cDNA Library

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Күн бұрын

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Пікірлер: 80

@melis2198 5 ай бұрын

you are saving me. 5 minutes ago I was crying from not being able to understand my molecular cell biology textbook (first year med student), and now it all made sense. thank you. love your videos, please never stop

@Anushka2704 9 жыл бұрын

Thank you so much for making such a complex topic so simple and easy to understand. I wish my textbook explained things the way you do.

@pegboh3315 8 жыл бұрын

extremely clear and very easy to understand. Just to be clear @7:16 did he mean "if we place this cDNA in a prokaryotic cell"

@thachnamxanh 7 жыл бұрын

yeah, I'm also a little confusing at that point. I think it should be "cDNA in a prokaryotic cell"

@greenzebra97 6 жыл бұрын

YEs he made a mistake

@ferengarajbrahma8532 6 жыл бұрын

Pannel Egboh lol, exactly, nonetheless his explanation is the best

@ferengarajbrahma8532 6 жыл бұрын

Best Explaination 💯/💯, chow chow !

@autumnleaves5419 5 жыл бұрын

Yes. By which he meant vectors.

@nyoomi 7 жыл бұрын

Thank you so much! Could not have understood this without your help.

@seenithambyjeevaranjan2735 9 жыл бұрын

Beautifully explained. Thanks very much for your great service.

@telatti 4 жыл бұрын

i absolutely love your lectures! thank you so much for all the hard work you put in. you're saving our lives so we can become doctors and save many more lives!

@emmanuelolajide5281 8 жыл бұрын

Thank you... very easy to grab. Great teacher.

@mahsaadib8779 2 жыл бұрын

You make my life 10 times easier!! thank you thank you thank you❤️❤️❤️❤️

@zainabbaqer4713 7 жыл бұрын

really perfect explanation. your are one of the best lecturers

@pershianemati6668 2 жыл бұрын

THANKOU SO MUCH SIR! only if my lecturers could explain as awesome as you... I love your content and videos it ALWAYS helped me through understanding fully . i hope wherever you are you be always healthy and create more awesome videos. God bless you.

@whatsonmymind4848 7 жыл бұрын

Hey, really good video. One question. In another video about cDNA you said that to separate the DNA/RNA hybrid we have to increase the pH, because at a basic pH RNA gets hydrolysed but DNA not, so we will remain with the sDNA, here you say we heat them up to separate, which makes sense for me, but which of the two methods are right? Or can we use both? Has one more advantage?

@promisendlovu8550 8 жыл бұрын

omg dis is very easy.....................l cant even believe this !!!! thank you very much Sir

@abdshremo4374 8 жыл бұрын

you are awesome man!!!!! what a great work

@marjanm.hashemi5757 8 жыл бұрын

perfect!thank you

@Sakshi-hf3pf 8 жыл бұрын

that was very well explained, thank you. could you please explain subtractive hybridization as well? :)

@ef4902 5 жыл бұрын

Thank you, you are awesome!!!

@farahqaryouti2314 7 жыл бұрын

wow 😍😍😍😍 super clear now!!

@malikbasharat7119 8 жыл бұрын

Very much helpful Sir have u posted lectures on plant physiology..I need them basically.

@Navidmsv 4 жыл бұрын

Very useful like other videos. Thanks.

@lenorasalae.6913 2 жыл бұрын

Thank you sir, vividly explained but i have a little worry. is there a possibility for the sscDNA to be re-transcribe into RNA? And given that DNA polymerase needs a free 3' OH to be able to form the complementary strand or betterstill it needs a primer for elongation to take place. what happens in this case? where does the sscDNA get its primer from?

@arixii3796 7 жыл бұрын

do we need to place our double stranded cDNA inside a vector of a prokaryote? (ie plasmid/lambda phage) or can we just insert the cDNA molecule directly inside a prokaryote??

@karamalfakih9476 2 жыл бұрын

Is there some way to know each individual gene sequence So that we can use a specific restriction enzymes to put the gene in a vector?

@jonelgeneta9590 3 жыл бұрын

Assuming you have determined the sequence of a certain enzyme/protein product, how will you identify its correct DNA sequence (*some codons are redundant or wobbled) using the cDNA libary? just asking.

@akshayas768 Жыл бұрын

Very awesome no words to say

@shoaib349 6 жыл бұрын

Nicely explained. Please make a video on Mitronchondrial Biogenesis

@stanlee5352 7 жыл бұрын

great! but how do u prevent from alternative splicing when producing pre-mRNA into mRNA as a template?

@JoelNHarris 7 жыл бұрын

For stuff that relies on alternative splicing (ex. antibodies) I think you'd only be able to make cDNA for one transcript variant that codes for one specific protein

@biswajit4134 8 жыл бұрын

thank you

@busisiwenzima8797 3 жыл бұрын

great lecture

@sashathomas58 7 жыл бұрын

Out of curiosity I believe at 6.08 there maybe an error, unless I'm not thinking correctly. All eukar dna consists of polyA tails so therefore in order to prepare a cDNA library a poly-T primer is created for complementarity??

@imamsajid2463 5 жыл бұрын

eukaryotic DNA doesn't have a poly A tail, it is the mRNA that does, when the reverse transcription is happening the poly A tail is removed and DNA does not contain poly T tail, therefore you don't need a Poly T primer in order to prepare a cDNA library

@Ssmshakeeb 8 жыл бұрын

If restriction enzymes cleaves by recognition of some specific sequence.. Why does it selectivly cleave genes differently? Isnt there a high chance of braking middle of genes?

@amritasaha6738 4 жыл бұрын

nice class

@sujitkundu6561 6 жыл бұрын

wow beautiful class

@justinalmano8273 3 жыл бұрын

wonderful!!!

@vendolyncher5081 Ай бұрын

thank you i love you

@thu-dungdoan9966 8 жыл бұрын

clear and easy to understand, thanks man

@mmaking8664 7 жыл бұрын

The mRNA strandis 5' to 3'. Shouldn't the complementary DNA strand be 5' to 3' also?

@TimetoWatch247 7 жыл бұрын

No, because then it wouldn't generate the same mRNA transcript due the 5'-3' directionality of DNA polymerase. The cDNA generated is an antisense strand (non-coding) which binds to the sense strand, or coding DNA.

@4dham 7 жыл бұрын

So if I place a DNA inside a prokarytic cell, the prokaryotic cell cannot synthesize the protein, because of the introns sequences the DNA contain?

@leokuo8995 7 жыл бұрын

yes

@MarieBai 4 жыл бұрын

Bravo!!!!

@Ashna.sawa22 2 жыл бұрын

Thanks

@lysannbock5608 5 жыл бұрын

Wow thanks man

@ST-gd4eq 8 жыл бұрын

Amazing video, but I think there may have been some errors. Please correct me if I am wrong. 1. On the bottom right, the cDNA is shown to be 5' to 3' and the mRNA is also shown to be 5' to 3', should the cDNA actually be 3' to 5' so that is is complementary to the mRNA? 2. In 8:35, you say that you can take the double stranded processed DNA and place it in the eukaryotic bacterial cell, did you mean prokaryotic or am I confusing the meaning of your sentence and that the eukaryotic refers to the DNA?

@zeromethanez 8 жыл бұрын

yes i think youre right

@geremy2030 7 жыл бұрын

I also think you're right

@scorpianguitar 7 жыл бұрын

For the first note, on the bottom right, the cDNA is shown to be 5' to 3' because it has been rotated 180 degrees. Notice the flat back bone is on top instead of the bottom of the bases, while the bases (perpendicular lines) are facing down. Imagine the cDNA was attached to the mRNA but then it was separated AND rotated 180 degrees to be put side by side so that they both are shown as 5' to 3'. I think that's what he did, but I am not sure.

@Sana-fp6yl 5 жыл бұрын

yes actually the dna strand synthesised first must b 3'-5' nd cdna 5'-3' so thst it becomes complementary to the parent strand and has same polarity as the mrna strand...nd actually he meant prokaryotic bacteria

@ishwaryaavvarissbn1606 2 жыл бұрын

Its easy to understand thank u.. Why it is called library !!?

@santudas_95 6 жыл бұрын

thnx sir..

@christinetj3826 4 жыл бұрын

wonderfully explained. I just have one problem with your lectures though. I have to have my inhaler next to me because you don't breath in between your explanations and that gives me short breath. It's not a joke I'm serious please take a breath sometimes. please!!

@soulofgeek2639 7 жыл бұрын

Please answer me ! I need help, why they are doing this cDNA, what are the domains of application (sorry for my english, il french )

@geremy2030 7 жыл бұрын

One application where cDNA is used is RNA-Seq: "While direct sequencing of RNA molecules is possible, most RNA-Seq experiments are carried out on instruments that sequence DNA molecules due to the technical maturity of commercial instruments designed for DNA-based sequencing. Therefore, cDNA library preparation from RNA is a required step for RNA-Seq." - RNA-Seq methods for transcriptome analysis. Hope that helps.

@soulofgeek2639 7 жыл бұрын

thanks