Benedict's Test for Reducing Sugars || Benedict's Test for Carbohydrate || Biochemistry Practical

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4 жыл бұрын

Benedict's Test - This is the video on benedict's test which done for the detection of reducing sugars present in the urine.
Video contains Principle of Benedict's Test, Procedure, Discussion of viva questions and Demonstration of benedict's test.
Reducing Sugars - 00:28 minutes
Benedict's Test Principle - 00:52 minutes
Benedict's Test for Glucose, Sucrose and Fructose - 01:49 minutes
Color changes seen in benedict's test - 02:13 minutes
Composition of benedict's reagent - 02:38 minutes
Benedict's test procedure - 03:06 minutes
Benedict's test is a semi quantitative test - 03:27 minutes
False positive benedict's test - 04:55 minutes
Demonstartion of benedict's test - 05:33 minutes
All reducing sugars give positive benedict’s test.
Principle - Reducing sugars under hot alkaline conditions tautomerize and form enediols which are powerful reducing agents.
They reduce the cupric ions of Benedict’s reagent to red cuprous oxide.
Enediol is the enol form of sugar because two OH groups are attached to the double bonded carbon
Color Changes from Blue to Green to Yellow to Orange to brick red if there is presence of reducing Sugar. Sucrose will give negative benedict’s test
Benedict’s qualitative reagent contains:
1. Copper sulfate: Furnishes cupric ions (Cu++) in solution.
2. Sodium carbonate: Provides alkaline medium.
3. Sodium citrate: It acts as a preservative.
Procedure -
5 ml of Benedict’s reagent + 5-8 drops of solution
Apply heat to it for 1-2 minutes and observe
the color changes
#glucosuria #sugarinurine #benedict's #benedicttest #biochemistrybasicsbydramit #biochemistrypractical #diabetes

Пікірлер: 58

@preranarana2429 3 жыл бұрын

Thank you so much sir... this video has demonstration,viva question n also explanation... it's too much helpful

@Biochemistrybasics 3 жыл бұрын

My pleasure prerana. My all practical videos are in that format. Please spare a few minutes and do spread the word about the channel among your batchmates and friends

@preranarana2429 3 жыл бұрын

@@Biochemistrybasics yes sir...it is needed too much now because due to pandemic we r joining online classes of MBBS 1st year..so for making Clear concept of these tests we need some laboratory like feeling and that we can get from your valuable videos..thank you so much sir

@Biochemistrybasics 3 жыл бұрын

@@preranarana2429 thanks for the kind words 😊✌️

@preranarana2429 3 жыл бұрын

@@Biochemistrybasics Feeling blessed to have your kind reply..sir I have cleared NEET 2020 and taken admission in a gov medical college but due to pandemic n online classes some prblms , doubts are here . That's why I m watching KZfaq videos and there your videos are the best one..I m following your videos and I ll follow your videos from today only..they are much helpful..thank you once again.

@Biochemistrybasics 3 жыл бұрын

@@preranarana2429 I try my best to reply each and every comment from my students. There are more than 200 videos on Biochemistry with NEET PG MCQS. So you can have idea regarding neet pg exam too. Just started video series on Chemistry part. All important practical videos are also there.

@shitalnakiya1513 4 жыл бұрын

Thank you sir For making video on laboratory It's helpful at last on exam for revise procedure of all test

@Biochemistrybasics 4 жыл бұрын

Yes shital. We will try to cover important tests and practical. Please spread the word 😊

@Someoneunknownduck Жыл бұрын

Thankyou so much!! You explain everything amazingly. More power to you🤍🤍🤍

@Biochemistrybasics Жыл бұрын

Thanks a lot

@sumanbarua6455 2 жыл бұрын

Keep going, We are waiting for ur more videos...👌

@Biochemistrybasics 2 жыл бұрын

Most welcome dear

@madhuribhatt3068 4 жыл бұрын

Thank you sir,practical video give our,revise practical so easy performs test in exam🙏

@Biochemistrybasics 4 жыл бұрын

Yes Madhuri. Plus viva questions will help you in exam.

@yinban4977 3 жыл бұрын

Thank you for posting this video. After watching this l clear my doubt.🙏🙏🙏

@Biochemistrybasics 3 жыл бұрын

Glad to hear that. 😇🙏

@yinban4977 3 жыл бұрын

@@Biochemistrybasics As I am A teacher newly handle for higher class I have some doubt in practical. Thats why I watch your video really very clear and simple.

@Biochemistrybasics 3 жыл бұрын

@@yinban4977 thanks mate. I am sure you will do full justice to the new responsibilities given to you. Congratulations and All the very best. 😊🎊

@yinban4977 3 жыл бұрын

@@Biochemistrybasics Thank you. And I am Captain veeranganai

@gulfwarveteran8528 4 жыл бұрын

Thanks sir made my day

@Biochemistrybasics 4 жыл бұрын

😇🙏

@dhanusreeanjuri3233 3 жыл бұрын

Nice

@Biochemistrybasics 3 жыл бұрын

Thanks

@user-jm2es6ud2v Жыл бұрын

Sir please make videos on fat test by SUDAN IV , please tomorrow is my practical exam

@ravinachoudhary4457 Жыл бұрын

Very nice sir 👏 in viva teacher asked me why 5 ml benedict reagent and 8 drop urine is taken?? Can u please explain

@Biochemistrybasics Жыл бұрын

Thanks Ravina. Why in Benedict test five ml of reagents and 8 drops of urine taken The reason why five milliliters of Benedict's reagent and eight drops of urine are used in the test is to maintain consistency and standardization in the procedure. Using a fixed volume of reagent ensures that the test is conducted under the same conditions each time, which is important for obtaining reliable results. Similarly, the use of a fixed number of drops of urine helps to ensure that the amount of reducing sugar in the sample is within the detection range of the test. Too much or too little urine can skew the results and make it difficult to interpret them accurately. Overall, the use of fixed volumes and amounts in the Benedict's test is important for ensuring that the test is performed accurately and consistently, and that the results obtained are reliable and meaningful. Hope it helps

@ravinachoudhary4457 Жыл бұрын

Thank you sir for replying 🥰❤️

@youtube.crea116 3 жыл бұрын

Sir general scheme for identification test for carbohydrate kindly explain

@Biochemistrybasics 3 жыл бұрын

It's molish test. All carbohydrates except glyceraldehyde will give molish test positive

@Biochemistrybasics 3 жыл бұрын

Link for it is kzfaq.info/get/bejne/sK6ToNtzzsXbd2Q.html

@navitameena6832 3 жыл бұрын

Iodine test, barfoed test, osazone test bhi upload kijiye or unknown carbohydrates ka bhi plz sir Ji

@Biochemistrybasics 3 жыл бұрын

Sure

@navitameena6832 3 жыл бұрын

@@Biochemistrybasics thankyou so much sir ji

@youtube.crea116 3 жыл бұрын

Sir our 1st year public exam on 22nd kindly help us to study whole subjects?

@Biochemistrybasics 3 жыл бұрын

I have made 200 + videos. All are posted in respective playlist. Go through it. It will help you for sure

@marcusdonnreynunez9060 2 жыл бұрын

sir how will we know the degree of reducing power based on the results?

@Biochemistrybasics 2 жыл бұрын

That you can't say.... Only thing you can say whether sugar is reducing or not

@prathmeshteli3057 6 ай бұрын

Benedict's reagent react with reducing sugar color get yellow,.why?

@Biochemistrybasics 6 ай бұрын

The alkaline sodium carbonate present converts the sugar into a strong reducing agent called enediols. Due to the creation of cuprous oxide Cu2o during the reduction reaction, the mixture's colour changes from blue to brick-red precipitate