Amino acid charge at different pH and amino acid titration calculation problems

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Amino acid charge at different pH and amino acid titration calculation problems

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Amino acid charge at different pH and amino acid titration calculation problems - This lecture explains about the amino acid titration curve and this will teach you the process of calculating amino acid charge at different pH along with pka values and it will also help you to know the net charge of polypeptide chain from the pka values of amino acids.
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Thank you for watching the video lecture on amino acid titration curve and net charge problems.
This will also teach you about the amino acid charge pH and Pka values and the way to calculate net charge of amino acid.

Пікірлер: 327

@nahomelion 4 жыл бұрын

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@Yakshiii 2 жыл бұрын

this man is a king, explained so concisely what my professor could not explain in two hours... thank you sir!

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@nephronum 5 жыл бұрын

Can you explain why did you put carboxyl group in ionized state, whereas amino group was not ionized.(in cell conditions both are ionized)

@pntdnails8835 2 жыл бұрын

just got out of COVID quarantine and my prof didn't explain this at all and the figures were making it really confusing. I skipped ahead to the part I was confused and it took literally a minute and I completely understood this topic. thank you so much for this video that you created like 5 years ago

@YourManasi 4 жыл бұрын

Thanks a lot Sir,,,,you made it crystal clear....The concept about that become as clean as fresh water.... 🙏🙏🙏Keep going sir....

@shomusbiologyofficial 4 жыл бұрын

You're welcome. Glad to hear that you're getting benefit from my lectures

@Ceeceelu_ 3 жыл бұрын

Thankyou for simplifying this to me I definitely feel more confident for my exam.

@shomusbiologyofficial 3 жыл бұрын

You're welcome

@akankshabhatt3357 3 жыл бұрын

I had a hard time trying to understand this simple concept and finally it all made sense. Bless your soul for making such content, you are helping so many people including me to understand basic fundamentals in biochem/biology/chem.

@siddharthsingh5169 3 жыл бұрын

Shomu Sir , i like your personality and also the way of talking. wow

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@mdbadsha418 2 жыл бұрын

You are the best! I never understood this topic so clearly before. Same for many other topics you taught in your other videos. Thank you so much!

@shomusbiologyofficial 2 жыл бұрын

Thank you so much for appreciating my efforts

@thisariupamalika9924 5 жыл бұрын

Thank you, Sir. Your videos are really helpful for me to understand the concepts of biochemistry. And I'd strongly recommend to anyone to watch your videos to clear their doubts.

@shomusbiologyofficial 5 жыл бұрын

Glad to hear that you are getting benefit from the videos

@vishnuparihar5445 4 жыл бұрын

Thisari Upamalika ok

@dailydevosinc.4345 3 жыл бұрын

you've enlightened my understanding better. Thank you very much SIr

@shomusbiologyofficial 3 жыл бұрын

You're welcome

@kavitagarg4656 3 жыл бұрын

Too good explaination and easy to understand

@shomusbiologyofficial 3 жыл бұрын

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@sanjaisrao484 2 жыл бұрын

Excellent explanation

@shomusbiologyofficial 2 жыл бұрын

Thank you

@sanjaisrao484 2 жыл бұрын

@@shomusbiologyofficial welcome

@Empress426 2 жыл бұрын

Thank you for giving me some clarity on this topic, I am currently taking a self-taught biochemistry class with a very confusing professor. Thank you.

@shomusbiologyofficial 2 жыл бұрын

You're welcome

@makayla1774 Жыл бұрын

You made this so simple, thank you.

@shomusbiologyofficial Жыл бұрын

You're welcome

@she_is_a_hungry_raccoon 6 жыл бұрын

let me buy a beer for that man

@shomusbiologyofficial 6 жыл бұрын

Let it be

@andreusdagoat9958 4 жыл бұрын

Wow KZfaq has the best teachers!

@shomusbiologyofficial 4 жыл бұрын

Thank you

@1rrrrrrrrrrr544f Жыл бұрын

Eventhough there was a mistake,but thanks because you made me sure about my idea! I concluded the same thing when I studied amino acids alone!

@raviphougat2474 2 жыл бұрын

Excellent sir God bless you

@shomusbiologyofficial 2 жыл бұрын

Thank you

@theimprovist4158 2 жыл бұрын

You're the best💯

@shomusbiologyofficial 2 жыл бұрын

Thank you so much for appreciating my efforts

@gargisain5091 3 жыл бұрын

Your explanation is too much good sir ♥

@shomusbiologyofficial 3 жыл бұрын

Thank you so much for appreciating my efforts

@wycliffenyandika9017 2 жыл бұрын

Damn! You have no idea how long have been going through videos on KZfaq and still couldn't answer a question on this topic ,I'm preparing for MCAT, this will go a long way. Only way to pay you is to share the video

@shomusbiologyofficial 2 жыл бұрын

Thank you so much for appreciating my efforts

@rachna4140 6 жыл бұрын

sir can u make video on longpolypeptide chain like this. plzz soon becoz it gives idea how can calculate

@ranarajeshwariba1120 6 жыл бұрын

Sir me apke video se hi hamara pura syllabus ache se samaj me aa jata he so tnx and vert good work and very helpful for our study

@ankitaarvindbhai1059 4 жыл бұрын

thank you very much sir you're all vidio help me lot

@shomusbiologyofficial 4 жыл бұрын

You're welcome

@fizaali6039 Жыл бұрын

bro ur the best

@shomusbiologyofficial Жыл бұрын

Thank you

@bishwabhusankundu2385 5 жыл бұрын

Are you sure that the pI for glycine is 7 and not 5.97...🤔🤔 Isn't pI supposed to be calculated as (pKa1+pKa2)/2 For glycine it would be (2.34+9.60)/2=5.97

@Rohit.Kashab 3 жыл бұрын

exactly as we will get zwitterion in between these two ph's so he is wrong on this part

@israthossain6012 4 жыл бұрын

For Glycine when pH is 12 how does the net charge is -1? In N-H because of the deprotonation the charge is -1 and in COO- bond there is -1 charge so the total net charge is supposed to be -2. When pH=7 you are counting the charge of COO- is -1 to get the total net charge. Then why when pH=12 you are not counting the charge of COO- group -1 charge to get the total net charge? Can you please explain it?

@misbashabir2063 Жыл бұрын

Thank you sooo much sir .....

@shomusbiologyofficial Жыл бұрын

You're welcome

@minaafrasiabi4171 11 ай бұрын

Thank you!

@shomusbiologyofficial 11 ай бұрын

You're welcome

@janvimyadventures1310 4 жыл бұрын

Sir I have some confusion with glycine formula. In the video it is NH - CH2- COO-. Is it correct.

@soumyajitdas6686 3 жыл бұрын

Yrs

@pallabisarma8463 6 жыл бұрын

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@lubnasa3453 2 жыл бұрын

thank you sooooo much man

@shomusbiologyofficial 2 жыл бұрын

You're welcome

@ujalaabbas1861 5 жыл бұрын

Thanks for the video.. cleared the concept ❤️❤️👍👍

@hasankuliyev4211 6 жыл бұрын

is net charge would be -2 at pH 12 since there is already -1 (pH 12 does not change -1 and -1 stays -1) in carbonyl group and very basic condition(pH 12) rips out H from NH which makes -1 as you mentioned, So -1 + -1 =-2 isn`t it?

@mytwinze942 4 жыл бұрын

I agree with you ,we need clear this point

@lynnyuness7160 11 ай бұрын

You saved meeeeeeee thank youuuuu watching this video will help a lot of embaressed biochem students because of their stupid professor

@shomusbiologyofficial 11 ай бұрын

Glad to hear that you're getting benefit from my lectures

@dikshajha8279 4 жыл бұрын

Thankyou sir for making the concept easy to understand 🙏

@shomusbiologyofficial 4 жыл бұрын

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@somnathchanda1250 Жыл бұрын

Oh! what an explanation

@shomusbiologyofficial Жыл бұрын

Thank you

@meenublossom6860 6 жыл бұрын

Awesome lacture... 👌👌Thank you so much for clearing my concepts with amino acid charge calculation 🙏🙏😊

@shomusbiologyofficial 6 жыл бұрын

You're welcome

@pierrejeanes 5 жыл бұрын

@@shomusbiologyofficial multipel ?

@pierrejeanes 5 жыл бұрын

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@Rajkumar25679 Жыл бұрын

Tqsm sir ❤️

@shomusbiologyofficial Жыл бұрын

You're welcome

@Anjalish66 6 жыл бұрын

You are doing great job sir.. God bless you

@shomusbiologyofficial 6 жыл бұрын

+Anjali Shakya thank you. Glad you liked my lectures

@CrestScienceAcademy 6 жыл бұрын

Anjali km yes

@CrestScienceAcademy 6 жыл бұрын

@prasadmadane9711 3 жыл бұрын

Great sir🤗

@shomusbiologyofficial 3 жыл бұрын

Thank you

@taitopotato 2 жыл бұрын

Legend! Thank you so much.

@shomusbiologyofficial 2 жыл бұрын

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@shyambhavi10 3 жыл бұрын

Thank you sir 👍👍....

@shomusbiologyofficial 3 жыл бұрын

You're welcome.

@sjc5411 3 жыл бұрын

He taught the subject better than my professor

@shomusbiologyofficial 3 жыл бұрын

Thank you so much for appreciating my efforts

@mashab6374 3 жыл бұрын

Thank you so much!! It's so clear to me now!!

@shomusbiologyofficial 3 жыл бұрын

You're welcome

@skp-official 6 жыл бұрын

Your videos helped me lot in class 12

@kaitlynridgeway1405 2 жыл бұрын

god bless your soul

@shomusbiologyofficial 2 жыл бұрын

Thank you

@omethheenkenda2793 Жыл бұрын

thank you

@shomusbiologyofficial Жыл бұрын

You're welcome

@pixiepout 4 жыл бұрын

Thank you very much for this video it really helped with my studying!!

@shomusbiologyofficial 4 жыл бұрын

You're welcome. Glad to hear that you're getting benefit from my lectures

@nitinchauhan9075 6 жыл бұрын

Your videos are really helpful...I m totally dependent on ur videos. Thanks a lot...you are doing a great work

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+Nitin Chauhan thank you. Glad you liked my lectures. Please subscribe and share

@nitinchauhan9075 6 жыл бұрын

Shomu's Biology : No concept is difficult with your way of teaching.

@somyaagarwal627 8 күн бұрын

thanks

@shomusbiologyofficial 8 күн бұрын

You're welcome

@Kattiling 2 жыл бұрын

I don't get why the net charge of pH 12 is -1. Can someone explain why? I thought it would be -2, this is because there would be a negative charge from both the nitrogen and oxygen so (-1+-1=2). I don't understand why he ignored the charge in the oxygen in pH 12, but accounted the oxygen's charge in pH of 7.

@rudraseth5419 6 жыл бұрын

Sir could you plz elaborate how the molecule are protonated and deprotonated .

@abdulharrison4380 2 жыл бұрын

Thank you soooooooo much Sir!

@shomusbiologyofficial 2 жыл бұрын

You're welcome

@enashaswa613 3 жыл бұрын

where can i find part 2? it's not shown to me ,if someone could put its link i'd be thakfull

@surajbhowmik4404 3 жыл бұрын

sir how to find the next class?

@rajwinderdubb3222 3 жыл бұрын

Omg Thank you so much sir! you explained it very well

@shomusbiologyofficial 3 жыл бұрын

You're welcome. Glad to hear that you're getting benefit from my lectures

@somnathchanda1250 Жыл бұрын

will de-protonation of amine happen at pH 10 for developing a negative charge over -NH gr.

@highrepublic3264 3 жыл бұрын

thank you for this video friend

@shomusbiologyofficial 3 жыл бұрын

You're welcome

@farooha.s8411 2 жыл бұрын

Thanks you so much!! Where can I see the second part of the class? I need to know how to calculate the net charge for a polypeptide

@philasandexulu3304 4 жыл бұрын

Thank you 🤩

@shomusbiologyofficial 4 жыл бұрын

You're welcome

@YashSharma-ce1hk 2 жыл бұрын

Sir, I'm a JEE aspirant and I recently faced a problem on Biomolecules The question had three phases of glycine where [A]- Both acid part and -NH part was protonated [B]- Only the -NH part was protonated [C]- Both the parts was deprotonated pKa1=2.3 pKa2=9.7 The question asked if at pH=2.3, half of [A] is converted to [B] or not? So sir, can I consider it to be correct that yes it is converted, on the pretext that half part is protonated and half is deprotonated? (I am having confusion in the part where it asked 'converted', and I am not sure that half protonation would result in half conversion of phase [A] to phase [B])

@20tejas 6 жыл бұрын

please make a detailed video on thermodynamics of protein stability and vant hoffs equation

@shomusbiologyofficial 6 жыл бұрын

+20tejas okay. Will try to do that

@20tejas 6 жыл бұрын

Shomu's Biology thank you.☺

@srikantadash4603 4 жыл бұрын

Plse sir

@alejandrarodriguezcruz227 3 жыл бұрын

When KZfaq teaches you more than a top University :/

@kavyap4453 3 жыл бұрын

Thank you so much for this very simple and informative lecture

@shomusbiologyofficial 3 жыл бұрын

Thank you. Glad you liked my lectures

@dayangasyiqin2398 5 жыл бұрын

great job!

@noone-kd2jd 3 жыл бұрын

Thank you sir

@shomusbiologyofficial 3 жыл бұрын

You're welcome

@giftnamwanja7715 5 жыл бұрын

awesome thanks. God bless

@shomusbiologyofficial 5 жыл бұрын

You're welcome

@juhishrivastava8725 5 жыл бұрын

Very informative sir.

@shomusbiologyofficial 5 жыл бұрын

Thank you for appreciating my efforts

@mahaboobbashashaik5219 3 жыл бұрын

nice clarity

@shomusbiologyofficial 3 жыл бұрын

Thank you

@suvosreemukherjee6868 6 жыл бұрын

sir why only pH 7...It could also have been any value above2.3 and below 9.6..in that case also the charge would be zero i guess..??

@tirathjangra257 6 жыл бұрын

How to calculate the charges on acidic Amino acid and basic Amino acid at different ph

@shonalikrishnani8775 6 жыл бұрын

sir please solve the analytical part of csir net 2017.. your videos help a lot.. thank you so much sir.

@shomusbiologyofficial 6 жыл бұрын

+Shonali Krishnani I will

@rabianoor1589 3 жыл бұрын

Thank you so much sir i completely understand this topic🥰😅

@shomusbiologyofficial 3 жыл бұрын

You're welcome. Glad to hear that you're getting benefit from my lectures

@hamidazizi7658 3 жыл бұрын

thanks man, Greetings from Germany :D

@shomusbiologyofficial 3 жыл бұрын

You're welcome. Glad to hear that you're getting benefit from my lectures. Please subscribe and share

@philomax5657 6 жыл бұрын

thnks pro

@shomusbiologyofficial 6 жыл бұрын

+Biologie facile you're welcome

@sharmisthasharma3587 2 жыл бұрын

Sir pls make a video on how to calculate the absorbance of an amino acid ..

@shomusbiologyofficial 2 жыл бұрын

Okay

@riusgallery3397 4 жыл бұрын

Thank you sir🙏

@shomusbiologyofficial 4 жыл бұрын

You're welcome

@054gomathip7 3 жыл бұрын

When I get confused in some topic in biology ,I usually watch your videos, it gives clarity.tq sir

@kalpanadas8693 6 жыл бұрын

Sir can't understnd when pH=pKa..otherwise wonderful video

@adnanfaisal1085 2 жыл бұрын

i have a question regarding pH 12. Amine already has no H+, so why did u say deprotonation and write -1 on top?

@sudiptadhara1983 6 жыл бұрын

very informative

@shomusbiologyofficial 6 жыл бұрын

Thank you

@sudiptadhara1983 6 жыл бұрын

sir I am a student of Physiology(b.sc)...IIT jam ka kaise preparation lena chahiye..please inform me.....

@prakashpawar1503 4 жыл бұрын

Nice🔥🔥🔥

@priyapriya.c.g2755 4 жыл бұрын

Thank you souch sir

@shomusbiologyofficial 4 жыл бұрын

You're welcome. Glad to hear that you're getting benefit from my lectures

@arnavdeshpande3764 3 жыл бұрын

Thanks for the Video. Please check your calculations before uploading...

@sohilausamarabie9631 3 жыл бұрын

THANK YOU!!!!

@shomusbiologyofficial 3 жыл бұрын

You're welcome. Glad to hear that you're getting benefit from my lectures

@randhirkumarbt 3 жыл бұрын

pls make a video to calculate the net charge and PI (isoelectric point) of a polypeptide at different pH. Problem N0.- 11 of Lehninger biochemistry, chapter -3, 4th edition

@diksha_vlogs_N_shorts 3 жыл бұрын

Confused in the last one ph 12...why is it - 1? It should be -1 +(-1) = -2

@suro-mallar8031 4 жыл бұрын

In case of titration of a peptide chain, should we start from the fully protonated form of the peptide or the fully deprotonated form?

@umaverma9437 2 жыл бұрын

The pI of glycine is written as 5.97 in books. Is that correct if we take ph 5.97 instead of 7 in this calculation? In books the formula for pI is , 1/2 (pK1+ pK2) i.e., 1/2 (2.34+ 9.6) = 5.97

@aradhanagangwar7429 3 жыл бұрын

Here in the structure of glycine why we are not considering NH3+while for COOH we are writing in ionized form

@arnavdeshpande3764 3 жыл бұрын

There should be a Positive Charge over NH . If we consider that, all the answers change. But concept explanation is spot on

@shomusbiologyofficial 3 жыл бұрын

No. The charge depends on pH

@arnavdeshpande3764 3 жыл бұрын

@@shomusbiologyofficial Oh Alright... thanks

@jcash409 4 жыл бұрын

The amine group is H2N deprotonated and H3N+ protonated not H2N+

@rony12007 2 жыл бұрын

An amino acid residue side chain is deprontonated at pH9.5. What is the pKa of this residue if it is fully deprotonated at this pH?

@somanshishukla6929 5 жыл бұрын

Sir why at ph 12 the ans is not - 2 bcz at ph 7 we took - 1 for carboxyl grp in our calculation

@gargi8127 4 жыл бұрын

Exactly, I have the same question

@kashifazaidi3885 2 жыл бұрын

But nh3 was already protonated ... Charge at pH 1 should be 2 ... Plz correct me if I m wrong

@elvishernanserrano7736 Жыл бұрын

Where I can find the second video for polypeptide?

@vanzanmecl5166 Жыл бұрын

since the net charge is zero originally, wouldn't the net charge be 2+ after protonation on both ends not +1 since its's zero plus 2 protons?

@ivanjurkovic9088 4 жыл бұрын

Good afternoon. How many moles of NaOH per mole of histidine should be added to increase 6.5 to 9 pk are given. What will be the charge of histidine at the given pH values, how will it move during electrophoresis? Thanks in advance for your answer.pk1 = ¸3.1, PK2 = 6, PK3 = 8