Building and Screening Genomic Libraries

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Building and Screening Genomic Libraries

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Күн бұрын

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Пікірлер: 41

@SirTrollingham 5 жыл бұрын

I appreciate the amount of effort you put into your lectures and illustrations.

@Sam-sw7sw Жыл бұрын

Oh my god thank you so much! I finally understand the entire process because of this video!

@alaamufawwez1602 9 жыл бұрын

Ur videos help me in my genomics exam.. Thanks aloooooot ⭐️⭐️⭐️⭐️⭐️

@alishbamirza4709 3 жыл бұрын

THANKS A BUNCH !

@myriampinkas7478 4 жыл бұрын

Does the restriction enzyme cut precisely one gene at a time or does the fragment sometimes include more (or less) than one gene? How does the restriction recognize where to cut?

@randomassguy 2 жыл бұрын

Fantastic video this greatly helped me understand this topic

@glilyjebamalardaviitkharag8763 5 жыл бұрын

Isn't it an audacious claim to make when you say that the restriction sites are present exactly at the intersection of 2 genes? I believe the restriction site locations will be extremely impossible to predict, as we never know where a palindromic sequence is supposed to be.

@fishyfishfishing_ 3 жыл бұрын

As far as I know, scientists use many different restriction enzymes on different copies of one genome to be sure to have the maximum representation (and to include the sequence they're seeking)

@adimchinakaonyejekwulum3293 4 жыл бұрын

Thank you. Your videos are super helpful.

@XoXHannah41XoX 8 жыл бұрын

You said that you cleave the larger DNA fragments into smaller gene fragments... but if the functions are not known, how do you known when you're cutting a gene itself out and not halfway though a gene? Do you look for start/ stop codons first or something? And how do you separate the genes into different beakers, is it based on differential centrifugation...? I'm a little confused!

@mayamaya-ry3eg 8 жыл бұрын

yes im confused as well. im assuming you can take the dna that has been run through the gel and then clone it and put them in beaker? i might be wrong, ive never run the gel electrophoresis before

@barrylitser3793 6 жыл бұрын

you have to carry out partial dna digestion to improve your chances of having atleast one intact copy of each gene because indeed restriction enzymes don't respect gene boundaries. Later on you can look if the gene of interest is present by using a complementary strand.

@ornitcohengindi90 3 жыл бұрын

you are so clear! i love your videos please make more

@Mr007amir007 8 күн бұрын

wow u r very good!

@zeinabebrahim7684 7 жыл бұрын

your video has helped me alot🌟🌟🌟🌟🌟

@lbarbaric11 6 жыл бұрын

Explained so well!

@gail4100 7 жыл бұрын

Thank you so much :) this was very helpful

@andredvs 6 жыл бұрын

your videos are awesome. thank you.

@nazninkhan9595 4 жыл бұрын

thank you sir......it's very heipful for me...…..thanks again

@soheebwains1507 6 жыл бұрын

this was awesome, thank you!!!

@hoangphucnguyenle76 4 жыл бұрын

many thanks! I fink it very useful and easy to comprehend

@kotikotordia7496 8 жыл бұрын

You're the coolest *_* thanks a bunch

@rezayekta2153 2 жыл бұрын

Thank you very much

@ArslanFathullah 5 жыл бұрын

Do we still (now) need a phage vector to replicate the gene fragments? Why can't we do a PCR to amplify the gene fragments in large number followed by insertion into e.coli and screen for our gene of interest?

@mahdiyehbigham6357 Жыл бұрын

great!

@rajwant_kaur 4 жыл бұрын

How many recombinants has to be screened with 90 percent probbality of getting a gene if the size of genome is 8000kb and library was constructed in puc vector Please answer

@sabaali7048 3 жыл бұрын

0.15

@sabaali7048 3 жыл бұрын

Use Clark and Carbon formula N=ln(1-95%)/ln(1-1/3)

@jyothisajjanapu2007 7 жыл бұрын

can you tell me how the probe is going to get hybridised with the dna of interest?and how we are going to seperate that hybridised dna for further process?