CRISPR Cas9 : How CRISPR can be performed in the lab ?

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CRISPR Cas9 : How CRISPR can be performed in the lab ?

Рет қаралды 89,215

Animated biology With arpan

Күн бұрын

Пікірлер: 75

@jamesdillard 4 жыл бұрын

Great video, thanks for sharing. One of the few I've seen about the actual workflow of CRISPR edits.

@dudehere964 3 жыл бұрын

Could you recommend any other KZfaq videos that have proper explanation of the protocol?

@divyathiagarajan9032 4 жыл бұрын

A very informative Video. Thank you for clearly explaining, in a easily understandable manner for a beginner like me.

@animatedbiologywitharpan 4 жыл бұрын

Please share among your friends and help me to reach big audience

@calvincertainrubaya4288 4 жыл бұрын

Animated biology With arpan how can I get crisp case 9

@williamwallace3446 3 жыл бұрын

Nice demonstration. My question is why not label every plasmid (sgRNA, Cas9)) with different fluorescent protein (green yellow, red, etc) and sort the cells with two fluorescent proteins and expand from there ? This saves time and effort ! Also you can use antibiotic resistance.

@MridulKaimal Жыл бұрын

Pretty nice comment. which steps exactly would you be doing this ? and is it more economical than this method ?

@TheRealKitWalker 3 жыл бұрын

Very nicely presented. Thanks.

@animatedbiologywitharpan 3 жыл бұрын

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@TheRealKitWalker 3 жыл бұрын

@@animatedbiologywitharpan Most definitely. Your channel content is really interesting and informative. ✌️✌️👏👏

@Tennyhu Жыл бұрын

Great information

@animatedbiologywitharpan Жыл бұрын

In order to make high quality content consistently, we need support from you. Please support us by using super thanks option. Super thanks icon is present below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for us.

@mohammadmubarak1937 3 жыл бұрын

Great informative video!! made it very simple and easy to digest.. cheers!

@animatedbiologywitharpan 3 жыл бұрын

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@teensharksstartupschool9510 3 жыл бұрын

at around 9:48 you said it is tedious. How long does it take to run PCR on all three "target colonies". What part is the most repetitious and tedious?

@animatedbiologywitharpan 3 жыл бұрын

thanks for the question in reality there might be 300 clones (not 3) which you have to screen for identifying your mutant.....so it becomes hectic in my opinion.

@teensharksstartupschool9510 3 жыл бұрын

@@animatedbiologywitharpan You have provided one clear video. thanks. I have a question about your answer. When you say "3 clones" are you referring to the three plasmids in the video? If that is the case, I would imagine multiple 96 well plates would be needed to yield one cell line. How long would the entire process take (idea to success?) for a two grad student team?

@pritomc4788 2 жыл бұрын

Many thanks for your video. Could you please share with us which tools did you use to make this animation?

@animatedbiologywitharpan 2 жыл бұрын

I use PowerPoint only

@pritomc4788 2 жыл бұрын

@@animatedbiologywitharpan Thanks again, for your reply!

@hydermir4237 Жыл бұрын

he has used NCH software

@latinadna 3 жыл бұрын

Hey Arpan! I really like your graphics! Where do you find them? Especially the ones in slides 4-6?

@animatedbiologywitharpan 3 жыл бұрын

I have done it in Adobe illustrator then used photoshop....but believe me same graphics can be generated using Microsoft power point

@animatedbiologywitharpan 3 жыл бұрын

I provide tution for this as well over zoom

@animatedbiologywitharpan 3 жыл бұрын

Please share my channel link with your friends and help me to reach big audience

@Ahmad-yv5mo 2 жыл бұрын

what are the three ways to upregulate the expression of a gene in arabidopsis using the CRISPR/Case9 method...pease sir help me in this

@animatedbiologywitharpan 2 жыл бұрын

Read this article to learn more www.nature.com/articles/s41587-019-0296-7

@charpunk 4 жыл бұрын

Gracias!

@animatedbiologywitharpan 4 жыл бұрын

Please share among friends and help me to reach big audience

@bhagwatjadhav4089 4 жыл бұрын

Can you share a paper or source for transcriptional activation repression of RNA by crispr cas9.

@Lily-7045 8 ай бұрын

I have few question How far we are from these technologies, i mean precision medicine? Who will able to afford it rich or poor? The way climate changes occuring so fast will it be able to inbuilt these precision medicine in particular individual? To make precision medicine how much time is needed for one person, average time?

@animatedbiologywitharpan 8 ай бұрын

Quite far from precision medicine

@Lily-7045 8 ай бұрын

Other answers please Can u answer them Who can afford it? How much average time it will take to make precision medicine for individual person?@@animatedbiologywitharpan

@monerjanala2719 4 жыл бұрын

At what stage of cell can this Technology be used?

@animatedbiologywitharpan 4 жыл бұрын

Cells that are growing

@catarinabarbosa9471 3 жыл бұрын

Hi!! Could this technique be possible to use in photoreceptor cones?

@animatedbiologywitharpan 3 жыл бұрын

Good question .....photoreceptors are differentiated cell so doing crispr is bit difficult...but doing crispr editing in stem cell then differentiating them into photoreceptor is easily doable

@catarinabarbosa9471 3 жыл бұрын

@@animatedbiologywitharpan That makes sense! I have some more questions if you could help me 😅 So in university we were asked to 'build' a gene therapy and we thought about doing gene editing with Crispr ex vivo. We would collect capillary cells and reprogram them into stem cells, then do the gene editing with Crispr, then differentiate them into photoreceptor cones and finally transplant these cells into the patient eye (the patient had photoreceptor cones degeneration because of a CNGB3 mutation). Is this transplatation even possible?

@animatedbiologywitharpan 3 жыл бұрын

@@catarinabarbosa9471 my email is arpanparichha1994@gmail.com ...feel free to connect

@catarinabarbosa9471 3 жыл бұрын

@@animatedbiologywitharpan Done so! Thanks!!

@animatedbiologywitharpan 3 жыл бұрын

I have replied all ready, if you like the content please share with your friends and help me to reach big audience

@LAmourDeViva 3 жыл бұрын

So what are the long term effects....will it reverse, mutated or evolve in the future?

@animatedbiologywitharpan 3 жыл бұрын

CRISPR induced mutations are stable

@LAmourDeViva 3 жыл бұрын

@@animatedbiologywitharpan Thank you for educating me and replying so quickly!

@animatedbiologywitharpan 3 жыл бұрын

Please share my channel link with your friends and help me to reach big audience

@LAmourDeViva 3 жыл бұрын

@@animatedbiologywitharpan Already have done soo!!

@newhorizons903 2 жыл бұрын

@@animatedbiologywitharpan ..... how do you know ... ??... monkeys playing with bombs....

@ZekeSage3 4 жыл бұрын

Great channel

@animatedbiologywitharpan 4 жыл бұрын

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@adeelmehmood7439 2 жыл бұрын

@@animatedbiologywitharpan Crispr-cas9 can cut faulty gene in genetic genes disease like polycystic kidney disease sacs of fluid. Thanks

@ayeshamasood5263 3 жыл бұрын

what chemicals are used?

@directioner2870 3 жыл бұрын

Can it cure autoimmune diseases ?

@haileybaker8542 7 ай бұрын

I know this is an old comment but there is currently cutting edge research delving into exactly this. Yes, auto-immune diseases are due to a genetic factor in immune cells, which causes them to fight the body. CRISPR and gene therapy have already been used in humans with autoimmune disease with success! Though it is still in the research stage. This is a very rough explanation as I am not a gene scientist.

@arthas9957 4 жыл бұрын

Good job

@animatedbiologywitharpan 4 жыл бұрын

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@javierespinola 3 жыл бұрын

which microscopy are you using?

@animatedbiologywitharpan 3 жыл бұрын

Zeiss discovery sterozoom fluorescence microscope is very good for the screening purpose

@priyangshubagchi8778 2 жыл бұрын

Dada I'm from, kolkata and I am in class 10th, I'm looking forward to start a startup based on Crispr... So I wanted to ask you. Is this idea good??

@animatedbiologywitharpan 2 жыл бұрын

Yes why not...it's going to bloom in future

@Priyuge 3 жыл бұрын

When we expect this treatment for muscular dystrophy treatment

@animatedbiologywitharpan 3 жыл бұрын

It's a long path as of now

@Priyuge 3 жыл бұрын

@@animatedbiologywitharpan any treatment for limb girdle muscular dystrophy treatment sir in the world with any cost

@above7846 Жыл бұрын

Спасибо!

@vladonisor7026 4 жыл бұрын

make my hair grow again??

@dasdasdsdasdasdasd363 3 жыл бұрын

I can't understand his accent. ;)

@adeelmehmood7439 2 жыл бұрын

Following

@animatedbiologywitharpan 2 жыл бұрын

Please share my channel link with your friends and juniors

@HUMAIRAKHAN_1999 6 ай бұрын

Jesus is the prophet of God not son of god

@skittle5888 9 ай бұрын

can i become spider man by genetically modify myself??????

@animatedbiologywitharpan 9 ай бұрын

@SherriP 2 жыл бұрын

Jesus is the son of God who died and rose three days later so you can be free forgiven healed delivered saved baptized and so much more. Believe in Him as the son of God and you are saved eternally and will live eternally. He's awesome and wants us knowing Him and spending time with Him. Ask anything in Jesus name and He will do it.

@haileybaker8542 7 ай бұрын

What does that have to do with the video?

@SherriP 7 ай бұрын

@@haileybaker8542 no offense but I can post a comment about anything I want to say where I want to say it. Freedom of speech allows me and you and everyone that same right. Also Christians are supposed to evangelize the bible everywhere and this was part of my everywhere the day I wrote it. Everyone needs Yeshua and needs to know about God..there's more to God than my comment. God bless you 🙏💖

@lelotz 7 ай бұрын

@@SherriPJesus L