Great video, thanks for sharing. One of the few I've seen about the actual workflow of CRISPR edits.
@dudehere9643 жыл бұрын
Could you recommend any other KZfaq videos that have proper explanation of the protocol?
@divyathiagarajan90324 жыл бұрын
A very informative Video. Thank you for clearly explaining, in a easily understandable manner for a beginner like me.
@animatedbiologywitharpan4 жыл бұрын
Please share among your friends and help me to reach big audience
@calvincertainrubaya42884 жыл бұрын
Animated biology With arpan how can I get crisp case 9
@williamwallace34463 жыл бұрын
Nice demonstration. My question is why not label every plasmid (sgRNA, Cas9)) with different fluorescent protein (green yellow, red, etc) and sort the cells with two fluorescent proteins and expand from there ? This saves time and effort ! Also you can use antibiotic resistance.
@MridulKaimal Жыл бұрын
Pretty nice comment. which steps exactly would you be doing this ? and is it more economical than this method ?
@TheRealKitWalker3 жыл бұрын
Very nicely presented. Thanks.
@animatedbiologywitharpan3 жыл бұрын
Please share my channel link with your friends and help me to reach big audience
@TheRealKitWalker3 жыл бұрын
@@animatedbiologywitharpan Most definitely. Your channel content is really interesting and informative. ✌️✌️👏👏
@Tennyhu Жыл бұрын
Great information
@animatedbiologywitharpan Жыл бұрын
In order to make high quality content consistently, we need support from you. Please support us by using super thanks option. Super thanks icon is present below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for us.
@mohammadmubarak19373 жыл бұрын
Great informative video!! made it very simple and easy to digest.. cheers!
@animatedbiologywitharpan3 жыл бұрын
Please share my channel link with your friends and help me to reach big audience
@teensharksstartupschool95103 жыл бұрын
at around 9:48 you said it is tedious. How long does it take to run PCR on all three "target colonies". What part is the most repetitious and tedious?
@animatedbiologywitharpan3 жыл бұрын
thanks for the question in reality there might be 300 clones (not 3) which you have to screen for identifying your mutant.....so it becomes hectic in my opinion.
@teensharksstartupschool95103 жыл бұрын
@@animatedbiologywitharpan You have provided one clear video. thanks. I have a question about your answer. When you say "3 clones" are you referring to the three plasmids in the video? If that is the case, I would imagine multiple 96 well plates would be needed to yield one cell line. How long would the entire process take (idea to success?) for a two grad student team?
@pritomc47882 жыл бұрын
Many thanks for your video. Could you please share with us which tools did you use to make this animation?
@animatedbiologywitharpan2 жыл бұрын
I use PowerPoint only
@pritomc47882 жыл бұрын
@@animatedbiologywitharpan Thanks again, for your reply!
@hydermir4237 Жыл бұрын
he has used NCH software
@latinadna3 жыл бұрын
Hey Arpan! I really like your graphics! Where do you find them? Especially the ones in slides 4-6?
@animatedbiologywitharpan3 жыл бұрын
I have done it in Adobe illustrator then used photoshop....but believe me same graphics can be generated using Microsoft power point
@animatedbiologywitharpan3 жыл бұрын
I provide tution for this as well over zoom
@animatedbiologywitharpan3 жыл бұрын
Please share my channel link with your friends and help me to reach big audience
@Ahmad-yv5mo2 жыл бұрын
what are the three ways to upregulate the expression of a gene in arabidopsis using the CRISPR/Case9 method...pease sir help me in this
@animatedbiologywitharpan2 жыл бұрын
Read this article to learn more www.nature.com/articles/s41587-019-0296-7
@charpunk4 жыл бұрын
Gracias!
@animatedbiologywitharpan4 жыл бұрын
Please share among friends and help me to reach big audience
@bhagwatjadhav40894 жыл бұрын
Can you share a paper or source for transcriptional activation repression of RNA by crispr cas9.
@Lily-70458 ай бұрын
I have few question How far we are from these technologies, i mean precision medicine? Who will able to afford it rich or poor? The way climate changes occuring so fast will it be able to inbuilt these precision medicine in particular individual? To make precision medicine how much time is needed for one person, average time?
@animatedbiologywitharpan8 ай бұрын
Quite far from precision medicine
@Lily-70458 ай бұрын
Other answers please Can u answer them Who can afford it? How much average time it will take to make precision medicine for individual person?@@animatedbiologywitharpan
@monerjanala27194 жыл бұрын
At what stage of cell can this Technology be used?
@animatedbiologywitharpan4 жыл бұрын
Cells that are growing
@catarinabarbosa94713 жыл бұрын
Hi!! Could this technique be possible to use in photoreceptor cones?
@animatedbiologywitharpan3 жыл бұрын
Good question .....photoreceptors are differentiated cell so doing crispr is bit difficult...but doing crispr editing in stem cell then differentiating them into photoreceptor is easily doable
@catarinabarbosa94713 жыл бұрын
@@animatedbiologywitharpan That makes sense! I have some more questions if you could help me 😅 So in university we were asked to 'build' a gene therapy and we thought about doing gene editing with Crispr ex vivo. We would collect capillary cells and reprogram them into stem cells, then do the gene editing with Crispr, then differentiate them into photoreceptor cones and finally transplant these cells into the patient eye (the patient had photoreceptor cones degeneration because of a CNGB3 mutation). Is this transplatation even possible?
@animatedbiologywitharpan3 жыл бұрын
@@catarinabarbosa9471 my email is arpanparichha1994@gmail.com ...feel free to connect
@catarinabarbosa94713 жыл бұрын
@@animatedbiologywitharpan Done so! Thanks!!
@animatedbiologywitharpan3 жыл бұрын
I have replied all ready, if you like the content please share with your friends and help me to reach big audience
@LAmourDeViva3 жыл бұрын
So what are the long term effects....will it reverse, mutated or evolve in the future?
@animatedbiologywitharpan3 жыл бұрын
CRISPR induced mutations are stable
@LAmourDeViva3 жыл бұрын
@@animatedbiologywitharpan Thank you for educating me and replying so quickly!
@animatedbiologywitharpan3 жыл бұрын
Please share my channel link with your friends and help me to reach big audience
@LAmourDeViva3 жыл бұрын
@@animatedbiologywitharpan Already have done soo!!
@newhorizons9032 жыл бұрын
@@animatedbiologywitharpan ..... how do you know ... ??... monkeys playing with bombs....
@ZekeSage34 жыл бұрын
Great channel
@animatedbiologywitharpan4 жыл бұрын
Please share my channel link with your friends and help me to reach big audience
@adeelmehmood74392 жыл бұрын
@@animatedbiologywitharpan Crispr-cas9 can cut faulty gene in genetic genes disease like polycystic kidney disease sacs of fluid. Thanks
@ayeshamasood52633 жыл бұрын
what chemicals are used?
@directioner28703 жыл бұрын
Can it cure autoimmune diseases ?
@haileybaker85427 ай бұрын
I know this is an old comment but there is currently cutting edge research delving into exactly this. Yes, auto-immune diseases are due to a genetic factor in immune cells, which causes them to fight the body. CRISPR and gene therapy have already been used in humans with autoimmune disease with success! Though it is still in the research stage. This is a very rough explanation as I am not a gene scientist.
@arthas99574 жыл бұрын
Good job
@animatedbiologywitharpan4 жыл бұрын
Please share my channel link with your friends and help me to reach big audience
@javierespinola3 жыл бұрын
which microscopy are you using?
@animatedbiologywitharpan3 жыл бұрын
Zeiss discovery sterozoom fluorescence microscope is very good for the screening purpose
@priyangshubagchi87782 жыл бұрын
Dada I'm from, kolkata and I am in class 10th, I'm looking forward to start a startup based on Crispr... So I wanted to ask you. Is this idea good??
@animatedbiologywitharpan2 жыл бұрын
Yes why not...it's going to bloom in future
@Priyuge3 жыл бұрын
When we expect this treatment for muscular dystrophy treatment
@animatedbiologywitharpan3 жыл бұрын
It's a long path as of now
@Priyuge3 жыл бұрын
@@animatedbiologywitharpan any treatment for limb girdle muscular dystrophy treatment sir in the world with any cost
@above7846 Жыл бұрын
Спасибо!
@vladonisor70264 жыл бұрын
make my hair grow again??
@dasdasdsdasdasdasd3633 жыл бұрын
I can't understand his accent. ;)
@adeelmehmood74392 жыл бұрын
Following
@animatedbiologywitharpan2 жыл бұрын
Please share my channel link with your friends and juniors
@HUMAIRAKHAN_19996 ай бұрын
Jesus is the prophet of God not son of god
@skittle58889 ай бұрын
can i become spider man by genetically modify myself??????
@animatedbiologywitharpan9 ай бұрын
No
@SherriP2 жыл бұрын
Jesus is the son of God who died and rose three days later so you can be free forgiven healed delivered saved baptized and so much more. Believe in Him as the son of God and you are saved eternally and will live eternally. He's awesome and wants us knowing Him and spending time with Him. Ask anything in Jesus name and He will do it.
@haileybaker85427 ай бұрын
What does that have to do with the video?
@SherriP7 ай бұрын
@@haileybaker8542 no offense but I can post a comment about anything I want to say where I want to say it. Freedom of speech allows me and you and everyone that same right. Also Christians are supposed to evangelize the bible everywhere and this was part of my everywhere the day I wrote it. Everyone needs Yeshua and needs to know about God..there's more to God than my comment. God bless you 🙏💖