Gene Library

Рет қаралды 110,943

Күн бұрын

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Website video link: www.aklectures.com/lecture/gen...
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Website link: www.aklectures.com

Пікірлер: 81

@gagegomez1 4 жыл бұрын

Saving my life. I like to read my textbooks but sometimes you gotta just hit 1.75x speed and bang out 15 AKvideos

@RB-nr8rc 4 жыл бұрын

Haha true

@shabab7950 2 жыл бұрын

On allah

@fibonacci101 7 жыл бұрын

You are the reason I will do well on the MCAT. Thanks.

@erik69006 4 жыл бұрын

I NEED an update.

@daveyjones3016 3 жыл бұрын

How did you do bro ?

@sob5894 11 ай бұрын

Where are you , did you succeed .....

@NadiaMulder 7 жыл бұрын

THANK YOU, your videos are so dense and detailed.

@bohoberry0 4 жыл бұрын

Amazing! I just Love how he Draws to help visualize how it works out. I wish all instructors did this!, my instructor just talks and talks and never draws out anything so It is very hard for me to visualize how it works out. I love visuals like this! Thank you AK LECTURES! :)

@salarahmad1039 3 жыл бұрын

Sir I really like the way you speaking thank you so much.

@uzoechisamuel 3 жыл бұрын

You are just a different type of human being. You are above a genius. You are a spirit. I love you.

@AnasKhan-oj5iv 4 жыл бұрын

One of the great teachers.........i've experienced......!!!

@candyswift9088 7 жыл бұрын

Thanks for the detailed explanation about gene library.

@theresegalenkatttant 8 ай бұрын

now i finally get why it is done... thank you!!!

@akhilgajjala6018 7 жыл бұрын

Very informative and clear explanation! Thanx!

@TheVompom 8 жыл бұрын

Thank You!! Just Saved Me Again

@shoaib349 6 жыл бұрын

Explained very effectively.Thankx a lot

@darkmatter1900 7 жыл бұрын

Hey, you're very talented in pedagogics, so many thanks! :D

@akintoyepelumi2402 Жыл бұрын

You are a life saver. Thanks

@venishaasethumadhavan8773 4 жыл бұрын

Thank you. Such a detailed lecture sir.

@aseelmubarak190 7 жыл бұрын

great explanation ..u make every thing simple,thx alot😊

@nurinkusaini923 2 жыл бұрын

THIS IS VERY HELPFUL

@nyawirawaithaka4993 4 жыл бұрын

Thank you! Very well explained

@aizvass424 5 жыл бұрын

Thank you so much for the explanation

@manojkumar-ve7gp 8 жыл бұрын

very good presentation and patience....

@carlosvasconcelos4463 9 жыл бұрын

thank you man! You save all my year

@AKLECTURES 9 жыл бұрын

Carlos Vasconcelos you're welcome! :)

@iKnowYoureBusyBut... 3 жыл бұрын

I love your lectures

@630tara 5 жыл бұрын

Great explanation

@JesusLopez-nk9ck 8 жыл бұрын

your awesome dude thanks

@valerijaagicic6548 3 жыл бұрын

I love u! you saved me!

@TheBraxton1989 7 жыл бұрын

First of all congratulation for your advanced teaching skills. I wanted to ask you a specific question, you said that restriction enzymes are used to obtain separated genes. Now the question, how can, a restriction enzyme discriminate exactly the beginning and the end of a gene? A restriction enzyme cuts the DNA in specific locations in which a characteristic nucleotide arrangements (for example EcoRI cuts G/AATTC), therefore these enzyme could also cut in the middle of gene sequences, this will only produce gene fragments and not entire genes.

@alejandrocanas6744 5 жыл бұрын

My guess is that this does occur but only in a small amount relative to where we want the enzyme to cut.

@PizzoLab 4 жыл бұрын

The restriction enzyme is selected so that the DNA fragment has just one restriction site (so the "characteristic nucleotide arrangements" is repeated only once).

@nidamanurisrinu4943 2 жыл бұрын

As we already have knowledge of Nucleotide sequence of genes and recognition sequence of restriction enzymes we can select appropriate enzymes which doesn't cut through the genes(as per my knowledge)

@firephoenix8192 3 жыл бұрын

So is cDNA library and gene library same?

@heshandisanayakabiology5000 2 жыл бұрын

Superb explanation Thank you very much

@hasanafridi6019 4 жыл бұрын

my best teacher .i wanted to met him sir

@IsraeLinoy 8 жыл бұрын

Thank you! :)

@alejandrodelabarra2838 3 жыл бұрын

Un ma-es-tro. Outstanding.

@chiaramorelli3003 6 жыл бұрын

Hi, I really liked the video, but there is something I did not understand. After the transformation, when you plant out your E.Coli cells in the petri dish, do you consider all the colonies that grow up in it to build you genomic libraries or just the colonies that show to have both the plasmide and the insert (recombined vector, not just the vector)?

@manishakarwasara5492 3 жыл бұрын

Thanku so much sir.

@umar9366 2 жыл бұрын

You are lit 🔥🔥 sir.. love from budgam kashmir

@hameedpanezai6084 6 жыл бұрын

very good

@mal.v 5 жыл бұрын

First of all, this video is between many others published by this channel an incomparably great help to conquer my upcoming exams, thank you so much. But I do have one remaining question. At step 5 (~ 6:53) the bacterial cells containing the different plasmids that have grown on the nutrient agar are separately grown to produce a colony of plasmids, BUT how are they differentiated from each other on the petri dish? How do you know which library is being reproduced? Any help is appreciated!

@mal.v 5 жыл бұрын

I have actually found a logical answer in my lecture script (for those who are interested). Each of the four individual plasmids can be marked with a specific antibiotic resistance different from the others, for example the first one has an ampicillin resistance, the second one a tetracycline resistance etc. Now you can differentiate using selective media containing the separate antibiotics (multiple plates, good luck not needing to waste too many on double results lol)

@justsheerluck 4 жыл бұрын

This made the impossible, possible for me. Thank you so much. :)

@nishikantajena2511 2 жыл бұрын

Love from India 2022

@lisalasoya7979 2 жыл бұрын

I love genitics

@shoaib349 6 жыл бұрын

Please make a video lecture on Mitrochondrial biogenesis

@sammcewan9544 6 жыл бұрын

How do you individually separate the four different transformed cells in step 6? Wouldn't they look the same and be all mixed together on an agar plate?

@abinaya276 6 жыл бұрын

I had the same doubt

@abinaya276 6 жыл бұрын

I think they use radioactive labelled probes that hybridised with the desired sequence and then that bacteria containing that plasmid will be multiplied to produce libraries containing that single gene!

@basantalsayed2495 6 жыл бұрын

@mightbin 5 жыл бұрын

clone picking

@sleepyj222 5 жыл бұрын

I know it is a year later, but he kind of explains this in the next video. They use gel electrophoresis to separate by length.

@giorgisharikadzeforscience2801 Жыл бұрын

If the restriction enzyme has specific site to cut DNA, how it cuts desired region of human DNA? for example the insulin gene. Is there the palindromic sequence at the edges of desired human gene?

@louismarquez9562 4 жыл бұрын

Is there a way that we can save a picture of the dry erase board behind him? That would be really helpful when reviewing his notes

@magdabielecka8374 4 жыл бұрын

Well I just take a screenshot when he is on a left side of the screen, wait for him to walk to the right side, take another screenshot and put them into one image xD Works for me

@salarahmad1039 3 жыл бұрын

@@magdabielecka8374 same haha

@shenghuozhiyisi4577 5 жыл бұрын

Wow!

@am_aezazi 8 жыл бұрын

Exam time Savior!

@oktayhuseynov8778 8 жыл бұрын

thank you a lot) But i have 2 questions What is a difference between Genetic Library and Genomic library? And why we can not use cloning vectorr instead PCR?

@aaliagul4549 7 жыл бұрын

because PCR has two limitations.1st is, In PCR to copy a gene, the gene must be known.For unkown genes we cant make a primer in the PCR process. 2nd is, there is a limit to the length of DNA sequence that can b copied by PCR.

@munglangel9251 6 жыл бұрын

Genetic library further divides into two: viz, Genomic library and cDNA library

@persayuschung7344 3 жыл бұрын

so is lt like doing PCR in a bacteria instead of a machine?

@hamzabasil3480 7 жыл бұрын

how we can get your lecture sir

@hasanafridi6019 4 жыл бұрын

sir plz upoad a vedio on maxam gilber method of dna sequencing

@user-yt1co3gj7g 7 жыл бұрын

and what happens to the bacteria that employ plasmids are non-recombinant?

@odjen1 7 жыл бұрын

those are blue..because there beta galactosidase is still functioning...the ones that are transformed and recombinant turn white

@moizjawed5307 3 жыл бұрын

Can not the gene library be created by using PCR? It will be more time consuming. Need suggestions

@Daoro123 9 жыл бұрын

Can't we use PCR to amplify each gene instead of using plasmids?

@Misstigrine 8 жыл бұрын

+Diego Otero (student here, so believe me only if you want to :P + i'm French so excuse my English please ) You could, but PCR makes more mistakes so in the end you don't actually have that many exact copy (the longest the fragment the more errors) ... you then have to sequence the clones to be sure you have actual clones...

@mayamaya-ry3eg 7 жыл бұрын

i think if we use bacterial plasmids to amplify those genes we also would get the proteins cz we use a living cells instead of pcr?

@aaliagul4549 7 жыл бұрын

@Andreas-gh6is 6 жыл бұрын

It's way easier to grow bacteria with plasmids rather than to perform PCR. Especially if the DNA is already in a plasmid.

@zainabjaffar3450 6 жыл бұрын

PCR has a limitation like the size of the gene.