AP Biology Lab 6: Molecular Biology

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Paul Andersen explains the two major portions of the molecular biology lab in AP Biology. He starts by discussing the process of transformation. He explains how you can use the pGLO plasmid to produce glowing E. coli bacteria. He then describes how you can use restriction enzymes and the process of gel electrophoresis to cut and separate DNA.
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@bridgettestarrmayse 6 жыл бұрын

THESE VIDEOS LITERALLY SAVE MY BIO GRADE LIKE I DONT UNDERSTAND THE WHOLE WEEK IN MY CLASS BUT BAM 8 MINUTES VIDEO AND I KNOW AND UNDERSTAND EVERYTHING THANK YOU

@mahdighasemi9294 2 жыл бұрын

honestly. I felt that on a spiritual level.

@303404jb 7 жыл бұрын

If i ever win a nobel prize i'm gonna give you a shout out, like before my mum even

@rittenbrake1613 5 жыл бұрын

LOL !!! can u give me a shout out too as I m supporting u in the comment session

@vagueequinox7543 4 жыл бұрын

@@rittenbrake1613 yeet

@pinkk4757 9 жыл бұрын

I didn't have an interpreter on the day I did this lab in my class, and I was so lost! I was nervous to find out that this process was going to be on exam! Yikes! However, I finally understand thank you! The closed caption helped me significantly, again thank you SO MUCH! :)

@kelseyshea96 7 жыл бұрын

You seriously cover all of the material that I have googled for my nursing prerequisite classes. Thanks! These are such a big help.

@xOpIaNoGuRlxO 10 жыл бұрын

I swear my teacher comes to class half stoned every day, he has no clue how to teach.

@EthanIverson 10 жыл бұрын

Chamblee?

@rittenbrake1613 5 жыл бұрын

steal all his weed

@lilygurllovemuzic 4 жыл бұрын

lmfaooo

@brittneysellers4329 2 жыл бұрын

Greetings! Your videos are awesome even after so many years. It would be amazing if you did an updated series!!

@tasiakwaa 9 жыл бұрын

Thank you!!!!!!!! I wish you were my micro professor. You made my night learning pglo a lot easier

@129missJessica 11 жыл бұрын

Thank you so much you seem to make everything simple to me! I always check your page to see if you have a video on all my science problems!

@hunterhunter1477 3 жыл бұрын

You are amazing. One of the worst things about online school is labs, and you got the best solution for it. YOU ARE THE BEST!!!

@MaggieRyan95 11 жыл бұрын

This video was awesome. I spent a week on this with my IB Bio 2 class and the easy diagrams really helped explain what I was doing in the labs. Thanks, I wish you were my professor!

@kbtgu 10 жыл бұрын

I have these two lab reports due tomorrow and this video just saved my life THANK YOU

@Stinakat 11 жыл бұрын

I'm really grateful to have found this...It's going to be my first year teaching AP bio, so your videos are very helpful, and answer many of my questions that I have from reading the manuals...

@AshrZ 3 жыл бұрын

Well, give us an update 7 years later! How is it going?

@mahdighasemi9294 2 жыл бұрын

@@AshrZ lmao

@claudiablanco2597 9 жыл бұрын

omg YOURE SUCH A GOOD TEACHER.

@ioriok_KeanUniversity 5 жыл бұрын

Thank you Mr. Andersen!

@TheMFJonez 9 жыл бұрын

this man has saved my life countless times

@10monicamaharani95 11 жыл бұрын

Your videos are always helpful! Thanks a lot! :)

@lovinreginald 7 жыл бұрын

just filled like 5 pages of my lab notebook. thanks brotha!

@xosabrina47 10 жыл бұрын

I love this guy! I have a Lab test today on 6 labs and we didn't have to hand in this lab so I have no idea what it was about :) Now I do :D

@MoneyTreeCare 9 жыл бұрын

Awesome! Thanks for posting it... I started to do videos too, but never know if I can measure up to the better stuff out there lol!

@giggy2402 5 жыл бұрын

I take an online AP bio class and I have hardly learned anything this year cuz we don’t have a teacher, just a procter, so this was very very helpful

@mollybennett312 4 жыл бұрын

This is so helpful! Thank you!

@alipinhasi2206 9 жыл бұрын

I'm taking college genetics. We were focusing on this topic. This has a very good explanation for what is going on. Thank you!

@daggie1747 6 жыл бұрын

Can you do a video on plasmids and transposons. You make it so easy to understand what I am reading. Thank you in advance

@phoenixwings6441 10 жыл бұрын

thank you for posting this. I get nervous about labs so I always try to find videos that show me how to do stuff because i get scared of all the stuff i have to do !!

@MultiNerve 10 жыл бұрын

Hope you guys pursue biology as a major/minor in college, because, in high school, you study the experiments and what happens, as well as what we now understand. But in college, you get to actually perform these experiments and reinforce what you studied in high school and relearned in college!

@honeykai8274 8 жыл бұрын

I love you so much. I actually understand this now

@PreetKaur-fk3ds 5 жыл бұрын

Indeed extremely helpful. Hi hanks a million

@kanizzehra1 10 жыл бұрын

Oh Lord! thanku so much!!!

@dilemmadilemmasen6216 10 жыл бұрын

We did this in class with 5 groups. Everyone got it to transform!

@jasonwupilly1997 11 жыл бұрын

very high quality lesson.

@groovyzombietingz4804 6 жыл бұрын

I HAVE A LAB REPORT DUE TONIGHT THANK YOU SO FRIKIN MUCH

@yihui7176 8 жыл бұрын

thanks i needed this

@amkaoamkao6359 5 жыл бұрын

you are the freaking best!

@johnting1189 5 жыл бұрын

1:20 . For some reason ,for plate 4 , almost 90% of the lawn glow when shined with UV light .. which contract because it is supposed to glow in clusters . So what went wrong ? Anyone know ??

@user-nw9xy7qe3x 7 ай бұрын

I am falling in love with Biology

@minkhant4533 4 жыл бұрын

Kindly, sir. Can you tell me which of these plates are used as control?

@heatherbuckley949 7 жыл бұрын

Mr. Paul Anderson I am sooooooo proud of my classes as 8,yes 8, of 14 groups got their bacteria to glow!!

@Bozemanscience1 7 жыл бұрын

Congrats! Very cool.

@xiiixiiih.16 2 жыл бұрын

Did they feed the bacteria to eat alum limes and potatoes?

@heatherbuckley949 Жыл бұрын

@@xiiixiiih.16 huh?

@xiiixiiih.16 Жыл бұрын

To@@heatherbuckley949 Did the bacteria glow from digesting different foods and preservatives or giant flagitious gases ✌️

@jaredfisse6120 4 жыл бұрын

u r a great man

@amairaniroman 9 жыл бұрын

Wow I actually understand something for once

@lylaylkvs4862 9 жыл бұрын

Amairani Montiel Same XDDD

@tyboucino6572 3 жыл бұрын

u dont lol

@tyboucino6572 3 жыл бұрын

10 ай бұрын

Thank you. Greetings from Bimac Research Group at Universidad del Cauca

@breannecoulthard3274 4 жыл бұрын

What would happen if amp was never added to the +DNA plates?

@xImxWithxYoux1 11 жыл бұрын

LOL finals tomorrow, almost done watching all 1st semester labs :D

@simpimaurya3154 11 жыл бұрын

yep this is somthin really informative

@TheBryanpark1 7 жыл бұрын

k, now let me go fail my bio test :/

@lylaylkvs4862 9 жыл бұрын

Thank You!!!! XD

@dominicrincker8280 3 жыл бұрын

any tips for a successful transformation? our lab groups are competitive lol

@llewgibson 6 жыл бұрын

I viewed your channel mate, absolutely love the content. Subscribed straight away, We should connect!

@spoonfork21 9 жыл бұрын

You look just like my principal whose name is also Mr. Anderson!

@melsbookshelves 12 жыл бұрын

mine didnt work :( but great explination

@yamininikhariya3172 6 жыл бұрын

Can u pls tell me why GFP will not grow if araC (arabinose) is not there..??

@SuperMagicRevealer 5 жыл бұрын

GFP is just a protein, but plasmids are DNA. DNA has to be transcribed into mRNA and then translated into a functional protein. The gene for GFP is useless if there is not a starting point for transcription called a "promoter"--that is the araC that you see in the video. AraC is an inducible promoter, meaning that it is usually off but the presence of some chemical (arabinose) can turn it on. Thus, arabinose will turn on the promoter, which initiates transcription of the GFP gene.

@fivecarsfly 11 жыл бұрын

because they picked up the ampicillin resistant dna (Pglo)

@Kesonjeta 12 жыл бұрын

Why does science make my brain melt! D:

@smiley8106 4 жыл бұрын

Wait how do you know it’s lab 6

@letsameliorate1351 2 жыл бұрын

Hello sir.....ph.d in molecular biology involves animal dissections(like where to extract DNA etc from)too as lam from zoology background....please reply

@letsameliorate1351 2 жыл бұрын

Please reply

@hugoflores7926 10 жыл бұрын

I'm having trouble learning and absorbing information

@Cronorchy 11 жыл бұрын

What do you use to write? Do you just use a mouse? Or is it one of those draw pads?

@jen71264 12 жыл бұрын

haha, same! i just wanna sleep, but i need to finish them tonight

@tanyathep1 4 жыл бұрын

neat.

@mahsabayati491 6 жыл бұрын

greatt

@vergildisparda Жыл бұрын

This is a Certified Chewachong Classic

@vickymendez2505 7 жыл бұрын

are plasmids always beneficial to bacteria?

@SuperMagicRevealer 5 жыл бұрын

Beneficial is relative to the context of the selective pressures of the environment. A common transformation protocol involves the solution mentioned in the video alongside a heat shock, which will shock the bacteria and "trick" them into uptaking dna. What is not mentioned is that bacteria can get rid of useless plasmids during replication, which is why it is important that these plasmids for lab work have a selectable marker (such as one that encodes for resistance to an antibiotic).

@kjebbies6632 8 жыл бұрын

MS.CHIANG IS LIFE.

@Dennis-cc4dx 3 жыл бұрын

I thank you my deer!

@emiliano9523 3 жыл бұрын

@AshrZ 3 жыл бұрын

@lilygurllovemuzic 4 жыл бұрын

god bless, come teach my bio prof how to do biology. thx.

@idegteke 5 ай бұрын

And a fluorescent graffiti of disbelief appears on the castle of nature. I hope it won’t be interpreted as a demolition notice:)

@antoinepham3342 8 жыл бұрын

HIII MS. TAYLOR

@TheBryanpark1 7 жыл бұрын

@donle3032 8 жыл бұрын

Hi Mrs.Taylor

@boblee14 7 жыл бұрын

Don Le lol

@boblee14 7 жыл бұрын

Don Le lol

@AlwaysLoudAA 10 жыл бұрын

last minute cram section!

@Nesvarbu2011 6 жыл бұрын

CaCl2 is a “divalent cation.” Can you explain why a “monovalent cation” like NaCl would not be as effective?

@SuperMagicRevealer 5 жыл бұрын

The reason why divalent cations (basically always Ca++) work is because they can simultaneously be attracted the negatively charged bacterial membranes and the negatively charge DNa. This allows Ca++ to basically bring the DNA closer to the cell. Monovalent cations would only be able to be attracted to one or the other.

@harrythehamster72 5 жыл бұрын

6:00 poor bacteria 😭

@JayFetzer 9 жыл бұрын

5 out of 30 students were successful in growing transformed bacteria? Psshhh. No big deal. My class had 100% of the students successfully grow bacteria that glowed. Again, no big deal. We be swaggy like that.

@richardwu9013 6 жыл бұрын

daaaaaamn

@2ndintelligentWorld 11 жыл бұрын

6:54 lol

@devonbrandt5258 4 жыл бұрын

"interpelate"

@praneelmadhuvanesh3770 3 жыл бұрын

who watches him at 2x speed lol

@crustystalesocks8579 7 жыл бұрын

dude i still can't science😔

@hades_head_empty 7 жыл бұрын

11 dislikes I can't

@MangoDrank 11 жыл бұрын

Damn i hate doing these videos

@xiiixiiih.16 2 жыл бұрын

🍎

@oregonrexy5231 9 жыл бұрын

migrate my bawls-

@oregonrexy5231 7 жыл бұрын

You need to learn how fun it is for your betters to beat up people like you on-line. You go ahead and tell me why a university with 50K student body wants to do a chemical poration when an electro-poration is so much more efficient. u need to tell me!

@TheBryanpark1 7 жыл бұрын

Oregon Rexy I have zero idea

@oregonrexy5231 7 жыл бұрын

Bryan Park That's ok - big thoughts aren't for everyone.