CRISPR Gene Editing: Using CRISPR-Cas9 with the Out of the Blue CRISPR Kit

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Күн бұрын

Follow along with this step-by-step walkthrough of the lacZ gene editing laboratory activity in Bio-Rad’s Out of the Blue #CRISPR Kit. Click “Show More” for chapters and free resources. 👇
00:00 - Introduction
01:22 - lacZ in blue-white screening
02:57 - CRISPR-Cas9 gene editing
05:21 - Laboratory protocol overview and background
08:23 - Experimental setup
09:24 - Example Results
09:55 - Instructor Preparation
10:22 - Student workstation setup
11:17 - Student protocol
20:36 - Additional Information
See the first video in this series CRISPR Explained: Gene Editing History, Technology, and Applications at • CRISPR Explained: Gene...
For more videos about bacterial transformation visit the pGLO Bacterial Transformation playlist at • pGLO Bacterial Transfo...
The 2020 Nobel Prize in Chemistry was awarded for CRISPR. Learn more about the Out of the Blue CRISPR Kit and Genotyping Extension, and download free modeling and bioinformatics activities in the instructor and student guides, at bio-rad.com/outoftheblue
Free CRISPR teaching resources at bio-rad.com/teachcrispr
See more curated videos on our CRISPR Gene Editing playlist at • CRISPR Gene Editing
** Know of a KZfaq video or channel helpful to life science students and teachers we could add to our Explorer Video Community? Email explorer@bio-rad.com! **
*** Stay up-to-date with the latest videos added to the Explorer Video Community: subscribe to this KZfaq channel at / @bioradeducation
***
Bio-Rad Explorer's KZfaq channel is a hub of educational and instructional videos from Bio-Rad and the life science education community.
Bio-Rad Explorer synergistically brings about change in hands-on science learning and provides educators with easy-to-use and reliable research-quality kits, equipment, reagents, and curricula, helping educators to stay on the cutting edge. ALL Bio-Rad products used for K-12 and undergraduate classes are eligible for the EDU discount.
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Пікірлер: 43

@Zane-It 2 жыл бұрын

i hope this channel makes more videos on this topic

@prakashkannan7274 3 жыл бұрын

Well explained thanks guys

@Shaq0112 2 жыл бұрын

The best - thank you!

@user-pe4bx9yo4w 3 жыл бұрын

Thank you for the clear explanation💯

@BioRadEducation 3 жыл бұрын

You're welcome! Be sure to check out all of the videos on our CRISPR playlist from Bio-Rad and other KZfaq channels!

@gaby31052000 2 жыл бұрын

Brilliant video

@KrishnaKumar-gs5mg 2 жыл бұрын

superb.

@marc8239 Жыл бұрын

Hey I’d like to make my pet hamster glow in the dark, can I get the kit?

@ProCook703 Жыл бұрын

i want to bioengineer myself

@user-bv5lz7fw5m Жыл бұрын

좋아요!

@adeelmehmood7439 2 жыл бұрын

Good

@mateuszbylewski298 2 жыл бұрын

👏

@misterhemp 9 ай бұрын

is there a continuation?

@MT-jb3pw 3 жыл бұрын

Out of the Blue CRISPR Kit and Genotyping Extension is not on your page???

@BioRadEducation 3 жыл бұрын

Hi, MT - you can learn more at www.bio-rad.com/outoftheblue

@dianalancaster4968 2 жыл бұрын

What does the heat shock step do?

@BioRadEducation 2 жыл бұрын

Hi, Diana! The heat shock is part of the bacterial transformation step used to incorporate a plasmid, either pLZDonor or pLZDonorGuide, into the cell. For more info, visit bio-rad.com/teachCRISPR

@solcarmo 3 жыл бұрын

What is in the transformation solution?

@BioRadEducation 3 жыл бұрын

Hi, Solange! Transformation Solution is a sterile CaCl2 solution. You can learn more about the bacterial transformation and the role of transformation solution from our family of pGLO bacterial transformation kits: visit bio-rad.com/pGLO and bio-rad.com/teachpGLO for more info. The manuals discuss the role of transformation solution in plasmid transformation which, for the Out of the Blue CRISPR Kit, is the method in which the plasmids pD and pDG are introduced into the cells.

@HewanDemissie 2 жыл бұрын

I live in Ethiopia. This kit will be used to teach students about the CRISPR/Cas9 gene-editing technology. Is it possible for me to obtain it? If so, from what source? Thank you for the interesting presentation.

@BioRadEducation 2 жыл бұрын

Hi, Hewan! Let's continue this conversation - please send us your request at bio-rad.com/ExplorerHelp

@chitrajawalanj7756 3 жыл бұрын

could you explain the results please?

@BioRadEducation 3 жыл бұрын

Hi, Chitraja! Check out the FREE student and instructor manuals at bio-rad.com/outoftheblue for additional information and help to understand the experiment.

@chitrajawalanj7756 3 жыл бұрын

@@BioRadEducation it is coming that this content is not available in my region.

@BioRadEducation 3 жыл бұрын

Hi, Chitraja! Please send an email to us at explorer@bio-rad.com and we'll help you out. Thanks!

@srinivass442 Жыл бұрын

Lol I guess two blue, one white, one dead

@uroojamjad2128 7 ай бұрын

is this pseudomonas aeruginosa?

@neo-filthyfrank1347 3 ай бұрын

did you even watch the video

@Karen-jj4fr 3 жыл бұрын

Can I use this to knock out myostatin gene

@BioRadEducation 3 жыл бұрын

Hi, Karen! The kit only knocks out the lacZ gene in the bacterial chromosome. It is for educational use to teach CRISPR gene editing and isn’t able to be adapted to knock out other genes.

@pipony8939 2 жыл бұрын

@@BioRadEducation what tools able to knock out then?

@BioRadEducation 2 жыл бұрын

Hi,@@pipony8939! A different guide RNA sequence would be needed to target a gene other than lacZ. The Out of the Blue CRISPR Kit only targets the bacterial lacZ gene, as the guide RNA sequence is for that of lacZ.

@pipony8939 2 жыл бұрын

@@BioRadEducation what is the reason that for each target you need a different guide RNA sequence?

@BioRadEducation 2 жыл бұрын

Hi, @@pipony8939! Yes: each guide RNA is specific for the DNA sequence of the target. Thus, if you wanted to target a different gene you would use a different guide RNA, and that guide RNA would have a specific sequence that matches the desired target gene. You can watch and learn about this and how CRISPR-Cas9 gene editing works in our CRISPR Explained: Gene Editing History, Technology, and Applications video at kzfaq.info/get/bejne/qrSflqSUmrqvpqs.html starting around 15min and 40seconds of that video.