you are saving me. 5 minutes ago I was crying from not being able to understand my molecular cell biology textbook (first year med student), and now it all made sense. thank you. love your videos, please never stop
@pegboh33158 жыл бұрын
extremely clear and very easy to understand. Just to be clear @7:16 did he mean "if we place this cDNA in a prokaryotic cell"
@thachnamxanh7 жыл бұрын
yeah, I'm also a little confusing at that point. I think it should be "cDNA in a prokaryotic cell"
@greenzebra976 жыл бұрын
YEs he made a mistake
@ferengarajbrahma85326 жыл бұрын
Pannel Egboh lol, exactly, nonetheless his explanation is the best
@ferengarajbrahma85326 жыл бұрын
Best Explaination 💯/💯, chow chow !
@autumnleaves54195 жыл бұрын
Yes. By which he meant vectors.
@Anushka27049 жыл бұрын
Thank you so much for making such a complex topic so simple and easy to understand. I wish my textbook explained things the way you do.
@telatti5 жыл бұрын
i absolutely love your lectures! thank you so much for all the hard work you put in. you're saving our lives so we can become doctors and save many more lives!
@mahsaadib87793 жыл бұрын
You make my life 10 times easier!! thank you thank you thank you❤️❤️❤️❤️
@pershianemati66682 жыл бұрын
THANKOU SO MUCH SIR! only if my lecturers could explain as awesome as you... I love your content and videos it ALWAYS helped me through understanding fully . i hope wherever you are you be always healthy and create more awesome videos. God bless you.
@zainabbaqer47137 жыл бұрын
really perfect explanation. your are one of the best lecturers
@nyoomi7 жыл бұрын
Thank you so much! Could not have understood this without your help.
@promisendlovu85508 жыл бұрын
omg dis is very easy.....................l cant even believe this !!!! thank you very much Sir
@seenithambyjeevaranjan27359 жыл бұрын
Beautifully explained. Thanks very much for your great service.
@emmanuelolajide52818 жыл бұрын
Thank you... very easy to grab. Great teacher.
@amritasaha67384 жыл бұрын
nice class
@akshayas768 Жыл бұрын
Very awesome no words to say
@Navidmsv4 жыл бұрын
Very useful like other videos. Thanks.
@abdshremo43748 жыл бұрын
you are awesome man!!!!! what a great work
@vendolyncher50813 ай бұрын
thank you i love you
@thu-dungdoan99668 жыл бұрын
clear and easy to understand, thanks man
@sashathomas587 жыл бұрын
Out of curiosity I believe at 6.08 there maybe an error, unless I'm not thinking correctly. All eukar dna consists of polyA tails so therefore in order to prepare a cDNA library a poly-T primer is created for complementarity??
@imamsajid24635 жыл бұрын
eukaryotic DNA doesn't have a poly A tail, it is the mRNA that does, when the reverse transcription is happening the poly A tail is removed and DNA does not contain poly T tail, therefore you don't need a Poly T primer in order to prepare a cDNA library
@shoaib3496 жыл бұрын
Nicely explained. Please make a video on Mitronchondrial Biogenesis
@marjanm.hashemi57578 жыл бұрын
perfect!thank you
@farahqaryouti23147 жыл бұрын
wow 😍😍😍😍 super clear now!!
@busisiwenzima87973 жыл бұрын
great lecture
@ST-gd4eq8 жыл бұрын
Amazing video, but I think there may have been some errors. Please correct me if I am wrong. 1. On the bottom right, the cDNA is shown to be 5' to 3' and the mRNA is also shown to be 5' to 3', should the cDNA actually be 3' to 5' so that is is complementary to the mRNA? 2. In 8:35, you say that you can take the double stranded processed DNA and place it in the eukaryotic bacterial cell, did you mean prokaryotic or am I confusing the meaning of your sentence and that the eukaryotic refers to the DNA?
@zeromethanez8 жыл бұрын
yes i think youre right
@geremy20307 жыл бұрын
I also think you're right
@scorpianguitar7 жыл бұрын
For the first note, on the bottom right, the cDNA is shown to be 5' to 3' because it has been rotated 180 degrees. Notice the flat back bone is on top instead of the bottom of the bases, while the bases (perpendicular lines) are facing down. Imagine the cDNA was attached to the mRNA but then it was separated AND rotated 180 degrees to be put side by side so that they both are shown as 5' to 3'. I think that's what he did, but I am not sure.
@Sana-fp6yl5 жыл бұрын
yes actually the dna strand synthesised first must b 3'-5' nd cdna 5'-3' so thst it becomes complementary to the parent strand and has same polarity as the mrna strand...nd actually he meant prokaryotic bacteria
@ef49025 жыл бұрын
Thank you, you are awesome!!!
@Dr.Kurdi222 жыл бұрын
Thanks
@christinetj38264 жыл бұрын
wonderfully explained. I just have one problem with your lectures though. I have to have my inhaler next to me because you don't breath in between your explanations and that gives me short breath. It's not a joke I'm serious please take a breath sometimes. please!!
@karamalfakih94762 жыл бұрын
Is there some way to know each individual gene sequence So that we can use a specific restriction enzymes to put the gene in a vector?
@biswajit41348 жыл бұрын
thank you
@lenorasalae.69132 жыл бұрын
Thank you sir, vividly explained but i have a little worry. is there a possibility for the sscDNA to be re-transcribe into RNA? And given that DNA polymerase needs a free 3' OH to be able to form the complementary strand or betterstill it needs a primer for elongation to take place. what happens in this case? where does the sscDNA get its primer from?
@jonelgeneta95903 жыл бұрын
Assuming you have determined the sequence of a certain enzyme/protein product, how will you identify its correct DNA sequence (*some codons are redundant or wobbled) using the cDNA libary? just asking.
@malikbasharat71198 жыл бұрын
Very much helpful Sir have u posted lectures on plant physiology..I need them basically.
@stanlee53527 жыл бұрын
great! but how do u prevent from alternative splicing when producing pre-mRNA into mRNA as a template?
@JoelNHarris7 жыл бұрын
For stuff that relies on alternative splicing (ex. antibodies) I think you'd only be able to make cDNA for one transcript variant that codes for one specific protein
@sujitkundu65616 жыл бұрын
wow beautiful class
@arixii37967 жыл бұрын
do we need to place our double stranded cDNA inside a vector of a prokaryote? (ie plasmid/lambda phage) or can we just insert the cDNA molecule directly inside a prokaryote??
@whatsonmymind48487 жыл бұрын
Hey, really good video. One question. In another video about cDNA you said that to separate the DNA/RNA hybrid we have to increase the pH, because at a basic pH RNA gets hydrolysed but DNA not, so we will remain with the sDNA, here you say we heat them up to separate, which makes sense for me, but which of the two methods are right? Or can we use both? Has one more advantage?
@ishwaryaavvarissbn16062 жыл бұрын
Its easy to understand thank u.. Why it is called library !!?
@Sakshi-hf3pf8 жыл бұрын
that was very well explained, thank you. could you please explain subtractive hybridization as well? :)
@Ssmshakeeb8 жыл бұрын
If restriction enzymes cleaves by recognition of some specific sequence.. Why does it selectivly cleave genes differently? Isnt there a high chance of braking middle of genes?
@justinalmano82733 жыл бұрын
wonderful!!!
@mmaking86647 жыл бұрын
The mRNA strandis 5' to 3'. Shouldn't the complementary DNA strand be 5' to 3' also?
@TimetoWatch2477 жыл бұрын
No, because then it wouldn't generate the same mRNA transcript due the 5'-3' directionality of DNA polymerase. The cDNA generated is an antisense strand (non-coding) which binds to the sense strand, or coding DNA.
@4dham7 жыл бұрын
So if I place a DNA inside a prokarytic cell, the prokaryotic cell cannot synthesize the protein, because of the introns sequences the DNA contain?
@leokuo89957 жыл бұрын
yes
@sarfrazahmedfraz16113 жыл бұрын
Using word eukaryotic for prokaryotic
@lysannbock56085 жыл бұрын
Wow thanks man
@MarieBai5 жыл бұрын
Bravo!!!!
@sruthi.m198 жыл бұрын
very easy now!
@lisalasoya79792 жыл бұрын
An MNRA bacterial cells.
@santudas_957 жыл бұрын
thnx sir..
@soulofgeek26397 жыл бұрын
Please answer me ! I need help, why they are doing this cDNA, what are the domains of application (sorry for my english, il french )
@geremy20307 жыл бұрын
One application where cDNA is used is RNA-Seq: "While direct sequencing of RNA molecules is possible, most RNA-Seq experiments are carried out on instruments that sequence DNA molecules due to the technical maturity of commercial instruments designed for DNA-based sequencing. Therefore, cDNA library preparation from RNA is a required step for RNA-Seq." - RNA-Seq methods for transcriptome analysis. Hope that helps.
@soulofgeek26397 жыл бұрын
thanks
@rushabasnet46133 жыл бұрын
I think labelling of intron and exon needs to be reversed.