@pharmagrowthhub3083 2 жыл бұрын

First, decide the required pH based on your compound and retention requirement. Once you identify the pH requirement, the next step is how to achieve the pH. For that purpose, you need to think of suitable buffer salt with the closest pKa value