This step-by-step tutorial video will guide you through the process of calculating radical scavenging activity and IC50 values for various plant extracts using Microsoft Excel. The video is divided into three main parts:
1) Calculating Radical Scavenging Activity (RSA%) for Single Concentration:
-This part demonstrates how to calculate the RSA% for different plant extracts tested at a single concentration.
-The result will be presented as the mean RSA% ± standard deviation (SD).
-This method allows you to compare the radical scavenging potential between the plant extracts without using a standard compound.
2) Calculating IC50 Using RSA% vs Concentration Graph:
-In this part, you will learn how to calculate the IC50 value by plotting the RSA% against the concentration for various concentrations.
-The result will be expressed as the IC50 value.
-This method enables you to compare the activity of the plant extracts with a standard compound.
3) Calculating IC50 Using RSA% vs Concentration Graph in Triplicates:
-The final part demonstrates the calculation of IC50 using the RSA% vs concentration graph, but this time, the experiment is conducted in triplicates.
-The result will be presented as IC50 ± standard error of the mean (SEM).
-This approach allows you to compare the activity of the plant extracts with a standard compound and assess the precision of the results.
By following this tutorial, you will gain a comprehensive understanding of how to calculate radical scavenging activity and IC50 values for DPPH assays using Microsoft Excel. The video will provide you with the necessary knowledge and skills to analyze your experimental data effectively.